饲粮铜含量对牦牛体外瘤胃发酵的影响

薛艳锋; 郝力壮; 刘书杰; 柴沙驼; 张晓卫; 赵索南

doi:10.3969/j.issn.1006-267x.2016.08.033

动物营养学报 >

2016 , Vol. 28 >Issue 8: 2599 - 2606

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.08.033

研究简报 Short communications

饲粮铜含量对牦牛体外瘤胃发酵的影响

薛艳锋 ,
郝力壮 ,
刘书杰 ,
柴沙驼 ,
张晓卫 ,
赵索南

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1. 省部共建三江源生态与高原农牧业国家重点实验室, 青海省高原放牧家畜动物营养与饲料科学重点实验室, 青海高原牦牛研究中心, 青海大学畜牧兽医科学院, 西宁 810016;
2. 海北州畜牧兽医科学研究所, 海晏 810200

收稿日期: 2016-02-27

网络出版日期: 2016-08-17

基金资助

“973”国家重点基础研究发展计划项目（2012CB722906）；“十二五”国家科技支撑计划项目（2012BAD13B01）；国家国际科技合作专项项目（2015DFG31870）；公益性行业（农业）科研专项（201203008）；教育部长江学者和创新团队发展计划项目（IRT13074）；青海省重大科技平台建设项目（2013-Z-Y03）

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Effects of Dietary Copper Content on Rumen Fermentation of Yaks in Vitro

XUE Yanfeng ,
HAO Lizhuang ,
LIU Shujie ,
CHAI Shatuo ,
ZHANG Xiaowei ,
ZHAO Suonan

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1. State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center, Qinghai Academy of Science and Veterinary Medicine of Qinghai University, Xining 810016, China;
2. Academy of Science and Veterinary Medicine of Haibei Prefecture, Haiyan 810200, China

Received date: 2016-02-27

Online published: 2016-08-17

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摘要

为了探究牦牛饲粮中微量元素铜的适宜含量，本试验以甘氨酸铜作为添加形式，以牦牛饲粮为底物进行体外瘤胃发酵，底物铜含量分别为5.0、10.0、15.0、20.0、25.0 mg/kg，共发酵48 h。测定发酵后的产气量、瘤胃发酵指标及消化酶活力。结果表明：当底物铜含量为15.0 mg/kg时，干物质消化率（DMD），微生物蛋白质（MCP）、丙酸、异丁酸、丁酸、异戊酸、戊酸、总挥发性脂肪酸浓度，脂肪酶（LPS）、胰蛋白酶（TYS）、纤维素酶（CLS）活力均达到最大值，分别为63.858%、4.289 g/L、24.475 mmol/L、0.470 mmol/L、8.977 mmol/L、1.159 mmol/L、1.607 mmol/L、81.583 mmol/L、0.504 U/mL、84.167 U/mL、79.956 U/mL，乙酸/丙酸最低为2.045；当铜含量为10.0 mg/kg时，MCP、乙酸浓度达到最大值，分别为4.289 g/L、51.075 mmol/L，且其他指标也都处于较高水平。综合得出，在体外条件下，牦牛饲粮推荐铜含量为10.0～15.0 mg/kg。

关键词： 牦牛; 甘氨酸铜; 体外产气技术; 消化酶活力; 挥发性脂肪酸

本文引用格式

薛艳锋 , 郝力壮 , 刘书杰 , 柴沙驼 , 张晓卫 , 赵索南 . 饲粮铜含量对牦牛体外瘤胃发酵的影响[J]. 动物营养学报, 2016 , 28(8) : 2599 -2606 . DOI: 10.3969/j.issn.1006-267x.2016.08.033

Abstract

To find an optimal content of copper in diet for yaks, cupric glycinate was used as additive, and diet for yaks was used as a substrate for in vitro rumen fermentation in the present study. Copper contents in substrates were designed as 5.0, 10.0, 15.0, 20.0, 25.0 mg/kg, respectively. The fermentation lasted for 48 h. After fermentation, gas production, rumen fermentation parameters and digestive enzyme activities were measured. The results showed as follows:when the content of copper in substrate was 15.0 mg/kg, dry matter digestibility (DMD), the concentrations of microbial protein (MCP), propionic acid, isobutyric acid, butyric acid, isovaloric acid, valeric acid and total volatile fatty acids, as well as the activities of lipidase, trypsinase, cellulase reached the highest, which were 63.858%, 4.289 g/L, 24.475 mmol/L, 0.470 mmol/L, 8.977 mmol/L, 1.159 mmol/L, 1.607 mmol/L, 81.583 mmol/L, 0.504 U/mL, 84.167 U/mL and 79.956 U/mL, respectively; when the content of copper in substrate was 10.0 mg/kg, acetic acid/propionic acid reached the lowest, which was 2.045; when the content of copper was 10.0 mg/kg, MCP and acetic acid concentrations reached the highest, which were 4.289 g/L and 51.075 mmol/L, respectively, and the values of other indexes were also on high level. Therefore, under in vitro conditions, the copper content recommended in yak's diet is between 10.0 and 15.0 mg/kg.

