动物营养学报 >

2016 , Vol. 28 >Issue 2: 579 - 584

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.033

研究简报 Short Communications

饲粮添加脯氨酸对妊娠环江香猪繁殖性能和血浆生化参数的影响

  • 查伟 ,
  • 孔祥峰 ,
  • 谭敏捷 ,
  • 耿梅梅 ,
  • 许丽卫 ,
  • 游金明
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  • 1. 江西农业大学, 江西省动物营养重点实验室, 江西省营养饲料开发工程中心, 南昌 330045;
    2. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 长沙 410125;
    3. 中国科学院环江喀斯特农业生态试验站, 香猪研究中心, 环江 547100

收稿日期: 2015-07-28

  网络出版日期: 2016-02-19

基金资助

国家自然科学基金面上项目(31270044);中科院"西部之光"人才培养计划重点项目;留学回国人员科研启动基金

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Effects of Dietary Proline Supplementation on Reproductive Performance and Plasma Biochemical Parameters of Pregnant Huanjiang Mini-Pigs

  • ZHA Wei ,
  • KONG Xiangfeng ,
  • TAN Minjie ,
  • GENG Meimei ,
  • XU Liwei ,
  • YOU Jinming
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  • 1. Nutrition Feed Development Engineering Center of Jiangxi Province, Key Laboratory of Animal Nutrition in Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2015-07-28

  Online published: 2016-02-19

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摘要

本试验旨在研究脯氨酸代谢对妊娠环江香猪繁殖性能的影响,并探讨其可能的生理生化机制。选取48头后备环江母猪,配种15 d后随机分成3组,每组8个重复,每个重复2头猪。各组分别饲喂添加0.77% L-丙氨酸(丙氨酸组)、1% L-脯氨酸(脯氨酸组)、0.77% L-丙氨酸+0.0167%二氟甲基鸟氨酸(DFMO)(DFMO组)的试验饲粮。分别于配种第45和70天,每重复随机取1头母猪,放血处死,收集血液,用于生化参数分析;解剖母猪,记录黄体数、胎儿数和胎儿重。结果表明:妊娠第70天时,脯氨酸组窝重显著高于丙氨酸组(P<0.05);妊娠第45天时,脯氨酸组血浆谷丙转氨酶活性以及白蛋白浓度均显著低于丙氨酸组(P<0.05)。妊娠第70天与第45天相比,脯氨酸组血浆总胆固醇浓度、DFMO组血浆高密度脂蛋白和白蛋白浓度均显著降低(P<0.05)。其余指标各组间均无显著差异(P>0.05)。由此可见,饲粮添加脯氨酸可能通过改善机体氮代谢和脂肪代谢而促进妊娠环江香猪胎儿的生长发育。

关键词： 脯氨酸; 生化参数; 繁殖性能; 妊娠; 环江香猪

本文引用格式

查伟 , 孔祥峰 , 谭敏捷 , 耿梅梅 , 许丽卫 , 游金明 . 饲粮添加脯氨酸对妊娠环江香猪繁殖性能和血浆生化参数的影响[J]. 动物营养学报, 2016 , 28(2) : 579 -584 . DOI: 10.3969/j.issn.1006-267x.2016.02.033

Abstract

This experiment was conducted to investigate the effects of proline metabolism on reproductive performance in pregnant Huanjiang mini-pigs and approach its underlying physio-biochemical mechanism. Fourty-eight female Huanjiang mini-pigs were used in this study. After 15-day of service, the animals were randomly assigned into 3 groups with 8 replicates per group and 2 pigs per replicate. The pigs in the three groups were fed the experimental diets supplemented with 0.77% L-alanine (alanine group), 1% L-proline (proline group), or 0.77% L-alanine + 0.0167% 2-difluoromethylornithine (DFMO) (DFMO group) in basal diet, respectively. After 45-day and 70-day of service, one sow per replicate was weighted and killed by bloodletting. Blood samples were collected for analyzing biochemical parameters. The number of corpora luteum, fetus number and fetus weight were observed, by dissecting the sampled sows. The results showed as follows:the litter weight in proline group after 70-day of service was significantly higher compared with the alanine group (P<0.05); after 45-day of service, the plasma glutamate pyruvate transaminase activity and albumn concentration in the proline group were significantly decreased compared with the alanine group (P<0.05). After 70-day of service, the plasma total cholesterol concentrations in the proline group, and high-density lipoprotein and albumin concentrations in DFMO group were significantly decreased compared with 45-day post-service (P<0.05). The other parameters had no significant difference among three groups (P>0.05). It is concluded that dietary supplementation with proline might increase the growth and development of fetus in pregnant sows via altering the metabolism of nitrogen and lipid during pregnancy.

Key words： proline; biochemical parameter; reproductive performance; pregnancy; Huanjiang mini-pig

参考文献

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