动物营养学报 >

2017 , Vol. 29 >Issue 5: 1792 - 1802

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.040

研究简报 Short communications

蛋氨酸水平对羔羊体况发育、消化道组织形态及血清抗氧化指标的影响

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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081
王杰(1989-),男,山东临沂人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:nkywangjie@163.com

收稿日期: 2016-11-01

  网络出版日期: 2017-05-13

基金资助

国家公益性行业（农业）科研专项（201303143）；国家肉羊产业技术体系建设专项（CARS-39）

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Effects of Methionine Level on Development of Body Condition, Morphology of Digestive Tract and Serum Antioxidant Indexes in Lambs

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  • Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Beijing 100081, China

Received date: 2016-11-01

  Online published: 2017-05-13

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摘要

本试验旨在研究蛋氨酸水平对羔羊体况发育、消化道组织形态及血清抗氧化指标的影响。试验选取12对7日龄断母乳的湖羊双胞胎公羔，采用配对试验设计，分为对照（CON）组和低蛋氨酸（LM）组，1对双胞胎羔羊分到不同的组中。试验分2个阶段进行，第1阶段（8~56日龄），CON组羔羊饲喂基础代乳粉和基础开食料；LM组羔羊饲喂的代乳粉和开食料在CON组基础上分别全部扣除（0.70%和0.40%）额外添加的蛋氨酸，其余营养水平保持一致。第2阶段（57~84日龄），2组羔羊停止饲喂代乳粉且饲粮均为基础开食料。在56和84日龄，各选取6对双胞胎羔羊进行屠宰，分离消化道组织，采集血清样品。结果表明：1）在采食量方面，LM组羔羊对蛋氨酸采食量在8~56日龄、8~84日龄阶段均极显著低于CON组（P<0.01），而干物质采食量却均显著高于CON组（P<0.05）；在体尺指标方面，56日龄，LM组除体重、体斜长、胸围和体长指数显著低于CON组（P<0.05）外，其他体况发育指标2组间均无显著性差异（P>0.05）；84日龄，2组间体况发育指标均无显著性差异（P>0.05）。2）56日龄，LM组羔羊的瘤胃乳头宽度显著低于CON组（P<0.05），2组羔羊的其他消化道形态发育指标差异不显著（P>0.05）。3）56日龄，LM组羔羊血清中超氧化物歧化酶活性极显著低于CON组（P<0.01）；84日龄，LM组羔羊血清中除谷胱甘肽过氧化物酶活性显著低于CON组（P<0.05）外，2组羔羊的其他血清抗氧化指标差异均不显著（P>0.05）。结果提示，饲粮低蛋氨酸水平抑制了羔羊体况（体重、体斜长、胸围、体长指数）发育及瘤胃乳头宽度的增加，同时降低了机体血清中超氧化物歧化酶活性，然而却提高了干物质采食量；提高饲粮蛋氨酸水平后，羔羊体况发育及消化道组织形态发育也随之得到补偿，但机体抗氧化防御系统仍未得到完全改善。

关键词： 蛋氨酸; 羔羊; 体况发育; 消化道形态; 抗氧化指标

本文引用格式

王杰, 崔凯, 王世琴, 刁其玉, 张乃锋 . 蛋氨酸水平对羔羊体况发育、消化道组织形态及血清抗氧化指标的影响[J]. 动物营养学报, 2017 , 29(5) : 1792 -1802 . DOI: 10.3969/j.issn.1006-267x.2017.05.040

Abstract

The aim of this study was to assess the effects of methionine level on development of body condition, morphology of digestive tract and serum antioxidant indexes in lambs. Twelve pairs of male Hu twin lambs weaned at 7 days of age were selected and divided into two groups [control (CON) group and low methionine level (LM) group] with a matched-pairs design, and one pair of lambs were assigned to the different groups. The experiment consisted of two stages. Stage 1 (8 to 56 days of age), lambs in the CON group were fed basal milk replacer and basal starter, while those in the LM group were fed basal milk replacer and basal starter deducting 0.70% and 0.40% methionine on the basis of CON group, respectively. Stage 2 (57 to 84 days of age), all lambs were stop feeding milk replacer and fed basal starter. Six twins at 56 and 84 days of age were slaughtered to collect gastrointestinal tract tissues and serum sample. The results showed as follows: 1) for feed intake of methionine, LM group was significantly lower than CON group at 8 to 56 days of age and 8 to 84 days of age (P<0.01), but dry matter intake in LM group was significantly higher than that in CON group (P<0.05); at 56 days of age, no significant differences were observed in development of body condition indexes between groups (P>0.05) except that body weight, body length, heart girth, body length index in LM group were significantly lower than those in CON group (P<0.05); at 84 days of age, no significant differences were observed in development of body condition indexes between groups (P>0.05). 2) At 56 days of age, ruminal papillary width in LM group was significantly lower than that in CON group (P<0.05), but no significant differences were observed in indexes of morphology of the digestive tract between groups (P>0.05). 3) At 56 days of age, serum superoxide dismutase activity in LM group was significantly lower than that in CON group (P<0.01); at 84 days of age, no significant differences were observed in serum antioxidant indexes between groups (P>0.05) except that glutathione peroxidase activity in LM group was significantly lower than that in CON group (P<0.05). In conclusion, low methionine level of diet not only results in inhibiting the growth of body weight, body length, heart girth, body length index and ruminal papillary width, but also decreases serum superoxide dismutase activity, however, increases dry matter intake; after the improvement of dietary methionine level, a subsequent recovery appear in development of body condition and morphology development of digestive tract, but body's antioxidant system is not completely improved.

Key words： methionine; lamb; development of body condition; morphology of digestive tract; antioxidant indexes

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