玉米赤霉烯酮对断奶小母猪卵巢指数及孕激素受体分布和表达的影响

杨立杰, 黄丽波, 李思梦, 刘法孝, 姜淑贞, 杨在宾

doi:10.3969/j.issn.1006-267x.2017.12.032

动物营养学报 >

2017 , Vol. 29 >Issue 12: 4510 - 4517

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.032

饲料安全 Feed Security

玉米赤霉烯酮对断奶小母猪卵巢指数及孕激素受体分布和表达的影响

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山东农业大学动物科技学院, 山东省动物生物工程与疾病防治重点实验室, 泰安 271018

杨立杰(1992-),男,山东潍坊人,硕士研究生,动物营养与饲料科学专业。E-mail:724832205@qq.com

收稿日期: 2017-06-02

网络出版日期: 2017-12-04

基金资助

国家自然科学基金资助项目（31572441）；山东省现代农业产业技术体系生猪创新团队建设项目（SDAIT-08-04）；"双一流"奖补资金资助

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Effects of Zearalenone on Ovary Index, Distribution and Expression of Progesterone Receptors in Ovaries of Weaned Gilts

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College of Animal Science and Technology, Shandong Agricultural University, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China

Received date: 2017-06-02

Online published: 2017-12-04

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摘要

本试验旨在研究不同水平玉米赤霉烯酮（ZEA）污染饲粮对断奶小母猪卵巢指数及孕激素受体的阳性分布和mRNA相对表达量的影响。选择40头35~38日龄健康三元杂交（杜×长×大）断奶小母猪，按平均体重[（14.01±0.86）kg]随机分为4个组，采用单笼饲养。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加0.5、1.0和1.5 mg/kg的ZEA，4个组ZEA的测定值分别为0、（0.52±0.07）mg/kg、（1.04±0.03）mg/kg和（1.51±0.13）mg/kg。试验期35 d。结果表明：随着饲粮ZEA水平的升高，断奶小母猪卵巢指数呈一次线性升高（P<0.01）；1.0 mg/kg ZEA组的卵巢指数显著高于1.5 mg/kg ZEA组（P<0.05），1.5 mg/kg ZEA组的卵巢指数显著高于0.5 mg/kg ZEA组和对照组（P<0.05）。随着饲粮ZEA水平的升高，断奶小母猪卵巢孕激素受体累积光密度（IOD）和mRNA相对表达量均呈一次线性升高（P<0.01）；1.5 mg/kg ZEA组的孕激素受体IOD和mRNA相对表达量显著高于1.0 mg/kg ZEA组（P<0.05），1.0 mg/kg ZEA组又显著高于0.5 mg/kg ZEA组和对照组（P<0.05）。免疫组化结果显示断奶小母猪卵巢中孕激素受体免疫阳性物质主要分布于原始卵泡和生长卵泡的卵母细胞及颗粒细胞、卵泡膜细胞及血管壁细胞。随着饲粮ZEA水平的升高和卵泡闭锁程度的增加，免疫阳性反应明显增强，但卵巢内孕激素受体免疫阳性物质的分布位置没有因ZEA的水平发生明显改变。由此可见，ZEA（1.0~1.5 mg/kg）可以通过调控断奶小母猪卵巢中孕激素受体的高水平表达，使孕激素受体的水平超出正向调节卵泡生长的限值，从而促使卵泡闭锁，降低卵巢指数，改变卵巢发育，进而影响断奶小母猪生殖系统的健康。

关键词： 玉米赤霉烯酮; 断奶小母猪; 卵巢; 孕激素受体

本文引用格式

杨立杰, 黄丽波, 李思梦, 刘法孝, 姜淑贞, 杨在宾 . 玉米赤霉烯酮对断奶小母猪卵巢指数及孕激素受体分布和表达的影响[J]. 动物营养学报, 2017 , 29(12) : 4510 -4517 . DOI: 10.3969/j.issn.1006-267x.2017.12.032

Abstract

The aims of the present study were to investigate the effects of zearalenone on ovary index, distribution and mRNA relative expression of progesterone receptors in ovaries of weaned gilts. Forty healthy weaned piglets (Duroc×Landrace×Large White) aged of 35 to 38 days were allocated into 4 groups according to the average body weight[(14.01 ±0.86) kg]. Animals were fed individually in a metabolic cage for 35 days. Piglets in the control group were fed a basal diet, and in experimental groups were fed basal diets supplemented with 0.5, 1.0 and 1.5 mg/kg ZEA, respectively. The analyzed ZEA levels in four groups were 0, (0.52±0.07) mg/kg, (1.04±0.03) mg/kg and (1.51±0.13) mg/kg. The results indicated that ovary index showed a linear increase (P<0.01) with the ZEA level increasing in the diet of weaned gilts. The ovary index in 1.0 mg/kg ZEA group was significantly higher than that in 1.5 mg/kg ZEA group (P<0.05), and the ovary index in 1.5 mg/kg ZEA group was significantly higher than that in 0.5 mg/kg ZEA group and the control group (P<0.05). The integrated optical density (IOD) and mRNA relative expression of progesterone receptors in ovaries of weaned gilts showed a linear increase (P<0.01) with the ZEA level increasing in the diet of weaned gilts. The IOD and mRNA relative expression of progesterone receptors in 1.5 mg/kg ZEA group was significantly higher than that in 1.0 mg/kg ZEA group, and the IOD and mRNA relative expression of progesterone receptors in 1.0 mg/kg ZEA group was significantly higher than that in 0.5 mg/kg ZEA group and the control group (P<0.05). The results of immunohistochemistry showed that the immunoreactive substance of progesterone receptors in ovaries was mainly distributed in oocytes and granulosa cells, theca cells and vascular wall cells of primordial follicles and growing follicles. With the increase of ZEA level and the degree of follicular atresia, immunoreactivity was significantly enhanced. There was no significant change in the distribution of immunoreactive substance of progesterone receptors in the ovary, due to the presence of ZEA. Thus it can be seen that ZEA (1.0 to 1.5 mg/kg) in feed changes the development of ovary by up-regulating the expression of progesterone receptors. The levels of progesterone receptors exceed the limit of positive regulation of follicular growth, thus promoting follicular atresia, reducing the ovary index, and then the health of reproductive system is affected.

Key words： zearalenone; weaned gilts; ovary; progesterone receptor

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