早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子和小肠黏膜免疫相关细胞的影响

吴婷婷; 韩冰; 刘振; 何周瑞; 杨开伦

doi:10.3969/j.issn.1006-267x.2016.12.028

动物营养学报 >

2016 , Vol. 28 >Issue 12: 3943 - 3951

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.028

早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子和小肠黏膜免疫相关细胞的影响

吴婷婷 ,
韩冰 ,
刘振 ,
何周瑞 ,
杨开伦

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1. 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052;
2. 新疆畜牧科学院生物技术研究中心, 新疆维吾尔自治区动物生物技术重点开放实验室, 农业部草食家畜遗传育种与繁殖重点实验室, 乌鲁木齐 830000

收稿日期: 2016-06-02

网络出版日期: 2016-12-17

基金资助

2011国家自然科学基金（31172233）

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Effects of Early Administration of Ruminal Fluid on Plasma Immunoglobins and Cytokines, and Mucosal Immunity-Associated Cells in Small Intestine in Lambs

WU Tingting ,
HAN Bing ,
LIU Zhen ,
HE Zhourui ,
YANG Kailun

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agriculture University, Urumqi 830052, China;
2. Key Laboratory of Animal Biotechnology of Xinjiang, Key Laboratory of Genetics, Breeding and Reproduction of Grass-Feeding Animal, Ministry of Agriculture, Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi 830000, China

Received date: 2016-06-02

Online published: 2016-12-17

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摘要

本试验旨在研究早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子含量和小肠黏膜免疫相关细胞数量的影响。选取12只特克赛尔（Texel）初生羔羊，随机分成2组，每组6只。试验组羔羊出生后连续补喂瘤胃液14 d，每天1次，对照组羔羊补喂等量生理盐水。羔羊7、14、21和28日龄时，颈静脉采血并分离血浆；28日龄时，屠宰收集肠道内容物。测定血浆中免疫球蛋白[免疫球蛋白A（IgA）和免疫球蛋白G（IgG）]、细胞因子[白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）]，肠道内容物中IgG和免疫球蛋白Fc片段（Fc）含量。结果表明：试验组21日龄血浆中IgA和IgG含量显著或极显著低于对照组（P<0.05或P<0.01），其他日龄组间差异不显著（P>0.05）；试验组血浆中IL-1β和IL-6含量较对照组高，但差异不显著（P>0.05）；试验组血浆TNF-α含量在14日龄时显著高于对照组（P<0.05），在其他日龄差异不显著（P>0.05）；试验组羔羊血浆IFN-γ含量低于对照组，但差异不显著（P>0.05）；给新生羔羊补喂瘤胃液对28日龄羔羊肠道内容物中IgG和Fc含量无显著影响（P>0.05）；试验组肠道上皮内淋巴细胞数量较对照组增加，尤其在回肠，与对照组差异显著（P<0.05）；肠道肥大细胞数量各肠段组间无显著差异（P>0.05）。上述结果表明，早期补喂瘤胃液对羔羊体液免疫无显著影响，可在一定程度上增强由免疫细胞介导的免疫功能；早期补喂瘤胃液对羔羊小肠转运母源免疫球蛋白无显著影响；早期补喂瘤胃液能刺激羔羊小肠黏膜免疫系统的发育，使免疫屏障功能尽早完善。

关键词： 新生羔羊; 瘤胃液; 血浆; 免疫球蛋白; 细胞因子; 黏膜免疫相关细胞

本文引用格式

吴婷婷 , 韩冰 , 刘振 , 何周瑞 , 杨开伦 . 早期补喂瘤胃液对羔羊血浆免疫球蛋白、细胞因子和小肠黏膜免疫相关细胞的影响[J]. 动物营养学报, 2016 , 28(12) : 3943 -3951 . DOI: 10.3969/j.issn.1006-267x.2016.12.028

