动物营养学报 >

2016 , Vol. 28 >Issue 4: 1105 - 1113

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.04.019

水产营养 Aquaculture Nutrition

饲料脂肪水平对大鳞副泥鳅幼鱼生长性能、消化酶活性及抗氧化能力的影响

  • 曾本和 ,
  • 廖增艳 ,
  • 吴双 ,
  • 赵杰锌 ,
  • 向枭 ,
  • 王强
展开
  • 西南大学荣昌校区水产系, 淡水鱼类资源与生殖发育教育部重点实验室, 水产科学重庆市市级重点实验室, 重庆 402460

收稿日期: 2015-11-15

  网络出版日期: 2016-04-11

基金资助

重庆市基础与前沿研究计划项目(cstc2013jcyjA80033);西南大学青年基金资助;西南大学信豚水产学生创新基金

收起

Effects of Dietary Lipid Level on Growth Performance, Digestive Enzyme Activities and Antioxidant Ability of Juvenile Paramisgurnus dabryanus

  • ZENG Benhe ,
  • LIAO Zengyan ,
  • WU Shuang ,
  • ZHAO Jiexin ,
  • XIANG Xiao ,
  • WANG Qiang
Expand
  • Key Laboratory of Aquatic Science of Chongqing, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Department of Fisheries in Rongchang Compust, Southwest University, Chongqing 402460, China

Received date: 2015-11-15

  Online published: 2016-04-11

Fold

摘要

本试验旨在研究饲料脂肪水平对大鳞副泥鳅幼鱼生长性能、消化酶活性及抗氧化能力的影响。选用初始体重为(0.15±0.01) g的大鳞副泥鳅幼鱼1620尾,随机分成6组,每组3个重复,每个重复90尾鱼,分别投喂脂肪水平为2.50%、4.49%、6.48%、8.47%、10.46%和12.45%的试验饲料,养殖时间为60 d。结果表明:大鳞副泥鳅幼鱼的特定生长率(SGR)在饲料脂肪水平为8.47%时最高,为4.99%/d,除与10.46%组差异不显著(P>0.05)外,显著高于其余各试验组(P<0.05);饲料系数(FCR)在饲料脂肪水平为8.47%时最低,为1.09,除与10.46%组差异不显著(P>0.05)外,显著低于其余各试验组(P<0.05)。通过二次多项式回归分析可知,大鳞副泥鳅幼鱼SGR、FCR最优时饲料脂肪水平分别为9.18%、9.00%。随着饲料脂肪水平的升高,大鳞副泥鳅幼鱼肝胰脏及前肠蛋白酶、脂肪酶活性均呈先升高后降低的变化趋势,且均在8.47%组达到最高值,除与10.46%组差异不显著(P>0.05)外,显著高于其余各试验组(P<0.05);肝胰脏和前肠淀粉酶的活性则呈先下降后逐渐趋于稳定的趋势。随着饲料脂肪水平的升高,肝胰脏超氧化物歧化酶、过氧化氢酶活性均呈先升高后降低的变化趋势,且均在8.47%组达到最高值,除与10.46%组差异不显著(P>0.05)外,显著高于其余各试验组(P<0.05)。由此得出,适宜的饲料脂肪水平可促进大鳞副泥鳅幼鱼的生长,提高其蛋白酶与脂肪酶活性和抗氧化能力。本试验条件下,综合考虑大鳞副泥鳅幼鱼生长性能、消化酶活性和抗氧化能力,其饲料的适宜脂肪水平为8.47%~10.46%。

关键词： 大鳞副泥鳅幼鱼; 饲料脂肪水平; 生长性能; 消化酶活性; 抗氧化能力

本文引用格式

曾本和 , 廖增艳 , 吴双 , 赵杰锌 , 向枭 , 王强 . 饲料脂肪水平对大鳞副泥鳅幼鱼生长性能、消化酶活性及抗氧化能力的影响[J]. 动物营养学报, 2016 , 28(4) : 1105 -1113 . DOI: 10.3969/j.issn.1006-267x.2016.04.019

