动物营养学报 >

2017 , Vol. 29 >Issue 9: 3091 - 3098

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.010

禽营养 Poultry nutrition

蛋氨酸缺乏对蛋鸡产蛋后期生产性能、血清游离氨基酸含量和肝脏蛋氨酸代谢相关基因表达的影响

展开
  • 1. 河南科技大学动物科技学院, 河南省饲草饲料资源开发与畜禽健康养殖院士工作站, 洛阳 471003;
    2. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术研究中心, 长沙 410125
刘怡琳(1992-),女,河南南阳人,硕士研究生,从事动物营养与饲料科学研究。E-mail:15236286951@163.com

收稿日期: 2017-02-13

  网络出版日期: 2017-09-08

基金资助

国家重点研发计划（2016YFD0500504）

收起

Effects of Methionine Deficiency on Performance, Serum Free Amino Acid Contents and Liver Methionine Metabolism Gene Expression of Laying Hens during Late Period of Laying

Expand
  • 1. Henan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
    2. Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2017-02-13

  Online published: 2017-09-08

Fold

摘要

本试验旨在研究蛋氨酸缺乏对蛋鸡产蛋后期生产性能、血清游离氨基酸含量和肝脏蛋氨酸代谢相关酶基因表达的影响。选取180只62周龄的海兰灰蛋鸡，根据产蛋率均匀分成3组（每组6个重复，每个重复10只）：对照组饲喂蛋氨酸水平为0.33%的饲粮，蛋氨酸缺乏组分别饲喂蛋氨酸水平为0.21%和0.27%的饲粮，试验期90 d。结果表明：1）饲粮蛋氨酸缺乏显著降低了蛋鸡的平均日采食量、产蛋率、平均日产蛋重、平均蛋重和不合格蛋率（P<0.05），显著提高了料蛋比（P<0.05）。2）蛋氨酸缺乏显著降低蛋鸡血清中蛋氨酸的含量（P<0.05）。0.21%蛋氨酸组蛋鸡血清中丝氨酸、甘氨酸和丙氨酸的含量显著高于其他2组（P<0.05）。0.27%蛋氨酸组蛋鸡血清中缬氨酸、异亮氨酸和精氨酸的含量显著低于对照组（P<0.05），脯氨酸的含量显著低于0.21%蛋氨酸组（P<0.05）。3）与对照组相比，0.21%蛋氨酸组蛋鸡肝脏中DNA甲基转移酶1、N6-甲基腺苷（m6A）甲基转移酶3（METTL3）和m6A甲基转移酶14的表达量显著提高（P<0.05），0.27%蛋氨酸组蛋鸡肝脏中METTL3的表达量显著提高（P<0.05）。4）与对照组相比，0.21%蛋氨酸组蛋鸡肝脏中甲硫氨酸腺苷转移酶1a、5-甲基四氢叶酸-同型半胱氨酸甲基转移酶（MTR）和胱硫醚-β-合成酶的表达量显著提高（P<0.05），0.27%蛋氨酸组蛋鸡肝脏中MTR的表达量显著提高（P<0.05）。蛋氨酸缺乏对蛋鸡肝脏甲硫氨酸腺苷高半胱氨酸酶和甜菜碱高半胱氨酸甲基转移酶的表达量无显著影响（P>0.05）。由以上结果可知：蛋鸡饲粮高水平的蛋氨酸缺乏会降低蛋鸡的生产性能，可能与蛋氨酸缺乏改变蛋氨酸代谢途径，影响DNA和RNA的甲基化过程有关。

关键词： 蛋氨酸; 蛋鸡; 生产性能; 蛋氨酸代谢; 甲基化; 基因表达

本文引用格式

刘怡琳, 吴信, 印遇龙, 王占彬 . 蛋氨酸缺乏对蛋鸡产蛋后期生产性能、血清游离氨基酸含量和肝脏蛋氨酸代谢相关基因表达的影响[J]. 动物营养学报, 2017 , 29(9) : 3091 -3098 . DOI: 10.3969/j.issn.1006-267x.2017.09.010

