动物营养学报 >

2017 , Vol. 29 >Issue 11: 3899 - 3905

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.009

禽营养 Poultry nutrition

核黄素缺乏对1~28日龄北京鸭生长性能、器官指数和血浆生化指标的影响

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  • 中国农业科学院北京畜牧兽医研究所, 北京 100193
唐静(1986-),男,湖北荆州人,博士,研究方向为家禽营养。E-mail:tangjing198601@163.com

收稿日期: 2017-04-06

  网络出版日期: 2017-10-31

基金资助

现代水禽产业技术体系建设专项（CARS-42）

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Effects of Riboflavin Deficiency on Growth Performance, Organ Indexes and Plasma Biochemical Indices of Pekin Ducks from 1 to 28 Days of Age

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  • Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2017-04-06

  Online published: 2017-10-31

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摘要

本试验旨在研究核黄素缺乏对1~28日龄北京鸭生长性能、屠宰性能、器官指数、组织核黄素含量及血浆生化指标的影响。采用单因子完全随机试验设计，选取360只1日龄健康的雄性北京鸭，随机分为3个组，即核黄素缺乏组、采食配对组（人为控制该组采食量与核黄素缺乏组一致）和自由采食对照组，每组12个重复，每个重复10只鸭。核黄素缺乏组试验鸭饲喂核黄素缺乏饲粮（实测核黄素含量为1.38 mg/kg），采食配对组和自由采食对照组饲喂核黄素充足饲粮（在核黄素缺乏饲粮基础上添加10 mg/kg核黄素）。试验期为28 d。结果表明：与采食配对组和自由采食对照组相比，核黄素缺乏组试验鸭的平均日增重、胸肌率和腿肌率显著降低（P<0.05），料重比和死亡率显著升高（P<0.05），肝脏指数、心脏指数和胰腺指数显著提高（P<0.05），血浆核黄素、肝脏核黄素和黄素单核苷酸含量显著降低（P<0.05），血浆谷草转氨酶活性及总胆固醇和甘油三酯含量显著提高（P<0.05）。由此得出，核黄素是北京鸭生长发育必需的营养素；饲粮中缺乏核黄素可降低1~28日龄北京鸭的生长性能和组织中核黄素含量，可提高肝脏指数并导致血浆总胆固醇和甘油三酯含量及谷草转氨酶活性升高。

关键词： 北京鸭; 核黄素; 生长性能; 血浆生化指标; 器官指数

本文引用格式

唐静, 胡健, 江勇, 谢明, 侯水生 . 核黄素缺乏对1~28日龄北京鸭生长性能、器官指数和血浆生化指标的影响[J]. 动物营养学报, 2017 , 29(11) : 3899 -3905 . DOI: 10.3969/j.issn.1006-267x.2017.11.009

Abstract

This experiment was conducted to investigate the effects of riboflavin deficiency on growth performance, carcass traits, organ indexes, tissue riboflavin content and plasma biochemical indices of Pekin ducks from 1 to 28 days of age. Using a one-factor completely randomize design, a total of 360 one-day-old male Pekin ducks were divided into 3 groups with 12 replicates each group and 10 birds per replicate. The 3 groups were named as riboflavin-deficient group, pair-fed group and ad libitum control group, respectively, the ducks in riboflavin-deficient group were fed a riboflavin-deficient diet (the measured value of riboflavin content was 1.38 mg/kg), and the ducks in pair-fed group and ad libitum control group were fed a riboflavin-sufficient diet (added 10 mg/kg riboflavin to the riboflavin-deficient diet), and the feed intake of ducks in pair-fed group corresponded with the ducks in riboflavin-deficient group by artificial control. The experiment lasted for 28 days. The results showed as follows:compared with the pair-fed group and ad libitum control group, the average daily gain, breast muscle percentage and leg muscle percentage of ducks were significantly decreased in the riboflavin-deficient group (P<0.05), the feed/gain and mortality of ducks were significantly increased in the riboflavin-deficient group (P<0.05), the liver index, heart index and pancreas index of ducks were significantly increased in the riboflavin-deficient group (P<0.05), the contents of plasma riboflavin, liver riboflavin and flavin mononucleotide of ducks were significantly decreased in the riboflavin-deficient group (P<0.05), and the plasma total cholesterol and triglyceride content and aspartate transaminase activity of ducks were significantly increased in the riboflavin-deficient group (P<0.05). In conclusion, riboflavin is a essential nutrient for the growth and development of Pekin ducks. Dietary riboflavin deficiency can decrease the growth performance and tissue riboflavin content of Pekin ducks from 1 to 28 days of age, and increase the liver index and plasma total cholesterols and triglyceride contents and aspartate transaminase activity.

