研究简报 Short communications

地顶孢霉培养物对大鼠生长性能、血清与肝脏抗氧化及免疫指标的影响

  • 李洋 ,
  • 林聪 ,
  • 许文斌 ,
  • 张幸怡 ,
  • 王一臻 ,
  • 高红 ,
  • 窦秀静 ,
  • 郝小燕 ,
  • 张永根
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  • 东北农业大学动物科学技术学院, 哈尔滨 150030

收稿日期: 2016-02-14

  网络出版日期: 2016-08-17

基金资助

国家奶牛产业技术体系(CARS-37)

Effects of Acremonium terricola Culture on Growth Performance, Antioxidant and Immune Indexes of Serum and Liver in Sprague Dawley Rats

  • LI Yang ,
  • LIN Cong ,
  • XU Wenbin ,
  • ZHANG Xingyi ,
  • WANG Yizhen ,
  • GAO Hong ,
  • DOU Xiujing ,
  • HAO Xiaoyan ,
  • ZHANG Yonggen
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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2016-02-14

  Online published: 2016-08-17

摘要

本试验旨在研究地顶孢霉培养物对大鼠生长性能、血清与肝脏抗氧化以及免疫指标的影响。选用体重相近的SD大鼠40只,随机分成5组,每组8个重复,每个重复1只大鼠。5组分别是对照组和4个不同地顶孢霉培养物剂量组(10、50、250和1 250 mg/kg BW组),连续灌胃21 d。结果表明:地顶孢霉培养物极显著提高大鼠的平均日增重(P<0.01);提高了大鼠肝脏指数、胸腺指数;提高了大鼠血清中总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)和葡萄糖(GLU)含量,降低了谷丙转氨酶(ALT)、谷草转氨酶(AST)活力和低密度脂蛋白(LDL)含量;提高了血清和肝脏中谷胱甘肽过氧化物酶(GSH-Px)活力和总抗氧化能力(T-AOC),降低丙二醛(MDA)含量,同时提高了肝脏中的总超氧化物歧化酶(T-SOD)含量;提高了血清免疫球蛋白A、免疫球蛋白G和免疫球蛋白M含量,同时提高血清和肝脏中白介素-4(IL-4)含量,降低白介素-1β(IL-1β)和白介素-17(IL-17)含量。综合各项指标,地顶孢霉培养物能够提高SD大鼠平均日增重,改善血清生化指标,提高大鼠机体抗氧化能力和免疫能力,其中以250 mg/kg BW的添加量为最佳。

本文引用格式

李洋 , 林聪 , 许文斌 , 张幸怡 , 王一臻 , 高红 , 窦秀静 , 郝小燕 , 张永根 . 地顶孢霉培养物对大鼠生长性能、血清与肝脏抗氧化及免疫指标的影响[J]. 动物营养学报, 2016 , 28(8) : 2657 -2666 . DOI: 10.3969/j.issn.1006-267x.2016.08.039

Abstract

This study was aimed to investigate the effects of Acremonium terricola culture on growth performance, antioxidant and immune indexes of serum and liver in Sprague Dawley rats. Fourty Sprague Dawley rats with near weight were randomly divided into 5 groups with 8 replicates per group and 1 rat per replicate. One was control group and others were experimental groups with different doses of Acremonium terricola culture (10, 50, 250 and 1 250 mg/kg BW, respectively). Acremonium terricola culture was given corresponding medicines for 21 days via gavage. The results showed as follows:Acremonium terricola culture significantly improved average daily gain of rats (P<0.01); improved liver and thymus indexes; improved serum total protein (TP), albumin (ALB), globulin (GLB) and glucose (GLU) contents, decreased serum activities of alanine transaminase (ALT) and aspertate transaminase (AST), and serum content of low-density lipoprotein (LDL); improved glutathione peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC), decreased malondialdehyde (MDA) content of serum and liver, and improved superoxide dismutase (T-SOD) activity of liver; increased serum contents of immunoglobulins A, M and G, increased interleukin-4 (IL-4) content of serum and liver, and decreased contents of interleukin-1β (IL-1β) and interleukin-17 (IL-17) of serum and liver. In conclusion, Acremonium terricola culture can improve average daily gain, biochemical indexes, antioxidant and immunity of serum and liver in Sprague Dawley rats, and the optimal supplemental level is 250 mg/kg body weight.