Abstract

This experiment was conducted to study the effects of methionine deficiency on performance, serum free amino acid contents and liver methionine metabolism gene expression of laying hens during late period of laying. Based on the egg production, a total of 180 Hy-Line Grey laying hens at 62 weeks of age were allocated to 3 groups with 6 replicates each and 10 hens in each replicate. Hens in three groups were fed different diets which contained 0.21%, 0.27% and 0.33% (control group) methionine, respectively, and the experiment period was 90 days. The results showed as follows:1) dietary methionine deficiency significantly reduced the average daily feed intake, laying rate, average daily egg production, average egg weight and unqualified rate of eggs (P<0.05), but significantly increased the ratio of feed to egg of laying hens (P<0.05). 2) Methionine deficiency significantly reduced the serum methionine content of laying hens (P<0.05). The contents of serum serine, glycine and alanine in 0.21% methionine group were significantly higher than those in the other two groups (P<0.05). The contents of serum valine, isoleucine and arginine in 0.27% methionine group were significantly lower than those in control group (P<0.05), and serum proline content in 0.27% methionine group was significantly lower than that in 0.21% methionine group (P<0.05). 3) Compared with control group, the expression levels of DNA methyltransferase 1, N6-adenosine-methyltransferase subunit 3 (METTL3) and N6-adenosine-methyltransferase subunit 14 in liver of hens in 0.21% methionine group were significantly improved (P<0.05), and the METTL3 expression level in liver of hens in 0.27% methionine group was also significantly improved (P<0.05). 4) Compared with control group, the expression levels of methionine adenosyltransferase 1a, cystathionine-beta-synthase and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) in liver of hens in 0.21% methionine group were significantly improved (P<0.05), and the MTR expression level in liver of hens in 0.27% methionine group was also significantly improved (P<0.05). Methionine deficiency had no significant influence on the expression levels of adenosylhomocysteinas and betaine homocysteine methyltransferase in liver of hens (P>0.05). In conclusion, dietary methionine deficiency reduces performance of hens which may duo to the change of methionine metabolism pathway and the methylation of DNA and RNA.

Key words： methionine; laying hens; performance; methionine metabolism; methylation; gene expression

参考文献

[1] AKBARI MOGHADDAM KAKHKI R,GOLIAN A,ZARGHI H.Effect of digestible methionine+cystine concentration on performance,egg quality and blood metabolites in laying hens[J].British Poultry Science,2016,57(3):403-414.  

[2] BERTOLO R F,MCBREAIRTY L E.The nutritional burden of methylation reactions[J].Current Opinion in Clinical Nutrition and Metabolic Care,2013,16(1):102-108.  

[3] PERRONE C E,MALLOY V L,ORENTREICH D S,et al.Metabolic adaptations to methionine restriction that benefit health and lifespan in rodents[J].Experimental Gerontology,2013,48(7):654-660.  

[4] KIKUSATO M,SUDO S,TOYOMIZU M.Methionine deficiency leads to hepatic fat accretion via impairment of fatty acid import by carnitine palmitoyltransferase Ⅰ[J].British Poultry Science,2015,56(2):225-231.  

[5] JANKOWSKI J,KUBI? SKA M,ZDU? CZYK Z.Nutritional and immunomodulatory function of methionine in poultry diets-a review[J].Annals of Animal Science,2014,14(1):17-31.

[6] MATTOCKS D A L,MENTCH S J,SHNEYDER J,et al.Short term methionine restriction increases hepatic global DNA methylation in adult but not young male C57BL/6J mice[J].Experimental Gerontology,2017,88:1-8.

[7] 梁中军,王瑛,韩雪娇,等.饲粮蛋氨酸水平对蛋鸡产蛋后期生产性能的影响[J].动物营养学报,2015,27(9):2699-2704.

[8] SAKI A A,NASERI HARSINI R,TABATABAEI M M,et al.Estimates of methionine and sulfur amino acid requirements for laying hens using different models[J].Revista Brasileira de Ciência Avícola,2012,14(3):209-216.  

