研究简报 Short communications

低聚木糖对生长肥育猪血浆生化参数和肌肉脂肪酸组成的影响

展开
  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125;
    2. 湖南农业大学, 动物科学技术学院, 长沙 410128;
    3. 山东龙力生物科技股份有限公司, 禹城 251200
韩丽(1992-),女,河南周口人,硕士研究生,从事动物营养与饲料科学研究。E-mail:1404082917@qq.com

收稿日期: 2017-02-27

  网络出版日期: 2017-09-08

基金资助

国家973计划课题(2012CB124704);山东龙力生物科技股份有限公司合作项目;中央驻湘科研机构技术创新发展专项(2013TF3006)

Effects of Xylo-Oligosaccharide on Plasma Biochemical Parameters and Fatty Acid Composition of Muscle in Growing-Finishing Pigs

Expand
  • 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    3. Shandong Longli Biotechnology Co., Ltd., Yucheng 251200, China

Received date: 2017-02-27

  Online published: 2017-09-08

摘要

本试验旨在研究低聚木糖(XOS)对生长肥育猪血浆生化参数和肌肉脂肪酸组成的影响。选取70日龄、平均体重约为30 kg的杜×长×大三元杂交猪110头,随机分为11组,每组10头(公母各占1/2),单栏饲养。试验设对照组(饲喂基础饲粮),抗生素组(饲喂在基础饲粮中添加0.04 kg/t速大肥、0.2 kg/t抗敌素的饲粮),30~65 kg阶段100、250和500 g/t XOS添加组(在30~65 kg阶段分别饲喂在基础饲粮中添加100、250和500 g/t XOS的饲粮,在66~100 kg阶段均饲喂基础饲粮),66~100 kg阶段100、250和500 g/t XOS添加组(在30~65 kg阶段均饲喂基础饲粮,在66~100 kg阶段分别饲喂在基础饲粮中添加100、250和500 g/t XOS的饲粮)以及30~100 kg阶段100、250和500 g/t XOS添加组(分别饲喂在基础饲粮中添加100、250和500 g/t XOS的饲粮)。于试猪平均体重达100 kg(约170日龄)时,前腔静脉采血,离心分离血浆,测定生化参数;屠宰后取背最长肌和股二头肌样品,测定其脂肪酸组成。结果表明:与对照组相比,30~65 kg阶段,饲粮添加100或500 g/t XOS可显著降低股二头肌中十七烷酸(C17:0)含量(P<0.05);66~100 kg阶段,饲粮添加250 g/t XOS可显著增加股二头肌中饱和脂肪酸(SFA)+单不饱和脂肪酸(MUFA)含量(P<0.05),添加100或500 g/t XOS可显著增加股二头肌中花生烯酸(C20:1)含量(P<0.05);30~100 kg阶段,饲粮添加100 g/t XOS可显著增加背最长肌中油酸/亚油酸以及股二头肌中C20:1、MUFA和SFA+MUFA含量(P<0.05),添加100或250 g/t XOS可显著降低血浆总胆固醇浓度(P<0.05),添加500 g/t XOS可显著增加血浆高密度脂蛋白-胆固醇浓度(P<0.05)。综上所述,饲粮添加一定剂量的XOS可通过调控与脂代谢相关的血浆生化参数、增加肌肉中MUFA和SFA+MUFA含量而改善猪肉的风味和营养价值,且以30~100 kg阶段添加100 g/t XOS为最佳。

本文引用格式

韩丽, 潘杰, 张婷, 解培峰, 丁浩, 黄兴国, 孔祥峰 . 低聚木糖对生长肥育猪血浆生化参数和肌肉脂肪酸组成的影响[J]. 动物营养学报, 2017 , 29(9) : 3316 -3324 . DOI: 10.3969/j.issn.1006-267x.2017.09.036

Abstract

The present study was conducted to investigate the effects of xylo-oligosaccharide (XOS) on plasma biochemical parameters and fatty acid composition of muscle in growing-finishing pigs. A total of 110 Duroc×Large White×Landrace pigs at 70 days of age with an initial body weight about 30 kg were randomly assigned to 11 groups with 10 pigs per group (male:female=1:1), and fed individually. The experimental groups consisted of control group (pigs were fed a basal diet), antibiotic group (pigs were fed the basal diet supplemented with 0.04 kg/t Stafac and 0.2 kg/t Acrasin), supplementation groups of 100, 250 or 500 g/t XOS during 30 to 65 kg stage (pigs were fed the basal diet supplemented with 100, 250 or 500 g/t XOS during 30 to 65 kg stage, and were fed the basal diet during 66 to 100 kg stage), supplementation groups of 100, 250 or 500 g/t XOS during 66 to 100 kg stage (pigs were fed the basal diet during 30 to 65 kg stage, and were fed the basal diet supplemented with 100, 250 or 500 g/t XOS during 66 to 100 kg stage), and supplementation groups of 100, 250 or 500 g/t XOS during 30 to 100 kg stage (pigs were fed the basal diet supplemented with 100, 250 or 500 g/t XOS). When the average body weight of pigs reached about 100 kg (about 170 days of age), blood samples were collected by precaval vein, and then plasma were obtained by centrifuge for analyzing biochemical parameters. The samples of longissimus dorsi (LD) muscle and biceps femoris (BF) muscle were collected and their fatty acid composition were measured. The results showed that, when compared with the control group, supplementation with 100 or 500 g/t XOS significantly decreased the daturic acid (C17:0) content of BF muscle during 30 to 65 kg stage (P<0.05); during 66 to 100 kg stage, supplementation with 250 g/t XOS significantly increased the content of saturated fatty acid (SFA)+monoun saturated fatty acid (MUFA) of BF muscle (P<0.05), and supplementation with 100 or 500 g/t XOS significantly increased the content of arachidomic acid (C20:1) of BF muscle (P<0.05); during 30 to 100 kg stage, supplementation with 100 g/t XOS significantly increased the ratio of oleic acid (C18:1n9) to linoleic acid (C18:2n6c) of LD muscle (P<0.05), as well as the contents of C20:1, MUFA and SFA+MUFA of BF muscle, supplementation with 100 or 250 g/t XOS significantly decreased the plasma total cholesterol concentration (P<0.05), and supplementation with 500 g/t XOS significantly increased the plasma high-density lipoprotein-cholesterol concentration (P<0.05). Collectively, these findings suggest that diet supplemented with several doses of XOS can increase the muscular contents of MUFA and SFA+MUFA by regulating plasma biochemical parameters related to fat metabolism, which improve the flavor and nutritive value of pork; dietary supplementation with 100 g/t XOS is optimal during 30 to 100 kg stage in growing-finishing pigs.

