动物营养学报 >

2017 , Vol. 29 >Issue 6: 1921 - 1929

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.06.013

反刍动物营养 Ruminant nutrition

稻草或玉米秸秆替代苜蓿对奶牛氮代谢、血清生理生化指标及肝脏和肾脏组织形态变化的影响

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  • 1. 奶牛营养学北京市重点实验室, 北京农学院动物科学技术学院, 北京 102206;
    2. 浙江大学动物科学学院奶业科学研究所, 杭州 310058
王炳(1989-),男,河南南阳人,博士,研究方向为奶牛营养与生理。E-mail:wbwz0810@126.com

收稿日期: 2016-12-07

  网络出版日期: 2017-06-07

基金资助

国家奶牛产业技术体系（CARS-37）

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Effects of Alfalfa Replacement by Rice Straw or Corn Stover on Nitrogen Metabolism, Serum Physiological-Biochemical Indices and Histomorphology of Liver and Kidney of Dairy Cows

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  • 1. Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Institute of Dairy Science, Zhejiang University, Hangzhou 310058, China

Received date: 2016-12-07

  Online published: 2017-06-07

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摘要

本试验旨在研究稻草或玉米秸秆替代苜蓿对奶牛氮代谢、血清生理生化指标及肝脏和肾脏组织形态变化的影响。选择45头泌乳中期的经产健康荷斯坦奶牛，随机分成3组，每组15头牛。各组饲喂含不同粗饲料的饲粮，分别为苜蓿组（23%苜蓿干草+7%羊草，AH组）、玉米秸秆组（30%玉米秸秆，CS组）和稻草组（30%稻草，RS组），饲粮精粗比均为55:45。试验期12周。结果表明:CS组和RS组奶牛的粪氮排泄量显著高于AH组（P<0.05），RS组奶牛的尿氮排泄量显著高于AH组和CS组（P<0.05）。RS组奶牛的血清尿素氮和肌酸酐含量显著高于AH组（P<0.05）。RS组奶牛的血清总胆固醇含量显著高于AH组和CS组（P<0.05）。CS组奶牛的血清谷丙转氨酶活性显著高于AH组（P<0.05），而血清谷草转氨酶活性显著高于AH组和RS组（P<0.05）。各组奶牛的肝脏、肾脏组织切片结构清晰，未见明显异常。由此可见，饲喂玉米秸秆或稻草粗饲料的奶牛粪氮、尿氮排泄量的增加导致奶牛氮的利用效率降低。各组奶牛肝脏和肾脏的组织形态无显著变化，但饲喂玉米秸秆或稻草饲粮可导致奶牛血清中反映肝脏、肾脏健康功能的指标升高。

关键词： 奶牛; 氮代谢; 肝脏; 血清

本文引用格式

王炳, 蒋林树, 刘建新 . 稻草或玉米秸秆替代苜蓿对奶牛氮代谢、血清生理生化指标及肝脏和肾脏组织形态变化的影响[J]. 动物营养学报, 2017 , 29(6) : 1921 -1929 . DOI: 10.3969/j.issn.1006-267x.2017.06.013

Abstract

The aim of this study was to investigate the effects of alfalfa replacement by rice straw or corn stover on nitrogen metabolism, serum physiological-biochemical indices and histomorphology of liver and kidney of dairy cows. Forty-five multiparous Holstein dairy cows were blocked and randomly assigned to 3 groups with 15 cows per group. Cows in the 3 groups were fed with different roughages:alfalfa group (23% alfalfa hay and 7% Chinese wild rye hay, AH group), corn stover group (30% corn stover, CS group) and rice straw group (30% rice straw, RS group), dietary forage to concentrate ratio was 55:45. The experiment lasted for 12 weeks. The results showed that the fecal nitrogen excretion of cows in CS group and RS group was significanly higher than that in AH group (P<0.05), the urinary nitrogen excretion of cows in RS group was significanly higher than that in AH group and CS group (P<0.05). The serum urea nitrogen and creatinine contents of cows in RS group were significanly higher than those in AH group (P<0.05). The serum total cholesterol content in RS group was significanly higher than that in AH group and CS group (P<0.05). The serum alanine aminotransferase activity in CS group was significanly higher than that in AH (P<0.05), while the serum glutamic oxaloacetic transaminase activity was significanly higher than that in AH group and RS group (P<0.05). The histological structure of liver and kidney in each group was clear, and no obvious abnormality was found. From above, the greater excretion of fecal nitrogen and urinary nitrogen of cows fed rice straw or corn stover resulte lower nitrogen conversion efficiency to milk protein of cows. No apparent morphological changes in liver and kidney are observed. However, the serum indexes responding the healthy function of liver and kidney are increased when cows fed with corn stover or rice straw.

