动物营养学报 >

2017 , Vol. 29 >Issue 4: 1205 - 1217

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.04.016

实验方法 Experimental Methods

体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱

展开
  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 中国农业科学院淮安健康养殖与饲料研究中心, 淮安 223005;
    3. 江苏华威农牧发展有限公司, 淮安 223005
高理想(1987-),男,安徽阜阳人,博士研究生,从事饲料生物学效价评定研究。E-mail:glxahu@163.com

收稿日期: 2016-09-02

  网络出版日期: 2017-04-14

基金资助

国家科技支撑计划项目课题（2012BAD39B01）；中国农业科学院科技创新工程（ASTIP-IAS07）；产学研联合创新资金（BY2013054）；苏北科技发展计划（BN2014034）

收起

Optimization of Non-Starch Polysaccharide Enzymes of Diets for Growing Pigs Using in Vitro Method

Expand
  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agriculture Science, Beijing 100193, China;
    2. Huai'an Research Center of Healthy Breeding and Feed Science, Chinese Academy of Agriculture Science, Huaian 223005, China;
    3. Jiangsu Huawei Animal Feed Development Co. Ltd., Huai'an 223005, China

Received date: 2016-09-02

  Online published: 2017-04-14

Fold

摘要

针对饲粮非淀粉多糖（NSP）酶的海量筛选工作和动物试验间的可比性差等问题，本研究探讨使用体外模拟法优化生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的NSP酶谱。首先采用单因素随机试验设计，研究NSP酶的添加水平与饲粮体外干物质消化率（IVDMD）的关系。在玉米-豆粕型饲粮和玉米-杂粕型饲粮中分别添加不同水平的纤维素酶、木聚糖酶、β-葡聚糖酶、β-甘露聚糖酶、α-半乳糖苷酶和果胶酶6种NSP酶，分析各NSP酶对饲粮IVDMD的作用效果。然后采用二次回归旋转正交组合试验设计，筛选2种饲粮中6种NSP酶的最佳酶谱。结果表明：1）6种NSP酶的添加水平与2种类型猪饲粮IVDMD之间存在二次曲线关系。2）α-半乳糖苷酶对玉米-豆粕型饲粮的IVDMD提升最高，达到了1.28%，木聚糖酶对玉米-杂粕型饲粮的IVDMD提升最高，达到了1.95%。3）玉米-豆粕型饲粮的最佳酶谱为：纤维素酶533.6 U/kg、木聚糖酶9 983.7 U/kg、β-葡聚糖酶1 014.4 U/kg、β-甘露聚糖酶4 080.6 U/kg、α-半乳糖苷酶251.6 U/kg和果胶酶107.3 U/kg。玉米-杂粕型饲粮的最佳酶谱为：纤维素酶960.0 U/kg、木聚糖酶17 177.6 U/kg、β-葡聚糖酶405.8 U/kg、β-甘露聚糖酶19 023.2 U/kg、α-半乳糖苷酶307.2 U/kg和果胶酶96.9 U/kg。4）优化后的酶谱使玉米-豆粕型饲粮的IVDMD提升了3.26%，使玉米-杂粕型饲粮的IVDMD提升了3.75%。由此可见，6种NSP酶联合使用能够更大程度地提高生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的IVDMD。

关键词： 非淀粉多糖酶; 生长猪; 饲粮; 体外干物质消化率; 优化

本文引用格式

高理想, 陈亮, 崔世贵, 谢月华, 张宏福 . 体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱[J]. 动物营养学报, 2017 , 29(4) : 1205 -1217 . DOI: 10.3969/j.issn.1006-267x.2017.04.016

Abstract

Aimed to resolve the problem of massive work and poor comparability for screening non-starch polysaccharides (NSP) enzymes by animal experiment, the study was conducted to optimize the NSP enzymes combination of corn-soybean meal diet and corn-miscellaneous meal diet for growing pigs using an in vitro method. The first step was to determine the dose-response of the NSP enzymes supplemental level on the in vitro dry matter digestibility (IVDMD) of diets using one-way randomized experimental design. The NSP enzymes including cellulase, xylanase, β-glucanase, β-mannanase, α-galactosidase and pectinase were added into corn-soybean meal diet and corn-miscellaneous meal diet, and to analysis the effects of NSP enzymes on the IVDMD of diets. The second step was to screen the optimum enzyme combinations of 6 kinds NSP enzymes using quadratic orthogonal rotation combination design. The results showed as follows:1) quadratic effects of NSP enzymes on the IVDMD of diets for growing pig were observed. 2) The α-galactosidase had a greatest effect in all NSP enzymes of corn-soybean meal diet and increased the IVDMD by 1.28%; whereas xylanase increased the IVDMD of corn-miscellaneous meal diet by 1.95%. 3) The optimum enzyme combination in the corn-soybean meal diet was cellulase 533.6 U/kg, xylanase 9 983.7 U/kg, β-glucanase 1 014.4 U/kg, β-mannanase 4 080.6 U/kg, α-galactosidase 251.6 U/kg and pectinase 107.3 U/kg. The optimum enzyme combination in the corn-miscellaneous meal diet was cellulase 960.0 U/kg, xylanase 17 177.6 U/kg, β-glucanase 405.8 U/kg, β-mannanase 19 023.2 U/kg, α-galactosidase 307.2 U/kg and pectinase 96.9 U/kg. 4) The IVDMD of corn-soybean meal diet was increased by 3.26% and the IVDMD of the corn-miscellaneous meal diet was also increased 3.75% using the optimum NSP enzymes combination. In conclusion, the combination of the 6 NSP enzymes can improve the IVDMD of corn-soybean meal diet and corn-miscellaneous meal diet for growing pig diets more effectively.

