水产营养 Aquaculture nutrition

饲料中胆汁酸添加水平对齐口裂腹鱼幼鱼生长性能、形体指标及体成分的影响

  • 郑宗林 ,
  • 曾本和 ,
  • 向枭 ,
  • 周兴华 ,
  • 陈建 ,
  • 吕光俊 ,
  • 朱成科 ,
  • 李代金 ,
  • 任胜杰
展开
  • 1. 西南大学荣昌校区水产系, 淡水鱼类资源与生殖发育教育部重点实验室, 水产科学重庆市市级重点实验室, 重庆 402460;
    2. 西藏自治区农牧科学院水产研究所, 拉萨 850000

收稿日期: 2016-02-04

  网络出版日期: 2016-08-17

基金资助

重庆市基础与前沿研究计划项目(cstc2013jcyjA80033);西南大学青年基金项目

Effects of Bile Acid Supplemental Level on Growth Performance, Physical Indices and Body Composition of Juvenile Schizothorax prenanti

  • ZHENG Zonglin ,
  • ZENG Benhe ,
  • XIANG Xiao ,
  • ZHOU Xinghua ,
  • CHEN Jian ,
  • LYU Guangjun ,
  • ZHU Chengke ,
  • LI Daijin ,
  • REN Shengjie
Expand
  • 1. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aqucatic Science of Chongqing, Department of Fisheries in Rongchang Compust, Southwest University, Chongqing 402460, China;
    2. Tibet Academy of Agricultural and Animal Husbandry Seiences, Fisheries Research Institute, Lasa 850000, China

Received date: 2016-02-04

  Online published: 2016-08-17

摘要

本试验旨在探索饲料中胆汁酸添加水平对齐口裂腹鱼幼鱼生长性能、形体指标及体成分的影响。以360尾健康、平均体重为(12.74±0.14)g的齐口裂腹鱼幼鱼为试验对象,随机分为4组,每组3个重复,每个重复30尾。4组试验鱼分别投喂添加0(对照组)、75、150、300 mg/kg胆汁酸的试验饲料,养殖时间为70 d。结果表明:随着胆汁酸添加水平的增加,齐口裂腹鱼幼鱼的增重率(WGR)、特定生长率(SGR)、饲料蛋白质效率(PER)均呈先升高后趋于稳定的变化趋势,饲料系数(FCR)则呈先降低后趋于稳定的变化趋势。各胆汁酸添加组的WGR、SGR、PER均显著高于对照组(P<0.05),而FCR则显著低于对照组(P<0.05)。当胆汁酸添加水平为150 mg/kg时,WGR、SGR均达到最大值,分别为226.63%和1.69%/d;当胆汁酸添加水平为300 mg/kg时,PER达到最大值,为1.64%;FCR则在胆汁酸添加水平为75 mg/kg时有最小值,为1.56。通过二次回归分析可知,WGR、SGR、PER和FCR最佳时的胆汁酸添加水平分别为186.83、192.86、166.67和183.33 mg/kg。随着胆汁酸添加水平的增加,齐口裂腹鱼幼鱼的肥满度(CF)呈先升高后趋于稳定的变化趋势,脏体比(VSI)、肝体比(HSI)则呈先降低后趋于稳定的变化趋势。各胆汁酸添加组的CF显著高于对照组(P<0.05),而VSI、HSI则显著低于对照组(P<0.05)。随着胆汁酸添加水平的增加,齐口裂腹鱼全鱼及肌肉粗蛋白质含量呈先升高后趋于稳定的变化趋势,全鱼、肌肉及肝胰脏中粗脂肪含量则呈先降低后趋于稳定的变化趋势,但全鱼及肌肉中水分、粗灰分含量则无显著变化(P>0.05)。由此得出,饲料中添加胆汁酸能有效地提高齐口裂腹鱼幼鱼的饲料利用率,促进其生长,改善其形体指标,减少其肌肉及肝脏中脂肪的沉积。综合考虑,齐口裂腹鱼饲料中胆汁酸适宜的添加水平为166.67~192.86 mg/kg。

