动物营养学报 >

2017 , Vol. 29 >Issue 12: 4372 - 4385

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.018

水产营养 Aquaculture Nutrition

80%赖氨酸硫酸盐与98%赖氨酸盐酸盐对生长中期草鱼生长性能、消化吸收能力和消化器官生长发育影响的比较研究

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  • 1. 四川农业大学动物营养研究所, 成都 611130;
    2. 成都农业科技职业学院畜牧兽医分院, 成都 611130;
    3. 鱼类营养与安全生产四川省高校重点实验室, 成都 611130;
    4. 动物抗病营养教育部重点实验室, 成都 611130
胡凯(1981-),男,四川雅安人,博士,从事动物营养与饲料科学研究。E-mail:linaturehu@126.com

收稿日期: 2017-05-05

  网络出版日期: 2017-12-04

基金资助

四川省重大科技成果转化示范（2015CC0011）；四川省青年创新团队（2017TD0002）

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A Comparative Study:Effects of 80% L-lysine·H2SO4 and 98% L-lysine·HCl on Growth Performance, Digestion and Absorption Capacities and Growth Development of Digestive Organs of Young Grass Carp (Ctenopharyngodon idella)

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  • 1. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;
    2. Department of Animal and Veterinary Science, Chengdu Agricultural College, Chengdu 611130, China;
    3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
    4. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-05-05

  Online published: 2017-12-04

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摘要

本试验通过比较80%赖氨酸硫酸盐[80%L-Lys·H2SO4，简称80赖氨酸（80-Lys）]与98%赖氨酸盐酸盐[98%L-Lys·HCl，简称98赖氨酸（98-Lys）]对生长中期草鱼生长性能、消化吸收能力和消化器官生长发育的影响，探讨80-Lys和98-Lys在草鱼上的生物效价并确定以80-Lys为赖氨酸（Lys）添加形式时饲料中Lys的适宜含量。选择初始体重为275.80 g左右的健康草鱼540尾，随机分成6组（每组3个重复，每个重复30尾鱼），分别饲喂Lys含量为0.8%（基础饲料）、1.0%、1.2%、1.4%和1.6%的添加80-Lys的饲料及Lys含量为1.2%的添加98-Lys的饲料60 d。结果表明：与基础饲料相比，饲料中添加适宜水平的80-Lys使饲料Lys含量达到1.2%时可显著提高生长中期草鱼的增重率（WGR），特定生长率（SGR），采食量（FI），全肠脂肪酶、淀粉酶活力，肝胰脏谷草转氨酶（GOT）和谷丙转氨酶（GPT）活力，前、中、后肠碱性磷酸酶（AKP）、肌酸激酶（CK）活力，肝体指数与肠体指数以及前、后肠皱襞高度（P<0.05），显著降低血清GOT和GPT活力（P<0.05），且80-Lys对上述指标的作用效果显著优于98-Lys（P<0.05）；此外，还可显著提高生长中期草鱼的饲料效率（FE），全肠胰蛋白酶活力，前、中、后肠Na+，K+-ATP酶（Na+，K+-ATPase）和γ-谷胺酰转肽酶（γ-GT）活力，肠长与肠长指数以及中肠皱襞高度（P<0.05），但80-Lys对上述指标的作用效果与98-Lys差异不显著（P>0.05）。由此得出，与98-Lys相比，80-Lys能更有效地提高生长中期草鱼的消化吸收能力，进而促进其生长。以80-Lys为Lys添加形式，以SGR和FE为标识，生长中期草鱼（276~667 g）饲料中Lys的最适含量分别为1.31%（占饲料蛋白质的4.68%）和1.27%（占饲料蛋白质的4.54%）。

关键词： 80%赖氨酸硫酸盐; 98%赖氨酸盐酸盐; 草鱼; 生长性能; 消化吸收能力

本文引用格式

胡凯, 苏玥宁, 冯琳, 刘扬, 姜维丹, 吴培, 姜俊, 周小秋 . 80%赖氨酸硫酸盐与98%赖氨酸盐酸盐对生长中期草鱼生长性能、消化吸收能力和消化器官生长发育影响的比较研究[J]. 动物营养学报, 2017 , 29(12) : 4372 -4385 . DOI: 10.3969/j.issn.1006-267x.2017.12.018

