动物营养学报 >

2017 , Vol. 29 >Issue 5: 1600 - 1609

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.019

饲料营养 Feed science and technology

胱氨酸类硒源对产蛋鸡蛋品质、抗氧化能力和蛋中硒含量的影响

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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 生物饲料开发国家工程研究中心, 北京 100081
龙烁(1993-),女,山东临沂人,硕士研究生,从事家禽营养与饲料科学研究。E-mail:maplega@163.com

收稿日期: 2016-11-18

  网络出版日期: 2017-05-13

基金资助

现代农业产业技术体系（CARS-41-K13）；家禽产业技术体系北京市创新团队（CARS-PSTP）

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Effects of Cystine Selenium Sources on Egg Quality, Antioxidant Capacity and Egg Selenium Content of Laying Hens

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  • Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China

Received date: 2016-11-18

  Online published: 2017-05-13

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摘要

本试验旨在研究饲粮中添加硒代胱氨酸（SeC）、L-硒甲基硒代半胱氨酸（L-MSC）、L-硒代半胱氨酸（L-SeCys）和亚硒酸钠（SS）对产蛋鸡生产性能、蛋品质、抗氧化能力和蛋中硒含量的影响，以期为富硒鸡蛋的生产提供依据。选用26周龄体况良好、产蛋率接近的海兰灰蛋鸡450只，随机分为5个组，每组6个重复，每个重复15只鸡。对照组饲喂基础饲粮（不额外补充硒），其他4组饲粮在基础饲粮中添加0.30 mg/kg硒，分别来自SeC、L-MSC、L-SeCys和SS，各组饲粮中硒含量实测值分别为0.08（对照组）、0.36（SeC组）、0.35（L-MSC组）、0.31（L-SeCys组）和0.37（SS组）mg/kg。预试期1周，正试期4周。结果表明：1）与SS组和对照组相比，胱氨酸类硒源各组产蛋鸡的平均蛋重、产蛋率和料蛋比均无显著差异（P>0.05）。L-SeCys组平均日采食量在试验1~2周时显著低于对照组（P<0.05）。2）与SS组和对照组相比，胱氨酸类硒源各组产蛋鸡的蛋白高度、哈氏单位、蛋壳强度、蛋形指数、蛋黄和蛋白比例均无显著差异（P>0.05）。试验第4周末，SS组蛋黄颜色显著高于其他各组（P<0.05）；SeC和SS组蛋壳厚度显著高于其他各组（P<0.05），L-MSC和L-SeCys组蛋壳厚度显著高于对照组（P<0.05）；对照组蛋壳比例显著低于各试验组（P<0.05）。3）与SS组和对照组相比，胱氨酸类硒源各组产蛋鸡的血浆谷胱甘肽过氧化物酶（GSH-Px）活性显著升高（P<0.05）；SeC和SS组血浆总超氧化物歧化酶（T-SOD）活性显著高于对照组（P<0.05）；胱氨酸类硒源各组血浆丙二醛（MDA）含量与SS组相比无显著差异（P>0.05）；对照组血浆总抗氧化能力（T-AOC）显著低于其他各组（P<0.05），L-MSC组的血浆T-AOC最高，显著高于SS组（P<0.05）。4）与对照组相比，饲粮中添加胱氨酸类硒源显著提高了蛋中硒含量（P<0.05），其中L-MSC组最高；胱氨酸类硒源各组蛋中硒含量和硒转化率均高于SS组（P>0.05）。由此可见，饲粮中添加3种胱氨酸类硒源可增强机体抗氧化水平，提高蛋中硒含量，其中SeC和L-MSC效果更好。

关键词： 蛋鸡; 硒源; 抗氧化能力; 蛋中硒含量

本文引用格式

龙烁, 张海军, 武书庚, 王晓翠, 王晶, 齐广海 . 胱氨酸类硒源对产蛋鸡蛋品质、抗氧化能力和蛋中硒含量的影响[J]. 动物营养学报, 2017 , 29(5) : 1600 -1609 . DOI: 10.3969/j.issn.1006-267x.2017.05.019

