动物营养学报 >

2017 , Vol. 29 >Issue 5: 1527 - 1534

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.010

禽营养 Poultry nutrition

不同相对湿度对间歇性26℃环境下肉鸡盲肠菌群多样性的影响

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  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 河北工程大学农学院, 邯郸 056021
彭骞骞(1989-),女,河北邯郸人,硕士研究生,畜牧学专业。E-mail:18230221210@163.com

收稿日期: 2016-11-04

  网络出版日期: 2017-05-13

基金资助

国家科技支撑计划课题"畜禽健康养殖环境控制关键技术研究与集成"（2012BAD39B02）；中国农业科学院科技创新团队项目（ASTIP-IAS07）

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Effects of Different Relative Humidity on Cecal Microflora Diversity of Broilers under Intermittent 26℃ Environment

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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Agriculture, Hebei University of Engineering, Handan 056021, China

Received date: 2016-11-04

  Online published: 2017-05-13

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摘要

本试验旨在研究不同相对湿度（RH）对间歇性26℃环境下肉鸡盲肠菌群多样性的影响。选取29日龄爱拔益加（AA）肉鸡180只转入环境控制舱，随机分成3个组（RH分别为30%、60%和85%），每组6个重复，每个重复10只鸡（公母各5只）。从29日龄开始，每天10：00-16：00（6 h）温度维持26℃，RH分别为30%、60%和85%，剩余时间温度为21℃，RH为60%。试验共14 d。采用16S rDNA的变性梯度凝胶电泳（DGGE）技术，结合特异性和共性条带割胶回收DNA进行克隆和测序，分析RH在间歇性26℃偏热处理第7天和第14天时对盲肠内容物菌群结构和多样性的影响。结果表明：1）试验第7天，30%RH组肉鸡盲肠DGGE条带数（菌群丰富程度）高于60%RH组，而85%RH组低于60%RH组；试验第14天，30%、85%RH组肉鸡盲肠DGGE条带数均高于60%RH组。2）聚类分析显示，试验第7天，85%RH对盲肠菌群影响明显；试验第14天，30%RH对盲肠菌群影响明显。但随着处理时间推移，30%RH对肉鸡盲肠菌群影响越大。3）间歇性26℃环境下不同RH组肉鸡盲肠内共性菌群是Faecalibacterium prausnitzii；在试验第7天，30%、85%RH组肉鸡盲肠中特异性菌群是Stomatobaculum longum。结果提示：间歇性26℃环境下，低湿（30%RH）和高湿（85%RH）影响肉鸡盲肠菌群的结构和多样性，且不同处理时间RH的影响不同。

关键词： 相对湿度; 间歇热; 肉鸡; 盲肠菌群; 变形梯度凝胶电泳

本文引用格式

彭骞骞, 周莹, 王雪敏, 冯京海, 甄龙, 张少帅, 常玉, 石玉祥, 张敏红 . 不同相对湿度对间歇性26℃环境下肉鸡盲肠菌群多样性的影响[J]. 动物营养学报, 2017 , 29(5) : 1527 -1534 . DOI: 10.3969/j.issn.1006-267x.2017.05.010

Abstract

This study was carried out to investigate the effects of different relative humidity (RH) on cecal microflora diversity of broilers under intermittent 26℃ environment. One hundred and eighty 29-day-old Arbor Acres (AA) broilers were assigned to three environment chambers (RH were 30%, 60% and 85%, respectively), each chamber contained six cages with ten birds per cage (five males and five females), and each cage as a replicate. When broilers were 29 days of age, the temperature of groups was at 26℃, regulating the RH to 30%, 60% and 85%, and the temperature and RH of groups were kept six hours each day at 10:00 to 16:00, and broilers were kept at 21℃ and 60% RH in the other time. The trial period lasted for 14 days. The effects of RH on bacterial community and diversity in the cecal digesta of broilers at the 7th and 14th day under intermittent 26℃ environment were analyzed by using 16S rDNA-based denaturing gradient gel electrophoresis (DGGE), combined with the cloning and sequencing of DNA recycled by specificity and generality stripe tapping. The results showed as follows: 1) on the 7th day, cecum DGGE bands number (flora abundance) of broilers in 30% RH group was higher than that in 60% RH group, while in 85% RH group was lower than that in 60% RH group; on the 14th day, cecum DGGE bands number of broilers in 30% and 85% RH groups was higher than that in 60% RH group. 2) Cluster analysis showed that on the 7th day, 85% RH affected the cecal microflora obviously, but on the 14th day, 30% RH did. And as treatment time processing, 30% RH made a greater effect on cecal microflora. 3) The common cecal microbiota of different RH groups under intermittent 26℃ environment was Faecalibacterium prausnitzii, and on the 7th day, the specific cecal microbiota in 30% and 85% RH groups was Stomatobaculum longum. In conclusion, at the intermittent 26℃ environment, 30% and 85% RH can change the structure and diversity of cecal microflora of broilers, and the effects of RH at different processing time are different.

