饲粮中添加枯草芽孢杆菌或粪肠球菌对育成期水貂生长性能、营养物质消化率及氮代谢的影响

乜豪, 张铁涛, 崔虎, 王中成, 谢静静, 高秀华

doi:10.3969/j.issn.1006-267x.2017.11.021

动物营养学报 >

2017 , Vol. 29 >Issue 11: 4003 - 4009

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.021

饲料营养 Feed science and technology

饲粮中添加枯草芽孢杆菌或粪肠球菌对育成期水貂生长性能、营养物质消化率及氮代谢的影响

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1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点实验室, 北京 100081;
2. 中国农业科学院特产研究所, 长春 130112

乜豪(1992-),男,山东临沂人,硕士研究生,研究方向为单胃动物营养。E-mail:1300491157@qq.com

收稿日期: 2017-05-05

网络出版日期: 2017-10-31

基金资助

国家自然资源平台专项“特种动物种质资源平台”（201701）

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Effects of Dietary Bacillus subtilis or Enterococcus faecalis on Growth Performance, Nutrient Digestibility and Nitrogen Metabolism of Minks during Growing Period

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1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China;
2. Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Sciences, Changchun 130112, China

Received date: 2017-05-05

Online published: 2017-10-31

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摘要

本试验旨在研究饲粮中添加枯草芽孢杆菌或粪肠球菌对育成期水貂生长性能、营养物质消化率及氮代谢的影响。试验采用单因素完全随机试验设计，选取60日龄、健康、雄性水貂70只，平均体重为（957.37±93.96） g，随机分成7组，每组10个重复，每个重复1只。Ⅰ组为对照组，饲喂基础饲粮；Ⅱ、Ⅲ和Ⅳ组分别在基础饲粮中添加1×10⁹、1×10¹⁰和1×10¹¹ CFU/kg枯草芽孢杆菌，Ⅴ、Ⅵ和Ⅶ组分别在基础饲粮中添加1×108、1×10⁹和1×10¹⁰ CFU/kg粪肠球菌。预试期7 d，正试期60 d。结果表明：1）Ⅲ组的末重显著高于Ⅰ组（P<0.05），Ⅳ组的平均日采食量显著低于Ⅰ组（P<0.05），各组间平均日增重和料重比差异不显著（P>0.05）。2）Ⅱ、Ⅲ和Ⅴ组的干物质消化率显著高于Ⅰ组（P<0.05），Ⅴ组显著高于Ⅰ、Ⅳ、Ⅵ和Ⅶ组（P<0.05）；Ⅴ组的蛋白质消化率显著高于Ⅰ和Ⅶ组（P<0.05）；各组间脂肪消化率差异不显著（P>0.05）。3）Ⅳ、Ⅴ和Ⅵ组的粪氮含量显著低于Ⅰ组（P<0.05），Ⅴ和Ⅵ组的尿氮含量显著低于Ⅰ组（P<0.05）。各试验组的氮沉积均高于Ⅰ组，但是差异不显著（P>0.05）。Ⅲ和Ⅴ组的净蛋白质利用率显著高于Ⅰ组（P<0.05），Ⅲ组的蛋白质生物学价值显著高于Ⅰ组（P<0.05）。由此可见，育成期水貂饲粮中添加1×10¹⁰ CFU/kg枯草芽孢杆菌或1×10⁸ CFU/kg粪肠球菌时，水貂的生长性能、营养物质消化率、氮沉积、净蛋白质利用率和蛋白质生物学价值较为理想。

关键词： 枯草芽孢杆菌; 粪肠球菌; 水貂; 生长性能; 营养物质消化率; 氮代谢

本文引用格式

乜豪, 张铁涛, 崔虎, 王中成, 谢静静, 高秀华 . 饲粮中添加枯草芽孢杆菌或粪肠球菌对育成期水貂生长性能、营养物质消化率及氮代谢的影响[J]. 动物营养学报, 2017 , 29(11) : 4003 -4009 . DOI: 10.3969/j.issn.1006-267x.2017.11.021

Abstract

This experiment was conducted to study the effects of dietary Bacillus subtilis or Enterococcus faecalis on growth performance, nutrient digestibility and nitrogen metabolism of minks during growing period. The experiment was designed using a single factor complete randomized trial. Seventy healthy male 60-day-age minks with average weight of (957.37±93.96) g were randomly divided into 7 groups with 10 replicates per group and 1 mink per replicate. GroupⅠ (control group) was fed a basal diet, groups Ⅱ, Ⅲ and Ⅳ were fed the basal diet supplemented with 1×10⁹, 1×10¹⁰ and 1×10¹¹ CFU/kg Bacillus subtilis, and groups Ⅴ, Ⅵ and Ⅶ were fed the basal diet supplemented with 1×10⁸, 1×10⁹ and 1×10¹⁰ CFU/kg Enterococcus faecalis, respectively. The adaptation period lasted for 7 days and the formal trial period lasted for 60 days. The results showed as follows:1) the final weight of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05), the average daily feed intake of group Ⅵ was significantly lower than that of group Ⅰ(P<0.05), the average daily gain and feed to gain ratio had no significant difference among all groups (P>0.05). 2) The dry matter digestibility of groups Ⅱ, Ⅲ and Ⅴ was significantly higher than that of group Ⅰ (P<0.05), and the dry matter digestibility of group Ⅴ was significantly higher than that of groups Ⅰ, Ⅳ, Ⅵ and Ⅶ (P<0.05); the protein digestibility of group Ⅴ was significantly higher than that of groups Ⅰ and Ⅶ (P<0.05), the fat digestibility had no significant difference among all groups (P>0.05). 3) The fecal nitrogen content of groups Ⅳ, Ⅴ and Ⅵ was significantly lower than that of group Ⅰ(P<0.05), the urinary nitrogen content of groups Ⅴ and Ⅵ was significantly lower than that of group Ⅰ (P<0.05). The nitrogen deposition of test groups was higher than that of group Ⅰ, but had no significant difference (P>0.05). The net protein utilization of groups Ⅲ and Ⅴ was significantly higher than that of group Ⅰ (P<0.05), and the biological value of protein of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). In conclusion, when dietary supplemented with 1×10¹⁰ CFU/kg Bacillus subtilis or 1×10⁸ CFU/kg Enterococcus faecalis, the growth performance, nutrient digestibility, nitrogen deposition, net protein utilization and biological value of protein of minks are more preferably.

Key words： Bacillus subtilis; Enterococcus faecalis; minks; growth performance; nutrient digestibility; nitrogen metabolism

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