饲料营养 Feed science and technology

苜蓿皂苷对断奶仔猪生长性能、肠道菌群、组织抗氧化能力及相关酶mRNA表达的影响

展开
  • 河南农业大学牧医工程学院, 郑州 450002
王文静(1994-),女,河南商丘人,硕士研究生,从事牧草营养与利用研究。E-mail:2650220312@qq.com

收稿日期: 2017-05-18

  网络出版日期: 2017-12-04

基金资助

苜蓿品种引进与中低产田土壤生态环境修复(2015-Z46);现代农业产业技术体系(CARS-35)

Effects of Alfalfa Saponin on Growth Performance, Intestinal Microflora, Antioxidant Ability and Related Enzyme mRNA Expression in Tissues of Weaned Piglets

Expand
  • College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2017-05-18

  Online published: 2017-12-04

摘要

本试验旨在研究苜蓿皂苷对断奶仔猪生长性能、肠道菌群、组织抗氧化能力及相关酶mRNA表达的影响。选取24头平均体重为8 kg的大×长杂交断奶仔猪,随机分为2组,每组3个重复,每个重复4头猪。对照组饲喂基础饲粮,苜蓿皂苷组饲喂在基础饲粮中添加0.25%苜蓿皂苷的饲粮。预试期10 d,正试期30 d。结果表明:1)与对照组相比,饲粮中添加苜蓿皂苷可显著提高断奶仔猪平均日增重(P<0.05),并显著降低料重比(P<0.05)。2)与对照组相比,饲粮中添加苜蓿皂苷可显著降低仔猪十二指肠和盲肠的pH(P<0.05),显著提高十二指肠、空肠和回肠中乳酸菌数量(P<0.05)。3)与对照组相比,饲粮中添加苜蓿皂苷可显著提高仔猪肝脏和肾脏中谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性(P<0.05),并显著提高仔猪肝脏和空肠中GSH-Px mRNA表达量及十二指肠和回肠中CAT mRNA表达量(P<0.05)。综上,苜蓿皂苷可以提高断奶仔猪生长性能,增强其组织抗氧化能力并有效改善其肠道菌群。

本文引用格式

王文静, 刘伯帅, 陈要鹏, 孙骁, 肖俊楠, 林锦翔, 王成章, 史莹华 . 苜蓿皂苷对断奶仔猪生长性能、肠道菌群、组织抗氧化能力及相关酶mRNA表达的影响[J]. 动物营养学报, 2017 , 29(12) : 4469 -4476 . DOI: 10.3969/j.issn.1006-267x.2017.12.027

Abstract

The experiment was conducted to study to the effects of alfalfa saponin on growth performance, intestinal microflora, antioxidant ability and related enzyme mRNA expression in tissues of weaned piglets. A total of 24 crossed-bred (Landrace×Large White) piglets with an average body weight of 8 kg were randomly assigned to 2 groups with 3 replicates each and 4 piglets in each replicate. The piglets in control group were fed a basal diet, and in alfalfa saponin group were fed the basal diet supplemented with 0.25% alfalfa saponin. The experiment lasted for 30 days after 10 days pre-experiment. The results showed as follows:1) compared with control group, dietary alfalfa saponin significantly increased average daily gain (P<0.05) and significantly decreased the ratio of feed to gain (P<0.05) of weaned piglets. 2) Compared with control group, dietary alfalfa saponin significantly reduced pH in duodenum and cecum (P<0.05), significantly increased the numbers of Lactobacillus in duodenum, jejunum and cecum (P<0.05) of piglets. 3) Compared with control group, dietary alfalfa saponin significantly increased the activity of glutathion peroxidase (GSH-Px) and catalase (CAT) in liver and kidney (P<0.05), and significantly improved the mRNA expression level of GSH-Px in liver and jejunum as well as the mRNA expression level of CAT in duodenum and ileum (P<0.05) of piglets. In summary, dietary alfalfa saponin can enhance growth performance of weaned piglets, improve antioxidant ability in tissues and have a good benefit on intestinal microflora.

参考文献

[1] 武志敏,耿忠诚,王秀娜,等.甜菜碱和苜蓿皂甙对断奶仔猪生长性能的影响[J].黑龙江八一农垦大学学报,2010,22(3):58-60.

[2] YALINKILIC O,ENGINAR H.Effect of X-radiation on lipid peroxidation and antioxidant systems in rats treated with saponin-containing compounds[J].Photochemistry and Photobiology,2008,84(1):236-242.

[3] 徐兵.苜蓿皂甙对断奶仔猪的生长性能、消化生理和免疫的影响及机理的研究[D].硕士学位论文.郑州:河南农业大学,2008.

[4] 曹亚男.仔猪日粮中添加紫花苜蓿草粉的增重试验[J].山东畜牧兽医,2008,29(11):8.

[5] 侯永刚,陈辉,黄仁录,等.苜蓿皂甙对蛋鸡生产性能、屠体指标、胆固醇及血清脂质的影响[J].中国畜牧杂志,2009,45(17):30-33.