Key words： NSP enzyme; pig; diet; in vitro dry matter digestibility; optimization

参考文献

[1] BACH KNUDSEN K E.Carbohydrate and lignin contents of plant materials used in animal feeding[J].Animal Feed Science and Technology,1997,67(4):319-338.  

[2] 黄庆华,陈亮,高理想,等.乙酸酐衍生化气相色谱法测定饲料非淀粉多糖含量时适宜称样量确定依据的研究[J].动物营养学报,2015,27(5):1620-1631.

[3] HOODA S,METZLER-ZEBELI B U,VASANTHAN T,et al.Effects of viscosity and fermentability of purified non-starch polysaccharides on ileal and total tract nutrient digestibility in ileal-cannulated grower pigs[J].Livestock Science,2010,134(1/2/3):79-81.

[4] CHOCT M,DERSJANT-LI Y,MCLEISH J,et al.Soy oligosaccharides and soluble non-starch polysaccharides:a review of digestion,nutritive and anti-nutritive effects in pigs and poultry[J].Asian-Australasian Journal of Animal Science,2010,23(10):1386-1398.  

[5] BROWNLEE I A.The physiological roles of dietary fibre[J].Food Hydrocolloids,2011,25(2):238-250.  

[6] NORTEY T N,PATIENCE J F,SIMMINS P H,et al.Effects of individual or combined xylanase and phytase supplementation on energy,amino acid,and phosphorus digestibility and growth performance of grower pigs fed wheat-based diets containing wheat millrun[J].Journal of Animal Science,2007,85(6):1432-1443.  

[7] ZHANG G G,YANG Z B,WANG Y,et al.Effects of dietary supplementation of multi-enzyme on growth performance,nutrient digestibility,small intestinal digestive enzyme activities,and large intestinal selected microbiota in weanling pigs[J].Journal of Animal Science,2014,92(5):2063-2069.  

[8] HE J,YIN J,WANG L,et al.Functional characterisation of a recombinant xylanase from Pichia pastoris and effect of the enzyme on nutrient digestibility in weaned pigs[J].British Journal of Nutrition,2010,103(10):1507-1513.  

[9] KIARIE E,NYACHOTI C M,SLOMINSKI B A,et al.Growth performance,gastrointestinal microbial activity,and nutrient digestibility in early-weaned pigs fed diets containing flaxseed and carbohydrase enzyme[J].Journal of Animal Science,2007,85(11):2982-2993.  

[10] OLUKOSI O A,SANDS J S,ADEOLA O.Supplementation of carbohydrases or phytase individually or in combination to diets for weanling and growing-finishing pigs[J].Journal of Animal Science,2007,85(7):1702-1711.  

[11] WOYENGO T A,SANDS J S,GUENTER W,et al.Nutrient digestibility and performance responses of growing pigs fed phytase-and xylanase-supplemented wheat-based diets[J].Journal of Animal Science,2008,86(4):848-857.  

[12] NITRAYOVÁ S,HEGER J,PATRÁŠP,et al.Effect of xylanase on apparent ileal and total tract digestibility of nutrients and energy of rye in young pigs[J].Archives of Animal Nutrition,2009,63(4):281-291.  

[13] ZIJLSTRA R T,OWUSU-ASIEDU A,SIMMINS P H.Future of NSP-degrading enzymes to improve nutrient utilization of co-products and gut health in pigs[J].Livestock Science,2010,134(1/2/3):255-257.

[14] BEDFORD M R,CLASSEN H L.An in vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes[J].Poultry Science,1993,72(1):137-143.  

[15] MALATHI V,DEVEGOWDA G.In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes[J].Poultry Science,2001,80(3):302-305.  

[16] 王恩玲,张宏福,赵峰,等.离体法优化肉鸭日粮非淀粉多糖酶酶谱的研究[J].饲料工业,2008,29(10):9-14.

[17] 何科林,萨仁娜,高杰,等.体外法优化肉鸡日粮非淀粉多糖酶[J].中国农业科学,2012,45(21):4457-4464.

[18] NARASIMHA J,NAGALAKSHMI D,REDDYY R,et al.Evaluation of non starch polysaccharide degrading enzymes and enzyme combinations for their ability to degrade non starch polysaccharides of layer diet by two stage in vitro digestion assay[J].Journal of Veterinary & Animal Sciences,2013,9(3):188-194.