Key words： dairy cows; nitrogen metabolism; liver; serum

参考文献

[1] 王炳.饲喂秸秆日粮奶牛泌乳性能低下的消化吸收与代谢机制研究[D].博士学位论文.杭州:浙江大学,2016:11-12.

[2] 钟华平,岳燕珍,樊江文.中国作物秸秆资源及其利用[J].资源科学,2003,25(4):62-67.

[3] PANG Y Z,LIU Y P,LI X J,et al.Improving biodegradability and biogas production of corn stover through sodium hydroxide solid state pretreatment[J].Energy & Fuels,2008,22(4):2761-2766.  

[4] 毕于运,高春雨,王亚静,等.中国秸秆资源数量估算[J].农业工程学报,2009,25(12):211-217.

[5] KEBEDE G.Effect of urea-treatment and Leucaena (Leucaena leucocephala) supplementation on the utilization of wheat straw as feed for sheep[D].Master's Thesis.Haramaya,Ethiopia:Haramaya University,2006:66.

[6] ZHAO T Z,LI H Y.Study on ruminal degradation of mainly protein and fiber sources in dairy diets[J].Contemporary Animal Husbandary,2009,11:29-32.

[7] WANG B,MAO S Y,YANG H J,et al.Effects of alfalfa and cereal straw as a forage source on nutrient digestibility and lactation performance in lactating dairy cows[J].Journal of Dairy Science,2014,97(12):7706-7715.  

[8] WANG B,SUN H Z,XU N N,et al.Amino acid utilization of lactating dairy cows when diets are changed from an alfalfa-based diet to cereal straw-based diets[J].Animal Feed Science and Technology,2016,217:56-66.

[9] WANG B,ZHAO F Q,ZHANG B X,et al.An insufficient glucose supply causes reduced lactose synthesis in lactating dairy cows fed rice straw instead of alfalfa hay[J].Journal of Animal Science,2016,94(11):4771-4780.  

[10] LAPORTE M F,PAQUIN P.Near-infrared analysis of fat,protein,and casein in cow's milk[J].Journal of Agricultural and Food Chemistry,1999,47(7):2600-2605.  

[11] AOAC.Official methods of analysis[J].14th ed.Arlington,VA:Association of Official Analytical Chemists,1990.

[12] LEE C,HRISTOV A N.Short communication:evaluation of acid-insoluble ash and indigestible neutral detergent fiber as total-tract digestibility markers in dairy cows fed corn silage-based diets[J].Journal of Dairy Science,2013,96(8):5295-5299.  

[13] Statistical Analysis Systems Institute.SAS/STAT User's Guide:Statistics.Version 8.1[Z].2000.

[14] WOLFE A H,PATZ J A.Reactive nitrogen and human health:acute and long-term implications[J].AMBIO:A Journal of the Human Environment,2002,31(2):120-125.  

[15] KAUFFMAN A J,ST-PIERRE N R.The relationship of milk urea nitrogen to urine nitrogen excretion in Holstein and Jersey cows[J].Journal of Dairy Science,2001,84(10):2284-2294.  

[16] ZHU W,FU Y,WANG B,et al.Effects of dietary forage sources on rumen microbial protein synthesis and milk performance in early lactating dairy cows[J].Journal of Dairy Science,2013,96(3):1727-1734.  

[17] HUHTANEN P,CABEZAS-GARCIA E H,KRIZSAN S J,et al.Evaluation of between-cow variation in milk urea and rumen ammonia nitrogen concentrations and the association with nitrogen utilization and diet digestibility in lactating cows[J].Journal of Dairy Science,2015,98(5):3182-3196.  

