猪营养 Swine Nutrition

饲粮维生素D添加形式对公猪繁殖性能的影响

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  • 1. 四川农业大学动物营养研究所, 成都 611130;
    2. 通威集团有限公司, 成都 610041
魏冬芹(1995-),女,重庆人,本科生,动物营养与饲养科学专业。E-mail:weidongqin2013@163.com

收稿日期: 2017-05-18

  网络出版日期: 2017-12-04

基金资助

畜禽繁殖调控新技术研发(2017YFD0501900);国家自然基金(31702128)

Effects of Dietary Vitamin D Forms on Reproductive Performance of Boars

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  • 1. Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;
    2. Tongwei Group Co., Ltd., Chengdu 610041, China

Received date: 2017-05-18

  Online published: 2017-12-04

摘要

本试验旨在研究饲粮维生素D添加形式对种用公猪繁殖性能的影响。选取16头18月龄的约克公猪,随机分成2组,2组饲粮中分别含有50 μg/kg维生素D3(VD3)和25-羟基维生素D3(25-OHD3),每组8个重复,每个重复1头猪。试验期16周。结果表明,与VD3组相比:1~16周,25-OHD3组公猪精子活力和每次射精的有效精子数显著提高(P<0.05),而精子畸形率显著降低(P<0.05);第112天,25-OHD3组血浆钙离子(Ca2+)和雌二醇含量及芳香化酶活性显著提高(P<0.05),精清25-OHD3、Ca2+、果糖含量和酸性磷酸酶的活性显著提高(P<0.05);25-OHD3组芳香化酶、维生素D 25-羟化酶、维生素D 24-羟化酶和维生素D受体基因的表达量显著升高(P<0.05)。综上所述,与同等水平VD3相比,种公猪饲粮中添加25-OHD3能更有效增加血浆维生素D含量,从而改善精子的形态和运动能力,提高公猪的繁殖性能。

本文引用格式

魏冬芹, 董洪俊, 吕刚, 吴德, 徐盛玉, 方正锋, 车炼强, 冯斌, 李健, 林燕 . 饲粮维生素D添加形式对公猪繁殖性能的影响[J]. 动物营养学报, 2017 , 29(12) : 4323 -4333 . DOI: 10.3969/j.issn.1006-267x.2017.12.012

Abstract

This study was conducted to investigate the effects of dietary vitamin D supplementation forms on reproductive performance of boars. Sixteen Yorkshire boars with eighteen months of age were randomly allocated to one of two groups, dietary contents of vitamin D3 (VD3) and 25-hydroxycholecalciferol (25-OHD3) were both 50 μg/kg in different groups, respectively. Each group had 8 replicates with 1 boar per replicate. The trial lasted for 16 weeks. The results showed that compared with VD3 group:sperm motility, effective sperm number per ejaculation in 25-OHD3 group were significantly increased in 1 to 16 weeks (P<0.05), while deformity ratio was significantly decreased (P<0.05); plasma calcium ion (Ca2+) content, estradiol content and aromatase activity in 25-OHD3 group were significantly increased at day 112 (P<0.05), and seminal plasma 25-OHD3 content, Ca2+ content, fructose content and acid phosphatase activity in 25-OHD3 group were significantly increased (P<0.05); the expression levels of aromatase, vitamin D 25-hydroxylase,vitamin D 24-hydroxylase and vitamin D receptor genes in 25-OHD3 group were significantly increased (P<0.05). In conclusion, compared with the same level of VD3, supplementation of 25-OHD3 in diet is conducive to increase plasma vitamin D content, so as to improve sperm morphology and athletic ability, and then improve reproductive performance of boars.

参考文献

[1] SMITAL J,WOLF J,DE SOUSA L L.Estimation of genetic parameters of semen characteristics and reproductive traits in AI boars[J].Animal Reproduction Science,2005,86(1/2):119-130.

[2] HUANG Y H,LO L L,LIU S H,et al.Age-related changes in semen quality characteristics and expectations of reproductive longevity in Duroc boars[J].Animal Science Journal,2010,81(4):432-437.  

[3] CLARK S G,SCHAEFFER D J,ALTHOUSE G C.B-mode ultrasonographic evaluation of paired testicular diameter of mature boars in relation to average total sperm numbers[J].Theriogenology,2003,60(6):1011-1023.  

[4] YESTE M,SANCHO S,BRIZ M,et al.A diet supplemented with L-carnitine improves the sperm quality of Piétrain but not of Duroc and Large White boars when photoperiod and temperature increase[J].Theriogenology,2010,73(5):577-586.  

[5] CASAS I,SANCHO S,BALLESTER J,et al.The HSP90AA1 sperm content and the prediction of the boar ejaculate freezability[J].Theriogenology,2010,74(6):940-950.  

