动物营养学报 >

2016 , Vol. 28 >Issue 2: 618 - 626

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.038

研究简报 Short Communications

饲粮脂肪水平对冬毛期银狐能量代谢、血清生化指标、肝脏脂肪酸组成及肝脏型脂肪酸结合蛋白基因表达的影响

  • 张婷 ,
  • 罗婧 ,
  • 钟伟 ,
  • 孙伟丽 ,
  • 王卓 ,
  • 孙皓然 ,
  • 樊燕燕 ,
  • 邢敬亚 ,
  • 李光玉
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  • 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建实验室, 长春 130112

收稿日期: 2015-08-31

  网络出版日期: 2016-02-19

基金资助

吉林省自然科学基金项目(20140101033JC);中国农业科学院科技创新工程项目

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Effects of Dietary Fat Level on Energy Metabolism, Serum Biochemical Indices, Liver Fatty Acid Composition and Liver Type Fatty Acid Binding Protein Gene Expression of Silver Foxes During the Winter Fur-Growing Period

  • ZHANG Ting ,
  • LUO Jing ,
  • ZHONG Wei ,
  • SUN Weili ,
  • WANG Zhuo ,
  • SUN Haoran ,
  • FAN Yanyan ,
  • XING Jingya ,
  • LI Guangyu
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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Science, Changchun 130112, China

Received date: 2015-08-31

  Online published: 2016-02-19

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摘要

本试验旨在研究饲粮脂肪水平对冬毛期银狐能量代谢、血清生化指标、肝脏脂肪酸组成及肝脏型脂肪酸结合蛋白(L-FABP)基因表达的影响。选取145日龄、体重相近的健康雄性银狐50只,随机分成5组(每组10个重复,每个重复1只),分别饲喂含10%、12%、16%、20%、24%混合油脂(鸡油:豆油=1:1)的试验饲粮,试验饲粮对应的脂肪水平为12.85%(A组)、14.71%(B组)、18.72%(C组)、22.23%(D组)、26.11%(E组)。预试期为15 d,正试期为75 d。结果显示:1)饲粮脂肪水平显著或极显著影响冬毛期银狐干物质日采食量、日采食代谢能、平均日增重和代谢能增重比(P<0.05或P<0.01),而对总能消化率无显著影响(P>0.05)。2)随着饲粮脂肪水平的升高,冬毛期银狐血清甘油三酯(TG)、葡萄糖(GLU)水平呈升高趋势,且E组血清TG水平显著高于A、B组(P<0.05),A组血清GLU水平显著低于其他各组(P<0.05)。饲粮脂肪水平对血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平以及谷丙转氨酶(ALT)和谷草转氨酶(AST)活性无显著影响(P>0.05)。3)饲粮脂肪水平对冬毛期银狐肝脏脂肪酸中多不饱和脂肪酸(PUFA)、单不饱和脂肪酸(MUFA)及饱和脂肪酸(SFA)的比例有显著或极显著影响(P<0.05或P<0.01)。随着饲粮脂肪水平的升高,肝脏中PUFA和MUFA的比例升高,而SFA的比例则下降。4)随着饲粮脂肪水平的升高,冬毛期银狐肝脏中L-FABP mRNA相对表达量基本呈增加趋势,其中E组极显著高于A、B、C组(P<0.01)。由此得出,过高的饲粮脂肪水平不利于冬毛期银狐充分利用饲粮中营养物质;提高饲粮脂肪水平可增加冬毛期银狐血清TG和GLU水平,同时促进肝脏中不饱和脂肪酸的沉积;提高饲粮脂肪水平可上调冬毛期银狐肝脏中L-FABP基因的表达,进而促进脂肪酸的转运,减轻高脂饮食对肝脏造成的损伤。

关键词： 银狐; 脂肪水平; 血清生化指标; 肝脏; 脂肪酸组成; 肝脏型脂肪酸结合蛋白

本文引用格式

张婷 , 罗婧 , 钟伟 , 孙伟丽 , 王卓 , 孙皓然 , 樊燕燕 , 邢敬亚 , 李光玉 . 饲粮脂肪水平对冬毛期银狐能量代谢、血清生化指标、肝脏脂肪酸组成及肝脏型脂肪酸结合蛋白基因表达的影响[J]. 动物营养学报, 2016 , 28(2) : 618 -626 . DOI: 10.3969/j.issn.1006-267x.2016.02.038

Abstract

This experiment was conducted to study the effects of dietary fat level on energy metabolism, serum biochemical indices, liver fatty acid composition and liver type fatty acid binding protein (L-FABP) gene expression of silver foxes during the winter fur-growing period. Fifty 145-day-old healthy male silver foxes with a similar body weight were randomly divided into 5 groups with 10 replicates per group and 1 fox per replicate, and they were fed experimental diets containing 10%, 12%, 16%, 20% and 24% lipid mixture (soybean oil:chicken oil=50:50), respectively. The fat level in those experimental diets was 12.85% (group A), 14.71% (group B), 18.72% (group C), 22.23% (group D) and 26.11% (group E), respectively. The experiment was 15 days for adaptation and 75 days for trial period. The results showed as follows:1) dietary fat level significantly or extremely significantly affected the daily dry matter intake, daily metabolizable energy (ME) intake, average daily gain and ME/gain of silver foxes during the winter fur-growing period (P<0.05 or P<0.01), but there was no significant effect on gross energy digestibility (P>0.05). 2) Serum triglyceride (TG) and glucose (GLU) levels showed increasing trends with dietary fat level increasing, and the serum TG level in group E was significantly higher than that in groups A and B (P<0.05), while the serum GLU level in group A was significantly lower than that in other groups (P<0.05). There were no significant differences in serum total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) contents, and alanine transarninase (ALT) and alanine transarninase (AST) activities among groups (P>0.05). 3) Dietary fat level significantly or extremely significantly affected the proportions of liver polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and satisfied fatty acids (SFA) of silver foxes during the winter fur-growing period (P<0.05 or P<0.01). With the dietary fat level increasing, the proportions of liver PUFA and MUFA were increased, while the proportion of liver SFA was decreased. 4) The relative expression level of L-FABP mRNA in liver was up-regulated with dietary fat level increasing, and that in group E was extremely significantly higher than that in groups A, B and C (P<0.01). These results indicate that the high level of fat in the diet does not conducive to the utilization of dietary nutrients for silver foxes during the winter fur-growing period. Increasing dietary fat level can increase the levels of serum TG and GLU, and promote the unsaturated fatty acid deposition in liver of silver foxes during the winter fur-growing period. Increasing dietary fat level can also up-regulate the liver L-FABP mRNA expression of silver foxes during the winter fur-growing period,which lead to promote the transportation of fat, and then reduce the damage to the liver caused by high fat diet.

Key words： silver foxes; fat level; serum biochemical indices; liver; fatty acid composition; liver type fatty acid binding protein

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