颈静脉灌注精氨酸对泌乳中期奶牛泌乳性能和牛乳酪蛋白合成的影响

周刚; 王梦芝; 张军; 丁洛阳; 张鑫; 徐巧云

doi:10.3969/j.issn.1006-267x.2016.04.031

动物营养学报 >

2016 , Vol. 28 >Issue 4: 1199 - 1207

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.04.031

研究简报 Short Communications

颈静脉灌注精氨酸对泌乳中期奶牛泌乳性能和牛乳酪蛋白合成的影响

周刚 ,
王梦芝 ,
张军 ,
丁洛阳 ,
张鑫 ,
徐巧云

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1. 扬州大学动物科学与技术学院, 扬州 225009;
2. 扬州市扬大康源乳业有限公司, 扬州 225004

收稿日期: 2015-10-22

网络出版日期: 2016-04-11

基金资助

江苏省自然科学基金基础研究项目(BK20141270,BK20151312);江苏省优势学科(PAPD)

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Effects of Arginine Infusion through Jugular Vein on Milk Performance and Casein Synthesis of Mid-Lactation Cows

ZHOU Gang ,
WANG Mengzhi ,
ZHANG Jun ,
DING Luoyang ,
ZHANG Xin ,
XU Qiaoyun

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1. College of Animal and Technology, Yangzhou University, Yangzhou 225009, China;
2. Yangzhou city Yangda Kangyuan Dairy Co., Ltd., Yangzhou 225004, China

Received date: 2015-10-22

Online published: 2016-04-11

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摘要

本文旨在研究颈静脉灌注精氨酸对泌乳中期奶牛泌乳性能和牛乳酪蛋白合成的影响。将6头体重、胎次、泌乳期、泌乳量和体况基本一致的荷斯坦奶牛随机分为3组(每组2头):酪蛋白模式组(对照组)、精氨酸灌注组、丙氨酸等氮组(与精氨酸灌注组等氮),采用3×3复拉丁方试验设计,每期22 d(7 d灌注期+15 d间隔期),测定其泌乳性能、酪蛋白含量以及酪蛋白基因的表达情况。结果表明:1)灌注的第5天精氨酸灌注组的乳蛋白及乳中非脂固形物含量均显著高于酪蛋白模式组(P<0.05);第6天精氨酸灌注组的乳脂率显著高于丙氨酸等氮组(P<0.05)。2)酪蛋白模式组的α-酪蛋白含量显著低于其他2组(P<0.05);β-酪蛋白含量在3组间没有显著的差异(P>0.05);κ-酪蛋白含量则以精氨酸灌注组为最高,显著高于其他2组(P<0.05)。3)精氨酸灌注组αs1-酪蛋白基因(CSN1S1)、αs2-酪蛋白基因(CSN1S2)的表达量显著高于其他2组(P<0.05)。综上,灌注精氨酸提高了乳蛋白中α-酪蛋白和κ-酪蛋白含量,以及CSN1S1、CSN1S2在奶牛乳腺组织的表达量,有利于牛乳中乳蛋白率和乳品质的提高。

关键词： 颈静脉灌注; 精氨酸; 泌乳性能; 酪蛋白

本文引用格式

周刚 , 王梦芝 , 张军 , 丁洛阳 , 张鑫 , 徐巧云 . 颈静脉灌注精氨酸对泌乳中期奶牛泌乳性能和牛乳酪蛋白合成的影响[J]. 动物营养学报, 2016 , 28(4) : 1199 -1207 . DOI: 10.3969/j.issn.1006-267x.2016.04.031

Abstract

The objective of this study was to investigate the effects of arginine infusion through jugular vein on milk performance and casein synthesis of mid-lactation cows. Six healthy lactating cows at similar lactation stages with similar body weight, parity, milk yield and body condition were divided into 3 groups(2 cows for each group) in the 3×3 Latin square trail, which were casein model group(control group), arginine infusion group and alanine iso-nitrogen group(equal nitrogen amount to arginine infusion group). Each period lasted for 22 days with 7-day infusion plus 15-day interval per period. Milk performance, casein contents and casein gene expressions were detected. The results showed as follows:1) on day 5 of infusion, arginine infusion group was significantly higher than casein model group in the contents of milk protein and milk non-fat milk solids(P<0.05); on day 6, arginine infusion group was significantly higher in milk fat percentage compared to alanine iso-nitrogen group(P<0.05). 2) α-casein content in casein model group was significantly lower than that in the other 2 groups(P<0.05); β-casein content had no significant difference among groups(P>0.05); κ-casein content of arginine infusion group was the highest, and was significantly higher than that in the other 2 groups(P<0.05). 3) Arginine infusion group had significantly higher expression levels in αs1-casein gene(CSN1S1) and αs2-casein gene(CSN1S2) compared to those in the other 2 groups(P<0.05). It is concluded that arginine infusion increases the contents of α-casein and κ-casein in milk, as well as expression levels of CSN1S1 and CSN1S2 in mammary tissue from dairy cows, which is contributed to the improvements of milk protein percentage and milk quality.

Key words： jugular vein infusion; arginine; milk performance; casein

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