动物营养学报 >

2017 , Vol. 29 >Issue 11: 3951 - 3961

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.015

反刍动物营养 Ruminant nutrition

精料中直链与支链淀粉比对羔羊瘤胃细菌菌群的影响

展开
  • 1. 黑龙江八一农垦大学动物科技学院, 大庆 163319;
    2. 扬州大学动物科学与技术学院, 扬州 225009
于洋洋(1992-),女,黑龙江双鸭山人,硕士研究生,从事饲料资源开发与利用研究。E-mail:739254249@qq.com

收稿日期: 2017-05-04

  网络出版日期: 2017-10-31

基金资助

国家自然科学基金面上项目（31372338）

收起

Effects of Amylose to Amylopectin Ratio in Concentrate on Rumen Bacterial Community in Lambs

Expand
  • 1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
    2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-05-04

  Online published: 2017-10-31

Fold

摘要

本文旨在研究精料中直链与支链淀粉比对不同日龄羔羊瘤胃细菌菌群的影响。选取48只体重无显著差异（P>0.05）的初生公羔，随机分成4组（每组3个重复，每个重复4只），各组精料中直链与支链淀粉比分别为0.12（木薯淀粉组）、0.23（玉米淀粉组）、0.24（小麦淀粉组）和0.48（豌豆淀粉组）。试验全期共77 d。于羔羊21、35、56和77日龄时，每个重复随机选择1只羔羊屠宰，采集瘤胃液样品。通过PCR-变性梯度凝胶电泳（DGGE）技术测定分析羔羊瘤胃细菌菌群的多样性。结果表明，不同直链与支链淀粉比精料组的羔羊于21、35、56和77日龄时，瘤胃细菌多样性指数、均匀度和丰富度均无显著性差异（P>0.05）。21日龄羔羊瘤胃细菌多样性指数显著高于35日龄（P<0.05），56和77日龄羔羊瘤胃细菌多样性指数有所升高，但在数值上仍低于21日龄羔羊。DGGE回收的条带多属厚壁菌门及拟杆菌门。56日龄的小麦淀粉组羔羊瘤胃内牛单胞月形菌含量较高。由此可见，不同直链与支链淀粉比精料未影响羔羊瘤胃优势细菌的结构，但饲喂小麦淀粉饲粮可促进56日龄羔羊瘤胃内牛单胞月形菌的生长。

关键词： 直链与支链淀粉比; 羔羊; PCR-变性梯度凝胶电泳; 瘤胃细菌菌群; 多样性指数

本文引用格式

于洋洋, 赵芳芳, 张爱忠, 姜宁, 李雁冰, 陈勇 . 精料中直链与支链淀粉比对羔羊瘤胃细菌菌群的影响[J]. 动物营养学报, 2017 , 29(11) : 3951 -3961 . DOI: 10.3969/j.issn.1006-267x.2017.11.015

Abstract

The objective of this study was to determine the effects of amylose to amylopectin ratio in concentrate on rumen bacterial community in lambs at different days of age. Forty-eight male lambs with no significant difference in body weight (P>0.05) were randomly divided into 4 groups, there were 3 replicates per group with 4 lambs per replicate. The concentrate amylose to amylopectin ratio of different groups was 0.12 (tapioca starch), 0.23 (corn starch), 0.24 (wheat starch) and 0.48 (pea starch), respectively. The whole experiment period was 77 days. One lamb from each replicate were randomly selected and slaughtered at 21, 35, 56 and 77 days of age to collect rumen fluid sample. The diversity of rumen bacterial community in lambs was determined by PCR-denaturing gradient gel electrophoresis (DGGE). The results showed that there was no effect on diversity index, evenness and richness of lambs at 21, 35, 56 and 77 days of age(P>0.05). Diversity index in lambs at 21 days of age was significant higher than that at 35 days of age (P<0.05),and it was increased at 56 and 77 days of age, but the numerical values were still lower than that at 21 days of age. Bands recovered from DGGE were almost belonged to Bacteroidetes and Firmicutes. Selenomonas bovis content in rumen in lambs at 56 days of age in wheat starch group was higher. Therefore, different amylose to amylopectin ratio in concentrate do not affect the structure of lamb rumen dominant bacteria, but feed wheat starch diet can promote the growth of Selenomonas bovis in rumen in lambs at 56 days of age.

