党参多糖对仔猪生长性能、血清细胞因子及肠黏膜分泌型免疫球蛋白A含量的影响

王希春, 朱电锋, 尹莉莉, 李玉, 冯士彬, 吴金节

doi:10.3969/j.issn.1006-267x.2017.11.028

动物营养学报 >

2017 , Vol. 29 >Issue 11: 4069 - 4075

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.028

研究简报 Short Communications

党参多糖对仔猪生长性能、血清细胞因子及肠黏膜分泌型免疫球蛋白A含量的影响

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安徽农业大学动物科技学院, 合肥 230036

王希春(1978-),男,安徽利辛人,副教授,博士,从事畜禽营养代谢病与中毒病研究。E-mail:wangxichun@ahau.edu.cn

收稿日期: 2017-05-03

网络出版日期: 2017-10-31

基金资助

安徽省生猪产业技术体系资助项目（2016-2020）

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Effects of Codonopsis pilosula Polysaccharide on Growth Performance, Serum Cytokines and Intestinal Mucosal Secretory Immunoglobulin A Contents of Piglets

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College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2017-05-03

Online published: 2017-10-31

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摘要

本试验旨在研究党参多糖（CPP）对仔猪生长性能、血清细胞因子及肠黏膜分泌型免疫球蛋白A （SIgA）含量的影响。选取1日龄"杜×长×大"三元杂交仔猪60头（6窝），随机分为3组，每组2个重复，每个重复10头猪（公母各占1/2）。14日龄时开始试验，3组仔猪分别饲喂仔猪代乳料（对照组）、仔猪代乳料＋1%党参多糖（低剂量组）、仔猪代乳料＋2%党参多糖（高剂量组），所有仔猪21日龄断奶，试验期14 d。结果表明：与对照组相比，1）21日龄时，饲粮添加2%党参多糖极显著提高了仔猪的平均体重（P<0.01）；28日龄时，饲粮添加1%和2%党参多糖显著提高了仔猪的平均体重（P<0.05）。22~28日龄时，饲粮添加2%党参多糖显著提高了仔猪的平均日增重（ADG）和平均日采食量（ADFI）（P<0.05）。2）21日龄时，饲粮添加2%党参多糖显著提高了仔猪的血清γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-6（IL-6）含量（P<0.05）；28日龄时，饲粮添加1%和2%党参多糖显著提高了仔猪的血清IL-2、白细胞介素-4（IL-4）和IL-6含量（P<0.05）。3）饲粮添加2%党参多糖极显著提高了仔猪的十二指肠、空肠和回肠黏膜SIgA含量（P<0.01）。由此可见，饲粮添加1%和2%党参多糖能够改善仔猪的生长性能，提高血清细胞因子和肠黏膜SIgA含量，其中添加2%党参多糖的作用效果优于1%党参多糖。

关键词： 党参多糖; 仔猪; 生长性能; 细胞因子; 肠黏膜

本文引用格式

王希春, 朱电锋, 尹莉莉, 李玉, 冯士彬, 吴金节 . 党参多糖对仔猪生长性能、血清细胞因子及肠黏膜分泌型免疫球蛋白A含量的影响[J]. 动物营养学报, 2017 , 29(11) : 4069 -4075 . DOI: 10.3969/j.issn.1006-267x.2017.11.028

Abstract

This experiment was conducted to investigate the effects of Codonopsis pilosula polysaccharide (CPP) on growth performance and the contents of serum cytokines and intestinal mucosal secretory immunoglobulin A (SIgA) of piglets. Sixty 1-day-old "Duroc×Landrace×Yorkshire" crossbred piglets from six litters were randomly divided into three groups with two replicates per group and ten piglets per replicate (males and females in half). Piglets in control group were fed with milk replacer, those in low dosage group were fed with milk replacer+1% CPP, and the others in high dosage group were fed with milk replacer+2% CPP from 14 days of age, respectively. All piglets were weaned at 21 days of age. The trial lasted for 14 days. The results showed as follows:compared with control group, 1) diet adding 2% CPP extremely significantly increased average body weight of piglets at 21 days of age (P<0.01), and diet adding 1% and 2% CPP significantly increased average body weight of piglets at 28 days of age (P<0.05). Diet adding 2% CPP significantly increased average daily gain (ADG) and average daily feed intake (ADFI) of piglets from 22 to 28 days of age (P<0.05). 2) Diet adding 2% CPP significantly increased the contents of interferon-γ (IFN-γ), interleukin-2 (IL-2) and interleukin-6 (IL-6) in serum of piglets at 21 days of age (P<0.05), and diet adding 1% and 2% CPP significantly increased the contents of IL-2, interleukin-4 (IL-4) and IL-6 in serum of piglets at 28 days of age (P<0.05). 3) Diet adding 2% CPP extremely significantly increased the content of duodenal, jejunum, and ileal mucosal SIgA (P<0.01). It is concluded that diet adding 1% and 2% CPP can improve the growth performance and the contents of serum cytokines and intestinal mucosal SIgA of piglets. The effect of adding 2% CPP is better than that of 1% CPP.

