动物营养学报 >

2016 , Vol. 28 >Issue 12: 4054 - 4062

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.041

饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响

  • 王贞杰 ,
  • 叶保民 ,
  • 常青 ,
  • 陈四清 ,
  • 刘长琳 ,
  • 胡建成 ,
  • 严俊丽 ,
  • 卢斌
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  • 1. 农业部海洋渔业资源可持续利用重点开放试验室, 中国水产科学研究院黄海水产研究所, 青岛 266071;
    2. 上海海洋大学水产与生命学院, 上海 201306;
    3. 辽宁省海洋与渔业厅, 沈阳 110000

收稿日期: 2016-06-16

  网络出版日期: 2016-12-17

基金资助

中国水产科学研究院黄海水产研究所基本科研业务费资助（20603022016005）

收起

Effects of Dietary Vitamin C Content on Anti-Ammonia-Nitrite Stress Ability of Juvenile Spotted Halibut (Verasper variegatus)

  • WANG Zhenjie ,
  • YE Baomin ,
  • CHANG Qing ,
  • CHEN Siqing ,
  • LIU Changlin ,
  • HU Jiancheng ,
  • YAN Junli ,
  • LU Bin
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  • 1. Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    2. College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
    3. Administration of Ocean and Fisheries of Liaoning Province, Shenyang 110000, China

Received date: 2016-06-16

  Online published: 2016-12-17

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摘要

氨氮污染是水产养殖中重要的污染物，因此提高鱼体抗污染应激能力至关重要。本试验采用不同维生素C含量的饲料饲喂圆斑星鲽幼鱼，探究饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响。试验在（12.5±1.5）℃的水温下进行，选择体重为（38.0±0.8）g的健康圆斑星鲽幼鱼，随机分为7组（每组3个重复，每个重复30尾），分别投喂维生素C含量为10.2（对照）、249.1、402.8、616.2、769.5、909.4和1 177.8 mg/kg的试验饲料8周。投喂试验结束后，从每个重复取10尾鱼，用20 mg/L的氨氮胁迫24 h。结果表明：无论是氨氮胁迫前还是氨氮胁迫后，在饲料维生素C含量达到769.5 mg/kg时，肝脏和肌肉中维生素C积累达到饱和，再继续升高饲料维生素C含量，肝脏、肌肉中维生素C含量不再显著升高（P>0.05）。除1 177.8 mg/kg维生素C组外，无论是氨氮胁迫前还是氨氮胁迫后，各维生素C添加组圆斑星鲽幼鱼血清中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性均显著高于对照组（P<0.05）。除616.2 mg/kg维生素C组血清中CAT活性无显著变化（P>0.05）外，氨氮胁迫使各组血清中CAT和SOD活性显著降低（P<0.05），但添加维生素C可以减少降低的幅度。无论是氨氮胁迫前还是氨氮胁迫后，各维生素C添加组鳃丝Na+/K+-ATP酶活性均显著高于对照组（P<0.05）。氨氮胁迫使对照组及909.4和1 177.8 mg/kg维生素C组鳃丝Na+/K+-ATP酶活性显著降低（P<0.05），其他组则无显著变化（P>0.05）。氨氮胁迫使各组血清中葡萄糖和乳酸含量显著升高（P<0.05），对照组血清中皮质醇含量显著升高（P<0.05）。此外，氨氮胁迫使各组血清中总铁结合力显著降低（P<0.05）。综合各项测定指标，圆斑星鲽幼鱼饲料中维生素C含量在402.8~616.2 mg/kg时可有效提高机体抗氨氮胁迫能力。

关键词： 圆斑星鲽幼鱼; 维生素C; 应激反应; 抗氨氮胁迫

本文引用格式

王贞杰 , 叶保民 , 常青 , 陈四清 , 刘长琳 , 胡建成 , 严俊丽 , 卢斌 . 饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响[J]. 动物营养学报, 2016 , 28(12) : 4054 -4062 . DOI: 10.3969/j.issn.1006-267x.2016.12.041

Abstract

Ammonia-nitrogen pollution is one of the important pollutants in aquaculture, thus improving the resistance of pollution stress of the fish is very important. This experiment using different vitamin C content diets to feed juvenile spotted halibut (Verasper variegatus), in order to get the effects of dietary vitamin C content on anti-ammonia-nitrogen stress ability of juvenile spotted halibut. Water temperature was maintained at (12.5±1.5)℃ in this experiment. Juvenile spotted halibut with the body weight of (38.0±0.8) g were randomly assigned into 7 groups with 3 replicates per group and 30 fish per replicate, and fed 7 experimental diets with the vitamin C content was 10.2, 249.1, 402.8, 616.2, 769.5, 909.4 and 1 177.8 mg/kg, respectively. The experiment lasted for 8 weeks. After feeding trial, each replicate selected 10 fish to stress 24 h by 20 mg/L ammonia-nitrogen. The results showed as follows:whether before or after ammonia-nitrite stress, the vitamin C accumulation in muscle and liver reached saturation when dietary vitamin C content was 769.5 mg/kg, and continued to rise the dietary vitamin C content, the vitamin C content in muscle and liver was not significantly increased (P>0.05). The activities of serum catalase (CAT) and superoxide dismutase (SOD) in vitamin C addition groups except 1 177.8 mg/kg vitamin C group were significantly higher than those in control group whether before or after ammonia-nitrite stress (P<0.05). The activities of serum CAT and SOD in groups except serum CAT activity in 616.2 mg/kg vitamin C group were significantly decreased by ammonia-nitrite stress (P<0.05), and decline ranges in vitamin C addition groups were less compared with control group. The gill Na+/K+-ATPase activity in vitamin C addition groups was significantly higher than that in control group whether before or after ammonia-nitrite stress (P<0.05). The gill Na+/K+-ATPase activity in control group and 909.4, 1 177.8 mg/kg vitamin C groups was significantly decreased (P<0.05), but that in other groups had no significant change after ammonia-nitrite stress (P>0.05). Ammonia-nitrite stress significantly increased the contents of serum glucose and lactic acid in all groups (P<0.05), and significantly increased the serum cortisol content in control group (P<0.05), while significantly decreased the serum total iron binding capacity (TIBC) in all groups (P<0.05). Base on a comprehensive consideration of various measured indices, when dietary vitamin C content is 402.8 to 616.2 mg/kg, the anti-ammonia-nitrogen stress ability of juvenile spotted halibut can be effectively improved.

Key words： juvenile spotted halibut (Verasper variegatus); vitamin C; stress response; anti-ammonia-nitrite stress

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