研究简报 Short Communications

全自动体外模拟瘤胃发酵系统:19 kPa以下排气压力不影响发酵特征

展开
  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 湖南畜禽安全生产协同创新中心, 长沙 410128;
    3. 中国科学院亚热带农业生态研究所, 长沙 410125;
    4. 华南农业大学动物科学学院, 广州 510640
龙栋磊(1991-),男,湖南邵阳人,硕士研究生,从事反刍动物营养与繁殖研究。E-mail:15073147518@163.com

收稿日期: 2017-05-03

  网络出版日期: 2017-10-31

基金资助

国家自然科学基金项目(31561143009,31472133);国家科技计划项目(2016YFD0500504);湖南省科技计划项目(2015WK3043);中国科学院青年促进会项目

Automated Incubation System in Vitro:Venting Pressure Less Than 19 kPa did Not Affect Fermentation Characteristics

Expand
  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China;
    3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    4. College of Animal Science, South China Agricultural University, Guangzhou 510640, China

Received date: 2017-05-03

  Online published: 2017-10-31

摘要

本试验旨在研究不同排气压力对体外模拟瘤胃发酵特征的影响。选用3只体重相近、装有永久性瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体动物,利用全自动体外模拟瘤胃发酵系统,选用6种发酵底物进行48 h体外发酵,研究9、14和19 kPa 3种排气压力对总产气量、甲烷产量、产气参数、干物质消失率、发酵液pH以及挥发性脂肪酸产量等发酵参数的影响。结果表明:发酵底物对体外模拟瘤胃发酵参数影响显著(P<0.05);排气压力对体外模拟瘤胃发酵参数没有显著影响(P>0.05)。由此可见,排气压力由9 kPa增至19 kPa对体外模拟瘤胃发酵特征无影响。

本文引用格式

龙栋磊, 文江南, 王敏, 王荣, 张秀敏, 毛宏祥, 邓近平, 谭支良 . 全自动体外模拟瘤胃发酵系统:19 kPa以下排气压力不影响发酵特征[J]. 动物营养学报, 2017 , 29(11) : 4171 -4179 . DOI: 10.3969/j.issn.1006-267x.2017.11.041

Abstract

The present study was conducted to determine the effect of different venting pressure on in vitro fermentation characteristics. Rumen fluid was collected from three adult Xiaongdong black goats with similar weight and permanent rumen fistula, used automated incubation system in vitro, and six widely varied substrates were selected for 48 h in vitro fermentation, and three venting pressures (9, 14 and 19 kPa) were performed to investigate the effects of venting pressure on in vitro total gas production, methane production, in vitro dry matter disappearance, fermentation fluid pH and volatile fatty acid production et al. The results showed that fermentation substrate had significant effects on in vitro rumen fermentation parameters (P<0.05); however, venting pressure had no significant effects on in vitro fermentation parameters (P>0.05). In conclusion, venting pressure increase from 9 kPa to 19 kPa do not affect fermentation characteristics.

参考文献

[1] JOHNSON R R.Techniques and procedures for in vitro and in vivo rumen studies[J].Journal of Animal Science,1966,25(3):855-875.  

[2] FUCHIGAMI M,SENSHU T,HORIGUCHI M.A simple continuous culture system for rumen microbial digestion study and effects of defaunation and dilution rates[J].Journal of Dairy Science,1989,72(11):3070-3078.  

[3] RAAB L,CAFANTARIS B,JILG T,et al.Rumen protein degradation and biosynthesis 1. A new method for determination of protein degradation in rumen fluid in vitro[J].British Journal of Nutrition,1983,50(3):569-582.  

[4] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agricultural Science,1979,93(1):217-222.  

[5] PELL A N,SCHOFIELD P.Computerized monitoring of gas production to measure forage digestion in vitro[J].Journal of Dairy Science,1993,76(4):1063-1073.  

[6] MAURICIO R M,MOULD F L,DHANOA M S,et al.A semi-automated in vitro gas production technique for ruminant feedstuff evaluation[J].Animal Feed Science and Technology,1999,79(4):321-330.  

[7] CONE J W,VAN GELDER A H,BACHMANN H.Influence of inoculum source on gas production profiles[J].Animal Feed Science and Technology,2002,99(1/2/3/4):221-231.

[8] WANG M,WANG R,TANG S X,et al.Comparisons of manual and automated incubation systems:effects of venting procedures on in vitro ruminal fermentation[J].Livestock Science,2016,184:41-45.

[9] THEODOROU M K,WILLIAMS B A,DHANOA M S,et al.A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds[J].Animal Feed Science and Technology,1994,48(3/4):185-197.