参考文献

[1] 梁宗琦.我国虫草属真菌研究开发的现状及思考[J].食用菌学报,2001,8(2):53-62.
[2] 肖建辉,方宁,肖瑜,等.古尼拟青霉胞外多糖的免疫抑制活性[J].中药材,2004,27(3):192-195.
[3] 黄建忠,梁宗琦,刘爱英.粉被虫草(Cordyceps pruinosa Petch)无性型对苏云金杆菌(Bacillus thuringiensis subsp. galleria Heimpel)抗紫外辐射的保护效应[J].西南农业学报,1992,5(2):63-67.
[4] 魏建忠,张玮,李郁,等.地顶孢霉培养物对保育仔猪生产性能及免疫水平的影响[J].中国畜牧兽医,2009,36(2):33-35.
[5] 孙汉巨,李晓祥,丁琦,等.虫草饲料添加剂对蛋鸭生产性能及鸭蛋品质的影响[J].安徽农业科学,2011,39(6):3618-3620.
[6] 李侠,黄欢,田小朋,等.地顶孢霉菌丝体对免疫性肝纤维化大鼠的保护作用及其可能机制的研究[J].安徽医科大学学报,2012,47(2):145-149.
[7] 程洁,陈双林.顶孢霉发酵液提取物清除自由基能力的研究[J].食品工业科技,2010,31(11):155-157.
[8] DENG B,WANG Z P,TAO W J,et al.Effects of polysaccharides from mycelia of Cordyceps sinensis on growth performance,immunity and antioxidant indicators of the white shrimp Litopenaeus vannamei[J].Aquaculture Nutrition,2015,21(2):173-179.  
[9] 朴美子,王晓东.蛹虫草黄豆对小鼠的抗氧化及免疫作用[J].中国食品学报,2014,14(5):32-37.
[10] HAN J C,QU H X,WANG J G,et al.Effects of fermentation products of Cordyceps militaris on growth performance and bone mineralization of broiler chicks[J].Journal of Applied Animal Research,2015,43(2):236-241.  
[11] WANG C L,CHIANG C J,CHAO Y P,et al.Effect of Cordyceps militaris waster medium on production performance,egg traits and egg yolk cholesterol of laying hens[J].The Journal of Poultry Science,2015,52(3):188-196.  
[12] KOH J H,SUH H J,AHN T S.Hot-water extract from mycelia of Cordyceps sinensis as a substitute for antibiotic growth promoters[J].Biotechnology Letters,2003,25(7):585-590.  
[13] KWON N H,KIM S H,BAE W K,et al.Antimicrobial activity of Lactobacillus reuteri against major food-borne pathogens[J].Journal of Food Hygiene and Safety,2001,16:264-273.
[14] 孔祥峰,尹富贵,刘合军,等.早期断奶仔猪生理生化参数和脏器指数的变化[J].中国实验动物学报,2006,14(4):298-302.
[15] 孙连海,郭明月.蛹虫草发酵乳饮料对小鼠免疫力的影响[J].中国食物与营养,2014,20(6):67-69.
[16] WANG M,MENG X Y,YANG R L,et al.Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J].Carbohydrate Polymers,2012,89(2):461-466.  
[17] 夏新山,袁书林,杨元青,等.中草药添加剂及不同饲粮类型对生长育肥猪血液生化指标的影响[J].动物科学与动物医学,2003,20(12):38-39.
[18] 李洋,潘春方,李春雷,等.不同比例的湿玉米纤维饲料对奶牛瘤胃发酵特性和血液生化指标的影响[J].中国畜牧杂志,2014,50(1):44-48.
[19] 李宁军,李惊子,辛岗.黄芪当归合剂对肾病综合征大鼠脂蛋白脂酶和卵磷脂胆固醇酰基转移酶的影响[J].中国中西医结合杂志,1999,19(8):484-486.
[20] 沃兴德,崔小强,唐利华.姜黄素对食饵性高脂血症大鼠血浆脂蛋白代谢相关酶活性的影响[J].中国动脉硬化杂志,2003,11(3):223-226.
[21] 倪淑华,李秀花,刘雪枫,等.生姜对大鼠血脂代谢影响的研究[J].中国老年学杂志,2006,26(1):98-99.
[22] YOUNG I S,WOODSIDE J V.Antioxidants in health and disease[J].Journal of Clinical Pathology,2001,54(3):176-186.  
[23] DONG C H,ZHAO J M,SONG L S,et al.The immune responses in Chinese mitten crab Eriocheir sinensis challenged with double-stranded RNA[J].Fish & Shellfish Immunology,2009,26(3):438-442.  
[24] YAMAGUCHI Y,KAGOTA S,NAKAMURA K,et al.Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis[J].Phytotherapy Research,2000,14(8):647-649.  
