水产营养 Aquaculture Nutrition

豆油部分或全部替代饲料中鱼油对中华条颈龟肠道形态及菌群结构的影响

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  • 1. 海南师范大学生命科学学院, 海口 571158;
    2. 中国科学院成都生物研究所, 成都 610041
丁利(1982-),女,河南夏邑人,副教授,博士,从事动物学研究。E-mail:dingli705@163.com

收稿日期: 2016-12-01

  网络出版日期: 2017-06-07

基金资助

国家自然科学基金项目(31360642,31372228);海南省大学生创新训练项目(3010151072)

Effects of Partial or Total Replacement of Fish Oil by Soybean Oil on Intestinal Morphology and Microbiota Structure of Chinese Striped-Neck Turtle (Mauremys sinensis)

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  • 1. College of Life Science, Hainan Normal University, Haikou 571158, China;
    2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China

Received date: 2016-12-01

  Online published: 2017-06-07

摘要

本试验通过组织学染色及16S rRNA V3~V4区测序研究豆油部分或全部替代饲料中鱼油对中华条颈龟(Mauremys sinensis)肠道形态及菌群结构的影响。选取中华条颈龟雌龟[4龄,平均体重(1 557±307) g]24只,随机分为4组,每组6只。Ⅰ组(对照组)饲喂含1%鱼油的配合饲料(全鱼油饲料,豆油与鱼油的比例为0:3),3个试验组分别饲喂用豆油替代全鱼油饲料中33%(Ⅱ组,豆油与鱼油的比例为1:2)、67%(Ⅲ组,豆油与鱼油的比例为2:1)和100%(Ⅳ组,豆油与鱼油的比例为3:0)鱼油的饲料。每周喂食2次,试验期为10个月。结果表明:1)Ⅰ和Ⅲ组十二指肠绒毛长/隐窝深度(VH/CD)显著高于Ⅱ和Ⅳ组(P<0.05)。2)根据序列相似性97%水平划分,Ⅰ、Ⅱ、Ⅲ和Ⅳ组操作分类单元(OTU)总数分别为396、321、347和331个,特有OTU个数分别为43、3、4和5个,分别被鉴定出139、117、128、120个属。十二肠道优势菌门主要包含拟杆菌门(Bacteroidetes)、厚壁杆菌门(Firmicutes)、梭杆菌门(Fusobacteria)和变形菌门(Proteobacteria)4个门。Ⅱ、Ⅲ和Ⅳ组梭杆菌门和变形菌门所占比例增加,拟杆菌门和厚壁菌门所占比例下降,Ⅲ组厚壁杆菌门和拟杆菌门所占比例与Ⅰ组差距最小。细菌Ace指数和香农指数表现为Ⅰ组最高,其次是Ⅲ和Ⅳ组,Ⅱ组最小;细菌辛普森指数表现为Ⅱ组最大,其次是Ⅲ和Ⅳ组,Ⅰ组最小。由此得出,饲喂用豆油替代67%鱼油的饲料的中华条颈龟的肠道细胞发育成熟度以及菌群结构与饲喂全鱼油饲料的中华条颈龟相近。

本文引用格式

丁利, 李江月, 薛珊, 史海涛, 洪美玲 . 豆油部分或全部替代饲料中鱼油对中华条颈龟肠道形态及菌群结构的影响[J]. 动物营养学报, 2017 , 29(6) : 1940 -1948 . DOI: 10.3969/j.issn.1006-267x.2017.06.015

Abstract

This study was conducted to investigate the effects of partial or total replacement of fish oil by soybean oil on intestinal morphology and microbiota structure of Chinese striped-neck turtle (Mauremys sinensis) by histological staining and 16S rRNA V3-V4 region sequencing. Twenty 4-year adult female Chinese striped-neck turtles[4-year-old and the initial body weight of (1 557±307) g] were randomly divided into 4 groups and each group had 6 turtles. Turtles in group Ⅰ (control group) were fed a formula feed containing 1% fish oil which was a whole fish oil diet with the ratio of soybean oil to fish oil was 0:3, and others in three experimental groups were fed diets used soybean oil to replace 33% (groupⅡ), 67% (group Ⅲ) and 100% (group Ⅳ) fish oil based on the whole fish oil diet and the ratios of soybean oil to fish oil of them were 1:2, 2:1 and 3:0, respectively. Turtles were fed 2 times per week, and the experiment lasted for 10 months. The results showed as follows:1) the ratio of villus height to crypt depth (VH/CD) of duodenum in groups Ⅰ and Ⅲ was significantly higher than that in groups Ⅱ and Ⅳ (P<0.05). 2) According to the 97% level of sequence similarity, the total numbers of operational taxonomic unit (OTU) in groupsⅠ, Ⅱ, Ⅲ and Ⅳ were 396, 321, 347 and 331, while the specific OTU numbers were 43, 3, 4 and 5, and 139, 117, 128, 120 genus were identified, respectively. The dominant phyla in the duodenum were Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria. The proportions of Fusobacteria and Proteobacteriai were increased while the proportions of Bacteroidetes and Firmicutes were decreased in groups Ⅱ, Ⅲ and Ⅳ. However, the small gap of Firmicutes and Bacteroidetes between groups Ⅰ and Ⅲ. The bacterial Ace index and Shanno index were the highest in group Ⅰ, the middle in groups Ⅲ and Ⅳ, and the lowest in group Ⅱ. The bacterial Simpson index was the highest in group Ⅱ, the middle in groups Ⅲ and Ⅳ, and the lowest in group Ⅰ. Therefore, the development maturation degree of intestinal cells and intestinal microbiota structure of Chinese striped-neck turtle fed the diet with 67% fish oil replacement by soybean oil are similar to Chinese striped-neck turtle fed the whole fish oil diet.