[9] HARMS R H,RUSSELL G B.Performance of commercial laying hens fed diets with various levels of methionine[J].The Journal of Applied Poultry Research,2003,12(4):449-455.  

[10] KESHAVARZ K.Effects of reducing dietary protein,methionine,choline,folic acid,and vitamin B12 during the late stages of the egg production cycle on performance and eggshell quality[J].Poultry Science,2003,82(9):1407-1414.  

[11] 吕明斌,孙作为,燕磊,等.肉仔鸡饲粮中蛋氨酸和半胱氨酸与赖氨酸适宜比例的研究[J].动物营养学报,2011,23(12):2109-2117.

[12] YODSERANEE R,BUNCHASAK C.Effects of dietary methionine source on productive performance,blood chemical,and hematological profiles in broiler chickens under tropical conditions[J].Tropical Animal Health and Production,2012,44(8):1957-1963.  

[13] ROBINSON J L,MCBREAIRTY L E,RANDELL E W,et al.Restriction of dietary methyl donors limits methionine availability and affects the partitioning of dietary methionine for creatine and phosphatidylcholine synthesis in the neonatal piglet[J].The Journal of Nutritional Biochemistry,2016,35:81-86.

[14] MATO J M,LU S C.Role of S-adenosyl-L-methionine in liver health and injury[J].Hepatology,2007,45(5):1306-1312.  

[15] WATERLAND R A.Assessing the effects of high methionine intake on DNA methylation[J].The Journal of Nutrition,2006,136(Suppl 6):1706S-1710S.

[16] JIA G F,FU Y,HE C.Reversible RNA adenosine methylation in biological regulation[J].Trends in Genetics,2013,29(2):108-115.  

[17] FINKELSTEIN J D.The metabolism of homocysteine:pathways and regulation[J].European Journal of Pediatrics,1998,157(Suppl.2):S40-S44.

[18] LIU G Q,ZONG K,ZHANG L L,et al.Dietary methionine affect meat qulity and myostatin gene exon 1 region methylation in skeletal muscle tissues of broilers[J].Agricultural Sciences in China,2010,9(9):1338-1346.  

[19] NIU Y M,ZHAO X,WU Y S,et al.N6-methyl-adenosine (m6A) in RNA:an old modification with a novel epigenetic function[J].Genomics,Proteomics & Bioinformatics,2013,11(1):8-17.  

[20] FU Y,DOMINISSINI D,RECHAVI G,et al.Gene expression regulation mediated through reversible m6A RNA methylation[J].Nature Reviews Genetics,2014,15(5):293-306.  

[21] LIU J Z,YUE Y N,HAN D L,et al.A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation[J].Nature Chemical Biology,2014,10(2):93-95.

[22] MARTINOV M V,VITVITSKY V M,MOSHAROV E V,et al.A substrate switch:a new mode of regulation in the methionine metabolic pathway[J].Journal of Theoretical Biology,2000,204(4):521-532.  

[23] BROSNAN J T,BROSNAN M E.The sulfur-containing amino acids:an overview[J].The Journal of Nutrition,2006,136(6):1636S-1640S.

[24] PILLAI P B,FANATICO A C,BEERS K W,et al.Homocysteine remethylation in young broilers fed varying levels of methionine,choline,and betaine[J].Poultry Science,2006,85(1):90-95.  

[25] MATO J M,LU S C.The hepatocarcinogenic effect of methionine and choline deficient diets:an adaptation to the Warburg effect?[J].Alcoholism:Clinical and Experimental Research,2011,35(5):811-814.  

[26] 高蔚娜,韩超,韦京豫,等.蛋氨酸负载对大鼠肝细胞Hcy代谢酶及相关代谢物的影响[J].营养学报,2013,35(6):545-548.

[27] TANG B Q,MUSTAFA A,GUPTA S,et al.Methionine-deficient diet induces post-transcriptional downregulation of cystathionine β-synthase[J].Nutrition,2010,26(11/12):1170-1175.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2