不同钙、磷水平饲粮对12~15月龄梅花鹿生长性能、营养物质消化率及钙、磷代谢的影响

  • 孙伟丽 ,
  • 赵海平 ,
  • 王晓旭 ,
  • 樊燕燕 ,
  • 鲍坤 ,
  • 王艳梅 ,
  • 李光玉
展开
  • 1. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学国家重点实验室, 长春 130112;
    2. 长春科技学院, 长春 130600

收稿日期: 2016-05-16

  网络出版日期: 2016-12-17

基金资助

吉林省重大科技攻关专项(20140203018NY);吉林省科技发展计划项目(20150204071NY)

Effects of Diets with Different Calcium and Phosphorus Levels on Growth Performance, Nutrient Digestibility, Calcium and Phosphorus Metabolism of 12 to 15 Months Old Sika Deer

  • SUN Weili ,
  • ZHAO Haiping ,
  • WANG Xiaoxu ,
  • FAN Yanyan ,
  • BAO Kun ,
  • WANG Yanmei ,
  • LI Guangyu
Expand
  • 1. Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun 130112, China;
    2. Changchun Sci-Tech University, Changchun 130600, China

Received date: 2016-05-16

  Online published: 2016-12-17

摘要

本试验旨在通过研究不同钙、磷水平饲粮对12~15月龄梅花鹿生长性能、营养物质消化率及钙、磷代谢的影响,确定该时期适宜的饲粮钙、磷水平。选取16头健康、遗传特性相近的12月龄雄性梅花鹿,随机分为4组(每组4个重复,每个重复1头),组间体重无显著差异(P>0.05)。4组饲粮钙、磷水平分别为0.49%、0.33%(Ⅰ组),0.82%、0.56%(Ⅱ组),1.16%、0.80%(Ⅲ组)和1.61%、1.12%(Ⅳ组)。试验从2015年5月15日开始,至2015年8月7日结束。结果表明:1)各组12~15月龄梅花鹿各时期体重、平均日增重、平均日采食量和料重比的差异均不显著(P>0.05)。从整个试验期看,以Ⅳ组的生长性能较优。2)除第30天时Ⅲ组血清钙水平显著低于Ⅰ组和Ⅳ组(P<0.05)外,其他时间点(第1天、第60天、第83天)各组之间差异不显著(P>0.05)。Ⅰ组、Ⅱ组、Ⅲ组血清钙水平随试验时间的延长均没有产生显著变化(P>0.05),而Ⅳ组则随试验时间的延长呈现逐渐升高趋势,在第83天时表现最高,为2.415 nmol/L,显著高于第1天的2.280 nmol/L(P<0.05)。血清磷水平在同一时间点不同组别之间无显著差异(P>0.05),同一组不同时间点之间也没有显著差异(P>0.05)。试验第1天,Ⅰ组血清镁水平显著高于Ⅲ组和Ⅳ组(P<0.05);试验第30天,Ⅰ组血清镁水平显著高于Ⅲ组(P<0.05)。随试验时间的延长,Ⅰ组、Ⅱ组和Ⅲ组血清镁水平无显著变化(P>0.05),但Ⅳ组血清镁水平则表现为先升高后降低的趋势,第30天和第60天时显著高于第1天和第83天时(P<0.05)。3)各组间血清睾酮和雌二醇水平均无显著变化(P>0.05);血清甲状旁腺素、骨钙素水平及碱性磷酸酶活性随着饲粮钙、磷水平的升高无显著变化(P>0.05)。4)从整个试验期看,12~15月龄梅花鹿粗蛋白质和粗脂肪消化率以Ⅲ组最高,钙、磷消化率也以Ⅲ组最高,Ⅲ组钙消化率达到51.08%、磷消化率达到67.71%。由此得出,饲粮钙、磷水平对12~15月龄梅花鹿血清性激素及钙、磷、镁水平无显著影响,但营养物质消化率以Ⅲ组最高,因此推荐此时期饲粮钙、磷水平分别为1.16%和0.80%。