Key words： amylose to amylopectin ratio; lamb; PCR-denaturing gradient gel electrophoresis; rumen bacterial community; diversity index

参考文献

[1] TESTER R F,KARKALAS J,QI X.Starch-composition,fine structure and architecture[J].Journal of Cereal Science,2004,39(2):151-165.  

[2] LIU C,WANG S J,COPELAND L,et al.Physicochemical properties and in vitro digestibility of starches from field peas grown in China[J].LWT:Food Science and Technology,2015,64(2):829-836.  

[3] 刘文.不同直/支链淀粉比对羔羊生长性能、养分消化率及肉质的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2015.

[4] REN W,ZHAO F F,ZHANG A Z,et al.Gastrointestinal tract development in fattening lambs fed diets with different amylose to amylopectin ratios[J].Canadian Journal of Animal Science,2016,96(3):425-433.  

[5] 赵芳芳,张爱忠,姜宁,等.淀粉对动物胃肠道菌群影响的研究进展[J].黑龙江畜牧兽医,2015(15):71-74.

[6] ZHAO W J,WANG Y P,LIU S Y,et al.The Dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments[J].PLoS One,2015,10(2):e0117441.

[7] NIU Q,LI P H,HAO S S,et al.Dynamic Distribution of the Gut microbiota and the relationship with apparent crude fiber digestibility and growth stages in pigs[J].Scientific Reports,2015,5:9938.

[8] ENGLYST H N,KINGMAN S M,CUMMINGS J H.Classification and measurement of nutritionally important starch fractions[J].European Journal of Clinical Nutrition,1992,46Suppl 2:S33-S50.

[9] HUO W J,ZHU W Y,MAO S Y.Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats[J].World Journal of Microbiology and Biotechnology,2014,30(2):669-680.  

[10] CALLAWAY T R,DOWD S E,EDRINGTON T S,et al.Evaluation of bacterial diversity in the rumen and feces of cattle fed different levels of dried distillers grains plus solubles using bacterial tag-encoded FLX amplicon pyrosequencing[J].Journal of Animal Science,2010,88(12):3977-3983.  

[11] ROSS E M,MOATE P J,BATH C R,et al.High throughput whole rumen metagenome profiling using untargeted massively parallel sequencing[J].BMC Genetics,2012,13:53.

[12] 岳喜新.蛋白水平及饲喂量对早期断奶羔羊生长性能及消化代谢的影响[D].硕士学位论文.阿拉尔:塔里木大学,2011.

[13] WALKER D M,WALKER G J.The development of the digestive system of the young animal.V.the development of rumen function in the young lamb[J].Journal of Agricultural Science,1959,53(3):374-380.  

[14] MINATO H,OTSUKA M,SHIRASAKA S,et al.Colonization of microorganisms in the rumen of young calves[J].Journal of General and Applied Microbiology,1992,38(5):447-456.  

[15] LIU K L,WANG J Q,BU D P,et al.Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen[J].Biochemical and Biophysical Research Communications,2009,385(4):605-611.  

[16] 张永婧,刘强,张文明,等.不同纤维来源饲粮和细胞壁降解酶对猪肠道微生物菌群多样性的影响[J].动物营养学报,2016,28(10):3275-3283.

[17] 田亚东,张大为,李敬,等.不同谷物饲粮对肉仔鸡肠道微生物群落多样性的影响[J].华北农学报,2013,28(4):184-189.

[18] SAKAMOTO M,TAKAGAKI A,MATSUMOTO K,et al.Butyricimonas synergistica gen.nov.,sp.nov.and Butyricimonas virosa sp.nov.,butyric acid-producing bacteria in the family ‘Porphyromonadaceae’ isolated from rat faeces[J].International Journal of Systematic and Evolutionary Microbiology,2009,59(7):1748-1753.  

[19] ZE X L.Degradation and utilization of resistant starch by microbiota in human large intestine[J].Journal of Dentistry,2013,41(1):60-70.  

[20] ZHANG K G,DONG X Z.Selenomonas bovis sp. nov.,isolated from yak rumen contents[J].International Journal of Systematic and Evolutionary Microbiology,2009,59(8):2080-2083.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2