半胱胺对宁乡猪血清生化指标和肝脏脂肪代谢的影响

秦龙山, 邢月腾, 张杨, 陈宇光, 舒绪刚, 刘星亮, 张彬, 吴信

doi:10.3969/j.issn.1006-267x.2017.11.019

动物营养学报 >

2017 , Vol. 29 >Issue 11: 3987 - 3993

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.019

饲料营养 Feed science and technology

半胱胺对宁乡猪血清生化指标和肝脏脂肪代谢的影响

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1. 湖南农业大学动物科学技术学院, 湖南畜禽安全生产协同创新中心, 长沙 410128;
2. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术研究中心, 长沙 410125;
3. 仲恺农业工程学院动物科学技术学院, 广州 510225;
4. 宁乡县畜牧兽医水产局, 宁乡 410600

秦龙山(1992-),男,河南鹤壁人,硕士研究生,从事动物营养生理与代谢调控研究。E-mail:1400361065@qq.com

收稿日期: 2017-04-14

网络出版日期: 2017-10-31

基金资助

湖南省重点研发计划（2015NK2004，2015JC3100）；湖南省重大专项（2015NK1002）；宁乡县人民政府与湖南农业大学战略性合作项目

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Effects of Cysteamine on Serum Biochemical Indices and Liver Lipid Metabolism of Ningxiang Pigs

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1. Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Research Center of Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. College of Animal Science and Technology, Zhongkai University of Agricultural and Engineering, Guangzhou 510225, China;
4. Ningxiang Animal Husbandry, Veterinary and Aquatic Products Bureau, Ningxiang 410600, China

Received date: 2017-04-14

Online published: 2017-10-31

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摘要

本试验旨在研究饲粮中添加半胱胺对宁乡猪血清生化指标和肝脏脂肪代谢的影响。选取同批次平均体重约43.0 kg宁乡阉公猪30头，随机分为2组，每组5个重复，每个重复3头。对照组饲喂基础饲粮，试验组在基础饲粮中添加80 mg/kg半胱胺，试验期8周。结果表明：1）与对照组相比，试验组血清中葡萄糖含量提高了21.91%（P<0.01），低密度脂蛋白含量提高了26.22%（P<0.01），总胆固醇含量提高了9.75%（P<0.10），尿素氮含量降低了14.14%（P<0.05）。2）与对照组相比，试验组肝脏中饱和脂肪酸中肉豆蔻酸、棕榈酸和花生酸含量分别降低了72.09%（P<0.01）、13.12%（P<0.05）和71.13%（P<0.01），十七烷酸含量提高了11.77%（P<0.10）；单不饱和脂肪酸含量升高了40.37%（P<0.01），其中反油酸含量降低了85.90%（P<0.01），油酸含量提高了55.80%（P<0.01）；多不饱和脂肪酸含量降低了7.56%（P<0.05），其中亚油酸含量降低了18.56%（P<0.01），二十二碳六烯酸（DHA）和二十碳三烯酸含量分别提高了96.13%和37.99%（P<0.01）。3）与对照组相比，试验组过氧化物酶增殖体激活受体α（PPARα）表达量显著增加（P<0.05）。综上，在宁乡猪肥育猪饲粮中添加半胱胺能提高肝脏中DHA的含量，可能是通过调控肝脏脂肪代谢基因PPARα的表达，进而影响机体的脂肪代谢。

关键词： 半胱胺; 宁乡猪; 血清生化指标; 脂肪酸组成; 脂肪代谢

本文引用格式

秦龙山, 邢月腾, 张杨, 陈宇光, 舒绪刚, 刘星亮, 张彬, 吴信 . 半胱胺对宁乡猪血清生化指标和肝脏脂肪代谢的影响[J]. 动物营养学报, 2017 , 29(11) : 3987 -3993 . DOI: 10.3969/j.issn.1006-267x.2017.11.019

Abstract

This study was conducted to explore the effects of cysteamine (CS) on serum biochemical indices and lipid metabolism in liver of Ningxiang pigs. Thirty Ningxiang barrows with an average initial weight of 43 kg were randomly allocated into 2 groups (each group had 5 replicates with 3 pigs each). Pigs in the control group were fed a basal diet, and in the experimental group were fed the basal diet supplemented with 80 mg/kg CS. The trial lasted for 8 weeks. The results were showed as follows:1) compared with the control group, dietary supplementation with CS increased serum contents of glucose, low density lipoprotein and total cholesterol by 21.91% (P<0.01), 26.22% (P<0.01) and 9.75% (P<0.10), but significantly decreased serum urea nitrogen content by 14.14% (P<0.05). 2) Compared with the control group, dietary supplementation with CS significantly decreased the contents of saturated fatty acids such as C14:0, C16:0 and C20:0 by 72.09% (P<0.01), 13.12% (P<0.05) and 71.13% (P<0.01), but increased C17:0 content by 11.77% (P<0.10); and increased the monounsaturated fatty acid content by 40.37% (P<0.01) in which C18:1n9c content was increased by 55.80% (P<0.01), but C18:1n9t content was decreased by 85.90% (P<0.01); while decreased the polyunsaturated fatty acid content by 7.56% (P<0.05) in which C18:2n6c content was decreased by 18.56% (P<0.01), while contents of C22:6n3 (DHA) and C20:3n6 were increased by 96.13% and 37.99% (P<0.01) in liver. 3) Compared with the control group, dietary supplementation with CS significantly raised the gene expression level of peroxisome proliferator-activated receptor-α (PPARα) (P<0.05). It is concluded that diet supplementation with CS can increase the DHA content in liver, which may be related to the regulation of the expression of PPARα in liver tissue, and then affects the liver lipid metabolism.

Key words： cysteamine; Ningxiang pig; serum biochemical indices; fatty acid composition; lipid metabolism

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