参考文献

[1] ECKBURG P B,BIK E M,BERNSTEIN C N,et al.Diversity of the human intestinal microbial flora[J].Science,2005,308(5728):1635-1638.  

[2] QIN J J,LI R Q,RAES J,et al.A human gut microbial gene catalogue established by metagenomic sequencing[J].Nature,2010,464(7285):59-65.  

[3] SOCCINI C,FERRI V.Bacteriological screening of Trachemys scripta elegans and Emys orbicularis in the Po plain (Italy)[J].Biologia,Bratislava,2004,59(Suppl.14):201-207.

[4] 杜爽,张文飞,史海涛.基于16S rRNA序列分析红耳龟肠道拟杆菌和厚壁杆菌菌群多样性[J].基因组学与应用生物学,2013,32(6):700-706.

[5] 陈杏云,曾本华,魏泓,等.高脂饮食对菌群人源化小鼠肠道菌群结构的影响[J].食品科学,2013,34(17):278-283.

[6] 曹宏芳,张家超,王芳,等.高脂饮食对雄性SD大鼠肠道菌群的影响[J].中国微生态学杂志,2012,24(2):102-108.

[7] 刘雪姬,陈庆森,闫亚丽.高脂饮食对小鼠肠道菌群的影响[J].食品科学,2011,32(23):306-311.

[8] ZHANG C H,ZHANG M H,PANG X Y,et al.Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations[J].The ISME Journal,2012,6(10):1848-1857.  

[9] ZHANG M L,SUN Y H,CHEN K,et al.Characterization of the intestinal microbiota in Pacific white shrimp,Litopenaeus vannamei,fed diets with different lipid sources[J].Aquaculture,2014,434:449-455.

[10] DU X,XU X Q,TIAN Y,et al.Morphology and microbiota of different section of intestines and their influences induced by dietary fish oil in Shan partridge ducks (Anas platyrhynchos)[J].Journal of Agricultural Biotechnology,2016,24(11):1652-1663.

[11] LIU T Y,HOUGEN H,VOLLMER A C,et al.Gut bacteria profiles of Mus musculus at the phylum and family levels are influenced by saturation of dietary fatty acids[J].Anaerobe,2012,18(3):331-337.  

[12] PICKOVA J,MØRKØRE T.Alternate oils in fish feeds[J].European Journal of Lipid Science and Technology,2007,109(3):256-263.  

[13] 裘正元,盛成,洪美玲,等.豆油替代鱼油对中华条颈龟血脂代谢及相关酶活的影响[J].水产科学,2015,34(7):453-458.

[14] COSTELLO E K,GORDON J I,SECOR S M,et al.Postprandial remodeling of the gut microbiota in Burmese pythons[J].The ISME Journal,2010,4(11):1375-1385.  

[15] HONG P Y,WHEELER E,CANN I K O,et al.Phylogenetic analysis of the fecal microbial community in herbivorous land and marine iguanas of the Galàpagos Islands using 16S rRNA-based pyrosequencing[J].The ISME Journal,2011,5(9):1461-1470.  

[16] MACKIE R I,NELSON D M,WHEELER E,et al.Fermentative digestion in herbivorous lizards:bacterial population analysis in the intestinal tract of free-living land (Conolophus pallidus) and marine iguanas (Amblyrhynchus cristatus) on the Galapagos archipelago[M]//MORRIS S,VOSLOO A.Molecules to migration:the pressures of life.Bologna:Medimond Publishing Company,2008:193-202.

[17] 刘秋东,张中文,刘凤华,等.复方白头翁胶囊对腹泻犬小肠绒毛长度和隐窝深度的影响[J].北京农学院学报,2011,26(3):38-40.

[18] 刘晓静,史彬林,赵育国,等.日粮中添加沙蒿籽粉对肉仔鸡肠绒毛形态及肠道菌群的影响[J].饲料工业,2011,32(5):13-15.

[19] ZEITZ J,FENNHOFF J,KLUGE H,et al.Effects of dietary fats rich in lauric and myristic acid on performance,intestinal morphology,gut microbes,and meat quality in broilers[J].Poultry Science,2015,94(10):2404-2413.  

[20] 陈丛亮,杨洪勋,杨磊,等.半胱胺提高畜禽营养物质消化、吸收和利用率的作用机制探讨[J].饲料研究,2008(12):18-21.

[21] NGOC T T B,HONG T T T,LEN N T,et al.Effect of fibre level and fibre source on gut morphology and micro-environment in local (Mong Cai) and exotic (Landrace×Yorkshire) pigs[J].Asian-Australasian Journal of Animal Sciences,2012,25(12):1726-1733.  

[22] CHEN H,MAO X B,CHE L Q,et al.Impact of fiber types on gut microbiota,gut environment and gut function in fattening pigs[J].Animal Feed Science and Technology,2014,195(9):101-111.

[23] GABRIEL I,LESSIRE M,MALLET S,et al.Microflora of the digestive tract:critical factors and consequences for poultry[J].World's Poultry Science Journal,2006,62(3):499-511.

[24] OSBORN O,OLEFSKY J M.The cellular and signaling networks linking the immune system and metabolism in disease[J].Nature Medicine,2012,18(3):363-374.  
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