动物营养学报 >

2017 , Vol. 29 >Issue 4: 1283 - 1293

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.04.025

饲料资源开发 Feed Resources Development

香蕉茎叶粉固态发酵条件优化及鹅对其养分利用率的研究

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  • 华南农业大学动物科学学院, 广州 510642
王增煌(1990-),男,福建松溪人,硕士研究生,从事非常规饲料资源研究。E-mail:397421559@qq.com

收稿日期: 2016-10-09

  网络出版日期: 2017-04-14

基金资助

公益性行业科研专项（201303143）

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Study on Optimal Solid State Fermentation Conditions of Banana Stems and Leaves Powder and Its Nutrient Utilization for Geese

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  • College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2016-10-09

  Online published: 2017-04-14

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摘要

本试验旨在研究应用固态发酵技术改善香蕉茎叶粉养分组成的工艺参数及鹅对其养分的利用率。选择米曲霉和产朊假丝酵母作为发酵菌种，逐步探究5个因素（单菌种发酵、硫酸铵添加量、米曲霉和产朊假丝酵母接种比例、混合菌液接种量）对发酵后香蕉茎叶粉蛋白质含量的影响。利用正交设计筛选最优发酵温度、底物水分和发酵时间的组合。结果显示：米曲霉和产朊假丝酵母单独发酵均能显著或极显著提高发酵后香蕉茎叶粉的粗蛋白质含量（P<0.05或P<0.01）。添加硫酸铵能显著或极显著提高发酵后香蕉茎叶粉的真蛋白质含量（P<0.05或P<0.01），其中添加2%硫酸铵组的真蛋白质含量最高。米曲霉∶产朊假丝酵母接种比例为2∶1的处理发酵后香蕉茎叶粉的蛋白质净增加量显著或极显著高于比例为1∶1、1∶3和3∶2的处理（P<0.05或P<0.01）。正交试验结果显示，以4%的接种量、接种比例为2∶1（米曲霉∶产朊假丝酵母）、2%的硫酸铵添加量，在基质水分为50%，30℃的环境下发酵4 d效果最佳。经过该工艺发酵后的香蕉茎叶粉粗蛋白质含量提高了33.82%，氨基酸分析结果显示，除赖氨酸和精氨酸外其余的15种氨基酸含量均有不同程度的提高。马冈鹅的代谢试验结果显示，发酵后香蕉茎叶粉中的粗蛋白质利用率提高了52.66%，极显著高于发酵前（P<0.01）；此外，代谢能和能量利用率也都略有提高（P>0.05）。由此可见，经过该发酵工艺发酵后的香蕉茎叶粉营养价值不仅得到了改善，也促进了鹅对其养分的消化吸收。

关键词： 香蕉茎叶粉; 固态发酵; 蛋白质; 养分利用率; 马冈鹅; 米曲霉; 产朊假丝酵母

本文引用格式

王增煌, 王文策, 翟双双, 谢强, 左鑫, 杨琳 . 香蕉茎叶粉固态发酵条件优化及鹅对其养分利用率的研究[J]. 动物营养学报, 2017 , 29(4) : 1283 -1293 . DOI: 10.3969/j.issn.1006-267x.2017.04.025

Abstract

This study was conducted to use the solid state fermentation technology to improve nutrient composition of banana stems and leaves and its nutrient utilization for geese. Choosing Aspergillus oryzae and Candida utilis as fermentation strains to investigate the influences of 5 factors[single strain fermentation, addition of (NH4)2SO4, proportion of Aspergillus oryzae and Candida utilis, mixed bacteria liquid inoculation] on the protein content of banana stems and leaves powder after fermentation. Using orthogonal design to find out the optimal combination of fermentation temperature, fermentation time and substrate moisture. The results showed that using Aspergillus oryzae or Candida utilis as single fermentation strain could significantly increase the crude protein content of fermented banana stems and leaves powder (P<0.05 or P<0.01). Fermentation with (NH4)2SO4 addition could significantly increase true protein content of fermented banana stems and leaves powder (P<0.05 or P<0.01), especially 2% addition. The net increase in protein treated with the proportion of Aspergillus oryzae and Candida utilis by 2:1 significantly higher than that of the proportion of 1:1, 1:3 and 3:2 (P<0.05 or P<0.01). The results of orthogonal test showed that the best fermentation condition were 4% inoculation (the proportion of Aspergillus oryzae and Candida utilis by 2:1) and 2% (NH4)2SO4 addition, and fermenting in 50% substrate moisture, 30℃ for 4 days. The crude protein content of banana stems and leaves increased by 33.82% after this fermentation process. The amino acid analysis showed that there were 15 kinds of amino acids increased differently beside lysine and arginine. The metabolic test results of Magang geese showed that the crude protein utilization of banana stems and leaves powder after fermentation significantly increased by 52.66% comparing with the banana stems and leaves powder without fermentation (P<0.01), additionally, the energy and metabolic energy utilization were increased slightly as well. In conclusion, the nutritional value of banana stems and leaves powder is improved after this fermentation process, and also promote the digestion and absorption of nutrient of geese.