[6] KUNAVONGKRIT A,SURIYASOMBOON A,LUNDEHEIM N,et al.Management and sperm production of boars under differing environmental conditions[J].Theriogenology,2005,63(2):657-667.  

[7] KWIECINSKI G G,PETRIE G I,DELUCA H F.Vitamin D is necessary for reproductive functions of the male rat[J].The Journal of Nutrition,1989,119(5):741-744.

[8] 杨宏伟.维生素D3在动物生产中研究进展[J].河南畜牧兽医,2016(4):16-18.

[9] COFFEY J D,HINES E A,STARKEY J D,et al.Feeding 25-hydroxycholecalciferol improves gilt reproductive performance and fetal vitamin D status[J].Journal of Animal Science,2012,90(11):3783-3788.  

[10] HINES E A,COFFEY J D,STARKEY C W,et al.Improvement of maternal vitamin D status with 25-hydroxycholecalciferol positively impacts porcine fetal skeletal muscle development and myoblast activity[J].Journal of Animal Science,2013,91(9):4116-4122.  

[11] ZHOU H,CHEN Y L,ZHOU Y,et al.Effects of 25-hydroxycholecalciferol supplementation in maternal diets on milk quality and serum bone status markers of sows and bone quality of piglets[J].Animal Science Journal,2017,88(3):476-483.  

[12] AUDET I,LAFOREST J P,MARTINEAU G P,et al.Effect of vitamin supplements on some aspects of performance,vitamin status,and semen quality in boars[J].Journal of Animal Science,2004,82(2):626-633.  

[13] WHO.WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction[M].Cambridge:Cambridge University Press,1999.

[14] HIRAI T,TSUJIMUAR A,UEDA T,et al.Effect of 1,25-dihydroxyvitamin D on testicular morphology and gene expression in experimental cryptorchid mouse:testis specific cDNA microarray analysis and potential implication in male infertility[J].The Journal of Urology,2009,181(3):1487-1492.  

[15] WING T Y,CHRISTENSEN A K.Morphometric studies on rat seminiferous tubules[J].American Journal of Anatomy,1982,165(1):13-25.  

[16] RUSSELL L D,PETERSON R N.Determination of the elongate spermatid-Sertoli cell ratio in various mammals[J].Journal of Reproduction and Fertility,1984,70(2):635-641.  

[17] HAMMOUD A O,MEIKLE A W,PETERSON C M,et al.Association of 25-hydroxy-vitamin D levels with semen and hormonal parameters[J].Asian Journal of Andrology,2012,14(6):855-859.  

[18] JENSEN M B,BJERRUM P J,JESSEN T E,et al.Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa[J].Human Reproduction,2011,26(6):1307-1317.  

[19] BAR A,RAZAPHKOVSKY V,VAX E,et al.Performance and bone development in broiler chickens given 25-hydroxycholecalciferol[J].British Poultry Science,2003,44(2):224-233.  

[20] WEBER G M,WITSCHI A K M,WENK C,et al.Effects of dietary 25-hydroxycholecalciferol and cholecalciferol on blood and vitamin D and mineral status,bone turnover,milk composition and reproductive performance of sows[J].Journal of Animal Science,2014,92(11):899-909.

[21] VERSTUYF A,CARMELIET G,BOUILLON R,et al.Vitamin D:a pleiotropic hormone[J].Kidney International,2010,78(2):140-145.  

[22] ERBEN R G,SOEGIARTO D W,WEBER K,et al.Deletion of deoxyribonucleic acid binding domain of the vitamin D receptor abrogates genomic and nongenomic functions of vitamin D[J].Molecular Endocrinology,2002,16(7):1524-1537.  

[23] BOUILLON R,CARMELIET G,VERLINDEN L,et al.Vitamin D and human health:lessons from vitamin D receptor null mice[J].Endocrine Reviews,2008,29(6):726-776.  

[24] JOHNSON J A,GRANDE J P,ROCHE P C,et al.Immunohistochemical detection and distribution of the 1,25-dihydroxyvitamin D3 receptor in rat reproductive tissues[J].Histochemistry and Cell Biology,1996,105(1):7-15.  

[25] CORBETT S T,HILL O,NANGIA A K.Vitamin D receptor found in human sperm[J].Urology,2006,68(6):1345-1349.  

[26] MAHMOUDI A R,ZARNANI A H,JEDDI-TEHRANI M,et al.Distribution of vitamin D receptor and 1α-hydroxylase in male mouse reproductive tract[J].Reproductive Sciences,2013,20(4):426-436.  

[27] AQUILA S,GUIDO C,MIDDEA E,et al.Human male gamete endocrinology:1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates different aspects of human sperm biology and metabolism[J].Reproductive Biology and Endocrinology,2009,7:140.
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