本文引用格式

孙伟丽 , 赵海平 , 王晓旭 , 樊燕燕 , 鲍坤 , 王艳梅 , 李光玉 . 不同钙、磷水平饲粮对12~15月龄梅花鹿生长性能、营养物质消化率及钙、磷代谢的影响[J]. 动物营养学报, 2016 , 28(12) : 3952 -3961 . DOI: 10.3969/j.issn.1006-267x.2016.12.029

Abstract

This experiment aimed to study the effects of diets with different calcium and phosphorus levels on growth performance, nutrient digestibility, calcium and phosphorus metabolism of 12 to 15 months old sika deer, in order to confirm the appropriate calcium and phosphorus levels for 12 to 15 months old sika deer. Sixteen healthy male sika deer of similar hereditary were randomly divided into four groups, and the difference in body weight was not significant among groups (P>0.05). Each group contained four replicates and each replicate had one deer. The levels of calcium and phosphorus in the four groups were 0.49% and 0.33% (group Ⅰ), 0.82% and 0.56% (group Ⅱ), 1.16% and 0.80% (group Ⅲ), 1.61% and 1.12% (group Ⅳ), respectively. The experimental period was from May 15th, 2015 to August 7th, 2015. The results showed as follows:1) there were no significant differences in body weight, average daily gain (ADG), average daily feed intake (ADFI) and feed weight ratio (F/G) of 12 to 15 months old sika deer in different time among four groups (P>0.05). From the whole experimental period, the growth performance of group Ⅳ was better than other three groups. 2) Serum calcium level in group Ⅲ was significantly lower than that in groups Ⅰ and Ⅳ on the 30th day (P<0.05), but there was no significant difference among four groups on the other time points (the 1st day, the 60th day and the 83rd day) (P>0.05). As the extension of experimental time, serum calcium level in groups Ⅰ, Ⅱ and Ⅲ had no significant change (P>0.05), but the group Ⅳ showed a gradually upward trend, and obtained the highest value of 2.415 nmol/L on the 83rd day which significantly higher than the value of 2.280 nmol/L on the 1st day (P<0.05). There was no significant difference in serum phosphorus level among different groups on the same time point (P>0.05), and the serum phosphorus level in each group had no significant change on different time points (P>0.05). Serum magnesium level in group Ⅰ was significantly higher than that in groups Ⅲ and Ⅳ on the 1st day (P<0.05), and significantly higher than that in group Ⅲ on the 30th day (P<0.05). As the extension of experimental time, serum magnesium level in groups Ⅰ, Ⅱ and Ⅲ had no significant change (P>0.05), but the group Ⅳ showed a trend of first increasing and then decreasing and it on the 30th day and the 60th day was significantly higher than that on the 1st day and the 83rd day (P<0.05).3) Serum testosterone and estradiol levels showed no significant changes in each group (P>0.05), and serum parathyroid hormone, osteocalcin levels and alkaline phosphatase activity also showed no significant changes with the calcium and phosphorus levels increasing (P>0.05).4) During the whole experimental period, the digestibility of crude protein, crude fat, calcium and phosphorus in the group Ⅲ was the highest, and the digestibility of calcium and phosphorus was 51.08% and 67.71%, respectively. In conclusion, dietary calcium and phosphate levels have no significant effects on the serum sex hormone, calcium, magnesium and phosphate levels of 12 to 15 months old sika deer, but the nutrient digestibility is the highest in group Ⅲ, so it is recommended that diet with 1.16% calcium and 0.80% phosphorus is suitable for 12 to 15 months old sika deer.

参考文献

[1] 李春义,赵世臻,王文英.鹿茸[M].北京:中国农业科技出版社,1988.

[2] CHAPMAN D I.Antlers-bones of contention[J].Mammal Review,1975,5(4):121-172.  

[3] 付强,刘源.钙、磷与维生素D对动物骨代谢的影响研究进展[J].中国比较医学杂志,2006,16(8):502-505.