Key words： relative humidity; intermittent partial heat environment; broilers; cecal microbiota; denaturing gradient gel electrophoresis

参考文献

[1] MACKIE R,WHITE B,ISAACSON R E.Gastrointestinal microbiology:gastrointestinal microbes and host interactions[M].New York:Springer,1997.

[2] LUMPKINS B S,BATAL A B,LEE M D.Evaluation of the bacterial community and intestinal development of different genetic lines of chickens[J].Poultry Science,2010,89(8):1614-1621.  

[3] NICHOLSON J K,HOLMES E,KINROSS J,et al.Host-gut microbiota metabolic interactions[J].Science,2012,336(6086):1262-1267.  

[4] SJÖGREN Y M,TOMICIC S,LUNDBERG A,et al.Influence of early gut microbiota on the maturation of childhood mucosal and systemic immune responses[J].Clinical & Experimental Allergy,2009,39(12):1842-1851.  

[5] SAMUEL B S,SHAITO A,MOTOIKE T,et al.Effects of the gut microbiota on host adiposity are modulated by the short-chain fatty-acid binding G protein-coupled receptor,Gpr41[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(43):16767-16772.  

[6] 贺绍君,赵书景,李静,等.甜菜碱对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响[J].动物营养学报,2014,26(12):3731-3739.

[7] 王丽凤,张家超,马晨,等.鸡肠道微生物研究进展[J].动物营养学报,2013,25(3):494-502.

[8] BARROW P A.Probiotics for chickens[M]//FULLER R.Probiotics:the scientific basis.Netherlands:Springer,1992:225-257.

[9] BARNES E M,MEAD G C,BARNUML D A,et al.The intestinal flora of the chicken in the period 2 to 6 weeks of age,with particular reference to the anaerobic bacteria[J].British Poultry Science,1972,13(3):311-326.  

[10] HOOPER L V,WONG M H,THELIN A,et al.Molecular analysis of commensal host-microbial relationships in the intestine[J].Science,2001,291(5505):881-884.  

[11] 彭骞骞,王雪敏,张敏红,等.持续偏热环境对肉鸡盲肠菌群多样性的影响[J].中国农业科学,2016,49(1):186-194.

[12] 周莹,彭骞骞,张敏红,等.相对湿度对间歇性偏热环境下肉鸡体温、酸碱平衡及生产性能的影响[J].动物营养学报,2015,27(12):3726-3735.

[13] TOROK V A,OPHEL-KELLER K,LOO M,et al.Application of methods for identifying broiler chicken gut bacterial species linked with increased energy metabolism[J].Applied and Environmental Microbiology,2008,74(3):783-791.  

[14] GONG J,YU H,LIU T,et al.Effects of zinc bacitracin,bird age and access to range on bacterial microbiota in the ileum and caeca of broiler chickens[J].Journal of Applied Microbiology,2008,104(5):1372-1382.  

[15] FONSECA B B,BELETTI M E,SILVA M S D,et al.Microbiota of the cecum,ileum morphometry,pH of the crop and performance of broiler chickens supplemented with probiotics[J].Revista Brasileira De Zootecnia,2010,39(8):1756-1760.  

