猪胰高血糖素样肽-2通过细胞外信号调节激酶1/2调节仔猪空肠上皮细胞紧密连接蛋白表达

  • 齐珂珂 ,
  • 孙雨晴 ,
  • 万晶 ,
  • 邓波 ,
  • 门小明 ,
  • 吴杰 ,
  • 徐子伟
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  • 浙江省农业科学院畜牧兽医研究所, 杭州 310021

收稿日期: 2016-06-01

  网络出版日期: 2016-12-17

基金资助

现代农业产业技术体系(CARS-36);浙江省自然科学基金项目(LY15C170002)

Porcine Glucagon-Like Peptide-2 Regulates Tight Junction Protein Expressions in Porcine Small Intestinal Epithelial Cell through Extracellular Regulated Kinase 1/2 Signaling Pathway

  • QI Keke ,
  • SUN Yuqing ,
  • WAN Jing ,
  • DENG Bo ,
  • MEN Xiaoming ,
  • WU Jie ,
  • XU Ziwei
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  • Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2016-06-01

  Online published: 2016-12-17

摘要

本试验以体外培养的仔猪空肠上皮细胞(IPEC-J2)为研究对象,研究了猪胰高血糖素样肽-2(pGLP-2)对紧密连接蛋白表达的调节及其信号传导机制。培养液中分别添加10-9 mol/L pGLP-2(pGLP-2组)和10-9 mol/L pGLP-2、10 μmol/L U0126(pGLP-2+U0126组),对照组不添加以上试剂,每组3个重复,每个重复1个培养孔。测定IPEC-J2胞质紧密黏连蛋白-1(ZO-1)、occludin、claudin-1以及细胞外信号调节激酶1/2(ERK1/2)蛋白表达量。结果表明:与对照组相比,IPEC-J2细胞培养液中添加pGLP-2显著增加了ZO-1、occludin、claudin-1以及p42-ERK1/2、p44-ERK1/2的蛋白表达量(P<0.05);与pGLP-2组相比,在IPEC-J2细胞培养液中加入ERK1/2的抑制剂U0126,显著降低了ZO-1、occludin、claudin-1以及p42-ERK1/2、p44-ERK1/2的蛋白表达量(P<0.05)。综合得出,ERK1/2通路是pGLP-2调控肠道上皮细胞紧密连接蛋白表达的一条重要信号通路。

本文引用格式

齐珂珂 , 孙雨晴 , 万晶 , 邓波 , 门小明 , 吴杰 , 徐子伟 . 猪胰高血糖素样肽-2通过细胞外信号调节激酶1/2调节仔猪空肠上皮细胞紧密连接蛋白表达[J]. 动物营养学报, 2016 , 28(12) : 3912 -3916 . DOI: 10.3969/j.issn.1006-267x.2016.12.024

Abstract

Porcine small intestinal epithelial cell from jejunum (IPEC-J2) was used to study the regulation of porcine glucagon-like peptide-2 (pGLP-2) on protein expressions of tight junction and its signaling pathway. Culture mediums were supplemented without (control group) or with 10-9 mol/L pGLP-2 (pGLP-2 group), and 10-9 mol/L pGLP-2 and 10 μmol/L U0126 (pGLP-2+U0126 group), respectively. Each group had 3 replicates with 1 culture pore per replicate. Protein expressions of zonula occludens-1 (ZO-1), occluding, claudin-1 and extracellular regulated kinase 1/2 (ERK1/2) in cytoplasm of IPEC-J2 were determined. The results showed as follows:compared with control group, the supplementation of pGLP-2 in culture medium of IPEC-J2 significantly increased protein expressions of ZO-1, claudin-1, occludin, p42-ERK1/2 and p44-ERK1/2 (P<0.05); compared with pGLP-2 group, the supplementation of U0126, an inhibitor of ERK1/2, in culture medium of IPEC-J2 significantly decreased protein expressions of the above proteins (P<0.05). The results suggest that ERK1/2 pathway is an important signaling pathway of pGLP-2 regulating tight junction protein expressions in intestinal epithelial cells.

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