[4] 初汉平.奶牛钙磷适宜供给量的研究[D].硕士学位论文.泰安:山东农业大学,2005.

[5] MUIR P D,SYKES A R.Effect of winter nutrition on antler development in red deer (Cervus elaphus):a field study[J].New Zealand Journal of Agricultural Research,1988,31(2):145-150.  

[6] MUIR P D,李春义.[J].国外特种经济动植物,1988(1):32.

[7] MUIR P D,张春生,王光雷.冬季营养对红鹿鹿角发育的影响[J].国外畜牧学:草食家畜,1989(4):42-45.

[8] 王峰,金顺丹,高秀华,等.三岁梅花鹿生茸期日粮中适宜钙、磷含量水平的研究[J].动物营养学报,1997,9(1):35-38.

[9] 郜玉钢,高秀华,佟煜人,等.日粮钙水平对6岁梅花鹿产茸性能的影响[J].中国畜牧杂志,2000,36(6):16-17.

[10] 郜玉钢,高秀华,佟煜人,等.6岁梅花鹿生茸期饲粮适宜钙水平的研究[J].动物营养学报,2000,12(3):48-51.

[11] 王钰明,赵峰,陈寿飞,等.猪生长阶段与饲粮类型对酸不溶灰分法测定养分消化率的影响[J].动物营养学报,2015,27(3):811-819.

[12] 沈同,王镜岩.生物化学[M].2版.北京:高等教育出版社,1998.

[13] LI C,SUTTIE J M.Histological examination of the antlerogenic region of red deer (Cervus elaphus) hummels[J].New Zealand Veterinary Journal,1996,44(4):126-130.  

[14] BARTOS L,BUBENIK G A,KUZMOVA E.Endocrine relationships between rank-related behavior and antler growth in deer[J].Frontiers in Bioscience,2012,4:1111-1126.

[15] LI C Y,LITTLEJOHN R P,SUTTIE J M.Effects of insulin-like growth factor 1 and testosterone on the proliferation of antlerogenic cells in vitro[J].Journal of Experimental Zoology,1999,284(1):82-90.  

[16] SUTTIE J M,LINCOLN G A,KAY R N B,et al.Endocrine control of antler growth in red deer stags[J].The Journal of the Society for Reproduction andFertility,1984,71(1):7-15.  

[17] SUTTIE J M,FENNESSY P F,LAPWOOD K R,et al.Role of steroids in antler growth of red deer stags[J].Journal of Experiment Zoology,1995,271(2):120-130.  

[18] FENNESSY P F,SUTTIE J M.Antler growth:nutritional and endocrine factors[M]//FENNESSY P F,DREW K R.Biology of deer production.New Zealand:Royal Society of New Zealand,1985,22:239-250.

[19] SUTTIE J M,KAY R N B.The influence of nutrition and photoperiod on the growth antlers of young red deer[M]//BROWN R D.Antler development in cervidae.Kingsville,Texas:Wildlife Research Institute,1982:61-71.

[20] 高志光,李春义,刘钟安,等.梅花鹿鹿茸生长速度、骨化程度与睾酮、雌二醇、碱性磷酸酶关系的研究[J].畜牧兽医学报,1988,19(3):171-176.

[21] BANKS W J,Jr,EPLING G P,KAINER R A,et al.Antler growth and osteoporosis Ⅰ.Morphological and morphometric changes in the costal compacta during the antler growth cycle[J].The Anatomical Record:Advances in Integrative Anatomy and Evolutionary Biology,1968,162(4):387-397.

[22] MOEN R,PASTOR J.Simulating antler growth and energy,nitrogen,calcium and phosphorus metabolism in caribou[J].The Seventh North American Caribou Conference,1996(10):85-97.

[23] GRASMAN B T,HELLGREN E C.Phosophorus nutrition in white-tailed deer:nutrient balance,physiological responses,and antler growth[J].Ecology,1993,74(8):2279-2296.  
文章导航

/