豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响

赵娜; 杨雪海; 陈芳; 郭万正; 黄少文; 张巍; 李绍章; 严念东; 魏金涛

doi:10.3969/j.issn.1006-267x.2016.12.030

动物营养学报 >

2016 , Vol. 28 >Issue 12: 3962 - 3969

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.030

豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响

赵娜 ,
杨雪海 ,
陈芳 ,
郭万正 ,
黄少文 ,
张巍 ,
李绍章 ,
严念东 ,
魏金涛

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1. 湖北省农业科学院畜牧兽医研究所, 武汉 430064;
2. 动物胚胎工程及分子育种湖北省重点实验室, 武汉 430064

收稿日期: 2016-07-01

网络出版日期: 2016-12-17

基金资助

湖北省农业科技创新团队项目（2016-620-000-001-028）；湖北省农业科学院扶持项目（2014fcxjh09）

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Effects of Soybean Enzymatic Fermentation Product on Growth Performance, Serum Indices and Intestinal Microflora Structure of Piglets

ZHAO Na ,
YANG Xuehai ,
CHEN Fang ,
GUO Wanzheng ,
HUANG Shaowen ,
ZHANG Wei ,
LI Shaozhang ,
YAN Niandong ,
WEI Jintao

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1. Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;
2. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Wuhan 430064, China

Received date: 2016-07-01

Online published: 2016-12-17

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摘要

本试验旨在研究豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响。选用144头49日龄健康的“杜×长×大”三元杂交育肥仔猪，随机分为3组，每组6个重复，每个重复8头猪（公母各占1/2）。阴性对照组饲喂无抗生素添加的基础饲粮，抗生素对照组饲喂基础饲粮+60 g/t吉他霉素，试验组饲喂试验饲粮（基础饲粮中10%豆粕被豆粕酶解发酵物替代）。试验期为42 d。结果表明：1）试验组仔猪的末重、平均日增重、平均日采食量分别比阴性对照组高5.06%、10.07%、5.03%（P<0.05），腹泻率比阴性对照组低39.59%（P<0.05），与抗生素对照组相比各指标差异均不显著（P>0.05）。2）试验组仔猪血清碱性磷酸酶和谷丙转氨酶活性、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）活性均显著高于阴性对照组（P<0.05），丙二醛（MDA）含量显著低于阴性对照组（P<0.05）；试验组和抗生素对照组仔猪血清指标差异不显著（P>0.05）。3）试验组仔猪盲肠、大肠中乳酸杆菌数量显著高于阴性对照组和抗生素对照组（P<0.05），十二指肠中总细菌数显著低于阴性对照组和抗生素对照组（P<0.05）。综上，豆粕酶解发酵物能提高仔猪生长性能，增强机体抗氧化能力，利于乳酸杆菌在肠道内形成优势菌群，降低腹泻率，生长性能达到使用抗生素水平。

关键词： 豆粕; 酶解发酵; 仔猪; 抗氧化能力; 肠道菌群

本文引用格式

赵娜 , 杨雪海 , 陈芳 , 郭万正 , 黄少文 , 张巍 , 李绍章 , 严念东 , 魏金涛 . 豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响[J]. 动物营养学报, 2016 , 28(12) : 3962 -3969 . DOI: 10.3969/j.issn.1006-267x.2016.12.030

Abstract

This study was designed to study the effects of soybean enzymatic fermentation product on growth performance, serum indices and intestinal microflora structure of piglets. One hundred and forty-four 49-day-old Duroc×Landrace×Yorkshire crossbred fattening piglets were randomly divided into three groups with six replicates and eight pigs in each replicate (male and female each half). Pigs in negative control (NC) group were fed a basal diet without adding antibiotics, in antibiotics control (AC) group were fed the basal diet supplemented with 60 g/t kitasamycin, and in test group were fed the test diet (10% soybean meal was replaced by enzymatic fermentation product in basal diet). The results showed as follows:1) the final weight, average daily gain and average daily feed intake of pigs in test group were 5.06%, 10.07% and 5.03% higher while diarrhea rate was 39.59% lower than those in NC group (P<0.05), but no significant different with AC group (P>0.05). 2) Serum activities of alkaline phosphatase and alanine aminotransferase, total antioxidant capacity (T-AOC), and superoxide dismutase (SOD) activity of pigs in test group were significantly higher (P<0.05), but serum malondialdehyde (MDA) content was significantly lower than those in NC group (P<0.05). Notably, there was no significant difference in serum indices between AC group and test group (P>0.05). 3) Compared with NC and AC groups, test group significantly increased the number of Lactobacillus in cecum and large intestine (P<0.05), but significantly decreased the number of total bacteria in duodenum of pigs (P<0.05). In conclusion, soybean enzymatic fermentation product can improve the growth performance and body's antioxidant capacity of piglets, has beneficial effect on Lactobacillus colonization in gut, and makes the performance reached the antibiotic using level.

Key words： soybean; enzymatic fermentation; piglets; antioxidant capacity; intestinal flora

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