Abstract

This experiment was aimed to investigate the effects of dietary lipid level on growth performance, digestive enzyme activities and antioxidant ability of juvenile Paramisgurnus dabryanus. Six diets were formulated by containing 2.50%, 4.49%, 6.48%, 8.47%, 10.46% and 12.45% lipid, respectively. A total of 1620 juvenile Paramisgurnus dabryanus with an initial body weight of(0.15±0.01) g were randomly divided into 6 groups with 3 replicates per group and 90 fish per replicate. Each diet was fed one group of 90 fish for 60 d. The results showed that specific growth rate(SGR) had the highest value(4.99%/d) when the dietary lipid level was 8.47%, and it was significantly higher than that in other experimental groups(P<0.05) except the 10.46% group(P>0.05); feed conversion ratio(FCR) had the lowest value(1.09) when the dietary lipid level was 8.47%, and it was significantly lower than that in other experimental groups(P<0.05) except the 10.46% group(P>0.05). By quadratic polynomial regression analysis, dietary lipid levels were 9.18% and 9.00% when the juvenile Paramisgurnus dabryanus had the optimal SGR and FCR, respectively. With the dietary lipid level increasing, the hepatopancreas and foregut protease and lipase activities of fish were increased at first and then decreased, and all them had the highest values when the dietary lipid level was 8.47%, which were significantly higher than those in other experimental groups(P<0.05) except the 10.46% group(P>0.05); the amylase activities in hepatopancreas and foregut were decreased at first and then tend to stabilized. Both of the superoxide dismutase(SOD) and catalase(CAT) activities in hepatopancreas were increased at first and then decreased with the dietary lipid level increasing, and all them had the highest values when the dietary lipid level was 8.47%, which were significantly higher than those in other experimental groups(P<0.05) except the 10.46% group(P>0.05). Above results show that suitable dietary lipid level can improve the growth, protease and lipase activities and antioxidant ability of juvenile Paramisgurnus dabryanus. We suggest that the optimal dietary lipid level for juvenile Paramisgurnus dabryanus is 8.47% to 10.46% through comprehensive analysis of growth performance, digestive enzyme activities and antioxidant ability.

Key words： juvenile Paramisgurnus dabryanus; growth performance; dietary lipid level; digestive enzyme activities; antioxidant ability