[6] 王彦华,王成章,高永革,等.苜蓿皂苷对断奶仔猪生产性能、消化率及血液生化指标的影响[J].草业学报,2009,18(5):115-122.

[7] LUPTON J R,JACOBS L R.Fiber supplementation results in expanded proliferative zones in rat gastric mucosa[J].The American Journal of Clinical Nutrition,1987,46(6):980-984.

[8] KASHIWAGURA T,DEUTSCH C J,TAYLOR J,et al.Dependence of gluconeogenesis,urea synthesis,and energy metabolism of hepatocytes on intracellular pH[J].Journal of Biological Chemistry,1984,259(1):237-243.

[9] SARRAZIN E,FRITZSCHE C,BERTSCHINGER H U.Main virulence factors in Escherichia coli isolates from swine over two weeks old with edema disease and/or E. coli diarrhea[J].Schweizer Archiv für Tierheilkunde,2000,142(11):625-630.  

[10] SERVIN A L.Antagonistic activities of Lactobacilli and Bifidobacteria against microbial pathogens[J].FEMS Microbiology Reviews,2004,28(4):405-440.  

[11] 彭茜,徐荣,殷宁,等.皂荚皂苷与茶皂苷抗菌活性研究[C]//林业生物质化学与工程学术研讨会论文集.南京:中国林学会,2009.

[12] 禹智辉,丁学知,夏立秋,等.藠头总皂苷抗菌活性及其作用机理[J].食品科学,2013,34(15):75-80.

[13] 金秋,奚立民,张昕欣,等.无患子皂苷的抗菌活性研究[J].科技通报,2014,30(3):35-38.

[14] 其买古丽·阿沙木,玛丽亚木·阿矾里米提,米丽班·霍加艾合买提,等.野蔷薇根中总皂苷的提取及其抑菌作用[J].光谱实验室,2013,30(6):3045-3051.

[15] 高清云,沈亮亮,施伟民,等.Bp3抗耐氟康唑白念珠菌活性研究[J].同济大学学报:医学版,2009,30(1):40-43.

[16] 李波,孙天颖,于鑫洋.苜蓿皂苷的抑菌活性和抗氧化特性的研究[J].食品研究与开发,2013(2):1-2,33.

[17] 武志敏.甜菜碱和苜蓿皂甙对断奶仔猪生长性能及免疫功能的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2010.

[18] RUDNEVA I I.Blood antioxidant system of Black Sea elasmobranch and teleosts[J].Comparative Biochemistry and Physiology Part C:Pharmacology,Toxicology and Endocrinology,1997,118(2):255-260.  

[19] MITTAL C K,MURAD F.Activation of guanylate cyclase by superoxide dismutase and hydroxyl radical:a physiological regulator of guanosine 3',5'-monophosphate formation[J].Proceedings of the National Academy of Sciences of the United States of America,1977,74(10):4360-4364.  

[20] 虞泽鹏,乐国伟,施用晖,等.不同锌源对断奶小鼠生长及机体抗氧化能力的影响[J].畜牧与兽医,2005,37(4):1-3.

[21] 刘文斐,刘伟龙,占秀安,等.不同形式蛋氨酸对肉种鸡生产性能、免疫指标及抗氧化功能的影响[J].动物营养学报,2013,25(9):2118-2125.

[22] SOYLU E M,SOYLU S,KURT S.Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans[J].Mycopathologia,2006,161(2):119-128.  

[23] 张金龙,徐丽丽,李艳飞.雏鸡脑软化症免疫组织的氧化和抗氧化特性[J].中国畜牧兽医,2007,34(2):42-44.

[24] 张晶晶,石少慧,巴伟松,等.银杏叶提取物对肾急性缺血再灌注损伤家兔抗氧化能力的影响[J].医学研究与教育,2005,22(1):4-5.

[25] SPARG S G,LIGHT M E,VAN STADEN J.Biological activities and distribution of plant saponins[J].Journal of Ethnopharmacology,2004,94(2/3):219-243.

[26] 刘宝剑,王存连,徐明举,等.人参皂苷Rb1对H9N2流感病毒诱导急性肺损伤小鼠抗氧化酶的影响[J].中国实验动物学报,2014,22(1):11,38-43.

[27] 史莹华,王成章,徐兵,等.苜蓿皂甙对断奶仔猪生长性能和抗氧化性能的影响[J].草地学报,2010,18(5):735-739.

[28] 孙延芳,梁宗锁,刘政,等.酸枣果三萜皂苷抑菌和抗氧化活性的研究[J].食品工业科技,2012,33(6):139-142.

[29] 管福琴,刘敏,单宇,等.灰毡毛忍冬中五环三萜类化合物的抗氧化活性研究[J].时珍国医国药,2013,24(6):1315-1317.

[30] 黄玉珊,曾靖,黄玉萍,等.大豆苷元对大鼠心、脑、肝组织中SOD和MDA的影响[J].华西药学杂志,2007,22(2):138-139.
文章导航

/