研究简报 Short communications

酵母培养物对生长期锦江黄牛生产性能、抗氧化能力及免疫性能的影响

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  • 1. 江西农业大学江西省动物营养重点实验室/营养饲料开发研究中心, 南昌 330045;
    2. 高安裕丰农牧有限公司, 高安 330800
陈作栋(1991-),男,江苏镇江人,硕士研究生,从事反刍动物营养研究。E-mail:289411078@qq.com

收稿日期: 2016-11-17

  网络出版日期: 2017-05-13

基金资助

国家公益性行业(农业)科研专项(201303143);广昌县肉牛饲料研制与营养(09005392);国家现代肉牛牦牛产业技术体系项目(CAR-38);江西省研究生创新专项(YC2015-S178)

Effects of Yeast Culture on Performance, Antioxidant Capacity and Immune Function of Jinjiang Yellow Cattle during Growing Period

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  • 1. Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Gao'an Yufeng Agricultural and Livestock Co., Ltd., Gao'an 330800, China

Received date: 2016-11-17

  Online published: 2017-05-13

摘要

本研究旨在探讨饲粮中添加酵母培养物对生长期锦江黄牛生产性能、抗氧化能力及免疫性能的影响,为生长期肉牛培育及锦江黄牛饲粮配制提供依据。试验选择16头6月龄、体重140 kg左右的锦江黄牛公犊,随机分成对照组和试验组,每组8头。对照组饲喂基础饲粮,试验组在对照组饲粮基础上添加30 g/(d·头)酵母培养物,试验期为60 d。结果表明:1)试验组平均日增重显著高于对照组(P<0.05);2)试验组饲粮干物质和粗蛋白质的表观消化率显著高于对照组(P<0.05),但饲粮有机物、中性洗涤纤维和酸性洗涤纤维的表观消化率相比于对照组则差异不显著(P>0.05);3)试验第30天和第60天,试验组血清总超氧化物歧化酶(T-SOD)活性显著高于对照组(P<0.05),而血清丙二醛(MDA)含量较对照组有所降低,但差异不显著(P>0.05),同时血清谷胱甘肽过氧化物酶(GSH-Px)活性也与对照组无显著差异(P>0.05);4)试验组血清免疫球蛋白A(IgA)含量在试验第30天显著高于对照组(P<0.05),血清免疫球蛋白G(IgG)含量在试验第30天和第60天相比对照组均有一定程度的提高,但差异不显著(P>0.05),血清免疫球蛋白M(IgM)含量在试验第30天和第60天与对照组相比无显著差异(P>0.05)。由上述结果可知,饲粮中添加30 g/(d·头)酵母培养物可促进饲粮中养分的消化,增加生长期锦江黄牛的抗氧化能力和免疫性能,进而改善其生产性能。

本文引用格式

陈作栋, 周珊, 赵向辉, 杨食堂, 瞿明仁, 文露华, 许兰娇 . 酵母培养物对生长期锦江黄牛生产性能、抗氧化能力及免疫性能的影响[J]. 动物营养学报, 2017 , 29(5) : 1767 -1773 . DOI: 10.3969/j.issn.1006-267x.2017.05.037

Abstract

This study was conducted to investigate the effects of yeast culture on performance, antioxidant capacity and immune function of Jinjiang cattle during growing period, which would provide technical support for the diet formulation of Jinjiang cattle and the breeding of growing cattle. Sixteen 6-month-old Jinjiang cattle with about 140 kg body weight were randomly divided into 2 groups including control group and experimental group, and each group had 10 cattle. The cattle in the control group were fed a basal diet. For experimental group, yeast culture (30 g per cattle per day) was added into the basal diet. The experiment lasted for 60 days. The results showed as follows: 1) the average daily gain in the experimental group was significantly higher than that in the control group (P<0.05). 2) Compared with the control group, the apparent digestibility of dry matter (DM) and crude protein (CP) in the experimental group was significantly increased (P<0.05), but there was no significant difference in the apparent digestibility of organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) between control group and experimental group (P>0.05). 3)On day 30 and 60 of experiment, compared with the control group, the activity of serum total superoxide dismutase (T-SOD) in the experimental group was significantly increased (P<0.05), the serum malondialdehyde (MDA) content in the experimental group was decreased, but the difference was not significant (P>0.05), and the activity of serum glutathione peroxidase (GSH-Px) in the experimental group had no significant difference (P>0.05). 4) Serum immunoglobulin (Ig) A content on day 30 of experiment in experimental group was significantly higher than that in control group (P<0.05), and serum IgG content on day 30 and 60 of experiment in experimental group had the trend of increase compared with control group, but the difference was not significant (P>0.05). In addition, there was no significant difference in serum IgM content on day 30 and 60 of experiment between control group and experimental group (P>0.05). In conclusion, diet supplemented with 30 g yeast culture per day per cattle can promote the digestion of nutrients in the diet, enhance the antioxidant capacity and immune function, and then improve the performance of Jinjiang cattle.

参考文献

[1] 郭勇庆,张英杰.酵母培养物在反刍动物上的应用[J].北方牧业,2008(16):9.

[2] 张鹏飞,于安乐,高巍.酵母培养物对反刍动物作用的研究进展[J].吉林畜牧兽医,2007,28(6):17-20.

[3] 易力,汪洋.饲用微生态制剂——酵母培养物及其在水产养殖中的应用[J].饲料博览,2009(9):25-27.

[4] 周淑芹,孙文志.酵母培养物[J].中国饲料,2003(5):31-32.