[18] MAHRT A,BURFEIND O,HEUWIESER W.Effects of time and sampling location on concentrations of β-hydroxybutyric acid in dairy cows[J].Journal of Dairy Science,2014,97(1):291-298.  

[19] GRVNWALDT E G,GUEVARA J C,ESTÉVEZ O R,et al.Biochemical and haematological measurements in beef cattle in Mendoza plain rangelands (Argentina)[J].Tropical Animal Health and Production,2005,37(6):527-540.  

[20] COZZI G,RAVAROTTO L,GOTTARDO F,et al.Short communication:reference values for blood parameters in Holstein dairy cows:effects of parity,stage of lactation,and season of production[J].Journal of Dairy Science,2011,94(8):3895-3901.  

[21] MILLER N E,HAMMETT F,SALTISSI S,et al.Relation of angiographically defined coronary artery disease to plasma lipoprotein subfractions and apolipoproteins[J].British Medical Journal,1981,282(6278):1741-1744.  

[22] PADMAJA K,RAO D S T.Clinical,biochemical and therapeutic responses of Laurabolin and Ketonex to post parturient indigestion (PPI) related subclinical ketosis in buffalo[J].Animal Science Reporter,2013,7(1):35-40.

[23] BERTONI G,TREVISI E.Use of the liver activity index and other metabolic variables in the assessment of metabolic health in dairy herds[J].The Veterinary Clinics of North America:Food Animal Practice,2013,29(2):413-431.  

[24] ABDELQADER M M,HIPPEN A R,KALSCHEUR K F,et al.Isolipidic additions of fat from corn germ,corn distillers grains,or corn oil in dairy cow diets[J].Journal of Dairy Science,2009,92(11):5523-5533.  

[25] SORDILLO L M,RAPHAEL W.Significance of metabolic stress,lipid mobilization,and inflammation on transition cow disorders[J].Veterinary Clinics of North America:Food Animal Practice,2013,29(2):267-278.  

[26] GRAUGNARD D E,MOYES K M,TREVISI E,et al.Liver lipid content and inflammometabolic indices in peripartal dairy cows are altered in response to prepartal energy intake and postpartal intramammary inflammatory challenge[J].Journal of Dairy Science,2013,96(2):918-935.  

[27] MILES E D,MCBRIDE B W,JIA Y,et al.Glutamine synthetase and alanine transaminase expression are decreased in livers of aged vs. young beef cows and GS can be upregulated by 17β-estradiol implants[J].Journal of Animal Science,2015,93(9):4500-4509.  

[28] ARIAS A,GARCIA-VILLORIA J,RIBES A.Guanidinoacetate and creatine/creatinine levels in controls and patients with urea cycle defects[J].Molecular Genetics and Metabolism,2004,82(3):220-223.  

[29] NUDDA A,CORREDDU F,MARZANO A,et al.Effects of diets containing grape seed,linseed,or both on milk production traits,liver and kidney activities,and immunity of lactating dairy ewes[J].Journal of Dairy Science,2015,98(2):1157-1166.  

[30] ABDEL-WAHHAB M A,NADA S A,KHALIL F A.Physiological and toxicological responses in rats fed aflatoxin-contaminated diet with or without sorbent materials[J].Animal Feed Science and Technology,2002,97(3/4):209-219.

[31] BERNARDINI D,SEGATO S,MARCHESINI G,et al.Changes in the metabolic profile and performance of dairy cows fed two dietary crude protein concentrations[M]//BOITI C,FERLAZZO A,GAITI A,et al.Trends in Veterinary Sciences.Berlin Heidelberg:Springer,2013:125-128.

[32] IQBAL M,SHARMA S D,REZAZADEH H,et al.Glutathione metabolizing enzymes and oxidative stress in ferric nitrilotriacetate mediated hepatic injury[J].Redox Report,1996,2(6):385-391.  

[33] WANG C,LIU H Y,WANG Y M,et al.Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows[J].Journal of Dairy Science,2010,93(8):3661-3670.  

[34] APELO S I A,KNAPP J R,HANIGAN M D.Invited review:current representation and future trends of predicting amino acid utilization in the lactating dairy cow[J].Journal of Dairy Science,2014,97(7):4000-4017.  
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