研究简报 Short communications

应激和饲粮能量水平对肉仔鸡肝脏脂肪合成能力的影响

  • 蔡元丽 ,
  • 林海
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  • 1. 齐鲁师范学院生命科学学院, 济南 250013;
    2. 山东农业大学动物科技学院, 泰安 271018

收稿日期: 2016-02-02

  网络出版日期: 2016-08-17

基金资助

国家自然科学基金(30771573)

Effects of Stress and Dietary Energy Level on Fatty Synthesis in Liver of Broiler Chickens

  • CAI Yuanli ,
  • LIN Hai
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  • 1. College of Life Sciences, Qilu Normal University, Jinan 250013, China;
    2. Department of Animal Science, Shandong Agricultural University, Taian 271018, China

Received date: 2016-02-02

  Online published: 2016-08-17

摘要

本研究旨在探讨应激和饲粮能量水平对肉仔鸡肝脏脂肪酸合成酶(fatty acid synthase, FAS)活性以及腺苷一磷酸激活的蛋白激酶(AMP-activated protein kinase, AMPK)和固醇调节元件结合蛋白-1(sterol-regulatory element binding proteins-1, SREBP-1)基因mRNA表达量的影响,从而阐明应激造成肉仔鸡脂肪肝的原因,并寻找缓解应激的方法。本研究共有4个试验。前2个试验分别针对3~9日龄和28~34日龄的肉仔鸡,分别给予皮质酮和高、低能量水平饲粮的处理,试验结束后取肝脏,测定FAS活性。第3个试验选取7日龄体重相近的爱拔益加雄性肉鸡108只,随机分为3组:应激组(注射地塞米松)、对照组(注射生理盐水)和配对组(注射生理盐水,采食量与应激组前1 d的相同)。连续注射7 d。14日龄取肝脏,测定肝脏中甘油三酯的含量以及AMPKSREBP-1 mRNA的表达量。最后1个试验给予3~9日龄肉仔鸡皮质酮和葡萄糖饮水处理,试验结束测定肝脏FAS活性。结果发现:1)皮质酮处理极显著提高了3~9日龄肉仔鸡肝脏FAS的活性(P<0.01),对28~34日龄肉仔鸡的影响也有相似的趋势(P=0.051 2)。2)与配对组相比,地塞米松处理显著增加了肝脏中甘油三酯的含量(P<0.05),显著提高了肝脏中AMPK mRNA的表达量(P<0.05);且地塞米松处理使得肝脏中SREBP-1 mRNA的表达量显著高于对照组和配对组(P<0.05)。3)给应激组的肉仔鸡饮水中添加葡萄糖能显著降低肝脏FAS活性(P<0.05)。结果表明:皮质酮处理会提高肉仔鸡肝脏FAS的活性,地塞米松处理则可显著提高肝脏SREBP-1 mRNA的表达量,且能激活AMPK,而葡萄糖具有缓解应激的作用。

本文引用格式

蔡元丽 , 林海 . 应激和饲粮能量水平对肉仔鸡肝脏脂肪合成能力的影响[J]. 动物营养学报, 2016 , 28(8) : 2512 -2520 . DOI: 10.3969/j.issn.1006-267x.2016.08.023

Abstract

The objective of this study was to explore fatty acid synthase (FAS) activity, expressions of AMP-activated protein kinase (AMPK) and sterol-regulatory element binding proteins-1 (SREBP-1) mRNA in liver of broiler chickens treated by glucocorticoid or dietary energy level in order to explain why stress can raise the hepatic triglyceride and look for ways to relieve stress. There were four trials in this study. In the first and the second trials, broiler chickens fed high and low different energy level of diets were challenged with corticosterone from 3 to 9 days of age and 28 to 34 days of age, respectively. At the end of the two trials, livers were removed for measuring FAS activity. In the third trial, 108 male Arbor Acres chickens with 7 days of age were divided into 3 groups injected with dexamethasone (stress group), saline (control group) and saline (a pair-fed group, with the same feed intake compared to stress group) for 7 d, respectively. At the 14 days of age, the liver was removed to determine the triglyceride content and expressions of AMPK and SREBP-1 mRNA. In the last trial, chickens aged from 3 to 9 days were treated with corticosterone and drinking water supplemented with glucose, and FAS activity was measured in the end. The results showed as follows:1) FAS activity was significantly increased by corticosterone treatment in chickens of 3 to 9 days old (P<0.05), and had the increasing trend in chickens of 28 to 34 days old (P=0.051 2). 2) Compared with the pair-fed group, dexamethasone administration resulted in enhanced triglyceride content in liver (P<0.05), and the AMPK mRNA expression level in liver was significantly up-regulated (P<0.05). The SREBP-1 mRNA expression level in liver of chickens treated with dexamethasone were significantly higher than those of control and pair-fed groups (P<0.05). 3) Glucose supplementation could significantly decrease the FAS activity in liver of chickens during stress (P<0.05). In conclusion, corticosterone treatment can increase the FAS activity in liver of broiler chickens, dexamethasone administration can increase SREBP-1 mRNA expression level in liver and activate AMPK, and glucose can relieve the harm of stress.

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