Key words： yak; cupric glycinate; in vitro gas production technique; digestive enzyme activity; volatile fatty acid

参考文献

[1] WIENER G,HAN J L,LONG R J.The yak[M].3^nd ed.Bangkok:RAP Publication,2003:1-18.
[2] 胡令浩.牦牛营养研究论文集[M].西宁:青海人民出版社,1997.
[3] 韩兴泰,胡令浩,谢敖云,等.粗饲条件下生长牦牛能量代谢的估测[J].青海畜牧兽医杂志,1992,22(2):21-22.
[4] 薛白,柴沙驼,刘书杰.生长期牦牛蛋白质需要量的研究[J].青海畜牧兽医杂志,1994,24(4):1-4.
[5] 刘宗平.现代动物营养代谢病学[M].北京:化学工业出版社,2003:132-142.
[6] 杨凤.动物营养学[M].2版.北京:中国农业出版社,2003:116-117.
[7] 袁施彬,何平,陈代文.微量元素铜的营养生理功能和促生长机制[J].饲料工业,2004,25(7):23-26.
[8] 郭宝林.不同饲料铜源对绵羊铜代谢及纤维素降解影响的研究[D].博士学位论文.北京:中国农业大学,2004.
[9] 杨文正,章世元,林在光,等.动物矿物质营养[M].北京:中国农业出版社,1996:6-9.
[10] 吴建设,呙于明.微量元素铜的营养与免疫研究进展[J].国外畜牧科技,1999,26(1):5-9.
[11] 李文立,黄新成.微量元素添加剂对牛育肥效果试验报告[J].莱阳农学院学报,1994,11(4):313-315.
[12] 杨正德.不同铜添加水平对肉牛铜代谢及肥育性能的影响[J].中国畜牧杂志,2000,36(3):14-16.
[13] 杨红建.肉牛微量元素铜的营养[J].饲料研究,1999(8):1-3.
[14] PERRY J M,ZHAO Y,MARLETTA M A,et al.Cu²⁺ and Zn²⁺ inhibit nitric-oxide synthase through an interaction with the reductase domain[J].The Journal of Biological Chemistry,2000,275(19):14070-14076.

[15] 马长星.奶牛对微量元素铜的营养需要研究[J].畜牧兽医科技信息,2010(11):43.
[16] MENKE K H,RAAB L,SALEWSKI A,et al.The Estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J].Journal of Agriculture Science,1979,93(1):217-222.

[17] 中华人民共和国国家质量监督检验检疫总局.GB/T 13885-2003动物饲料中钙、铜、铁、镁、锰、钾、钠和锌含量的测定原子吸收光谱法[S].北京:中国标准出版社,2004.
[18] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37.
[19] 曹庆云,周武艺,朱贵钊,等.气相色谱测定羊瘤胃液中挥发性脂肪酸方法研究[J].中国饲料,2006(24):26-28.
[20] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011:139-141.
[21] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.
[22] 哈斯花.体外发酵产气技术在饲料营养价值评定中的作用[J].当代畜禽养殖业,2013(1):15-19.
[23] 高天爽,孙海霞,谢小来,等.日粮中高粱替代玉米对绵羊瘤胃发酵的影响[J].黑龙江畜牧兽医,2014(9):7-10.
[24] 张海涛,王加启,卜登攀,等.纳豆枯草芽孢杆菌对犊牛断奶前后瘤胃发酵和酶活的影响[J].中国畜牧兽医,2009,36(12):5-11.
[25] 刘彩娟,孙满吉,孙金艳,等.饲粮中添加复合益生菌对奶牛瘤胃发酵及纤维素酶活的影响[J].动物营养学报,2011,23(5):821-827.
[26] MOHARRERY A,BRASK M,WEISBJERG M R.The effect of different physical forms of rapeseed as a fat supplement on the activity of some enzymes in the duodenal chyme of dairy cows[J].Journal of Animal and Feed Sciences,2014,23(4):293-298.
[27] 王杰.小尾寒羊、滩羊生长性能及其主要消化生理参数的比较[D].硕士学位论文.杨凌:西北农林科技大学,2008.
[28] GOODRICH R D,TILLMAN A D.Effects of sulfur and nitrogen sources and copper levels on the metabolism of certain minerals by sheep[J].Journal of Animal Science,1966,25(2):484-491.

[29] SAXENA K K,RANJHAN S K.A note on the effect of cobalt and copper supplementation on in vivo cellulose digestion by nylon-bag technique in Hariana calves[J].Indian Journal of Animal Science,1978,48:833-835.
[30] LENG R A,NOLAN J Y.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,1984,67(5):1072-1089.

[31] SLYTER L L.Monensin and dichloroacetamide influences on methane and volatile Fatty Acid production by rumen bacteria in vitro[J].Applied and Environmental Microbiology,1979,37(2):283-288.
[32] COLEMAN G S,SANDFORD D C.The uptake and utilization of bacteria,amino acids and nucleic acid components by the rumen ciliate Eudiplodinium maggii[J].Journal of Applied Bacteriology,1979,47(3):409-419.

[33] GRAY G M.Starch digestion and absorption in nonruminants[J].The Journal of Nutrition,1992,122(1):172-177.
[34] 赵国琦,贾亚红,陈小连,等.不同NDF/NFE比的日粮对山羊瘤胃发酵参数影响的研究[J].中国畜牧杂志,2006,42(13):29-33.

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