动物营养学报 >

2016 , Vol. 28 >Issue 12: 3925 - 3934

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.026

饲料硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响

  • 胡俊茹 ,
  • 王国霞 ,
  • 孙育平 ,
  • 陈冰 ,
  • 曹俊明 ,
  • 黄燕华 ,
  • 卓丽欣
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  • 1. 广东省农业科学院动物科学研究所, 广州 510640;
    2. 广东省动物育种与营养公共实验室, 广州 510640;
    3. 广东省畜禽育种与营养研究重点实验室, 广州 510640

收稿日期: 2016-06-24

  网络出版日期: 2016-12-17

基金资助

2011年广东省农业科技项目（高生物量富硒酵母的制备及其在养殖中的应用）

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Effects of Dietary Selenium Content on Growth Performance, Antioxidant Capacity and Lipid Metabolism Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

  • HU Junru ,
  • WANG Guoxia ,
  • SUN Yuping ,
  • CHEN Bing ,
  • CAO Junming ,
  • HUANG Yanhua ,
  • ZHUO Lixin
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2016-06-24

  Online published: 2016-12-17

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摘要

本试验旨在研究饲料中硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响。选用初始体重为（2.12±0.01）g的黄颡鱼幼鱼450尾，随机分为6组，每组设3个重复，每个重复25尾幼鱼，分别投喂硒含量<0.05（对照组）、0.15、0.23、0.35、0.47和0.96 mg/kg的6种等氮等脂试验饲料，饲养期为8周。结果显示：黄颡鱼的增重率随着饲料硒含量的升高呈现先升高后降低的趋势，在饲料硒含量为0.23 mg/kg时达到最高，显著高于对照组（P<0.05）。饲料系数的变化趋势与增重率相反，在饲料硒含量为0.23 mg/kg时达到最低，显著低于对照组（P<0.05）。存活率各组均为100%。全鱼粗蛋白质含量在饲料硒含量为0.35 mg/kg的组最高，显著高于对照组和饲料硒含量为0.23 mg/kg的组（P<0.05）。对照组全鱼粗脂肪含量显著低于饲料硒含量为0.15、0.47和0.96 mg/kg的组（P<0.05），而对照组全鱼水分含量则显著高于饲料硒含量为0.15、0.35、0.47和0.96 mg/kg的组（P<0.05）。血浆胆固醇含量在饲料硒含量为0.23 mg/kg的组最低，显著低于对照组（P<0.05）。全鱼硒含量和肝脏谷胱甘肽过氧化物酶（GPx）活性随着饲料硒含量的增加而升高，二者与饲料硒含量呈正剂量效应关系。肝脏超氧化物歧化酶（SOD）活性随着饲料硒含量由<0.05 mg/kg升高到0.47 mg/kg表现为先升高再降低，而后在饲料硒含量为0.96 mg/kg时又出现升高。对照组肝脏脂蛋白脂酶（LPL）和脂肪酸合成酶（FAS）mRNA的相对表达量显著低于饲料硒含量为0.23 mg/kg的组（P<0.05）。以增重率为评价指标，通过非线性回归分析计算出黄颡鱼幼鱼饲料中硒的适宜含量为0.20 mg/kg。

关键词： 硒; 黄颡鱼幼鱼; 生长性能; 抗氧化能力; 脂肪代谢基因表达

本文引用格式

胡俊茹 , 王国霞 , 孙育平 , 陈冰 , 曹俊明 , 黄燕华 , 卓丽欣 . 饲料硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响[J]. 动物营养学报, 2016 , 28(12) : 3925 -3934 . DOI: 10.3969/j.issn.1006-267x.2016.12.026

Abstract

This experiment was conducted to investigate the effects of dietary selenium content on growth performance, antioxidant capacity and lipid metabolism gene expression of juvenile yellow catfish (Pelteobagrus fulvidraco). A total of 450 juvenile yellow catfish with the initial body weight of (2.12±0.01) g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The fish in those groups were fed 6 isonitrogenous and isolipid experimental diets containing <0.05 (control group), 0.15, 0.23, 0.35, 0.47 and 0.96 mg/kg selenium for 8 weeks, respectively. The results showed as follows:the weight gain rate (WGR) increased firstly and decreased subsequently with dietary selenium content increasing, and the highest value appeared at the 0.23 mg/kg selenium group, which was significantly higher than that in control group (P<0.05). The feed conversion rate (FCR) exhibited an opposite trend to the WGR, and the 0.23 mg/kg selenium group had the lowest value, which was significantly lower than that in control group (P<0.05). The survival ratio in all groups was 100%. The highest whole body crude protein content was found in the 0.35 mg/kg selenium group, which was significantly higher than that in control group and 0.23 mg/kg selenium group (P<0.05). The whole body crude lipid content in control group was significantly lower than that in 0.15, 0.47 and 0.96 mg/kg selenium groups (P<0.05), but the whole body moisture content in control group was significantly higher than that in 0.15, 0.35, 0.47 and 0.96 mg/kg selenium groups (P<0.05). The plasma cholesterol (CHO) content in 0.23 mg/kg selenium group was the lowest, and was significantly lower than that in control group (P<0.05). The whole body selenium content and liver glutathione peroxidase (GPx) activity were enhanced with dietary selenium content increasing, and presented a dose-response relationship. The liver superoxide dismutase (SOD) activity was firstly increased and then decreased with dietary selenium content increased from <0.05 to 0.47 mg/kg, and then was increased again when dietary selenium content was 0.96 mg/kg. The relative expression levels of lipoprotein lipase (LPL) and lipoprotein lipase (FAS) mRNA in control group were significantly lower than those in 0.23 mg/kg selenium group (P<0.05). Using WGR as the evaluation index, the suitable dietary selenium content of juvenile yellow catfish is estimated to be 0.20 mg/kg by nonlinear regression analysis.

Key words： selenium; juvenile yellow catfish; growth performance; antioxidant capacity; lipid metabolism gene expression

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