研究简报 Short communications

饲粮碘含量对牦牛体外瘤胃发酵的影响

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  • 1. 省部共建三江源生态与高原农牧业国家重点实验室, 青海省高原放牧家畜动物营养与饲料科学重点实验室, 西宁 810016;
    2. 青海高原牦牛研究中心, 西宁 810016;
    3. 青海大学畜牧兽医科学院, 西宁 810016
范小红(1992-),女,甘肃定西人,硕士研究生,研究方向为饲草料营养价值评价。E-mail:1642246477@qq.com

收稿日期: 2017-06-05

  网络出版日期: 2017-12-04

基金资助

国家自然科学基金(31660673);国家重点研发计划(2016YFD0500504-4);国家国际科技合作专项项目(2015DFG31870);国家重点基础研究发展计划(973项目)(2012CB722906);青海省重大科技平台建设项目(2013-Z-Y03)

Effects of Dietary Iodide Content on Rumen Fermentation of Yaks in Vitro

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  • 1. State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining 810016, China;
    2. Qinghai Plateau Yak Research Center, Xining 810016, China;
    3. Academy of Science and Veterinary Medicine of Qinghai University, Xining 810016, China

Received date: 2017-06-05

  Online published: 2017-12-04

摘要

本试验旨在研究饲粮碘含量对牦牛体外瘤胃发酵的影响。以碘化钾作为添加形式,以牦牛饲粮为底物进行体外发酵,底物碘含量分别为0.1、0.3、0.5、0.7、0.9 mg/kg,共发酵48 h。发酵结束测定总产气量、瘤胃发酵特性指标及消化酶活力。结果表明:当底物碘含量为0.3 mg/kg时,发酵液微生物蛋白质(MCP)、乙酸(C2)、丙酸(C3)、丁酸(C4)、异戊酸(P<0.05);在碘含量为0.7 mg/kg时,发酵液纤维素酶(CLS)活力达到最大值,为79.956 U/mL,发酵液乙酸/丙酸最低,为1.636。综合各项指标得出,在体外条件下,在底物碘含量为0.3~0.7 mg/kg时,牦牛体外瘤胃发酵处较高水平。

本文引用格式

范小红, 薛艳锋, 郝力壮, 刘书杰, 柴沙驼, 牛建章, 张晓卫, 王迅 . 饲粮碘含量对牦牛体外瘤胃发酵的影响[J]. 动物营养学报, 2017 , 29(12) : 4613 -4620 . DOI: 10.3969/j.issn.1006-267x.2017.12.044

Abstract

This study was conducted to investigate the effects of dietary iodide content on rumen fermentation of yaks in vitro. Potassium iodide was used as additive and diet for yaks was used as a substrate for in vitro rumen fermentation. Iodine content in substrate was designed as 0.1, 0.3, 0.5, 0.7 and 0.9 mg/kg, respectively. The fermentation lasted for 48 h. Gas production, rumen fermentation characteristic parameters and digestive enzyme activities were measured after fermentation. The results showed as follows:when the content of iodine in substrate was 0.3 mg/kg, the concentrations of microbial protein (MCP), acetic acid (C2), propionic acid (C3), butyric acid (C4), isovaleric acid (P<0.05); when the content of iodine in substrate was 0.7 mg/kg, cellulose (CLS) activity in fermentation fluid reached the highest, which was 79.956 U/mL, and C2/C3 in fermentation fluid reached the lowest, which was 1.636. Based on the various indexes, under in vitro conditions, when the content of iodine in substrate was 0.3 to 0.7 mg/kg, in vitro ruminal fermentation of yaks is at high level.

参考文献

[1] 郭宪,阎萍,梁春年,等.中国牦牛业发展现状及对策分析[J].中国牛业科学,2009,35(2):55-57.

[2] 朱新书,阎萍,梁春年,等.从生态学观点论青藏高原地区牦牛产业的可持续发展[J].黑龙江畜牧兽医,2011(3):148-150.

[3] 孟献亚,李亚楠,张秀丽,等.牦牛肉和牦牛乳中碘含量及甲状腺激素检测结果分析[J].现代预防医学,2017,44(10):1774-1775,1779.

[4] 韩兴泰,胡令浩,谢敖云,等.粗饲条件下生长牦牛能量代谢的估测[J].青海畜牧兽医杂志,1992,22(2):21-22.

[5] 薛白,柴沙驼,刘书杰,等.生长期牦牛蛋白质需要量的研究[J].青海畜牧兽医杂志,1994,24(4):1-5.

