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枯草芽孢杆菌芽孢表面展示外源功能蛋白的应用

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  • 1. 四川农业大学动物医学院, 成都 611130;
    2. 成都农业科技职业学院畜牧兽医分院, 成都 611130
冯杰(1992-),男,四川自贡人,硕士研究生,从事动物微生态学研究。E-mail:fengjie9201@163.com

收稿日期: 2017-04-19

  网络出版日期: 2017-10-31

基金资助

“四川省高等学校科技创新团队”资助项目(KM406183.1)

Application of Bacillus subtilis Spore Surface Display of Functional Heterologous Protein

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  • 1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Branch College of Animal Husbandry and Veterinar, Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611130, China

Received date: 2017-04-19

  Online published: 2017-10-31

摘要

枯草芽孢杆菌是一种好氧的可直接用于人和动物的益生菌菌种,在不利条件下可以被诱导产生芽孢。芽孢具有特殊的构造及独特的生理特征,研究者们发现枯草芽孢杆菌芽孢是酶和免疫原等外源性功能蛋白的理想的锚定载体,以枯草芽孢杆菌芽孢衣壳蛋白作为分子载体,直接利用芽孢吸附作用和共价固定等方法使外源蛋白锚定在芽孢表面。目前已有多种酶蛋白、抗原蛋白和其他功能蛋白成功展示在枯草芽孢杆菌芽孢表面。本文主要对枯草芽孢杆菌芽孢结构及芽孢表面展示外源蛋白技术的策略和应用前景进行阐述。

本文引用格式

冯杰, 王振华, 潘康成 . 枯草芽孢杆菌芽孢表面展示外源功能蛋白的应用[J]. 动物营养学报, 2017 , 29(11) : 3893 -3898 . DOI: 10.3969/j.issn.1006-267x.2017.11.008

Abstract

Bacillus subtilis is an aerobic positive bacteria which is used as a probiotic for both human and animal. It can be induced to differentiate into the spore by unfavorable condition of circumstances. Because of the special structure and physiological characteristics of Bacillus subtilis spore, researchers found that the spore was the ideal vector of enzymes and antigens, and had been used the coat protein as a carrier, adsorption or covalent methods to display foreign proteins on the surface of the spore. Now, some enzymes, antigens and other functional proteins have successes to be displayed on the spore. The paper introduced the structure of Bacillus subtilis spore, the technology of spore surface display and its application.

参考文献

[1] ISTICATO R,CANGIANO G,TRAN H T,et al.Surface display of recombinant proteins on Bacillus subtilis spores[J].Journal of Bacteriology,2001,183(21):6294-6301.  

[2] ISTICATO R,RICCA E.Spore surface display[J].Microbiology Spectrum,2014,2(5):351-366.

[3] ZHOU Z,GONG S,LI X M,et al.Expression of Helicobacter pylori urease B on the surface of Bacillus subtilis spores[J].Journal of Medical Microbiology,2015,64(1):104-110.

[4] CHEN H,CHEN Z,NI Z,et al.Display of Thermotoga maritima MSB8 nitrilase on the spore surface of Bacillus subtilis using out coat protein CotG as the fusion partner[J].Journal of Molecular Catalysis B:Enzymatic,2016,123:73-80.

[5] HINC K,IWANICKI A,OBUCHOWSKI M.New stable anchor protein and peptide linker suitable for successful spore surface display in B.subtilis[J].Microbial Cell Factories,2013,12(1):22.

[6] HOSSEINI-ABARI A,KIM B G,LEE S H,et al.Surface display of bacterial tyrosinase on spores of Bacillus subtilis using CotE as an anchor protein[J].Journal of Basic Microbiology,2016,56(12):1331-1337.  

[7] POTOT S,SERRA C R,HENRIQUES A O,et al.Display of recombinant proteins on Bacillus subtilis spores,using a coat-associated enzyme as the carrier[J].Applied and Environmental Microbiology,2010,76(17):5926-5933.  

[8] HUANG J M,HONG H A,VAN TONG H,et al.Mucosal delivery of antigens using adsorption to bacterial spores[J].Vaccine,2010,28(4):1021-1030.  

[9] GHAEDMOHAMMADI S,RIGI G,ZADMARD R,et al.Immobilization of bioactive protein A from Staphylococcus aureus (SpA) on the surface of Bacillus subtilis spores[J].Molecular Biotechnology,2015,57(8):756-766.  

[10] TAN I S,RAMAMURTHI K S.Spore formation in Bacillus subtilis[J].Environmental Microbiology Reports,2014,6(3):212-225.  

[11] HIGGINS D,DWORKIN J.Recent progress in Bacillus subtilis sporulation[J].FEMS Microbiology Reviews,2012,36(1):131-148.  

[12] SETLOW P.I will survive:DNA protection in bacterial spores[J].Trends in Microbiology,2007,15(4):172-180.  