Abstract

This experiment aimed to study the effects of early administration of ruminal fluid on plasma immunoglobins and cytokines, and mucosal immunity-associated cells in small intestine in lambs. Twelve Texel newborn lambs were randomly allotted to two groups with 6 lambs per group. Ruminal fluid was orally administered once daily over a period of 14 days in trial group, and physiological saline was administered in control group. Blood was collected from jugular vein to separate plasma at 7, 14, 21 and 28 days of age, respectively; lambs were slaughtered to obtain small intestinal digesta at 28 days of age. Immunoglobins[immunoglobin A (IgA) and immunoglobin G (IgG)] and cytokines[interleukin-1β (IL-1β), interleukin-6(IL-6), tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ)] contents in plasma, and IgG and immunoglobin Fc fragment (Fc) contents in intestinal digesta were determined. The results showed as follows:at 21 days of age, the contents of IgA and IgG in plasma in trial group was significantly lower than those in control group (P<0.05 or P<0.01), but there were no significant differences at the other days of ages (P>0.05); the contents of IL-1β and IL-6 in plasma in trial group were higher than those in control group, but the differences were not significant (P>0.05); plasma TNF-α content in trial group was significantly higher than that in control group at 14 days of age (P<0.05), but there were no significant differences at the other days of ages (P>0.05); plasma IFN-γ content in trial group was higher than that in control group, but the difference was not significant (P>0.05); there was no significant difference of IgG and Fc contents in intestinal digesta at 28 days of age (P>0.05); the administration of ruminal fluid increased intestinal intraepithelial lymphocyte count, especially in ileum, trial group was significantly different from control group (P<0.05); mast cell count at different intestinal segments was not significantly different between groups (P>0.05). It is concluded that early administration of ruminal fluid has no influence on humoral immune in lambs, while to some extent, can enhance cell-mediated immune; early administration of ruminal fluid has no influence on intestinal transport of maternal immunoglobins in lambs; early administration of ruminal fluid can stimulate intestinal mucosal immune system in lambs, and contribute to immunologic barrier establishment.

Key words： newborn lamb; ruminal fluid; plasma; immunoglobin; cytokine; mucosal immunity-associated cell

参考文献

[1] POUNDEN W D,HIBBS J W.The influence of pasture and rumen inoculation on the establishment of certain microorganisms in the rumen of young dairy calves[J].Journal of Dairy Science,1949,32(12):1025-1031.

[2] JAMES R E,POLAN C E,BIBB T L,et al.Effect of orally administered duodenal fluid on susceptibility of newborn calves to an Escherichia coli challenge[J].Journal of Dairy Science,1976,59(8):1495-1501.

[3] MUSCATO T V,TEDESCHI L O,RUSSELL J B.The effect of ruminal fluid preparations on the growth and health of newborn,milk-fed dairy calves[J].Journal of Dairy Science,2002,85(3):648-656.

[4] ZHONG R Z,SUN H X,LI G D,et al.Effects of inoculation with rumen fluid on nutrient digestibility,growth performance and rumen fermentation of early weaned lambs[J].Livestock Science,2014,162:154-158.

[5] COSTERTON J W,DAMGAARD H N,CHENG K J.Cell envelope morphology of rumen bacteria[J].Journal of Bacteriology,1974,118(3):1132-1143.

[6] KRAUSE D O,RESSELL J B.How many ruminal bacteria are there?[J].Journal of Dairy Science,1996,79(8):1467-1475.

[7] BRANDTZAEG P,TOLO K.Mucosal penetrability enhanced by serum-derived antibodies[J].Nature,1977,266(5599):262-263.

[8] LOGAN E F,MCBEATH D G,LOWMAN B G.Quantitative studies on serum immunoglobulin levels in suckled calves from birth to five weeks[J].Veterinary Record,1974,94(16):367-370.

[9] 吴婷婷.补喂4种方法处理的瘤胃液制备物对新生羔羊肠道黏膜免疫的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2013:35-36.

[10] 张俐华.补喂绵羊瘤胃液制备物对1-35日龄羔羊、犊牛增重和血浆免疫球蛋白含量的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2013:30.

[11] SCHAREK L,GUTH J,REITER K,et al.Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets[J].Veterinary Immunology and Immunopathology,2005,105(1/2):151-161.