[19] 胡光源,赵峰,张宏福,等.饲粮蛋白质来源与水平对生长猪空肠液组成的影响[J].动物营养学报,2010,22(5):1220-1225.

[20] CHEN L,GAO L X,HUANG Q H,et al.Prediction of digestible energy of feed ingredients for growing pigs using a computer-controlled simulated digestion system[J].Journal of Animal Science,2014,92(9):3887-3894.  

[21] 中华人民共和国农业部.NY/T 912-2004饲料添加剂纤维素酶活力的测定分光光度法[S].北京:中国农业出版社,2005.

[22] 中华人民共和国国家质量监督检验检疫总局.GB/T 23874-2009饲料添加剂木聚糖酶活力的测定分光光度法[S].北京:中国标准出版社,2009.

[23] 中华人民共和国农业部.NY/T 911-2004饲料添加剂β葡聚糖酶活力的测定分光光度法[S].北京:中国农业出版社,2005.

[24] NRC.Nutrient requirements of swine[S].11th ed.Washington,D.C.:National Academy Press,2012.

[25] 赵峰,张宏福,张子仪.单胃动物仿生消化系统操作手册[M].2版.北京:中国农业科学院,2011.

[26] BACH KNUDSEN K E,CANIBE N.Breakdown of plant carbohydrates in the digestive tract of pigs fed on wheat-or oat-based rolls[J].Journal of the Science of Food and Agriculture,2000,80(8):1253-1261.  

[27] BACH KNUDSEN K E,JØRGENSEN H.Impact of wheat and oat polysaccharides provided as rolls on the digestion and absorption processes in the small intestine of pigs[J].Journal of the Science of Food and Agriculture,2007,87(13):2399-2408.  

[28] VORAGEN A G J,COENEN G J,VERHOEF R P,et al.Pectin,a versatile polysaccharide present in plant cell walls[J].Structural Chemistry,2009,20(2):263-275.  

[29] OLUKOSI O A,BEDFORD M R,ADEOLA O.Xylanase in diets for growing pigs and broiler chicks[J].Canadian Journal of Animal Science,2007,87(2):227-235.  

[30] EMIOLA I A,OPAPEJU F O,SLOMINSKI B A,et al.Growth performance and nutrient digestibility in pigs fed wheat distillers dried grains with solubles-based diets supplemented with a multicarbohydrase enzyme[J].Journal of Animal Science,2009,87(7):2315-2322.  

[31] REILLY P,SWEENEY T,O'SHEA C,et al.The effect of cereal-derived beta-glucans and exogenous enzyme supplementation on intestinal microflora,nutrient digestibility,mineral metabolism and volatile fatty acid concentrations in finisher pigs[J].Animal Feed Science and Technology,2010,158(3/4):165-176.

[32] DIEBOLD G,MOSENTHIN R,PIEPHO H P,et al.Effect of supplementation of xylanase and phospholipase to a wheat-based diet for weanling pigs on nutrient digestibility and concentrations of microbial metabolites in ileal digesta and feces[J].Journal of Animal Science,2004,82(9):2647-2656.  

[33] SALEH F,OHTSUKA A,TANAKA T,et al.Effect of enzymes of microbial origin on in vitro digestibilities of dry matter and crude protein in soybean meal[J].Animal Science Journal,2003,74(1):23-29.  

[34] BOISEN S,FERNÁNDEZ J A.Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses[J].Animal Feed Science and Technology,1997,68(3/4):277-286.

[35] REGMI P R,SAUER W C,ZIJLSTRA R T.Prediction of in vivo apparent total tract energy digestibility of barley in grower pigs using an in vitro digestibility technique[J].Journal of Animal Science,2008,86(10):2619-2626.  

[36] SAUVANT D,PEREZ J M,TRAN G.Tables of composition and nutritional value of feed materials:pigs,poultry,cattle,sheep,goats,rabbits,horses and fish[M].Netherlands:Wageningen Academic Publishers,2004.

[37] CHOCT M.Feed non-starch polysaccharides:chemical structures and nutritional significance[J].Feed Milling International,1997,6:13-26.

[38] BENAMROUCHE S,CRÔNIER D,DEBEIRE P,et al.A chemical and histological study on the effect of (1→4)-β-endo-xylanase treatment on wheat bran[J].Journal of Cereal Science,2002,36(2):253-260.  

[39] KUMAR V,SINHA A K,MAKKAR H P S,et al.Dietary roles of non-starch polysachharides in human nutrition:a review[J].Critical Reviews in Food Science and Nutrition,2012,52(10):899-935.  

[40] KERR B J,SHURSON G C.Strategies to improve fiber utilization in swine[J].Journal of Animal Science and Biotechnology,2013,4:11.

[41] JUANPERE J,PÉREZ-VENDRELL A M,ANGULO E,et al.Assessment of potential interactions between phytase and glycosidase enzyme supplementation on nutrient digestibility in broilers[J].Poultry Science,2005,84(4):571-580.  

[42] 赵敏.非淀粉多糖酶对蛋鸡日粮能量代谢调控的研究[D].硕士学位论文.西宁:青海大学,2007.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2