Key words： Pekin ducks; riboflavin; growth performance; plasma biochemical indices; organ indexes

参考文献

[1] POWERS H J.Riboflavin (vitamin B2) and health[J].The American Journal of Clinical Nutrition,2003,77(6):1352-1360.

[2] LIENHART W D,GUDIPATI V,MACHEROUX P.The human flavoproteome[J].Archives of Biochemistry and Biophysics,2013,535(2):150-162.  

[3] RUIZ N,HARMS R H.Riboflavin requirement of broiler chicks fed a corn-soybean diet[J].Poultry Science,1988,67(5):794-799.  

[4] CHUNG T K,BAKER D H.Riboflavin requirement of chicks fed purified amino acid and conventional corn-soybean meal diets[J].Poultry Science,1990,69(8):1357-1363.  

[5] DEYHIM F,BELAY T,TEETER R G.An evaluation of dietary riboflavin supplementation on growth rate,feed efficiency,ration metabolizable energy content,and glutathione reductase activity of broilers[J].Nutrition Research,1992,12(9):1123-1130.  

[6] 唐静,谢明,侯水生,等.1-21日龄不同性别北京鸭核黄素需要量的估测[J].动物营养学报,2012,24(4):661-668.

[7] 唐静,谢明,闻治国,等.核黄素对北京鸭生长性能和抗氧化机能的影响[J].动物营养学报,2013,25(12):2883-2887.

[8] 唐静,谢明,侯水生,等.日粮核黄素水平对1-21日龄北京鸭生长性能、抗氧化能力及激素分泌的影响[J].畜牧兽医学报,2012,43(11):1747-1753.

[9] BRITTON N L,RITER K E N L,SMALLIDGE R L,et al.Reversed-phase liquid chromatographic determination of riboflavin in feeds[J].Journal of AOAC International,2003,86(2):197-201.

[10] 韦京豫,郭长江,徐静,等.测定外周血核黄素及其衍生物含量的HPLC方法研究[J].营养学报,2006,28(1):79-82.

[11] KIM Y S,LAMBOOY J P.Biochemical and physiological changes in the rat during riboflavin deprivation and supplementation[J].The Journal of Nutrition,1969,98(4):467-476.

[12] OLPIN S,BATES C.Lipid metabolism in riboflavin-deficient rats:2.Mitochondrial fatty acid oxidation and the microsomal desaturation pathway[J].British Journal of Nutrition,1982,47(3):589-596.  

[13] PATTERSON B E,BATES C J.Riboflavin deficiency,metabolic rate and brown adipose tissue function in sucking and weanling rats[J].British Journal of Nutrition,1989,61(3):475-483.  

[14] DUERDEN J M,BATES C J.Effect of riboflavin deficiency on reproductive performance and on biochemical indices of riboflavin status in the rat[J].British Journal of Nutrition,1985,53(1):97-105.  

[15] BRADY P S,FENG Y X,BRADY L J.Transcriptional regulation of carnitine palmitoyltransferase synthesis in riboflavin deficiency in rats[J].The Journal of Nutrition,1988,118(9):1128-1136.

