试验旨在探讨绞股蓝内生真菌JY25胞外多糖(JY25P)对蛋雏鸡生长性能和肠黏膜免疫功能的影响。将300羽健康的1日龄三黄蛋雏鸡,随机分为5组(每组设3个重复,每个重复20羽):Ⅰ组为灌服无菌水的空白对照组;Ⅱ组、Ⅲ组和Ⅳ组分别为灌服0.04%、0.08%和0.12% JY25P的试验组;Ⅴ组为灌服0.08%黄芪多糖的阳性对照组。结果表明:1)与Ⅰ组相比,Ⅳ组蛋雏鸡平均日增重显著提高了15.86%(P<0.05),料重比极显著降低了24.13%(P<0.01)。2)与Ⅰ组相比,试验各阶段,Ⅲ组、Ⅳ组、Ⅴ组均能显著提高蛋雏鸡血清中新城疫抗体效价(P<0.05)。3)与Ⅰ组相比,在15日龄和20日龄,Ⅳ组可以极显著增加肠黏膜白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)含量(P<0.01),而对分泌型免疫球蛋白A(SIgA)和IFN-γ/IL-4的影响不显著(P>0.05)。结果提示:灌服0.12% JY25P能改善蛋雏鸡的生长性能,提高特异性新城疫抗体效价,增强非特异性肠黏膜IL-4和IFN-γ的分泌,同时维持机体的免疫平衡,因此,该多糖具有较好的应用前景。

The experiment was to investigate the effects of extracellular polysaccharide extracted from Gynostemma pentaphyllum endophytic fungi JY25(JY25P for short) on the growth performance and immunologic function in the intestinal mucosa of laying chicks. Three hundred healthy one-day-old Sanhuang laying chicks were randomly divided into 5 groups with 3 replicates in each group and 20 chicks per replicate. Chicks in the blank control group(group Ⅰ) were drenched with sterile water, and in group Ⅱ, Ⅲ and Ⅳ were drenched with 0.04%, 0.08% and 0.12% JY25P respectively, while in group Ⅴ(positive control) were drenched with 0.08% Astragalus polysaccharide. The results showed as follows:compared with group Ⅰ, 1) the average daily gain in group Ⅳ was significantly improved by 15.86%(P<0.05) and the feed/gain in group Ⅳ was extremely significantly reduced by 24.13%(P<0.01); 2) during all periods of the experiment, the serum Newcastle disease antibody titer in group Ⅲ, Ⅳ and Ⅴ was significantly increased(P<0.05); 3) at 15 and 20 days of age, the contents of interleukin-4(IL-4) and interferon-γ(IFN-γ) in intestinal mucosal of chicks in group Ⅳ were extremely significantly increased(P<0.01), while the secretory immunoglobulin A(SIgA) content or the proportion of IFN-γ to IL-4 was not changed significantly(P>0.05). The conclusion is that chicks drenched with 0.12% JY25P get excellent performances on growth and Newcastle disease antibody titer, and 0.12% JY25P can enhance the secreted contents of IL-4 and IFN-γ, which represented non-specific immune function in intestinal mucosal, and also maintain the body's immune balance. Thus it can be seen that JY25P has the potential application prospect.