本文引用格式

郑宗林 , 曾本和 , 向枭 , 周兴华 , 陈建 , 吕光俊 , 朱成科 , 李代金 , 任胜杰 . 饲料中胆汁酸添加水平对齐口裂腹鱼幼鱼生长性能、形体指标及体成分的影响[J]. 动物营养学报, 2016 , 28(8) : 2423 -2430 . DOI: 10.3969/j.issn.1006-267x.2016.08.013

Abstract

This experiment was conducted to study the effects of bile acid supplemental level on the growth performance, physical indices and body composition of juvenile Schizothorax prenanti. A total of 360 healthy juvenile Schizothorax prenanti with the average body weight of (12.74±0.14) g were randomly divided into four groups with three replicates of 30 fish per replicate. Each group was fed a experimental diet containing either 0 (control group), 75, 150 or 300 mg/kg bile acid for 70 days. The results showed as follows:with bile acid supplemental level raising, the weight gain rate (WGR), specific growth rate(SGR), protein efficiency ratio (PER) had a trend of going up first and then leveled off. Conversely, the feed conversation ratio (FCR) was firstly decreased and then to stabilization. The WGR, SGR and PER in bile acid supplemental groups were significantly higher than those in control group (P<0.05), while the FCR in bile acid supplemental groups was significantly lower than that in control group (P<0.05). The WGR and SGR were all the highest (226.63% and 1.69%/d, respectively) when the bile acid supplemental level was 150 mg/kg, and the PER was the highest (1.64%) when the bile acid supplemental level was 300 mg/kg, while the FCR was the lowest (1.56) when the bile acid supplemental level was 75 mg/kg. Based on square regression analysis, the suitable bile acid supplemental level was 186.83, 192.86, 166.67 and 183.33 mg/kg, respectively, when WGR, SGR, PER and FCR of juvenile Schizothorax prenanti were optimum. With bile acid supplemental level raising, the condition factor (CF) was initially increased and then leveled off, the viscerasomatic index (VSI) and hepaticsomatic index (HSI) were initially increased and then decreased. The CF in bile acid supplemental groups was significantly higher than that in control group (P<0.05), while the VSI and HSI in bile acid supplemental groups were significantly lower than those in control group (P<0.05). The crude protein content of whole body and muscle was initially increased and then to stabilization with bile acid supplemental level raising, while the crude lipid content of whole body, muscle and hepatopancreas was initially decreased and then leveled off. There were no significant differences in moisture and crude ash contents of whole body and muscle with bile acid supplemental level raising (P>0.05). Results of above show that bile acid supplementation can improve feed efficiency, promote growth, improve physical indices and reduce fat deposition in muscle and liver of juvenile Schizothorax prenanti. After comprehensive consideration, the suitable bile acid supplemental level in juvenile Schizothorax prenanti diet is 166.67 to 192.86 mg/kg.