[16] BRADY P S,BRADY L J,PARSONS M J,et al.Effects of riboflavin deficiency on growth and glutathione peroxidase system enzymes in the baby pig[J].The Journal of Nutrition,1979,109(9):1615-1622.

[17] 张建海,原广华,庞全海,等.不同核黄素水平对肉仔鸡外周血细胞、免疫器官及生产性能的影响[J].山东家禽,2003(2):9-11.

[18] 蒋守群,周桂莲,林映才,等.饲粮维生素B2水平对1-21日龄黄羽肉鸡生长性能、免疫机能和抗氧化能力的影响[C]//第十四届全国家禽科学学术讨论会论文集.哈尔滨:中国农业技术出版社,2009:1015-1021.

[19] 王艳辉,王安,谢富.维生素B2对笼养蛋雏鸭生长性能、内分泌及抗氧化能力的影响[J].动物营养学报,2009,21(1):31-35.

[20] VEITCH K,DRAYE J P,VAMECQ J,et al.Altered acyl-CoA metabolism in riboflavin deficiency[J].Biochimica et Biophysica Acta:Lipids and Lipid Metabolism,1989,1006(3):335-343.  

[21] BURCH H B,HUNTER F E,Jr,COMBS A M,et al.Oxidative enzymes and phosphorylation in hepatic mitochondria from riboflavin-deficient rats[J].Journal of Biological Chemistry,1960,235:1540-1544.

[22] SURE B.Further observations on riboflavin as a food factor in economy of food utilization[J].The Journal of Nutrition,1941,22(3):295-301.

[23] SURE B,DICHEK M,CITRON M M.Riboflavin as a factor in economy of food utilization[J].The Journal of Nutrition,1941,21(5):453-460.

[24] 徐琪寿,韦京豫,杨继军,等.核黄素缺乏大鼠血浆与红细胞核黄素及衍生物浓度的变化[J].营养学报,2005,27(5):386-389.

[25] JACQUES P F,BOSTOM A G,WILSON P W,et al.Determinants of plasma total homocysteine concentration in the Framingham Offspring cohort[J].The American Journal of Clinical Nutrition,2001,73(3):613-621.

[26] HUSTAD S,MCKINLEY M C,MCKINULTY H,et al.Riboflavin,flavin mononucleotide,and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation[J].Clinical Chemistry,2002,48(9):1571-1577.

[27] MOAT S J,ASHFIELD-WATT P A,POWERS H J,et al.Effect of riboflavin status on the homocysteine-lowering effect of folate in relation to the MTHFR (C677T) genotype[J].Clinical Chemistry,2003,49(2):295-302.  

[28] ROSS N S,HANSEN T P.Riboflavin deficiency is associated with selective preservation of critical flavoenzyme-dependent metabolic pathways[J].Biofactors,1992,3(3):185-190.

[29] CHRISTENSEN H N.Riboflavin can protect tissues from oxidative injury[J].Nutrition Reviews,1993,51(5):149-150.

[30] LEVIN G,COGAN U,LEVY Y,et al.Riboflavin deficiency and the function and fluidity of rat erythrocyte membranes[J].The Journal of Nutrition,1990,120(8):857-861.

[31] 麻晓林,陈伟,金榕兵.肝损伤后检测肝功能指标的实验研究[J].创伤外科杂志,2003,5(1):20-22.

[32] TANIGUCHI M,YAMAMOTO T,NAKAMURA M.Effects of riboflavin deficiency on the lipids of rat liver mitochondria and microsomes[J].Journal of Nutritional Science and Vitaminology,1978,24:363-381.

[33] GIANAZZA E,VERGANI L,WAIT R,et al.Coordinated and reversible reduction of enzymes involved in terminal oxidative metabolism in skeletal muscle mitochondria from a riboflavin-responsive,multiple acyl-CoA dehydrogenase deficiency patient[J].Electrophoresis,2006,27(5/6):1182-1198.
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