Abstract

This study was conducted to investigate the effects of dietary selenocystine (SeC), L-Se-methyselenocysteine (L-MSC), L-selencysteine (L-SeCys) and sodium selenite (SS) on performance, egg quality, antioxidant capacity and egg selenium content of laying hens, with the aim to provide basis for the production of selenium enriched eggs. Four hundred and fifty healthy 26-week-old Hy-Line Grey laying hens with similar laying rate were randomly allotted into 5 groups with 6 replicates per group and 15 hens per replicate. The laying hens in control group were fed a basal diet without adding exogenous selenium source, and the other four groups were fed the basal diet supplemented with SeC, L-MSC, L-SeCys or SS containing 0.30 mg/kg selenium. The measured values of selenium content in diets were 0.08 (control group), 0.36 (SeC group), 0.35 (L-MSC group), 0.31 (L-SeCys group) and 0.37 (SS group) mg/kg, respectively. The pre-test period lasted for one week and the test period lasted for four weeks. The results showed as follows: 1)compared with SS and control groups, the average egg weight, laying rate and the ratio of feed to egg of laying hens in all cystine selenium source groups had no significant differences (P>0.05). The average daily feed intake (ADFI) in L-SeCys group was significantly lower than that in control group during 1 to 2 weeks (P<0.05). 2) Compared with SS and control groups, the albumen height, Haugh unit, eggshell strength, egg shape index and the percentages of yolk and albumen of laying hens in all cystine selenium source groups had no significant differences (P>0.05). At the end of 4th week, the yolk color in SS group was significantly higher than that in the other groups (P<0.05); the eggshell thickness in SeC and SS groups was significantly higher than that in the other groups (P<0.05), and the eggshell thickness in L-MSC and L-SeCys groups was significantly higher than that in control group (P<0.05); the egg shell percentage in control group was significantly lower than that in the other groups (P<0.05). 3) Compared with SS and control groups, the activity of glutathione peroxidase (GSH-Px) in plasma of laying hens in all cystine selenium source groups was significantly increased (P<0.05). The activity of total superoxide dismutase activity (T-SOD) in plasma in SeC and SS groups was significantly higher than that in control group (P<0.05). There was no significant difference in the content of malondialdebyde (MDA) in plasma between all cystine selenium source groups and SS group (P>0.05). The total antioxidant capacity (T-AOC) in plasma in control group was significantly lower than that in the other groups (P<0.05), and the T-AOC in L-MSC group was highest and was significantly higher than that in SS group (P<0.05). 4)Compared with control group, dietary cystine selenium sources significantly increased egg selenium content (P<0.05), and egg selenium content in L-MSC group was the highest. The egg selenium content and selenium conversion rate in all cystine selenium source groups were higher than that in SS group (P>0.05). In conclusion, dietary three cystine selenium sources can improve the antioxidant capacity and egg selenium content, and SeC and L-MSC have the better effects.

Key words： laying hens; selenium sources; antioxidant capacity; egg selenium content

参考文献

[1] LI F,LUTZ P B,PEPELYAYEVA Y,et al.Redox active motifs in selenoproteins[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(19):6976-6981.  

[2] SURAI P F,FISININ V I.Selenium in poultry breeder nutrition:an update[J].Animal Feed Science and Technology,2014,191:1-15.

[3] BROZMANOVÁ J,MÁNIKOVÁ D,VL?KOVÁ V,et al.Selenium:a double-edged sword for defense and offence in cancer[J].Archives of Toxicology,2010,84(12):919-938.  

[4] CHEN T F,WONG Y S.Selenocystine induces caspase-independent apoptosis in MCF-7 human breast carcinoma cells with involvement of p53 phosphorylation and reactive oxygen species generation[J].The International Journal of Biochemistry & Cell Biology,2009,41(3):666-676.  

[5] SONG Y X,HOU J X,ZHANG L,et al.Effect of dietary selenomethionine supplementation on growth performance,tissue se concentration,and blood glutathione peroxidase activity in kid boer goats[J].Biological Trace Element Research,2015,167(2):242-250.  

[6] 刘建群,赵元,张锐,等.新型营养强化剂L-硒甲基硒代半胱氨酸的研究进展[J].中国食品添加剂,2011(2):152-156.

[7] DELEZIE E,ROVERS M,VAN DER AA A,et al.Comparing responses to different selenium sources and dosages in laying hens[J].Poultry Science,2014,93(12):3083-3090.  

[8] JING C L,DONG X F,WANG Z M,et al.Comparative study of DL-selenomethionine vs. sodium selenite and seleno-yeast on antioxidant activity and selenium status in laying hens[J].Poultry Science,2015,94(5):965-975.  