[6] NUDDA A,BATTACONE G,DECANDIA M,et al.The effect of dietary iodine supplementation in dairy goats on milk production traits and milk iodine content[J].Journal of Dairy Science,2009,92(10):5133-5138.  

[7] 孔祥瑞.必需微量元素的营养生理及临床意义[M].合肥:安徽科学技术出版社,1982:121.

[8] 孙全文.必需微量元素碘对家兔的营养作用[J].中国养兔,2009(5):34-39.

[9] SARGISON N D,WEST D M,CLARK R G.The effects of iodine deficiency on ewe fertility and perinatal lamb mortality[J].New Zealand Veterinary Journal,1998,46(2):72-75.  

[10] 李西峰,杨全明.必需微量元素碘[J].中国饲料,1995(20):31.

[11] 杨凤.动物营养学[M].2版.北京:中国农业出版社,2003:116-117.

[12] 郭洋,杜绍范.碘对于反刍动物的毒性[J].现代畜牧兽医,2005(7):52-53.

[13] 杨国忠,王净,孙泰然,等.微量元素碘对动物的营养作用[J].今日畜牧兽医,2007(1):52-53.

[14] NRC.Nutrient requirements of small ruminants:sheep,goats,cervids,and New World camelids[S].Washington,D.C.:National Academies Press,2007.

[15] 柴沙驼,刘书杰,谢敖云,等.生长期牦牛对饲料中氮利用率的研究[J].草食家畜,1996(4):36-39,57.

[16] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agricultural Science,1979,93(1):217-222.  

[17] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 13882-2010饲料中碘的测定硫氰酸铁-亚硝酸催化动力学法[S].北京:中国标准出版社,2011.

[18] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(4):40-41.

[19] 曹庆云,周武艺,朱贵钊,等.气相色谱测定羊瘤胃液中挥发性脂肪酸方法研究[J].中国饲料,2006(24):26-28.

[20] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2012:139-141.

[21] MENKE K H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.

[22] 哈斯花.体外发酵产气技术在饲料营养价值评定中的作用[J].当代畜禽养殖业,2013(1):15-19.

[23] 王茂荣.不同水平的铜、铁、碘、钼对RSI-Ⅱ人工瘤胃微生物发酵的影响[D].硕士学位论文.晋中:山西农业大学,2001.

[24] 高天爽,孙海霞,谢小来,等.日粮中高粱替代玉米对绵羊瘤胃发酵的影响[J].黑龙江畜牧兽医,2014(9):7-10.

[25] WALES W J,KOLVER E S,THORNE P L,et al.Diurnal variation in ruminal pH on the digestibility of highly digestible perennial ryegrass during continuous culture fermentation[J].Journal of Dairy Science,2004,87(6):1864-1871.  

[26] KOPECNY J,WALLACE R J.Cellular location and some properties of proteolytic enzymes of rumen bacteria[J].Applied and Environmental Microbiology,1982,43(5):1026-1033.

[27] 陈光吉,严锦绣,郭春华,等.微生物发酵饲料对肉牛瘤胃液pH值和微生物数量的影响[J].黑龙江畜牧兽医,2015(7):104-107.

[28] 张海涛,王加启,卜登攀,等.纳豆枯草芽孢杆菌对犊牛断奶前后瘤胃发酵和酶活的影响[J].中国畜牧兽医,2009,36(12):5-11.

[29] 刘彩娟,孙满吉,孙金艳,等.饲粮中添加复合益生菌对奶牛瘤胃发酵及纤维素酶活的影响[J].动物营养学报,2011,23(5):821-827.

[30] 王杰.小尾寒羊、滩羊生长性能及其主要消化生理参数的比较[D].硕士学位论文.杨凌:西北农林科技大学,2008.

[31] LENG R A,NOLAN J V.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,1984,67(5):1072-1089.  

[32] MURPHY J J,KENELLY J J.Effect of protein concentration and protein source on the degradability of dry matter and protein in situ[J].Journal of Dairy Science,1987,70(9):1841-1849.  

[33] SLYTER L L.Monensin and dichloroacetamide influences on methane and volatile fatty acid production by rumen bacteria in vitro[J].Applied and Environmental Microbiology,1979,37(2):283-288.

[34] GRAY G M.Starch digestion and absorption in nonruminants[J].The Journal of Nutrition,1992,122(1):172-177.
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