[16] LU J,IDRIS U,HARMON B,et al.Diversity and succession of the intestinal bacterial community of the maturing broiler chicken[J].Applied and Environmental Microbiology,2003,69(11):6816-6824.  

[17] AMIT-ROMACH E,SKLAN D,UNI Z.Microflora ecology of the chicken intestine using 16S ribosomal DNA primers[J].Poultry Science,2004,83(7):1093-1098.  

[18] 李永洙,李进,张宁波,等.热应激环境下蛋鸡肠道微生物菌群多样性[J].生态学报,2015,35(5):1601-1609.

[19] 李永洙.利用PCR-DGGE方法分析不同鸡群的盲肠微生物菌群结构变化[J].生态学报,2011,31(21):6513-6521.

[20] 张敏红,苏红光,冯京海,等.采集用于建立肉鸡生活环境舒适性评价模型数据的方法和专用装置:中国,CN103404447A[P].2013-11-27.

[21] 胡春红,张敏红,冯京海,等.偏热刺激对肉鸡休息行为、生理及生产性能的影响[J].动物营养学报,2015,27(7):2070-2076.

[22] 甄龙,石玉祥,张敏红,等.持续偏热环境对肉鸡生长性能、糖脂代谢及解偶联蛋白mRNA表达的影响[J].动物营养学报,2015,27(7):2060-2069.

[23] SHEN W H.Plant molecular biology,a laboratory manual:edited by M.S. Clark,Springer Verlag,Berlin,1997,DM 120.00[J].Plant Science,1997,124(2):223.

[24] MUYZER G,DE WAAL E C,UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.

[25] VAN ORSOUW N J,LI D,VIJG J.Denaturing gradient gel electrophoresis (DGGE) increases resolution and informativity of Alu-directed inter-repeat PCR[J].Molecular and Cellular Probes,1997,11(2):95-101.  

[26] ZHOU H,GONG J,BRISBIN J T,et al.Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics,using the polymerase chain reaction-denaturing gradient gel electrophoresis technique[J].Poultry Science,2007,86(12):2541-2549.  

[27] GONG J H,SI W D,FORSTER R J,et al.16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts:from crops to ceca[J].FEMS Microbiology Ecology,2007,59(1):147-157.  

[28] 倪学勤,GONG J,YU H,等.采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性[J].畜牧兽医学报,2008,39(7):955-961.

[29] 姚琨,张日俊.肉仔鸡后肠道菌群多样性及其演替规律的研究[C]//中国畜牧兽医学会动物营养学分会第十次学术研讨会论文集.杭州:中国畜牧兽医学会,2008:1.

[30] ADAMS R L,ROGLER J C.The effects of dietary aspirin and humidity on the performance of light and heavy breed chicks[J].Poultry Science,1968,47(4):1344-1348.  

[31] SIZOVA M V,MULLER P,PANIKOV N,et al.Stomatobaculum longum gen. nov.,sp. nov.,an obligately anaerobic bacterium from the human oral cavity[J].International Journal of Systematic and Evolutionary Microbiology,2013,63(4):1450-1456.

[32] SOKOL H,PIGNEUR B,WATTERLOT L,et al.Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(43):16731-16736.  

[33] LOUIS P,FLINT H J.Diversity,metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine[J].FEMS Microbiology Letters,2009,294(1):1-8.  

[34] SRIDHAR J,EITEMAN M A.Metabolic flux analysis of Clostridium thermosuccinogenes[J].Applied Biochemistry and Biotechnology,2001,94(1):51-69.  

[35] DUNCAN S H,HOLD G L,HARMSEN H J M,et al.Growth requirements and fermentation products of Fusobacterium prausnitzii,and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov.,comb. nov[J].International Journal of Systematic and Evolutionary Microbiology,2002,52(6):2141-2146.

[36] BEN-AMOR K,HEILIG H,SMIDT H,et al.Genetic diversity of viable,injured,and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis[J].Applied and Environmental Microbiology,2005,71(8):4679-4689.  

[37] PRYDE S E,DUNCAN S H,HOLD G L,et al.The microbiology of butyrate formation in the human colon[J].FEMS Microbiology Letters,2002,217(2):133-139.  
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