[10] CATTANI M,TAGLIAPIETRA F,MACCARANA L,et al.Technical note:In vitro total gas and methane production measurements from closed or vented rumen batch culture systems[J].Journal of Dairy Science,2014,97(3):1736-1741.  

[11] WANG M,JANSSEN P H,SUN X Z,et al.A mathematical model to describe in vitro kinetics of H2 gas accumulation[J].Animal Feed Science and Technology,2013,184(1/2/3/4):1-16.

[12] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993:58-64.

[13] HALL M B,PELL A N,CHASE L E.Characteristics of neutral detergent-soluble fiber fermentation by mixed ruminal microbes[J].Animal Feed Science and Technology,1998,70(1/2):23-39.

[14] WANG M,SUN X Z,JANSSEN P H,et al.Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures[J].Animal Feed Science and Technology,2014,194:1-11.

[15] WANG M,TANG S X,TAN Z L.Modeling in vitro gas production kinetics:Derivation of Logistic-Exponential (LE) equations and comparison of models[J].Animal Feed Science and Technology,2011,165(3/4):137-150.

[16] WANG M,SUN X Z,TANG S X,et al.Deriving fractional rate of degradation of logistic-exponential (LE) model to evaluate early in vitro fermentation[J].Animal,2013,7(6):920-929.  

[17] 黄建军,顾平,陆彩霞.气相色谱法测定水相中微量氢气[J].分析试验室,2008,27(S2):369-372.

[18] HILL G A.Measurement of overall volumetric mass transfer coefficients for carbon dioxide in a well-mixed reactor using a pH probe[J].Industrial & Engineering Chemistry Research,2006,45(16):5796-5800.  

[19] LOERTING T,BERNARD J.Aqueous carbonic acid (H2CO3)[J].ChemPhysChem,2010,11(11):2305-2309.  

[20] DIJKSTRA J,ELLIS J L,KEBREAB E,et al.Ruminal pH regulation and nutritional consequences of low pH[J].Animal Feed Science and Technology,2012,172(1/2):22-33.

[21] WANG Y X,MAJAK W,MCALLISTER T A.Frothy bloat in ruminants:cause,occurrence,and mitigation strategies[J].Animal Feed Science and Technology,2012,172(1):103-114.

[22] COUNOTTE G H M,VAN'T KLOOSTER A T,VAN DER KUILEN J,et al.An analysis of the buffer system in the rumen of dairy cattle[J].Journal of Animal Science,1979,49(6):1536-1544.  

[23] TAGLIAPIETRA F,CATTANI M,BAILONI L,et al.In vitro rumen fermentation:effect of headspace pressure on the gas production kinetics of corn meal and meadow hay[J].Animal Feed Science and Technology,2010,158(3/):197-201.

[24] LOWMAN R S.Investigations into the factors which influence measurements during in vitro gas production studies[D].Ph.D.Thesis.Edinburgh:University of Edinburgh,1998.

[25] PATRA A K,YU Z.Effects of gas composition in headspace and bicarbonate concentrations in media on gas and methane production,degradability,and rumen fermentation using in vitro gas production techniques[J].Journal of Dairy Science,2013,96(7):4592-4600.  

[26] DIXON N M,KELL D B.The inhibition by CO2 of the growth and metabolism of micro-organisms[J].Journal of Applied Bacteriology,1989,67(2):109-136.  

[27] BAEZ A,FLORES N,BOLÍVAR F,et al.Simulation of dissolved CO2 gradients in a scale-down system:A metabolic and transcriptional study of recombinant Escherichia coli[J].Biotechnology Journal,2011,6(8):959-967.  

[28] BLOMBACH B,TAKORS R.CO2-intrinsic product,essential substrate,and regulatory trigger of microbial and mammalian production processes[J].Frontiers in Bioengineering and Biotechnology,2015,3:108.

[29] BLOMBACH B,BUCHHOLZ J,BUSCHE T,et al.Impact of different CO2/HCO- 3 levels on metabolism and regulation in Corynebacterium glutamicum[J].Journal of Biotechnology,2013,168(4):331-340.  

[30] CHENG K K,ZHAO X B,ZENG J,et al.Biotechnological production of succinic acid:current state and perspectives[J].Biofuels,Bioproducts and Biorefining,2012,6(3):302-318.  

[31] SONG H,LEE J W,CHOI S,et al.Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production[J].Biotechnology and Bioengineering,2007,98(6):1296-1304.  

[32] PRINS R A,VAN DER MEER P.On the contribution of the acrylate pathway to the formation of propionate from lactate in the rumen of cattle[J].Antonie van Leeuwenhoek,1976,42(1/2):25-31.
文章导航

/