参考文献

[1] 温安祥,曾静康,何涛.齐口裂腹鱼肌肉的营养成分分析[J].水利渔业,2003,23(1):13-15.
[2] 王纪亭,宋憬愚,李海涛,等.乳化剂对建鲤生长及血液生化指标的影响[J].大连水产学院学报,2009,24(3):257-260.
[3] 刘兆阳,王建平,刘宁.乳化剂对肉鸡肠道菌群及免疫功能的影响[J].中国畜牧兽医,2013,40(8):60-63.
[4] REINHART G A,MAHAN D C,CERA K R.Effect of group size and feeding regimen on nutrient digestibility studies with weanling pigs[J].Journal of Animal Science,1989,67(10):2684-2691.
[5] PULLEN D L,POLIN D.Effect of bile acids and diet composition on lipid absorption in chickens with cannulated bile ducts[J].Poultry Science,1984,63(10):2020-2026.  
[6] 汪军涛.饲料中食盐、DL-肉碱复合物、胆汁酸对斑点叉尾生长和部分生理机能、体色因子的影响[D].硕士学位论文.苏州:苏州大学,2008.
[7] 王恒.不同脂肪源及胆汁酸对罗氏沼虾生长性能及脂肪酸组成的影响[D].硕士学位论文.扬州:扬州大学,2010.
[8] 周书耘,刘永坚,梁海鸥,等.饲料中添加胆汁酸对军曹鱼生长及体组成的影响[J].南方水产,2010,6(4):20-25.
[9] AOAC.Official methods of analysis of AOAC International[M].16th ed.Arlington,VA:Association of Analytical Communities,1995:1094.
[10] CHOU B S,SHIAU S Y.Optimal dietary lipid level for growth of juvenile hybrid tilapia,Oreochromis niloticus×Oreochromis aureus[J].Aquaculture,1996,143(2):185-195.  
[11] 胡田恩,王玲,张春晓,等.饲料中添加胆汁酸对牛蛙生长性能、体组成和营养物质表观消化率的影响[J].水生生物学报,2015,39(4):677-685.
[12] DESHIMARU O,KUROKI K,YONE Y.Suitable levels of lipids and ursodesoxycholic acid in diet for yellowtail[J].Bulletin of the Japanese Society of Scientific Fisheries,1982,48(9):1265-1270.  
[13] 孙建珍,王际英,马晶晶,等.不同脂肪水平下添加胆汁酸对大菱鲆(Scophthalmus maximus)幼鱼生长、体组成和脂肪代谢的影响[J].海洋与湖沼,2014,45(3):617-625.
[14] MAITA M,TACHIKI H,KAIBARA A,et al.Pharmacological effect of ursodeoxycholic acid in juvenile eel[J].Nippon Suisan Gakkaishi,1996,62(1):129-130.  
[15] 颉志刚,牛翠娟.可利康对虹鳟生长的影响[J].饲料研究,2002(10):22-25.
[16] 林仕梅,叶元土,罗莉.胆汁酸添加剂对异育银鲫生长的影响[J].广东饲料,2003,12(3):14-15.
[17] 谭永刚,魏文志,曾党胜.饲料中添加胆汁酸对异育银鲫生长性能的影响[J].广东饲料,2008,17(1):25-26.
[18] 刘敬盛,杨玉芝,王君荣,等.胆汁酸营养功能及作用机制的研究进展[J].中国饲料,2010(6):35-37,43.
[19] KIM S K,MATSUNARI H,TAKEUCHI T,et al.Effect of different dietary taurine levels on the conjugated bile acid composition and growth performance of juvenile and fingerling Japanese flounder Paralichthys olivaceus[J].Aquaculture,2007,273(4):595-601.  
[20] 向枭,周兴华,陈建,等.乳酸对鲫鱼的生长及饲料利用率的影响[J].中国饲料,2009(11):40-41.
[21] 孙逊,杨刚毅.胆汁酸对糖脂代谢影响的研究现状[J].国际内分泌代谢杂志,2008,28(5):323-325.
[22] 黄炳山,李宝山,张利民,等.胆汁酸对大菱鲆幼鱼生长、脂肪代谢酶及血清生化的影响[J].上海海洋大学学报,2015,24(5):737-744.
[23] 向枭,周兴华,陈建,等.饲料脂肪水平对白甲鱼幼鱼生长性能、体组成和血清生化指标的影响[J].动物营养学报,2013,25(8):1805-1816.
[24] CHATZIFOTIS S,PANAGIOTIDOU M,PAPAIOANNOU N,et al.Effect of dietary lipid levels on growth,feed utilization,body composition and serum metabolites of meagre (Argyrosomus regius) juveniles[J].Aquaculture,2010,307(1/2):65-70.
[25] WATANABE M,HOUTEN S M,MATAKI C,et al.Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation[J].Nature,2006,439(7075):484-489.  
文章导航

/