[12] SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of preweaning calves[J].Journal of Dairy Science,2010,93(12):5851-5855.

[13] BURTON J L,KENNEDY B W,BURNSIDE E B,et al.Variation in serum concentrations of immunoglobulins G,A,and M in Canadian Holstein-Friesian calves[J].Journal of Dairy Science,1989,72(1):135-149.

[14] ASHIDA H,OGAWA M,KIM M,et al.Bacteria and host interactions in the gut epithelial barrier[J].Nature Chemical Biology,2011,8(1):36-45.

[15] BAUER E,WILLIAMS B A,SMIDT H,et al.Influence of the gastrointestinal microbiota on development of the immune system in young animals[J].Currect Issues Intestinal Microbiol,2006,7(2):35-52.

[16] KLAENHAMMER T R,KLEEREBEZEM M,KOPP M V,et al.The impact of probiotics and prebiotics on the immune system[J].Nature Reviews Immunology,2012,12(10):728-734.

[17] AKDIS M,BURGLER S,CRAMERI R,et al.Interleukins,from 1 to 37,and interferon-γ:receptors,functions,and roles in diseases[J].The Journal of Allergy and Clinical Immunology,2011,128(4):739.

[18] SOBOTA R M,MVLLER P J,KHOURI C,et al.SHPS-1/SIRP1α contributes to interleukin-6 signalling[J].Cellular Signaling,2008,20(7):1385-1391.

[19] LIBBY P,RIDKER P M,MASERI A.Inflammation and atherosclerosis[J].Circulation,2002,105(9):1135-1143.

[20] GESSANI S,BELARDELLI F.IFN-γ expression in macrophages and its possible biological significance[J].Cytokine & Growth Factor Reviews,1998,9(2):117-123.

[21] FRUCHT D M,FUKAO T,BOGDAN C,et al.IFN-γ production by antigen-presenting cells:mechanisms emerge[J].Trends in Immunology,2001,22(10):556-660.

[22] MAASSEN C B M,VAN HOLTEN-NEELEN C,BALK F,et al.Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains[J].Vaccine,2000,18(23):2613-2623.

[23] CASTILLO N A,PERGIGÓN G,DE MORENO DE LEBLANC A.Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice[J].BMC Microbiology, 2011,11(1):177.

[24] MARTIN M G,WU S V,WALSH J H.Ontogenetic development and distribution of antibody transport and Fc receptor mRNA expression in rat intestine[J].Digestive Diseases and Sciences,1997,42(5):1062-1069.

[25] RODEWALD R.pH-dependent binding of immunoglobulins to intestinal cells of the neonatal rat[J].The Journal of Cell Biology,1976,71(2):666-669.

[26] GHETIE V,WARD E S.Multiple roles for the major histocompatibility complex class Ⅰ-related receptor FcRn[J].Annual Review of Immunology,2000,18(1):739-766.

[27] BENLOUNES N,CHEDID R,THUILLIER F,et al.Intestinal transport and processing of immunoglobulin G in the neonatal and adult rat[J].Biology of the Neonate,1995,67(4):254-263.

[28] ISRAEL E J,TAYLOR S,WU Z,et al.Expression of the neonatal Fc receptor,FcRn,on human intestinal epithelial cells[J].Immunology,1997,92(1):69-74.

[29] XU R J,WANG F,ZHANG S H.Postnatal adaptation of the gastrointestinal tract in neonatal pigs:a possible role of milk-borne growth factors[J].Livestock Production Science,2000,66(2):95-107.

[30] GASKINS H R.The intestinal immune system:gut reaction and growth of the pig[J].Journal of Animal Science,1996,74(Suppl.1):169.

[31] 陈付菊,陈耀星,王子旭,等.新生犊牛小肠黏膜结构的早期发育及上皮内淋巴细胞和杯状细胞的数量变化[J].中国兽医科学,2007,37(6):519-523.

[32] STENTON G R,VLIAGOFTIS H,BEFUS A D.Role of intestinal mast cells in modulating gastrointestinal pathophysiology[J].Annals of Allergy,Asthma & Immunology,1998,81(1):1-12,15.

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