Abstract

This study aimed to compare the effects of 80% L-lysine·H2SO4 (80-lysine) and 98% L-lysine·HCl (98-lysine) on growth performance, digestion and absorption capacities and growth development of digestive organs of young grass carp (Ctenopharyngodon idella), to explore the biological values of 98-lysine and 98-lysine, and to determine the optimum dietary lysine content using 80-lysine as the supplemental form of lysine. A total of 540 grass carp with an initial body weight about 275.80 g were randomly divided into 6 groups with 3 replicates each and 30 fish per replicate. Fish in 6 groups were fed adding 80-lysine diets containing 0.8% (basal diet), 1.0%, 1.2%, 1.4% and 1.6% lysine and adding 98-lysine diet containing 1.2% lysine for 60 d, respectively. The results showed as follows:compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-lysine could significantly increase the weight gain rate (WGR), specific growth rate (SGR), feed intake (FI), the activities of lipase, amylase in total intestine, the activities of glutamic oxalacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) in hepatopancreas, the activities of alkaline phosphatase (AKP) and creatine kinase (CK)in proximal intestine (PI), mid intestine (MI) and distal intestine (DI), hepatosomatic index (HSI), intestosomatic index (ISI) and the fold height of PI and DI (P<0.05), and significantly decrease the activities of GOT and GPT in serum (P<0.05); the effects of 80-lysine on these parameters were better than 98-lysine (P<0.05). Moreover, compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-lysine could significantly increase the feed efficiency (FE), total intestine trypsin activity, the activities of Na+, K+-ATPase and γ-glutamyltransferase (γ-GT) in PI, MI and DI, intestinal length, relative gut length (RGL) and fold height in MI (P<0.05); however, compared with 98-lysine, no significant effects of 80-lysine were observed on these parameters (P>0.05). In conclusion, 80-lysine is more effective than 98-lysine for improving growth, and digestion and absorption capacities of young grass carp. Using 80-lysine as the supplemental form of lysine, the optimum dietary lysine content for young grass carp (276 to 667 g) base on SGR and FE is 1.31% (4.68% of dietary protein) and 1.27% (4.54% of dietary protein), respectively.

Key words： 80% L-lysine·H2SO4; 98% L-lysine·HCl; grass carp (Ctenopharyngodon idella); growth performance; digestion and absorption capacities

参考文献

[1] LI X Y,TANG L,HU K,et al.Effect of dietary lysine on growth,intestinal enzymes activities and antioxidant status of sub-adult grass carp (Ctenopharyngodon idella)[J].Fish Physiology and Biochemistry,2014,40(3):659-671.  

[2] ZHOU X Q,ZHAO C R,JIANG J,et al.Dietary lysine requirement of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture Nutrition,2008,14(5):381-386.  

[3] DENG D F,DOMINY W,JU Z Y,et al.Dietary lysine requirement of juvenile Pacific threadfin (Polydactylus sexfilis)[J].Aquaculture,2010,308(1/2):44-48.

[4] ZHANG C X,AI Q H,MAI K S,et al.Dietary lysine requirement of large yellow croaker,Pseudosciaena crocea R[J].Aquaculture,2008,283(1/2/3/4):123-127.

[5] MICHELATO M,DE OLIVEIRA VIDAL L,XAVIER T O,et al.Dietary lysine requirement to enhance muscle development and fillet yield of finishing Nile tilapia[J].Aquaculture,2016,457:124-130.

[6] ZHOU F,SHAO Q J,XIAO J X,et al.Effects of dietary arginine and lysine levels on growth performance,nutrient utilization and tissue biochemical profile of black sea bream,Acanthopagrus schlegelii,fingerlings[J].Aquaculture,2011,319(1/2):72-80.

