动物营养学报 >

2017 , Vol. 29 >Issue 6: 2179 - 2187

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.06.041

研究简报 Short communications

湿热应激对藏绵羊和山羊生长性能、抗氧化能力以及免疫功能的影响

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  • 四川农业大学动物营养研究所, 雅安 625014
张灿(1992-),男,新疆呼图壁人,硕士研究生,从事反刍动物营养研究。E-mail:316383773@qq.com

收稿日期: 2016-12-14

  网络出版日期: 2017-06-07

基金资助

青藏高原社区畜牧业行业科技项目（201203008）

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Effects of Moist-Heat Stress on Growth Performance, Oxidation Resistance and Immunity of Tibetan Sheep and Goats

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  • Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2016-12-14

  Online published: 2017-06-07

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摘要

本试验旨在研究藏绵羊和山羊在相同营养及湿热应激条件下血清激素、抗氧化和免疫指标的变化规律，考察湿热应激对藏绵羊和山羊生长性能、抗氧化能力以及免疫功能影响的差异。选取年龄和体重[（45.83±3.54） kg]相近的藏绵羊与山羊（波尔山羊×本地黄羊）各6只，试验共进行135 d，其中预试期15 d，正试期120 d。每日测定温湿度指数（THI），每月测定藏绵羊和山羊的血清相关指标。结果表明:1）5-7月份，羊舍THI随月份的增加显著升高（P<0.05），6-8月份羊舍THI均大于72，因此将6-8月份定为湿热应激期。2）7、8月份藏绵羊和山羊直肠温度和呼吸频率均显著高于5月份（P<0.05），且湿热应激期内藏绵羊直肠温度和呼吸频率均显著高于山羊（P<0.05）。3）正试期内，湿热应激使得藏绵羊和山羊的干物质采食量最大降幅分别为10.70%和10.44%，平均日增重（ADG）最大降幅分别为50.00%和47.82%。4）羊舍THI由71.17（5月份）升高到76.82（7月份），藏绵羊和山羊血清皮质醇和胰岛素浓度显著升高（P<0.05），血清葡萄糖和三碘甲状腺原氨酸浓度显著降低（P<0.05）。湿热应激下，藏绵羊血清中生长激素和甲状腺素浓度的最大降幅均大于山羊。5）除藏绵羊7月份和山羊8月份血清总抗氧化能力，藏绵羊和山羊7、8月份的血清超氧化物歧化酶、谷胱甘肽过氧化物酶活性和总抗氧化能力均显著低于5月份（P<0.05）外，血清丙二醛浓度均显著高于5月份（P<0.05）。湿热应激下，山羊血清超氧化物歧化酶活性、总抗氧化能力和丙二醛浓度变化幅度均大于藏绵羊。6）与5月份相比，藏绵羊和山羊8月份的血清免疫球蛋白A、免疫球蛋白M、免疫球蛋白G和白细胞介素-2浓度显著降低（P<0.05），而血清肿瘤坏死因子-α浓度则显著升高（P<0.05）。湿热应激下，藏绵羊血清免疫球蛋白、白细胞介素-2和肿瘤坏死因子-α浓度的变化幅度均大于山羊。综上所述，湿热应激状态下藏绵羊和山羊的呼吸频率和直肠温度升高，抗氧化能力和免疫功能降低，从而导致生长性能降低。藏绵羊生长性能和免疫功能受湿热应激影响较大，而山羊抗氧化能力受湿热应激影响较大。

关键词： 湿热应激; 藏绵羊; 山羊; 生长性能; 抗氧化能力; 免疫功能

本文引用格式

张灿, 王之盛, 彭全辉, 邹华围, 景小平, 蒲启建 . 湿热应激对藏绵羊和山羊生长性能、抗氧化能力以及免疫功能的影响[J]. 动物营养学报, 2017 , 29(6) : 2179 -2187 . DOI: 10.3969/j.issn.1006-267x.2017.06.041

