动物营养学报 >

2017 , Vol. 29 >Issue 5: 1750 - 1758

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.035

研究简报 Short communications

油脂类型对肉鸡不同组织甘油醛-3-磷酸脱氢酶基因相对表达量的影响

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  • 河南农业大学饲料营养河南省工程实验室, 河南农业大学国家家养动物种质资源平台, 郑州 450002
陶亚飞(1990-),男,河南商丘人,硕士研究生,动物遗传育种与繁殖专业。E-mail:15515517571@163.com

收稿日期: 2016-11-17

  网络出版日期: 2017-05-13

基金资助

国家自然科学基金（31272434）

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Effects of Oil Types on Glyceraldehyde-3-Phosphate Dehydrogenase Gene Relative Expression Level in Different Tissues of Broilers

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  • Henan Agricultural University National Germplasm Resoueces Platform for Animals, Feed Nutrition Engineering Laboratory of Henan Province, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2016-11-17

  Online published: 2017-05-13

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摘要

本试验旨在探究油脂类型对肉鸡不同组织甘油醛-3-磷酸脱氢酶（GAPDH）基因相对表达量的影响。试验选用240只1日龄的科宝肉鸡母雏，随机分为8个组（4个单一油脂组，分别在饲粮中添加5.00%亚麻油、玉米油、芝麻油和猪油；4个混合油脂组，分别在饲粮中添加2.50%猪油+2.50%玉米油、2.50%猪油+2.50%芝麻油、2.50%亚麻油+2.50%玉米油和2.50%亚麻油+2.50%芝麻油），每组6个重复，每个重复5只鸡。试验期42 d。结果表明：1）组织及油脂类型与组织的交互作用对42日龄肉鸡组织GAPDH基因的相对表达量影响显著（P<0.05），油脂类型对42日龄肉鸡组织GAPDH基因的相对表达量的影响不显著（P>0.05）。42日龄肉鸡胸肌GAPDH基因的相对表达量显著高于肝脏和腹脂（P<0.05），是肝脏的37.50~89.50倍，是腹脂的129.54~190.64倍，而42日龄肉鸡的GAPDH基因的相对表达量在肝脏与腹脂之间差异不显著（P>0.05）；玉米油组胸肌中GAPDH基因的相对表达量显著高于猪油组（P<0.05）。2）21日龄肉鸡肝脏GAPDH基因的相对表达量显著或极显著高于42日龄（P<0.05或P<0.01）。3）油脂组合及油脂组合与日龄的交互作用对肉鸡肝脏GAPDH基因相对表达量的影响均不显著（P>0.05），但日龄对肉鸡肝脏GAPDH基因相对表达量的影响显著（P<0.05）。由此可见，油脂类型对肉鸡GAPDH基因的相对表达量的影响呈现组织间的差异，玉米油可提高胸肌GAPDH基因的相对表达量。42日龄肉鸡胸肌GAPDH基因的相对表达量显著高于肝脏和腹脂，21日龄肉鸡肝脏GAPDH基因的相对表达量显著高于42日龄。

关键词： 肉鸡; 油脂; GAPDH; 基因表达

本文引用格式

陶亚飞, 陈青, 徐媛媛, 石秀文, 陈文, 黄艳群 . 油脂类型对肉鸡不同组织甘油醛-3-磷酸脱氢酶基因相对表达量的影响[J]. 动物营养学报, 2017 , 29(5) : 1750 -1758 . DOI: 10.3969/j.issn.1006-267x.2017.05.035

Abstract

This experiment was conducted to study the effects of oil types on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene relative expression level in different tissues of broilers. A total of 240 one-day-old female Cobb broilers were selected and randomly assigned to 8 groups (four single oil groups were fed diets added with 5.00% linseed oil, corn oil, sesame oil and lard oil, respectively; four oil combination groups were fed diets added with 2.50% lard oil+2.50% corn oil, 2.50% lard oil+2.50% sesame oil, 2.50% linseed oil+2.50% corn oil and 2.50% linseed oil+2.50% sesame oil, respectively) with 6 replicates per group and 5 broilers per replicate. The experiment lasted for 42 days. The results showed as follows: 1) the relative expression level of GAPDH gene in broiler tissues was significantly affected by tissues and the interaction between oil types and tissues (P<0.05), however, the effects of oil types were not significant (P>0.05). The relative expression level in pectorals of GAPDH gene of 42-day-old broilers was significantly higher than that in liver and abdominal fat (P<0.05), the relative expression level of GAPDH gene in liver was 37.50 to 89.50 and 129.54 to 190.64 times than that in liver and abdominal fat, respectively, however, the relative expression level of GAPDH gene between liver and abdominal fat was not significant (P>0.05). The relative expression level of GAPDH gene in corn oil group was significantly higher than that in sesame oil group in pectorals (P<0.05). 2) The relative expression level of GAPDH gene in liver of 21-day-old broilers was significantly higher than that of 42-day-old broilers (P<0.05 or P<0.01). 3) The relative expression level of GAPDH gene in liver of broilers was significantly affected by days of age (P<0.05), however, the effects of oil combination and the interaction between oil combination and days of age were not significant (P>0.05). In conclusion, the effect of oil types on the relative expression level of GAPDH gene in broilers shows difference among different tissues, corn oil can improve the relative expression level of GAPDH gene in pectorals. The relative expression level in pectorals of GAPDH gene of 42-day-old broilers is significantly higher than that in liver and abdominal fat, and the relative expression level of GAPDH gene in liver of 21-day-old broilers is significantly higher than that of 42-day-old broilers.

Key words： broilers; oil; GAPDH; gene expression

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