动物营养学报 >

2018 , Vol. 30 >Issue 2: 515 - 523

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.015

猪营养 Swine Nutrition

亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响

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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
李先根(1993-),男,湖北十堰人,硕士研究生,从事猪的营养与免疫的研究。E-mail:823052910@qq.com

收稿日期: 2017-08-21

  网络出版日期: 2018-02-02

基金资助

湖北省教育厅优秀中青年科技创新团队项目（T201508）

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Effects of Flaxseed Oil on Intestinal Mucosa Structure and Immune Cells of Weaned Piglets after Lipopolysaccharide Challenge

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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2017-08-21

  Online published: 2018-02-02

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摘要

本试验旨在研究亚麻籽油对脂多糖（LPS）刺激断奶仔猪肠黏膜结构和免疫细胞的影响。选择24头（9.15±0.90）kg杜×长×大断奶仔猪，随机分为4组，每组6个重复，每个重复1头猪。试验采用2×2双因子设计，主因子包括：1）饲粮处理（5%玉米油或5%亚麻籽油）；2）免疫应激（注射生理盐水或LPS）。饲粮中添加5%玉米油或5%亚麻籽油饲喂21 d，在试验第21天，每组1/2的猪腹膜注射100 μg/kg BW的LPS，另外1/2的猪腹膜注射等量生理盐水。注射LPS或生理盐水4 h后，将仔猪麻醉屠宰，取肠道样品待测。结果表明：1）LPS刺激显著降低了空肠、回肠绒毛高度和空肠隐窝深度（P<0.05），显著提高空肠固有层细胞密度和嗜中性粒细胞数目（P<0.05），有降低空肠上皮间淋巴细胞数目（P=0.080）和回肠杯状细胞数目（P=0.059）的趋势；2）LPS刺激对亚麻籽油组仔猪空肠、回肠绒毛高度无显著影响（P>0.05）；饲粮添加5%亚麻籽油有提高回肠派氏结细胞密度的趋势（P＝0.064），且在LPS刺激下，显著降低空肠固有层细胞密度（P<0.05），并有增加空肠上皮间淋巴细胞数目的趋势（P＝0.069）。结果提示，5%亚麻籽油在一定程度上可维护LPS刺激断奶仔猪的小肠黏膜结构和免疫功能。

关键词： 亚麻籽油; 断奶仔猪; 脂多糖; 肠黏膜免疫细胞

本文引用格式

李先根, 涂治骁, 王树辉, 王海波, 张琳, 秦琴, 汪龙梅, 王秀英, 刘玉兰, 朱惠玲 . 亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响[J]. 动物营养学报, 2018 , 30(2) : 515 -523 . DOI: 10.3969/j.issn.1006-267x.2018.02.015

Abstract

The purpose of this study was to investigate the effects of flaxseed oil on intestinal mucosa structure and immune cells of weaned piglets after lipopolysaccharide (LPS) challenge. Twenty-four Duroc×Landrace×Yorkshine crossed-bred weaned piglets with average body weight (BW) of (9.15±0.90) kg were randomly divided into four groups with six replicates each and one pig per replicate. The experiment consisted of a 2×2 factorial design, and the main factors included:1) diet treatment (5% flaxseed oil or 5% corn oil); 2) immunological challenge (LPS or physiological saline injection). After being fed diets supplemented with 5% corn oil or 5% flaxseed oil for 21 days, pigs were injected intraperitoneally with 100 μg/kg BW LPS or the same doze of saline half and half. At 4 h postinjection, pigs were anaesthetized and slaughtered, and the intestinal samples were collected for analysis. The results showed as follows:1) LPS challenge significantly decreased villus height in jejunum and ileum as well as crypt depth in jejunum (P<0.05), and significantly increased the lamina propria cell density and the number of neutrophils in jejunum (P<0.05). LPS challenge tended to reduce the number of epithelial lymphocytes (IELs) in jejunum (P=0.080)and the number of goblet cells in ileum (P=0.059). 2) LPS challenge did not significantly affect villus height in jejunum and ileum of pigs in flaxseed oil groups (P>0.05). However, dietary 5% flaxseed oil tended to increase the Peyer's patch cell density in ileum (P=0.064), especially under LPS challenge, significantly reduced the lamina propria cell density in jejunum (P<0.05), and tended to increase the number of IELs in jejunum (P=0.069). These results indicate that 5% flaxseed oil can maintain small intestinal mucosa structure and immune function of weaned piglets after LPS challenge.

Key words： flaxseed oil; weaned piglets; lipopolysaccharide; intestinal mucosa immune cells

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