饲粮添加壳寡糖对肉鸡肉品质、抗氧化性能、小肠黏膜组织结构及肠道菌群的影响

白洁, 刘国华, 年芳, 郑爱娟, 蔡辉益, 常文环

doi:10.3969/j.issn.1006-267x.2017.12.029

动物营养学报 >

2017 , Vol. 29 >Issue 12: 4485 - 4494

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.029

饲料营养 Feed science and technology

饲粮添加壳寡糖对肉鸡肉品质、抗氧化性能、小肠黏膜组织结构及肠道菌群的影响

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1. 甘肃农业大学动物科学技术学院, 兰州 730070;
2. 中国农业科学院饲料研究所, 农业部生物饲料重点实验室, 北京 100081;
3. 甘肃农业大学理学院, 兰州 730070

白洁(1992-),女,甘肃兰州人,硕士研究生,从事单胃动物营养与饲料科学研究。E-mail:892062342@qq.com

收稿日期: 2017-06-02

网络出版日期: 2017-12-04

基金资助

现代农业产业技术体系北京市家禽创新团队项目；现代农业产业技术体系国家肉鸡产业技术体系项目（CARS-42）

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Effects of Dietary Chitosan Oligosaccharide on Meat Quality, Antioxidant Activity, Intestinal Mucosal Structure and Intestinal Flora of Broilers

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1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Key Laboratory of Feed Biotechnology of Agricultural Ministry, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;
3. Gansu Agricultural University of Natural Sciences Introduction, Lanzhou 730070, China

Received date: 2017-06-02

Online published: 2017-12-04

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摘要

本试验旨在研究饲粮添加壳寡糖（COS）对肉鸡肉品质、抗氧化性能、小肠黏膜组织结构及肠道菌群的影响。选用300只1日龄健康的爱拔益加（AA）雄性肉鸡，随机分为5组，每组6个重复，每个重复10只鸡。空白对照组饲喂不含抗生素的基础饲粮，正对照组饲喂添加100 mg/kg金霉素的基础饲粮，试验组分别饲喂添加50、100和150 mg/kg COS的基础饲粮。试验期42 d。结果表明，与空白对照组相比：1）饲粮添加100 mg/kg COS显著提高了胸肌和腿肌的红度（a^*）值（P<0.05）。2）饲粮添加50和100 mg/kg COS显著提高了1~21日龄血清总超氧化物歧化酶（T-SOD）活性（P<0.05），饲粮添加100 mg/kg COS显著提高了22~42日龄血清总抗氧化能力（T-AOC）和T-SOD、谷胱甘肽过氧化物酶（GSH-Px）活性（P<0.05），饲粮添加50 mg/kg COS显著提高了22~42日龄血清T-SOD和GSH-Px活性（P<0.05）。3）饲粮添加100 mg/kg COS显著提高了1~21日龄和22~42日龄空肠绒毛高度和绒毛高度/隐窝深度（P<0.05）。4）饲粮添加50和100 mg/kg COS显著降低了回肠大肠杆菌数量（P<0.05）。5）饲粮添加100 mg/kg COS显著提高了肉鸡的平均日增重（P<0.05）。由此可见，饲粮添加COS可提高肉鸡抗氧化性能，改善肉鸡肉品质和肠黏膜组织结构，并在一定程度上改善肠道菌群结构。COS在肉鸡饲粮中的适宜添加量为100 mg/kg。

关键词： 壳寡糖; 肉鸡; 抗氧化; 小肠黏膜组织结构; 肠道菌群

本文引用格式

白洁, 刘国华, 年芳, 郑爱娟, 蔡辉益, 常文环 . 饲粮添加壳寡糖对肉鸡肉品质、抗氧化性能、小肠黏膜组织结构及肠道菌群的影响[J]. 动物营养学报, 2017 , 29(12) : 4485 -4494 . DOI: 10.3969/j.issn.1006-267x.2017.12.029

Abstract

This experiment was conducted to study the effect of dietary chitosan oligosaccharide (COS) on meat quality, antioxidant activity, intestinal mucosal structure and intestinal flora of broilers. A total of 300 one-day-old healthy Arbor Acres (AA) male broilers were randomly allotted to 5 groups with 6 replicates per group and 10 birds per replicate. The blank control group was fed a basal diet without any antibiotic, the positive control group was fed the basal diet supplemented with 100 mg/kg chlortetracycline, and the experimental groups were fed the basal diet supplemented with 50, 100 and 150 mg/kg COS, respectively. The experiment lasted for 42 days. The results showed as follows, compared with the blank control group:1) dietary supplemented with 100 mg/kg COS significantly increased the redness (a^*) value of breast muscle and thigh muscle (P<0.05). 2) Dietary supplemented with 50 and 100 mg/kg COS significantly increased the serum total superoxide dismutase (T-SOD) activity during 1 to 21 days of age (P<0.05), dietary supplemented with 100 mg/kg of COS significantly increased the total antioxidant capacity (T-AOC), T-SOD activity and glutathione peroxides (GSH-Px) activity in serum during 22 to 42 days of age (P<0.05), dietary supplemented with 50 mg/kg COS significantly increased serum T-SOD and GSH-Px activities during 22 to 42 days of age (P<0.05). 3) Dietary supplemented with 100 mg/kg COS significantly increased the villous height and villous height/crypt depth of jejunum during 1 to 21 days of age and 22 to 42 days of age (P<0.05). 4) Dietary supplemented with 50 and 100 mg/kg COS significantly decreased the ileal Escherichia coli number (P<0.05). 5) Dietary supplemented with 100 mg/kg COS significantly increased the average daily gain of broilers (P<0.05). It can be concluded that dietary COS can increase antioxidant activity, improve meat quality and intestinal mucosal structure, and improve the intestinal flora structure to a certain extent of broilers. The optimum supplemental level of COS in diet for broilers is 100 mg/kg.

Key words： chitosan oligosaccharide; broilers; antioxidation; intestinal mucosal structure; intestinal flora

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