动物营养学报 >

2017 , Vol. 29 >Issue 9: 3287 - 3292

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.032

研究简报 Short communications

饲粮能量水平对后备母猪卵巢发育及相关基因表达的影响

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  • 黑龙江八一农垦大学动物科技学院, 大庆 163319
梁鸿雁(1969-),女,黑龙江鹤岗人,副教授,博士,从事动物营养与环境调控研究。E-mail:237298089@qq.com

收稿日期: 2017-02-22

  网络出版日期: 2017-09-08

基金资助

黑龙江八一农垦大学校级博士启动基金（校启B2009-20）

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Effects of Dietary Energy Level on Ovary Development and Related Gene Expression of Prepubertal Gilts

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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Da'qing 163319, China

Received date: 2017-02-22

  Online published: 2017-09-08

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摘要

本试验旨在研究饲粮能量水平对后备母猪卵巢发育及卵巢促卵泡素受体（FSHR）和促黄体素受体（LH/CGR）mRNA表达的影响。选择27头体重（61.0±3.1）kg的长×大二元母猪，随机分成3组（每组3个重复，每个重复3头猪），分别饲喂低、中、高3个能量水平[分别为NRC（1998）推荐消化能需要的90%、100%和110%]的饲粮。试验期内各组平均日采食量相同，但摄入的消化能水平不同。试验母猪在第2个发情期的第19天屠宰。结果表明：高能组卵巢重及大卵泡数显著高于低能组（P<0.05）；各组间小卵泡数量差异不显著（P>0.05）。高能组卵巢FSHR和LH/CGR mRNA表达量最高，显著高于低能组（P<0.05）。由此得出，高能量水平饲粮可促进后备母猪卵巢发育，有利于促进卵巢FSHR和LH/CGR mRNA的表达。

关键词： 能量水平; 后备母猪; 卵巢发育; 基因表达

本文引用格式

梁鸿雁, 赵春霞, 韩华 . 饲粮能量水平对后备母猪卵巢发育及相关基因表达的影响[J]. 动物营养学报, 2017 , 29(9) : 3287 -3292 . DOI: 10.3969/j.issn.1006-267x.2017.09.032

Abstract

This study aimed to study the effects of dietary energy level on ovary development and mRNA expression of follicle stimulating hormone receptor (FSHR) and luteinizing hormone/chorionic gonadotropin receptor (LH/CGR) of prepubertal gilts. Twenty-seven crossbred (Landrace×Yorkshire) gilts with the body weight of (61.0±3.1) kg were selected and randomly allocated to 3 groups with 3 replicates each and 3 pigs in each replicate, and were fed diets with different energy levels which were 90%, 100% and 110% digestible energy (DE) recommended by NRC (1998), respectively. Gilts in all groups were taken the same average daily feed intake in experimental period, but got different DE. Gilts were slaughtered on the 19th day of the 2nd estrus cycle. The results showed that ovary weight and the number of large follicles were significantly higher in the high energy group than those in the low energy group (P<0.05). Whereas the number of small follicles had no significant difference among the three energy level groups (P>0.05). The high energy group had the highest FSHR and LH/CGR mRNA expression levels, which were significantly higher than those in the low energy group (P<0.05). In conclusion, higher dietary energy level has significant positive effects on ovary development, and promotes the expression of FSHR and LH/CGR in ovary of prepubertal gilts.

Key words： energy level; prepubertal gilts; ovary development; gene expression

参考文献

[1] BOOTH P J,COSGROVE J R,FOXCROFT G R.Endodrine and metabolic responses to realimentation in feed-restricted prepubertal gilts:associations among gonadotropins,metabolic hormones,glucose,and uteroovarian development[J].Journal of Animal Science,1996,74(4):840-848.  

[2] BOOTH P J,CRAIGON J,FOXCROFT G R.Nutritional manipulation of growth and metabolic and reproductive status in prebupertal gilts[J].Journal of Animal Science,1994,72(9):2415-2424.  

[3] ARMSTRONG J D,BRITT J H.Nutritionally-induced anestrus in gilts:metabolic and endocrine changes associated with cessation and resumption of estrous cycles[J].Journal of Animal Science,1987,65(2):508-523.  

[4] ZAK L J,COSGROVE J R,AHERNE F X,et al.Pattern of feed intake and associated metabolic and endocrine changes differentially affect postweaning fertility in primiparous lactating sows[J].Journal of Animal Science,1997,75(1):208-216.  

[5] ZAK L J,XU X,HARDIN R T,et al.Impact of different patterns of feed intake during lactation in the primiparous sow on follicular development and oocyte maturation[J].Reproduction,1997,110(1):99-106.  

[6] VAN DEN BRAND H,DIELEMAN S J,SOEDE N M,et al.Dietary energy source at two feeding levels during lactation of primiparous sows:Ⅰ.Effects on glucose,insulin,and luteinizing hormone and on follicle development,weaning-to-estrus interval,and ovulation rate[J].Journal of Animal Science,2000,78(2):396-404.  

[7] ALMEIDA F R,KIRKWOOD R N,AHERNE F X,et al.Consequences of different patterns of feed intake during the estrous cycle in gilts on subsequent fertility[J].Journal of Animal Science,2000,78(6):1556-1563.  

[8] 王延忠.能量来源和水平对后备母猪卵母细胞质量及相关基因表达的影响[D].硕士学位论文.雅安:四川农业大学,2007:32-34.

[9] 周东胜.日粮能量水平和来源对后备母猪初情期启动、卵泡质量影响及机理研究[D].博士学位论文.雅安:四川农业大学,2013:39-44.

[10] HAZELEGER W,SOEDE N M,KERNP B.The effect of feeding strategy during the pre-folliculr phase on subsequent follicular development in the pig[J].Domestic Animal Endocrinology,2005,29(2):362-370.  

[11] 于淼瑛.不同日粮能量水平对初情期前母猪卵巢及子宫LH受体和FSH受体mRNA表达的影响[D].硕士学位论文.长春:吉林大学,2006:58.
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