[7] ZHOU X Q,ZHAO C R,LIN Y.Compare the effect of diet supplementation with uncoated or coated lysine on juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture Nutrition,2007,13(6):457-461.  

[8] 朱进龙,臧建军,曾祥芳,等.80赖氨酸与70赖氨酸和98赖氨酸对生长肥育猪饲喂效果的比较研究[J].中国畜牧杂志,2014,50(21):27-31.

[9] NIU J,CHEN X,LIN H Z,et al.Comparison of L-lysine·HCl and L-lysine sulphate in the feed of Penaeus monodon and re-evaluation of dietary lysine requirement for P. monodon[J].Aquaculture Research,2017,48(1):134-148.  

[10] 赵金鑫,李小勤,彭松,等.斑点叉尾鲖对不同形式赖氨酸利用的比较研究[J].水生生物学报,2016,40(1):19-26.

[11] RODEHUTSCORD M,BORCHERT F,GREGUS Z,et al.Availability and utilisation of free lysine in rainbow trout (Oncorhynchus mykiss):2.Comparison of L-lysine·HCl and L-lysine sulphate[J].Aquaculture,2000,187(1/2):177-183.

[12] SMIRICKY-TJARDES M R,MAVROMICHALIS I,ALBIN D M,et al.Bioefficacy of L-lysine sulfate compared with feed-grade L-lysine·HCl in young pigs[J].Journal of Animal Science,2004,82(9):2610-2614.  

[13] AHMAD G,MUSHTAQ T,MIRZA M A,et al.Comparative bioefficacy of lysine from L-lysine hydrochloride or L-lysine sulfate in basal diets containing graded levels of canola meal for female broiler chickens[J].Poultry Science,2007,86(3):525-530.  

[14] ANDERSON L C,LEWIS A J,PEO E R,Jr.,et al.Effect of various dietary arginine:lysine ratios on performance,carcass composition and plasma amino acid concentrations of growing-finishing swine[J].Journal of Animal Science,1984,58(2):362-368.  

[15] ALAM M S,TESHIMA S I,ISHIKAWA M,et al.Effects of dietary arginine and lysine levels on growth performance and biochemical parameters of juvenile Japanese flounder Paralichthys olivaceus[J].Fisheries Science,2002,68(3):509-516.  

[16] 农业部渔业渔政管理局.中国渔业统计年鉴2016[M].北京:中国农业出版社,2016.

[17] NRC.Nutrient requirements of fish and shrimp[S].Washington,D.C.:National Academies Press,2011.

[18] FENG L,LUO J B,JIANG W D,et al.Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency:regulation of tight junction protein transcript,antioxidant status and apoptosis-related gene expression[J].Fish & Shellfish Immunology,2015,45(2):239-249.  

[19] XU H J,JIANG W D,FENG L,et al.Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2,apoptosis,MLCK,NF-κB and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare[J].Fish & Shellfish Immunology,2016,58:177-192.

[20] XU H J,JIANG W D,FENG L,et al.Dietary vitamin C deficiency depresses the growth,head kidney and spleen immunity and structural integrity by regulating NF-κB,TOR,Nrf2,apoptosis and MLCK signaling in young grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2016,52:111-138.

[21] GERAYLOU Z,SOUFFREAU C,RURANGWA E,et al.Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance,non-specific immunity and gut microbiota of juvenile Siberian sturgeon (Acipenser baerii)[J].Fish & Shellfish Immunology,2013,35(3):766-775.  

[22] HUANG S S Y,STRATHE A B,WANG W F,et al.Selenocompounds in juvenile white sturgeon:evaluating blood,tissue,and urine selenium concentrations after a single oral dose[J].Aquatic Toxicology,2012,109:158-165.

[23] LIN Y,ZHOU, X Q.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture,2006,256(1/2/3/4):389-394.

[24] LI S Q,FENG L,JIANG W D,et al.Deficiency of dietary niacin decreases digestion and absorption capacities via declining the digestive and brush border enzyme activities and downregulating those enzyme gene transcription related to TOR pathway of the hepatopancreas and intestine in young grass carp (Ctenopharyngodon idella)[J].Aquaculture Nutrition,2016,22(6):1267-1282.  