Abstract

The present study was conducted to investigate the variation regularities of serum hormone, antioxidant and immune indices of Tibetan sheep and goats raised under the same nutritional condition with moist-heat environment, and aimed to reveal the different effects of moist-heat stress on growth performance, oxidation resistance and immunity between Tibetan sheep and goats. Six individuals of Tibetan sheep and goats (Boar goat×local yellow goat) were selected based on similar age and body weight[(45.83±3.54) kg]. Feeding experiment lasted for 135 days, including a 15-day adaption period and another 120-day text period. Temperature and humidity of sheep barn were measured daily and temperature-humidity index (THI) was calculated, and serum related indices of Tibetan sheep and goats were monitored monthly. The results showed as follows:1) the THI of sheep barn was significantly raised in May to July along with month increasing (P<0.05). The THI of sheep barn was above 72 in June to August so that this period was regarded as the moist-heat stress period. 2) Respiratory rate and rectal temperature of Tibetan sheep and goats were all significantly increased in July and August compared with those in May (P<0.05). Compared with goats, Tibetan sheep presented a significantly higher respiratory rate and rectal temperature during the moist-heat stress period (P<0.05). 3) During the text period, the maximal decrease of dry matter intake (DMI) of Tibetan sheep and goats was 10.70% and 10.44%, respectively, besided, the maximal decrease of average daily gain was 50.00% and 47.82%, respectively. 4) The THI of sheep barn was raised from 71.17 (May) to 76.82 (July) resulted in a significant rise in serum cortisol and insulin concentrations (P<0.05) accompanied with a significant reduction in serum glucose and triiodothyronine concentrations (P<0.05). The maximal amplifications in serum growth hormone and thyroxine concentrations of Tibetan sheep were larger than those of goats under moist-heat stress. 5) In addition to the serum total antioxidant capacity (T-AOC) of Tibetan sheep (July) and goats (August), the serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and T-AOC in July and August were all significantly decreased compared with those in May (P<0.05), while the serum malondialdehyde (MDA) concentration was significantly increased (P<0.05). Under moist-heat stress, the variation ranges of serum SOD activity, T-AOC and MDA concentration of goats were larger than those of Tibetan sheep. 6) The serum immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG) and interleukin-2 (IL-2) concentrations of Tibetan sheep and goats were all significantly decreased in August compared with those in May (P<0.05), while the serum tumor necrosis factor-α (TNF-α) concentration was significantly increased (P<0.05). Under moist-heat stress, the variation ranges of serum immunoglobulin, IL-2 and TNF-α concentrations of Tibetan sheep were larger than those of goats. It is concluded that moist-heat stress increase the respiratory rate and rectal temperature in both Tibetan sheep and goats accompanied with the decrease of growth performance, oxidation resistance and immunity. The Tibetan sheep is affected severer by moist-heat stress on growth performance and immunity, whereas the oxidation resistance of goat is affected severer by moist-heat stress.

Key words： moist-heat stress; Tibetan sheep; goat; growth performance; oxidation resistance; immunity

参考文献

[1] 中华人民共和国国家统计局.中国统计年鉴[M].北京:中国统计出版社,2014.

[2] RHOADS M L,RHOADS R P,VANBAALE M J,et al.Effects of heat stress and plane of nutrition on lactating Holstein cows:Ⅰ.Production,metabolism,and aspects of circulating somatotropin[J].Journal of Dairy Science,2009,92(5):1986-1997.  

[3] MCMANUS C,PRESCOTT E,PALUDO G R,et al.Heat tolerance in naturalized Brazilian cattle breeds[J].Livestock Science,2009,120(3):256-264.  

[4] GAUGHAN J,LACETERA N,VALTORTA S E,et al.Response of domestic animals to climate challenges[M]//EBI K L,BURTON I,MCGREGOR G R.Biometeorology for adaptation to climate variability and change.Netherlands:Springer,2009:131-170.

[5] 杨游,袁志琳,董国忠,等.饲粮中添加脂肪酸钙和烟酸铬对热应激奶牛产奶性能、生理指标及血清生化指标的影响[J].动物营养学报,2012,24(1):145-151.

[6] 杨耐德,黄晓亮,高振华,等.烟酸对热应激奶牛营养物质表观消化率及血清生化指标的影响[J].中国饲料,2010(12):20-23.

[7] MAUST L E,MCDOWELL R E,HOOVEN N W.Effect of summer weather on performance of holstein cows in three stages of lactation[J].Journal of Dairy Science,1972,55(8):1133-1139.  

[8] JOHNSON J S,SCHARF B,WEABER R L,et al.Patterns of heat response and adaptation on summer pasture:a comparison of heat-sensitive (Angus) and-tolerant (Romosinuano) cattle[J].Journal of Thermal Biology,2012,37(1):344-350.

