动物营养学报 >

2017 , Vol. 29 >Issue 11: 3970 - 3979

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.017

水产营养 Aquaculture Nutrition

鸡肉粉替代鱼粉对黄颡鱼幼鱼生长性能、饲料利用、消化酶活性及抗氧化能力的影响

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  • 宁波大学海洋学院鱼类营养研究室, 宁波 315211
罗嘉翔(1995-),男,江西赣州人,硕士研究生,从事水生动物营养与饲料研究。E-mail:530197595@qq.com

收稿日期: 2017-04-25

  网络出版日期: 2017-10-31

基金资助

国家自然科学基金项目（41476125）

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Effects of Fish Meal Replacement with Poultry By-Product Meal on Growth Performance, Feed Utilization, Digestive Enzyme Activities and Antioxidant Capacity of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

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  • Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2017-04-25

  Online published: 2017-10-31

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摘要

本试验旨在研究鸡肉粉替代鱼粉对黄颡鱼（Pelteobagrus fulvidraco）幼鱼生长性能、饲料利用、消化酶活性及抗氧化能力的影响。配制6种等氮等脂的试验饲料（粗蛋白质含量为45%、粗脂肪含量为10%），6种试验饲料中鸡肉粉的添加量分别为0（对照组）、5.25%、10.51%、15.77%、21.03%和31.54%，替代鱼粉的比例分别为0、10%、20%、30%、40%和60%。选用初始体质量为（2.17±0.02） g的黄颡鱼幼鱼540尾，随机分为6组，每组3个重复，每个重复30尾，进行为期8周的养殖试验。结果显示：饲料中鸡肉粉替代鱼粉比例对黄颡鱼幼鱼成活率的影响不显著（P>0.05）。当鸡肉粉替代鱼粉比例由0增加到20%时，黄颡鱼幼鱼的增重率、特定生长率、饲料效率及蛋白质效率均显著升高（P<0.05）；而当鸡肉粉替代鱼粉比例由30%增加到60%时，黄颡鱼幼鱼的增重率、特定生长率、饲料效率及蛋白质效率则显著降低（P<0.05）。鸡肉粉替代鱼粉比例对黄颡鱼幼鱼的肥满度、脏体比和肠脂比无显著影响（P>0.05）。黄颡鱼幼鱼全鱼和肌肉干物质、粗蛋白质、粗脂肪、粗灰分含量在各组间均无显著差异（P>0.05）。对照组黄颡鱼幼鱼的胃蛋白酶活性显著低于其他各组（P<0.05）；鸡肉粉替代鱼粉比例大于10%时，黄颡鱼幼鱼的胃淀粉酶活性显著高于对照组（P<0.05）；10%鱼粉替代组黄颡鱼幼鱼的前肠淀粉酶活性显著高于对照组（P<0.05）；30%鱼粉替代组黄颡鱼幼鱼的肝脏淀粉酶活性显著高于对照组（P<0.05）。各鱼粉替代组黄颡鱼幼鱼血清谷草转氨酶活性与对照组无显著差异（P>0.05），而血清谷丙转氨酶活性及甘油三酯和总胆固醇含量在鸡肉粉替代30%鱼粉组达到最高值，且显著高于对照组（P<0.05）。黄颡鱼幼鱼肝脏超氧化物歧化酶活性在20%鱼粉替代组达到最高值，并显著高于对照组（P<0.05）；40%和60%鱼粉替代组肝脏丙二醛含量显著高于对照组（P<0.05）；鸡肉粉替代鱼粉对肝脏过氧化氢酶活性没有显著影响（P>0.05）。以增重率为评价指标，经折线模型分析得出，黄颡鱼幼鱼配合饲料中鸡肉粉替代鱼粉的最适比例为20.84%。

关键词： 黄颡鱼; 鸡肉粉; 鱼粉; 生长性能; 饲料利用; 抗氧化能力

本文引用格式

罗嘉翔, 黄文文, 袁野, 李晨晨, 朱婷婷, 周歧存 . 鸡肉粉替代鱼粉对黄颡鱼幼鱼生长性能、饲料利用、消化酶活性及抗氧化能力的影响[J]. 动物营养学报, 2017 , 29(11) : 3970 -3979 . DOI: 10.3969/j.issn.1006-267x.2017.11.017

Abstract

An 8-week feeding trial was conducted to evaluate the effects of fish meal (FM) replacement with poultry by-product meal (PBM) on growth performance, feed utilization, digestive enzyme activities and antioxidant capacity of juvenile yellow catfish (Pelteobagrus fulvidraco). Six iso-nitrogenous and iso-lipid diets (crude protein content was 45% and crude lipid content was 10%) were formulated with different levels of PBM, which were supplemented 0, 5.25%, 10.51%, 15.77%, 21.03% and 31.54% PBM to replace 0, 10%, 20%, 30%, 40% and 60% FM, respectively. A total of 540 juvenile yellow catfish with an initial body weight of (2.17±0.02) g were randomly divided into 6 groups with 3 replicates per group and 30 yellow catfish for each replicate. The results showed that survival ratio (SR) was not significantly influenced by the replacement ratio of FM by PBM (P>0.05). The weight gain ratio (WGR), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) of yellow catfish were significantly increased when the replacement ratio of FM by PBM increased from 0 to 20% (P<0.05), and they were significantly decreased while the replacement ratio of FM by PBM increased from 30% to 60% (P<0.05). The condition factor (CF), intraperitoneal fat ratio (IPE) and viscerosomtic index (VSI) of yellow catfish were not significantly influenced by the replacement ratio of FM by PBM (P>0.05). There were no significant differences in the contents of dry matter, crude protein, ash and crude lipid in muscle and whole body among groups (P>0.05). The stomach pepsin (PEP) activity of control group had significantly lower than the other groups (P<0.05), the stomach amylase (AMY) activity was significantly increased when the replacement ratio of FM by PBM more than 10% compared with control group (P<0.05), the foregut AMY activity of 10% FM replace group was significantly higher than of control group (P<0.05), and the liver AMY activity of 30% FM replace group was significantly higher than that of control group (P<0.05). The activity of aspartate aminotransferase (AST) in serum of all FM replace groups had no significant difference compared with control group (P>0.05), but the alanine aminotransferase (ALT) activity and triglyceride (TG) and total cholesterol (TC) contents in serum of 30% FM replace group had the highest values, and they were significantly higher than those of control group (P<0.05). The liver superoxide dismutase (SOD) activity reached the highest value in 20% FM replace group, and it was significantly higher than that of control group (P<0.05), and the liver malondialdehyde (MDA) content in 40% and 60% FM replace groups was significantly higher than that of control group (P<0.05). However, no significant difference was occurred at liver catalase (CAT) activity among all groups (P>0.05). Using WGR as the evaluation index, the optimal replacement ratio of FM with PBM is estimated to be 20.84% by broken-line model analysis.

Key words： Pelteobagrus fulvidraco; poultry by-product meal; fish meal; growth performance; feed utilization; antioxidant capacity

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