[25] 唐凌,孙崇岩,邝声耀,等.晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力影响的比较[J].动物营养学报,2014,26(2):411-419.

[26] NGUYEN L,DAVIS D A.Comparison of crystalline lysine and intact lysine used as a supplement in practical diets of channel catfish (Ictalurus punctatus) and Nile tilapia (Oreochromis niloticus)[J].Aquaculture,2016,464:331-339.

[27] WANG S,LIU Y J,TIAN L X,et al.Quantitative dietary lysine requirement of juvenile grass carp Ctenopharyngodon idella[J].Aquaculture,2005,249(1/2/3/4):419-429.

[28] GAN L,LIU Y J,TIAN L X,et al.Effects of dissolved oxygen and dietary lysine levels on growth performance,feed conversion ratio and body composition of grass carp,Ctenopharyngodon idella[J].Aquaculture Nutrition,2013,19(6):860-869.  

[29] LUO Z,LIU Y J,MAI K S,et al.Quantitative L-lysine requirement of juvenile grouper Epinephelus coioides[J].Aquaculture Nutrition,2006,12(3):165-172.  

[30] PERES H,OLIVA-TELES A.Lysine requirement and efficiency of lysine utilization in turbot (Scophthalmus maximus) juveniles[J].Aquaculture,2008,275(1/2/3/4):283-290.

[31] 刘永坚,田丽霞,刘栋辉,等.实用饲料补充结晶或包膜赖氨酸对草鱼生长、血清游离氨基酸和肌肉蛋白质合成率的影响[J].水产学报,2002,26(3):252-258.

[32] TOME D.Protein,amino acids and the control of food intake[J].British Journal of Nutrition,2004,92(S1):S27-S30.

[33] RAY A K,GHOSH K,RINGØ E.Enzyme-producing bacteria isolated from fish gut:a review[J].Aquaculture Nutrition,2012,18(5):465-492.  

[34] 杨威,樊启学,宗克金,等.鳡幼鱼对晶体氨基酸的利用效果及赖氨酸需求量的研究[J].动物营养学报,2012,24(7):1255-1263.

[35] NAZ M,TVRKMEN M.Changes in the digestive enzymes and hormones of gilthead seabream larvae (Sparus aurata,L.1758) fed on Artemia nauplii enriched with free lysine[J].Aquaculture International,2009,17(6):523-535.  

[36] LALLÈS J P.Intestinal alkaline phosphatase:novel functions and protective effects[J].Nutrition Reviews,2014,72(2):82-94.  

[37] 张继平,林建成,谢进金,等.草鱼碱性磷酸酶的分离纯化与部分性质研究[J].厦门大学学报:自然科学版,2005,44(5):684-687.

[38] YUAN J,FENG L,JIANG W D,et al.Effects of dietary vitamin K levels on growth performance,enzyme activities and antioxidant status in the hepatopancreas and intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture Nutrition,2016,22(2):352-366.  

[39] ALMANSA E,SANCHEZ J,COZZI S,et al.Segmental heterogeneity in the biochemical properties of the Na+-K+-ATPase along the intestine of the gilthead seabream (Sparus aurata L.)[J].Journal of Comparative Physiology B,2001,171(7):557-567.  

[40] KOVACS-NOLAN J,RUPA P,MATSUI T,et al.In Vitro and ex vivo uptake of glutathione (GSH) across the intestinal epithelium and fate of oral GSH after in vivo supplementation[J].Journal of Agricultural and Food Chemistry,2014,62(39):9499-9506.  

[41] ABDEL-TAWWAB M,AHMAD M H,KHATTAB Y A E,et al.Effect of dietary protein level,initial body weight,and their interaction on the growth,feed utilization,and physiological alterations of Nile tilapia,Oreochromis niloticus (L.)[J].Aquaculture,2010,298(3/4):267-274.

[42] JIN M,WANG M Q,HUO Y W,et al.Dietary lysine requiremen
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