Key words： Codonopsis pilosula polysaccharide; piglets; growth performance; cytokine; intestinal mucosa

参考文献

[1] XU C, LIU Y, YUAN G X,et al.The contribution of side chains to antitumor activity of apolysaccharide from Codonopsis pilosula[J].International Journal of Biological Macromolecules,2012,50(4):891-894.

[2] 王敏,王彦春,洪小平,等.党参及其复方对亚急性衰老模型小鼠IL-2影响的研究[J].湖北中医杂志,2004,26(7):6-7.

[3] 陈嘉屿,胡林海,吴红梅,等.党参多糖类对荷瘤小鼠免疫应答及抑瘤作用研究[J].中华肿瘤防治杂志,2015,22(17):1357-1362.

[4] 彭梅,姚佳,杨晓玲,等.土党参多糖促进小鼠胃肠运动的初步研究[J].山地农业生物学报,2011,30(5):461-463.

[5] 曾丹,李嘉林,冯庆辉,等.党参提取物对母猪泌乳性能和仔猪生长性能的影响[J].猪业科学,2016,33(10):82-84.

[6] SALMINEN S, ISOLAURI E, SALMINEN E. Clinical uses of probiotics for stabilizing the gut mucosal barrier:successful strains and future challenges[J].Antonievan Leeuwenhoek,1996,70(2/3/4):347-358.

[7] 肖莉春,孔祥峰,郭小权.中药抑菌成分及其在养猪生产中的应用前景[J].动物营养学报,2012,24(12):2311-2318.

[8] SILVA R O, SANTANA A P M, CARVALHO N S, et al.A sulfated-polysaccharide fraction from seaweed Gracilaria birdiae prevents naproxen-induced gastrointestinal damage in rats[J].MarineDrugs,2012,10(12):2618-2633.

[9] 章红兵.党参对仔猪免疫功能的影响[J].中国猪业,2011,5(1):27-29.

[10] FANG L Q,GAO H M,ZHANG W N,et al.Resveratrol alleviates nerve injury after cerebral ischemia and reperfusion in mice by inhibiting inflammation and apoptosis[J].International Journal of Clinical and Experimental Medicine,2015,8(3):3219-3226.

[11] TSAI K H,LEE N H,CHEN G Y,et al.Dung-shen (Codonopsis pilosula) attenuated thecardiac-impaired insulin-like growth factor Ⅱ receptor pathway on myocardial cells[J].Food Chemistry,2013,138(2/3):1856-1867.

[12] XIANG L B,REHM K E,MARSHALL G D,Jr.Effects of strenuous exercise on Th1/Th2 gene expression from human peripheral blood mononuclear cells of marathon participant[J].Molecular Immunology,2014,60(2):129-134.

[13] MINGARI M C,GEROSA F,CARRA G,et al.Human interleukin-2 promotes proliferation of activated B cells via surface receptors similar to those of activated T cells[J].Nature,1984,312(5995):641-643.

[14] SAXENA R,KAUR J.Th1/Th2 cytokines and their genotypes as predictors of hepatitis B virus related hepatocellular carcinoma[J].World Journal of Hepatology,2015,7(11):1572-1580.

[15] LI X F,LIU X,TIAN L,et al.Cytokine-mediated immunopathogenesis of hepatitis B virus infections[J].Clinical Reviews in Allergy&Immunology,2016,50(1):41-54.

[16] LAN T,CHANG L,WU L,et al.IL-6 plays a crucial role in HBV infection[J].Journal of Clinical and Translational Hepatology,2015,3(4):271-276.

[17] CHENG L S,LIU Y,JIANG W. Restoring homeostasis of CD₄(+) T cells in hepatitis-B-virus-related liver fibrosis[J].World Journal of Gastroenterology,2015,21(38):10721-10731.

[18] 张晓君,祝晨,胡黎,等.党参多糖对小鼠免疫和造血功能的影响[J].中药新药与临床药理,2003,14(3):174-176.

[19] 曹丽,罗崇念,卞庆亚,等.党参多糖对鸡IL-2活性和淋巴细胞增殖反应的促进作用[J].中兽医医药杂志,2004,23(1):3-4.

[20] 林丹丹,秦韬,任喆,等.硒化党参多糖对免疫抑制小鼠免疫功能的影响[J].中国畜牧兽医,2016,43(6):1544-1549.

[21] 韩丽.新疆党参多糖对小鼠癌性恶病质的实验研究[J].北方药学,2010,7(3):17-18.

[22] 赵珊珊,熊善辉,黄鹏,等.板蓝根多糖对无初乳仔鼠十二指肠IgG⁺和SIgA⁺细胞表达的影响[J].中国实验动物学报,2012,20(1):47-50.

[23] 王雪,张圣方,赵龙玉,等.长根菇多糖对小鼠肠道菌群及分泌型IgA的影响[J].食品工业科技,2015,36(13):376-379,384.

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