[5] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993.

[6] TAKEMURA G,ONODERA T,ASHRAF M.Characterization of exogenous hydroxyl radical effects on myocardial function,metabolism and ultrastructure[J].Journal of Molecular and Cellular Cardiology,1994,26(4):441-454.  

[7] SEDLAK J,LINDSAY R H.Estimation of total,protein-bound,and nonprotein sulfhydryl groups in tissue with Ellman's reagent[J].Analytical Biochemistry,1968,25:192-205.

[8] KOO D D H,WELSH K I,WEST N E J,et al.Endothelial cell protection against ischemia/reperfusion injury by lecithinized superoxide dismutase[J].Kidney International,2001,60(2):786-796.  

[9] 王东明.酵母培养物对肉鸡消化与免疫调节影响的研究[D].硕士学位论文.长春:吉林农业大学,2007.

[10] OLSON K C,CATON J S,KIRBY D R,et al.Influence of yeast culture supplementation and advancing season on steers grazing mixed-grass prairie in the northern Great Plains:Ⅱ.Ruminal fermentation,site of digestion,and microbial efficiency[J].Journal of animal science,1994,72(8):2158-2170.

[11] ERASMUS L J,BOTHA P M,KISTNER A.Effect of yeast culture supplement on production,rumen fermentation,and duodenal nitrogen flow in dairy cows[J].Journal of Dairy Science,1992,75(11):3056-3065.  

[12] EL HASSAN S M,NEWBOLD C J,EDWARDS I E,et al.Effect of yeast culture on rumen fermentation,microbial protein flow from the rumen and live-weight gain in bulls given high cereal diets[J].Animal Science,1996,62(1):43-48.  

[13] 汤飞飞.母猪妊娠后期和仔猪日粮添加酵母培养物对母猪生产性能、仔猪肠道健康及免疫机能的影响[D].硕士学位论文.南京:南京农业大学,2012.

[14] 张连忠.酵母培养物对雏鸡生长性能、免疫器官发育和血清相关激素的影响[J].中国畜牧兽医,2011,38(4):33-37.

[15] ROBINSON P H.Effect of yeast culture (Saccharomyces cerevisiae) on adaptation of cows to diets postpartum[J].Journal of Dairy Science,1997,80(6):1119-1125.  

[16] DENEV S A,PEEVA T,RADULOVA P,et al.Yeast cultures in ruminant nutrition[J].Bulgarian Journal of Agricultural Science,2007,13:357-374.

[17] 寇慧娟,陈玉林,刘敬敏,等.酵母培养物对羔羊生产性能、营养物质表现消化率及瘤胃发育的影响[J].西北农林科技大学学报:自然科学版,2011,39(8):45-50.

[18] MCGILLIARD M L,STALLINGS C C.Increase in milk yield of commercial dairy herds fed a microbial and enzyme supplement[J].Journal of Dairy Science,1998,81(5):1353-1357.  

[19] 张昌吉.饲粮中添加酵母培养物对绵羊瘤胃发酵及养分消化代谢的影响[D].硕士学位论文.兰州:甘肃农业大学,2005.

[20] CHAUCHEYRAS-DURAND F,MASSÉGLIA S,FONTY G.Effect of the microbial feed additive Saccharomyces cerevisiae CNCM I-1077 on protein and peptide degrading activities of rumen bacteria grown in vitro[J].Current Microbiology,2005,50(2):96-101.  

[21] 肖曼,高振华,张少成,等.酵母培养物对肉仔鸡免疫功能、抗氧化及血清生化指标的影响[J].广东农业科学,2013,40(5):103-106.

[22] 张培松,周玉香,王洁.三种日粮添加物对滩羊血液抗氧化性能的影响[J].家畜生态学报,2013,34(6):44-47.

[23] FLOHÉ L,GÜNZLER W A.Assays of glutathione peroxidase[J].Methods in Enzymology,1984,105:114-120.

[24] 王兰惠.灌注酵母培养物对绵羊血液生化指标、免疫及抗氧化功能的影响[D].硕士学位论文.长春:吉林农业大学,2015.

[25] 张学峰,王兰惠,周雪飞,等.瘤胃灌注酵母培养物对绵羊血液生化指标和免疫及抗氧化功能的影响[J].中国畜牧杂志,2016,52(9):62-65.

[26] 熊皓平,杨伟丽,张友胜,等.天然植物抗氧化剂的研究进展[J].天然产物研究与开发,2001,13(5):75-79.

[27] HOLDOM M D,LECHENNE B,HAY R J,et al.Production and characterization of recombinant Aspergillus fumigatus Cu,Zn superoxide dismutase and its recognition by immune human sera[J].Journal of Clinical Microbiology,2000,38(2):558-562.

[28] FAHEY R C.Novel thiols of prokaryotes[J].Annual Review of Microbiology,2001,55(1):333-356.  

[29] 刘丽,刘倩.酵母及其培养物在动物生产中的应用[J].上海畜牧兽医通讯,2012(4):57-58.

[30] 王学东,李彪,戴晋军.活性干酵母在养猪中的应用[J].养猪,2009(4):9-10.

[31] 范秀敏,陈翠玲,张慧,等.酵母培养物在仔猪生产中的应用[J].饲料博览,2012(10):33-35.

[32] ZHANG A Z,LU D X,DA C,et al.Effects of yeast culture on immune indices of cashmere goats[J].Chinese Journal of Animal Nutrition,2008,20(2):163-169.
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