[25] GRASSO P J,SCHOLZ R W,ERSKINE R J,et al.Phagocytosis,bactericidal activity,and oxidative metabolism of milk neutrophils from dairy cows fed selenium-supplemented and selenium-deficient diets[J].American Journal of Veterinary Research,1990,51(2):269-274.
[26] POLITIS I,HIDIROGLOU M,BATRA T R,et al.Effects of vitamin E on immune function of dairy cows[J].American Journal of Veterinary Research,1995,56(2):179-184.
[27] KOH J H,YU K W,SUH H J,et al.Activation of macrophages and the intestinal immune system by an orally administered decoction from cultured mycelia of Cordyceps sinensis[J].Bioscience,Biotechnology,and Biochemistry,2002,66(2):407-411.  
[28] ZHANG J L,YU Y C,ZHANG Z F,et al.Effect of polysaccharide from cultured Cordyceps sinensis on immune function and anti-oxidation activity of mice exposed to 60Co[J].International Immunopharmacology,2011,11(12):2251-2257.  
[29] RÖCKEN M,RACKE M,SHEVACH E M.IL-4-induced immune deviation as antigen-specific therapy for inflammatory autoimmune disease[J].Immunology Today,1996,17(5):225-231.  
[30] KANDA T,TAKAHASHI T.Interleukin-6 and cardiovascular diseases[J].Japanese Heart Journal,2004,45(2):183-193.  
[31] DE RHAM C,FERRARI-LACRAZ S,JENDLY S,et al.The proinflammatory cytokines IL-2,IL-15 and IL-21 modulate the repertoire of mature human natural killer cell receptors[J].Arthritis Research & Therapy,2007,9(6):R125.
[32] HUANG X L,WANG Y J,YAN J W,et al.Role of anti-inflammatory cytokines IL-4 and IL-13 in systemic sclerosis[J].Inflammation Research,2015,64(3/4):151-159.
[33] AMORNRIT W,SANTIYANONT R.Effect of amaranthus on advanced glycation end-products induced cytotoxicity and proinflammatory cytokine gene expression in SH-SY5Y cells[J].Molecules,2015,20(9):17288-17308.  
[34] MOORE E E,PRESNELL S,GARRIGUES U,et al.Expression of IL-17B in neurons and evaluation of its possible role in the chromosome 5q-linked form of Charcot-Marie-Tooth disease[J].Neuromuscular Disorders,2002,12(2):141-150.  
[35] CHEN J R,YEN J H,LIN C C,et al.The effects of Chinese herbs on improving survival and inhibiting anti-ds DNA antibody production in lupus mice[J].The American Journal of Chinese Medicine,1993,21(3/4):257-262.
[36] KUO Y C,TSAI W J,SHIAO M S,et al.Cordyceps sinensis as an immunomodulatory agent[J].The American Journal of Chinese Medicine,1996,24(2):111-125.  
[37] KUO Y C,TSAI W J,WANG J Y,et al.Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis[J].Life Sciences,2001,68(9):1067-1082.  
[38] WU Y L,SUN H X,QIN F,et al.Effect of various extracts and a polysaccharide from the edible mycelia of Cordyceps sinensis on cellular and humoral immune response against ovalbumin in mice[J].Phytotherapy Research,2006,20(8):646-652.  
[39] NG T B,WANG H X.Pharmacological actions of Cordyceps,a prized folk medicine[J].Journal of Pharmacy and Pharmacology,2005,57(12):1509-1519.  
[40] 董佳亮,张蕾.虫草素对环磷酰胺所致骨髓抑制小鼠CenpB表达的影响[J].山东大学学报:医学版,2015,53(7):24-28.
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