参考文献

[1] CHOU B S,SHIAU S Y.Optimal dietary lipid level for growth of juvenile hybrid tilapia,Oreochromis niloticus×Oreochromis aureus[J].Aquaculture,1996,143(2):185-195.  
[2] HUANG C H,LIN W Y,CHU J H.Dietary lipid level influences fatty acid profiles,tissue composition,and lipid peroxidation of soft-shelled turtle,Pelodiscus sinensis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2005,142(3):383-388.  
[3] SONG X A,HIRATA T,KAWAI T,et al.Volatile compounds in the hepatic and muscular tissues of common carp,japanese flounder,spanish mackerel and skipjack[J].Developments in Food Science,2004,42:209-222.
[4] 向枭,周兴华,陈建,等.饲料脂肪水平对白甲鱼幼鱼生长性能、体组成和血清生化指标的影响[J].动物营养学报,2013,25(8):1805-1816.
[5] DE SILVA S S,GUNASEKERA R M,SHIM K F.Interactions of varying dietary protein and lipid levels in young red tilapia:evidence of protein sparing[J].Aquaculture,1991,95(3/4):305-318.
[6] 韩雨哲,姜志强,任同军,等.氧化鱼油与棕榈油对花鲈肝脏抗氧化酶及组织结构的影响[J].中国水产科学,2010,17(4):798-806.
[7] 麦康森.水产动物营养与饲料学[M].2版.北京:中国农业出版社,2011:41-53.
[8] 王敏,王卫民,鄢建龙.泥鳅和大鳞副泥鳅年龄与生长的比较研究[J].水利渔业,2001,21(1):7-9.
[9] 赵振山,高贵琴,印杰,等.泥鳅和大鳞副泥鳅营养成分分析[J].水利渔业,1999,19(2):16-17.
[10] 陈清泉,宋光泉,胡良成,等.洞庭湖区优势""""泥鳅""""品种营养品质的研究初报[J].水生生物学报,1989,13(4):384-386.
[11] 王玉新,郑玉珍,王锡荣,等.大鳞副泥鳅的生物学特性及养殖技术[J].河北渔业,2012(11):23-25,43.
[12] 中山大学生物系生化微生物教研室.生化技术导论[M].北京:人民教育出版社,1979:26-29.
[13] 常雅宁,王志友,刘金秀,等.两种连苯三酚自氧化法测定超氧化物歧化酶的比较[J].药物分析杂志,2001,21(5):328-331.
[14] 孔德胜,王晓然,李文君,等.小鼠组织中过氧化氢酶的活性与年龄的关系[J].生物学杂志,2012,29(3):11-13,10.
[15] 付世建,谢小军,张文兵,等.南方鲇的营养学研究:Ⅲ.饲料脂肪对蛋白质的节约效应[J].水生生物学报,2001,25(1):70-75.
[16] TAKEUCHI T,SHⅡNA Y,WATANABE T.Suitable protein and lipid levels in diet for fingerlings of red sea bream Pagrus major[J].The Japanese Society of Fisheries Science,1991,57(2):293-299.  
[17] HALVER J E,HARDY R W.Fish nutrition[M].London:Academic Press,1989.
[18] 刘波,唐永凯,俞菊华,等.饲料脂肪对翘嘴红鲌生长、葡萄糖激酶和葡萄糖-6-磷酸酶活性与基因表达的影响[J].中国水产科学,2008,15(6):1024-1033.
[19] 赵巧娥,朱邦科,沈凡,等.饲料脂肪水平对鳡幼鱼生长、体成分及血清生化指标的影响[J].华中农业大学学报,2012,31(3):357-363.
[20] 王朝明,罗莉,张桂众,等.饲料脂肪水平对胭脂鱼幼鱼生长、体组成和抗氧化能力的影响[J].淡水渔业,2010,40(5):47-53.
[21] CHATZIFOTIS S,PANAGIOTIDOU M,PAPAIOANNOU N,et al.Effect of dietary lipid levels on growth,feed utilization,body composition and serum metabolites of meagre(Argyrosomus regius) juveniles[J].Aquaculture,2010,307(1/2):65-70.
[22] 向枭,周兴华,陈建,等.日粮脂肪水平对翘嘴红鲌幼鱼生长性能和体组成的影响[J].动物营养学报,2009,21(3):411-416.
[23] LOU G,XU J H,TENG Y J,et al.Effects of dietary lipid levels on the growth,digestive enzyme,feed utilization and fatty acid composition of Japanese sea bass(Lateolabrax japonicus L.) reared in freshwater[J].Aquaculture Research,2010,41(2):210-219.  
[24] MAUGLE P D,SIMPSON K L,DESHIMARU O,et al.Effect of short-necked clam(Venerupis philippinarum) diets on shrimp(Penaeus japonicus) growth and digestive enzyme activities[J].Bulletin of the Japanese Society for the Science of Fish,1982,48(12):1759-1764.  
[25] 向枭,周兴华,陈建,等.饲料蛋白水平及鱼粉蛋白含量对齐口裂腹鱼生长、体组成及消化酶活性的影响[J].中国粮油学报,2012,27(5):74-80.
[26] 王爱民,吕富,杨文平,等.饲料脂肪水平对异育银鲫生长性能、体脂沉积、肌肉成分及消化酶活性的影响[J].动物营养学报,2010,22(3):625-633.
[27] 向枭,周兴华,陈建,等.饲料中脂肪含量对翘嘴红鲌幼鱼消化酶活性的影响[J].北京水产,2008(5):35-38.
[28] 王朝明.岩原鲤幼鱼蛋白需求和胭脂鱼幼鱼脂肪需求研究[J].硕士学位论文.重庆:西南大学,2011.
[29] MORAIS S,CONCEIÇÀO L E C,RØNNESTAD I,et al.Dietary neutral lipid level and source in marine fish larvae:effects on digestive physiology and food intake[J].Aquaculture,2007,268(1/2/3/4):106-122.
[30] 韩光明,王爱民,徐跑,等.饲料中脂肪水平对吉富罗非鱼幼鱼成活率、肌肉成分及消化酶活性的影响[J].上海海洋大学学报,2010,19(4):469-474.
[31] 尾崎久雄.鱼类消化生理(上册)[M].上海:上海科学技术出版社,1985.
[32] BARAGI V,LOVELL R T.Digestive enzyme activities in striped bass from first feeding through larva development[J].Transactions of the American Fisheries Society,1986,115(3):478-484.  
[33] PEDERSEN B H,NILSSEN E M,HJELMELAND K.Variations in the content of trypsin and trysinogen in larval herring(Clupea harengus) digesting copepod nauplii[J].Marine Biology,1987,94(2):171-181.  
[34] GANGADHARA B,MANDEESHA M C,VARGHESE T J,et al.Effect of varying protein and lipid levels on the growth of rohu,Labeo rohita[J].Asian Fisheries Science,1997,10(2):139-147.
[35] GÜLŞ,BELGE-KURUTAŞ E,YILDIZ E,et al.Pollution correlated modifications of liver antioxidant systems and histopathology of fish(Cyprinidae) living in Seyhan Dam Lake,Turkey[J].Environment International,2004,30(5):605-609.  
[36] 张春暖,王爱民,刘文斌,等.饲料脂肪水平对梭鱼脂肪沉积、脂肪代谢酶及抗氧化酶活性的影响[J].中国水产科学,2013,20(1):108-115.
[37] 孙阳,姜志强,李艳秋,等.饲料脂肪水平对红鳍东方鲀幼鱼肝脏抗氧化酶活力及组织结构的影响[J].广东海洋大学学报,2013,33(3):27-32.
[38] 曾端,麦康森,艾庆辉.脂肪肝病变大黄鱼肝脏脂肪酸组成、代谢酶活性及抗氧化能力的研究[J].中国海洋大学学报,2008,38(4):542-546.
[39] 施兆鸿,岳彦峰,彭士明,等.饲料脂肪水平对褐菖鲉血清生化指标、免疫及抗氧化酶活力的影响[J].中国水产科学,2013,20(1):101-107.
[40] 周本宏,刘刚,胡先明.罗布麻对红细胞膜脂质过氧化损伤的保护作用[J].广东药学院学报,2004,20(5):506-508.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2