[9] SK?IVAN M, ŠIMÁN ě J, DLOUHÁ G,et al.Effect of dietary sodium selenite,Se-enriched yeast and Se-enriched Chlorella on egg Se concentration,physical parameters of eggs and laying hen production[J].Czech Journal of Animal Science,2006,51(4):163-167.

[10] LEESON S,NAMKUNG H,CASTON L,et al.Comparison of selenium levels and sources and dietary fat quality in diets for broiler breeders and layer hens[J].Poultry Science,2008,87(12):2605-2612.  

[11] PAYNE R L,LAVERGNE T K,SOUTHERN L.Effect of inorganic versus organic selenium on hen production and egg selenium concentration[J].Poultry Science,2005,84(2):232-237.  

[12] RICHIE J P JR,DAS A,CALCAGNOTTO A M,et al.Comparative effects of two different forms of selenium on oxidative stress biomarkers in healthy men:a randomized clinical trial[J].Cancer Prevention Research,2014,7(8):796-804.  

[13] UTTERBACK P L,PARSONS C M,YOON I,et al.Effect of supplementing selenium yeast in diets of laying hens on egg selenium content[J].Poultry Science,2005,84(12):1900-1901.  

[14] PAN C L,ZHAO Y X,LIAO S F,et al.Effect of selenium-enriched probiotics on laying performance,egg quality,egg selenium content,and egg glutathione peroxidase activity[J].Journal of Agricultural and Food Chemistry,2011,59(21):11424-11431.  

[15] 孙庆艳,武书庚,张海军,等.饲粮中添加不同硒源对产蛋鸡生产性能和抗氧化能力的影响[J].动物营养学报,2016,28(4):1177-1185.

[16] URSINI F,HEIM S,KIESS M,et al.Dual function of the selenoprotein PHGPx during sperm maturation[J].Science,1999,285(5432):1393-1396.  

[17] 瞿祥虎,黄开勋,高中洪,等.谷胱甘肽过氧化物酶的硒代半胱氨酸插入元件[J].中国生物化学与分子生物学学报,1999,15(2):274-278.

[18] 邱榕生,呙于明.有机镁与有机硒对肉鸡机体组织抗氧化机能的影响[J].畜牧兽医学报,2003,34(6):542-547.

[19] MOORE M A,WANDERA R C,XIA Y M,et al.Selenium supplementation of Chinese women with habitually low selenium intake increases plasma selenium,plasma glutathione peroxidase activity,and milk selenium,but not milk glutathione peroxidase activity[J].The Journal of Nutritional Biochemistry,2000,11(6):341-347.  

[20] BENNETT D C,CHENG K M.Selenium enrichment of table eggs[J].Poultry Science,2010,89(10):2166-2172.  

[21] HANTIRATIKUL A,CHINRASRI O, CHANTIRATIKUL P.Effect of sodium selenite and zinc-L-selenomethionine on performance and selenium concentrations in eggs of laying hens[J].Asian-Australasian Journal of Animal Sciences,2008,21(7):1048-1052.  

[22] ORT J F,LATSHAW J D.The toxic level of sodium selenite in the diet of laying chickens[J].Journal of Nutrition,1978,108(7):1114-1120.

[23] LEVANDER O A.Selenium requirements as discussed in the 1996 joint FAO/IAEA/WHO expert consultation on trace elements in human nutrition[J].Biomedical and Environmental Sciences,1997,10(2/3):214-219.

[24] NRC.Recommended dietary allowances[S].10th ed.Washington,D. C.:National Academy Press, 1989.

[25] 胡华锋,黄炎坤,介晓磊,等.3种硒源对蛋鸡生产性能、蛋硒含量及转化率的影响[J].动物营养学报,2013,25(7):1603-1609.

[26] OLIVEIRA T F B,RIVERA D F R,MESQUITA F R,et al.Effect of different sources and levels of selenium on performance,meat quality,and tissue characteristics of broilers[J].Journal of Applied Poultry Research,2014,23(1):15-22.  

[27] UNNI E,KITTRELL F S,SINGH U,et al.Osteopontin is a potential target gene in mouse mammary cancer chemoprevention by Se-methylselenocysteine[J].Breast Cancer Research,2004,6(5):586-592.  
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