[13] SLIEMAN T A,NICHOLSON W L.Role of dipicolinic acid in survival of Bacillus subtilis spores exposed to artificial and solar UV radiation[J].Applied and Environmental Microbiology,2001,67(3):1274-1279.  

[14] MAGGE A,GRANGER A C,WAHOME P G,et al.Role of dipicolinic acid in the germination,stability,and viability of spores of Bacillus subtilis[J].Journal of Bacteriology,2008,190(14):4798-4807.  

[15] GILL R L,CASTAING J P,HSIN J,et al.Structural basis for the geometry-driven localization of a small protein[J].Proceedings of the National Academy of Sciences,2015,112(15):E1908-E1915.

[16] RAMAMURTHI K S,CLAPHAM K R,LOSICK R.Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis[J].Molecular Microbiology,2006,62(6):1547-1557.  

[17] RAMAMURTHI K S,LECUYER S,STONE H A,et al.Geometric cue for protein localization in a bacterium[J].Science,2009,323(5919):1354-1357.  

[18] MCKENNEY P T,DRIKS A,EICHENBERGER P.The Bacillus subtilis endospore:assembly and functions of the multilayered coat[J].Nature Reviews Microbiology,2013,11(1):33-44.

[19] ROSALES-MENDOZA S,ANGULO C.Bacillus subtilis comes of age as a vaccine production host and delivery vehicle[J].Expert Review of Vaccines,2015,14(8):1135-1148.

[20] SCHUMANN W.Production of recombinant proteins in Bacillus subtilis[J].Advances in Applied Microbiology,2007,62:137-189.

[21] YANG M M,ZHANG W W,ZHANG X F,et al.Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis[J].Biotechnology Letters,2006,28(21):1713-1718.  

[22] NGUYEN Q A,SCHUMANN W.Use of IPTG-inducible promoters for anchoring recombinant proteins on the Bacillus subtilis spore surface[J].Protein Expression and Purification,2014,95(3):67-76.

[23] RICCA E,BACCIGALUPI L,CANGIANO G,et al.Mucosal vaccine delivery by non-recombinant spores of Bacillus subtilis[J].Microbial Cell Factories,2014,13(1):115.

[24] DONADIO G,LANZILLI M,SIREC T,et al.Localization of a red fluorescence protein adsorbed on wild type and mutant spores of Bacillus subtilis[J].Microbial Cell Factories,2016,15(1):153.

[25] BATISTA M T,SOUZA R D,PACCEZ J D,et al.Gut adhesive Bacillus subtilis spores as a platform for mucosal delivery of antigens[J].Infection and Immunity,2014,82(4):1414-1423.  

[26] MOU C,ZHU L,XING X,et al.Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs[J].Antiviral Research,2016,131:74-84.

[27] DUC L H,HONG H A,Fairweather N,et al.Bacterial spores as vaccine vehicles[J].Infection and Immunity,2003,71(5):2810-2818.  

[28] 刘明刚,戴茜茜,徐毓琴,等.以CotB为分子载体表面展示鸡白痢沙门氏菌OmpC的重组枯草杆菌芽孢对小鼠免疫效果的研究[J].中国预防兽医学报,2015,37(12):966-969.

[29] SONG M,HONG H A,HUANG J M,et al.Killed Bacillus subtilis spores as a mucosal adjuvant for an H5N1 vaccine[J].Vaccine,2012,30(22):3266-3277.  

[30] APS L R M M,DINIZ M O,PORCHIA B F M M,et al.Bacillus subtilis spores as adjuvants for DNA vaccines[J].Vaccine,2015,33(20):2328-2334.  

[31] APS L R,TAVARES M B,ROZENFELD J H,et al.Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA[J].Journal of Biotechnology,2016,228:58-66.

[32] KIM J H,LEE C S,KIM B G.Spore-displayed streptavidin:a live diagnostic tool in biotechnology[J].Biochemical and Biophysical Research Communications,2005,331(1):210-214.  

[33] RICHTER A,KIM W,KIM J H,et al.Disulfide bonds of proteins displayed on spores of Bacillus subtilis can occur spontaneously[J].Current Microbiology,2015,71(1):156-161.  

[34] SIREC T,STRAZZULLI A,ISTICATO R,et al.Adsorption of β-galactosidase of Alicyclobacillus acidocaldarius on wild type and mutants spores of Bacillus subtilis[J].Microbial Cell Factories,2012,11(1):100.

[35] FALAHATI-POUR S K,LOTFI A S,AHMADIAN G,et al.Covalent immobilization of recombinant organophosphorus hydrolase on spores of Bacillus subtilis[J].Journal of Applied Microbiology,2015,118(4):976-988.  

[36] 冯凡.枯草芽孢杆菌表面展示人胰岛素原及其口服降血糖功效评估[D].博士学位论文.镇江:江苏大学,2015,64-78.
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