Key words： banana stems and leaves powder; solid state fermentation; protein; nutrient utilization rate; Magang geese; Aspergillus oryzae; Candida utilis

参考文献

[1] 王增煌,王文策,杨琳.香蕉茎叶作为饲料原料的研究进展[J].中国畜牧杂志,2016,52(17):82-86.

[2] 李日强,张峰,张伟峰.氨化和固态发酵玉米秸秆生产饲料蛋白的研究[J].农业环境科学学报,2006,25(6):1636-1639.

[3] 莫重文.混合菌发酵豆渣生产蛋白质饲料的研究[J].中国饲料,2007(14):36-38.

[4] ROSMA A,CHEONG M W.Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium[J].Malaysian Journal of Microbiology,2007,3(1):19-26.

[5] 刘延琳,刘伟,夏加贵.白葡萄酒活性干酵母对不同氮源利用的研究[J].微生物学杂志,2007,27(1):88-90.

[6] 刘明启,刘光富,戴贤君,等.优化米曲霉固体发酵产果胶酶及产物酶学性质[J].中国计量学院学报,2010,21(2):64-69,178.

[7] 李平兰.食品微生物学教程[M].北京:中国林业出版社,2011.

[8] 籍保平,尤希风,张博润.苹果渣发酵生产饲料蛋白的培养基[J].中国农业大学学报,1999,4(6):53-56.

[9] 孙展英.马铃薯渣固态发酵工艺研究及生物学价值评定[D].硕士学位论文.保定:河北农业大学,2014.

[10] 欧荣娣,范觉鑫,王升平,等.红薯渣固态发酵条件优化[J].动物营养学报,2015,27(10):3302-3310.

[11] EL-GHONEMY D H,ALI T H,EL-BONDKLY A M,et al.Improvement of Aspergillus oryzae NRRL 3484 by mutagenesis and optimization of culture conditions in solid-state fermentation for the hyper-production of extracellular cellulase[J].Antonie van Leeuwenhoek,2014,106(5):853-864.  

[12] DOELLE H W,MITCHELL D A,ROLZ C E.Solid substrate cultivation[M].New York:Springer,1992.

[13] VU V H,PHAM T A,KIM K.Improvement of fungal strain by repeated and sequential mutagenesis and optimization of solid state fermentation for the hyper-production of raw-starch-digesting enzyme[J].Journal of Microbiology and Biotechnology,2010,20(4):718-726.  

[14] BHANJA T,ROUT S,BANERJEE R,et al.Comparative profiles of α-amylase production in conventional tray reactor and GROWTEK bioreactor[J].Bioprocess and Biosystems Engineering,2007,30(5):369-376.  

[15] MAURYA D P,SINGH D,PRATAP D,et al.Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei[J].Journal of Environmental Biology,2012,33(1):5-8.

[16] KIM D M,CHO E J,KIM J W,et al.Production of cellulases by Penicillium sp.in a solid-state fermentation of oil palm empty fruit bunch[J].African Journal of Biotechnology,2014,13(1):145-155.  

[17] 张冬艳,张瑞兰,张通.绿色木酶与产朊假丝酵母混合发酵生产纤维素酶和单细胞蛋白[J].内蒙古工业大学学报,2003,22(3):180-185.

[18] 吴远根,彭湘屏,张晓娟,等.产朊假丝酵母固态发酵麻疯树饼粕产菌体蛋白的研究[J].食品工业科技,2009(2):161-164.

[19] 陈中平.米曲霉发酵对豆粕营养特性的影响[D].硕士学位论文.雅安:四川农业大学,2010.

[20] 余冰,傅娅梅,叶楠,等.固态发酵对复合蛋白质饲料营养价值改善效果的研究[J].动物营养学报,2009,21(4):546-553.

[21] HONG K J,LEE C H,KIM S W.Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals[J].Journal of Medicinal Food,2004,7(4):430-435.  

[22] AHMED A E,SMITHARD R,ELLIS M.Activities of enzymes of the pancreas,and the lumen and mucosa of the small intestine in growing broiler cockerels fed on tannin-containing diets[J].British Journal of Nutrition,1991,65(2):189-197.  

[23] 张子仪.中国饲料学[M].北京:中国农业出版社,2000.

[24] ABDEL-NABY M A,EL-TANASH A B,SHERIEF A D A.Structural characterization,catalytic,kinetic and thermodynamic properties of Aspergillus oryzae tannase[J].International Journal of Biological Macromolecules,2016,92:803-811.

[25] PARANTHAMAN R,VIDYALAKSHMI R,SINGARAVADIVEL K.Comparative study on the suitability of different substrates for tannin acyl hydrolase production using Aspergillus oryzae[J].Journal of Pharmaceutical Sciences & Research,2009,1(4):36-42.

[26] PILLAI A R,DEVEGOWDA G,ANANDAKUMAR K.Influence of tannase and dried Aspergillus oryzae culture on performance of broilers fed tannin rich diets[J].Indian Journal of Animal Nutrition,1996,13(4):209-213.

[27] 牛建涛,张小英,官伟波.纤维素纤维织物在米曲霉作用下的生物降解[J].上海纺织科技,2013,41(5):1-3,19.
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