[9] SILANIKOVE N.Effects of heat stress on the welfare of extensively managed domestic ruminants[J].Livestock Production Science,2000,67(1/2):1-18.

[10] SRIKANDAKUMAR A,JOHNSON E H,MAHGOUB O.Effect of heat stress on respiratory rate,rectal temperature and blood chemistry in omani and australian merino sheep[J].Small Ruminant Research,2003,49(2):193-198.  

[11] MAHJOUBI E,YAZDI M H,AGHAZIARATI N,et al.The effect of cyclical and severe heat stress on growth performance and metabolism in Afshari lambs[J].Journal of Animal Science,2015,93(4):1632-1640.  

[12] MAHJOUBI E,AMANLOU H,MIRZAEI-ALAMOUTI H R,et al.The effect of cyclical and mild heat stress on productivity and metabolism in Afshari lambs[J].Journal of Animal Science,2014,92(3):1007-1014.  

[13] RONCHI B,STRADAIOLI G,SUPPLIZI A V,et al.Influence of heat stress or feed restriction on plasma progesterone,oestradiol-17β,LH,FSH,prolactin and cortisol in Holstein heifers[J].Livestock Production Science,2001,68(2/3):231-241.

[14] RENAVILLE R,HAMMADI M,PORTETELLE D.Role of the somatotropic axis in the mammalian metabolism[J].Domestic Animal Endocrinology,2002,23(1/2):351-360.

[15] MAGDUB A,JOHNSON H D,BELYEA R L.Effect of environmental heat and dietary fiber on thyroid physiology of lactating cows[J].Journal of Dairy Science,1982,65(12):2323-2331.  

[16] MCGUIRE M A,BEEDE D K,COLLIER R J,et al.Effects of acute thermal stress and amount of feed intake on concentrations of somatotropin,insulin-like growth factor (IGF)-Ⅰ and IGF-Ⅱ,and thyroid hormones in plasma of lactating Holstein cows[J].Journal of Animal Science,1991,69(5):2050-2056.  

[17] O'BRIEN M D,RHOADS R P,SANDERS S R,et al.Metabolic adaptations to heat stress in growing cattle[J].Domestic Animal Endocrinology,2010,38(2):86-94.  

[18] WHEELOCK J B,RHOADS R P,VANBAALE M J,et al.Effects of heat stress on energetic metabolism in lactating Holstein cows[J].Journal of Dairy Science,2010,93(2):644-655.  

[19] KURATA M,SUZUKI M,AGAR N S.Antioxidant systems and erythrocyte life-span in mammals[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1993,106(3):477-487.  

[20] MILLER J K,BRZEZINSKA-SLEBODZINSKA E,MADSEN F C.Oxidative stress,antioxidants,and animal function[J].Journal of Dairy Science,1993,76(9):2812-2823.  

[21] HARMON R J,LU M,TRAMMEL D S,et al.Influence of heat stress and calving on antioxidant activity in bovine blood[J].Journal of Dairy Science,1997,80(2):264-272.  

[22] BERNABUCCI U,RONCHI B,LACETERA N,et al.Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season[J].Journal of Dairy Science,2002,85(9):2173-2179.  

[23] MEGAHED G A,ANWAR M M,WASFY S I,et al.Influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (Bubalus bubalis):thermo-protective role of antioxidant treatment[J].Reproduction in Domestic Animals,2008,43(6):672-677.  

[24] YANG L,TAN G Y,FU Y Q,et al.Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration,ROS production and lipid peroxidation in broiler chickens[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2010,151(2):204-208.  

[25] CAROPRESE M,MARZANO A,ENTRICAN G,et al.Immune response of cows fed polyunsaturated fatty acids under high ambient temperatures[J].Journal of Dairy Science,2009,92(6):2796-2803.  

[26] TAO S,MONTEIRO A P A,THOMPSON I M,et al.Effect of late-gestation maternal heat stress on growth and immune function of dairy calves[J].Journal of Dairy Science,2012,95(12):7128-7136.  

[27] STARKIE R,HARGREAVES M,ROLLAND J,et al.Heat stress,cytokines,and the immune response to exercise[J].Brain,Behavior,and Immunity,2005,19(5):404-412.  

[28] CHENG J B,ZHENG N,SUN X Z,et al.Feeding rumen-protected gamma-aminobutyric acid enhances the immune response and antioxidant status of heat-stressed lactating dairy cows[J].Journal of Thermal Biology,2016,60:103-108.
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