参考文献

[1] WU X,RUAN Z,KONG X F,et al.Functions of microbiota in monogastric gastrointestinal tract and new practices in animal production[J].Journal of Food Agriculture & Environment, 2010,8(2):695-702.

[2] 马建爽,常文环,张姝,等.甜菜碱对肉鸡生长性能、脂质代谢及肌肉风味品质的影响[J].动物营养学报,2015,27(1):185-195.

[3] 谭利伟,管昶,周鲁宁,等.壳寡糖在动物营养中的研究与应用[J].中国畜牧杂志,2016,52(17):91-97.

[4] SMIRICKY-TJARDES M R,FLICKINGER E A,GRIESHOP C M,et al.In vitro fermentation characteristics of selected oligosaccharides by swine fecal microflora[J].Journal of Animal Science,2003,81(10):2505-2514.

[5] ZHOU X L,KONG X F,YANG X J,et al.Soybean oligosaccharides alter colon short-chain fatty acid production and microbial population in vitro[J].Journal of Animal Science 2012,90(Suppl.4):37-39.

[6] CARVALHO A F A,DE OLIVA NETO P,DE SILVA D F,et al.Xylo-oligosaccharides from lignocellulosic materials:chemical structure,health benefits and production by chemical and enzymatic hydrolysis[J].Food Research International,2013,51(1):75-85.

[7] 谭兵兵,姬玉娇,丁浩,等.低聚木糖对断奶仔猪生长性能、腹泻率和血浆生化参数的影响[J].动物营养学报,2016,28(8):2556-2563.

[8] 扶国才,贺生中,周岩民,等.低聚木糖对生长猪免疫功能和血液生化指标的影响[J].扬州大学学报:农业与生命科学版,2009,30(3):50-53.

[9] 潘杰,韩丽,孔祥峰,等.低聚木糖对生长肥育猪生长性能、胴体性状和肉品质的影响[J].动物营养学报,2017,29(7):2475-2481.

[10] 周笑犁,傅德智,孔祥峰,等.大豆寡糖对断奶环江香猪生长性能和营养物质代谢的影响[J].天然产物研究与开发,2012,24(1):98-101.

[11] 吴琛,刘俊锋,孔祥峰,等.日粮添加精氨酸生素对环江香猪肉质及抗氧化功能的影响[J].天然产物研究与开发,2011,23(5):901-904.

[12] STRANGE R J,SCHRECK C B,GOLDEN J T.Corticoid stress responses to handling and temperature in salmonids[J].Transactions of the American Fisheries Society,1977,106(3):213-218.  

[13] 祝倩,孔祥峰,姬玉娇,等.高、低营养水平饲粮对妊娠环江香猪繁殖性能、体成分和血浆生化参数的影响[J].动物营养学报,2016,28(5):1534-1540.

[14] 查伟,孔祥峰,谭敏捷,等.饲粮添加脯氨酸对妊娠环江香猪繁殖性能和血浆生化参数的影响[J].动物营养学报,2016,28(2):579-584.

[15] KESER O,BILAL T,KUTAY H C,et al.Effects of chitosan oligosaccharide and/or beta-glucan supplementation to diets containing organic zinc on performance and some blood indices in broilers[J].Pakistan Veterinary Journal,2012,32(1):15-19.

[16] 杨海玲,曾勇庆,魏述东,等.莱芜猪肌肉脂肪酸组成的发育性变化及其对肉质特性的影响[J].中国畜牧杂志,2006,42(5):18-21.

[17] WINZELL M S,SVENSSON H,ARNER P,et al.The expression of hormone-sensitive lipase in clonal β-cells and rat islets is induced by long-term exposure to high glucose[J].Diabetes,2001,50(10):2225-2230.  

[18] WU Y B,RAVINDRAN V,THOMAS D G,et al.Influence of method of whole wheat inclusion and xylanase supplementation on the performance,apparent metabolisable energy,digestive tract measurements and gut morphology of broilers[J].British Poultry Science,2004,45(3):385-394.  
文章导航

/