动物营养学报 >

2018 , Vol. 30 >Issue 2: 667 - 677

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.032

饲料营养 Feed science and technology

吡咯喹啉醌二钠对产蛋鸡生产性能、抗氧化状态和血浆生化指标的影响

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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 生物饲料开发国家工程研究中心, 北京 100081
王晶(1984-),女,河南鹤壁人,博士研究生,从事家禽营养调控方面的研究。E-mail:wangjing@caas.cn

收稿日期: 2017-08-15

  网络出版日期: 2018-02-02

基金资助

国家蛋鸡产业技术体系项目（CARS-41-K13）；国家自然科学基金项目（31172212）

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Effects of Pyrroloquinoline Quinone Disodium on Performance, Antioxidant Status and Plasma Biochemical Parameters of Laying Hens

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  • National Engineering Research Center of Biological Feed, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-08-15

  Online published: 2018-02-02

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摘要

本试验通过研究不同添加量的吡咯喹啉醌二钠（PQQ·Na2）对产蛋鸡生产性能、抗氧化状态和血浆生化指标的影响，旨在评价PQQ·Na2在产蛋鸡上的生物学安全性。试验选用540只25周龄的健康海兰灰蛋鸡，随机分为5组，每组设6个重复，每个重复18只。5组产蛋鸡分别饲喂添加0（对照）、0.04、0.08、0.12 mg/kg PQQ·Na2及200 mg/kg维生素E的5种试验饲粮。预试期1周，正试期24周。结果表明：1）与对照组相比，饲粮添加0.04~0.12 mg/kg PQQ·Na2对产蛋鸡的生产性能未产生显著影响（P>0.05）；2）试验第24周，与对照组相比，饲粮添加0.04~0.12 mg/kg PQQ·Na2显著提高了鸡蛋的哈氏单位（P<0.05），且添加0.08 mg/kg PQQ·Na2还显著提高了蛋壳厚度（P<0.05）；3）与对照组相比，饲粮添加0.08和0.12 mg/kg PQQ·Na2显著提高了血浆谷胱甘肽过氧化物酶（第12周）和总超氧化物歧化酶活性（第2周、第24周）（P<0.05）；4）与对照组相比，饲粮添加0.08 mg/kg PQQ·Na2显著降低了心脏羰基含量（P<0.05），显著提高了肝脏总抗氧化能力（P<0.05），且添加0.12 mg/kg PQQ·Na2还显著降低了肝脏丙二醛含量（P<0.05）。上述试验结果提示，饲粮添加0.08~0.12 mg/kg PQQ·Na2对产蛋鸡的生产性能无不良影响，且可改善机体抗氧化功能，综合考虑得出产蛋鸡饲粮中PQQ·Na2的适宜添加量为0.08 mg/kg。

关键词： 吡咯喹啉醌二钠; 产蛋鸡; 抗氧化; 血浆生化指标; 蛋品质

本文引用格式

王晶, 杨林林, 张海军, 武书庚, 齐广海 . 吡咯喹啉醌二钠对产蛋鸡生产性能、抗氧化状态和血浆生化指标的影响[J]. 动物营养学报, 2018 , 30(2) : 667 -677 . DOI: 10.3969/j.issn.1006-267x.2018.02.032

Abstract

The current study aimed to evaluate the biological safety of pyrroloquinoline quinone disodium (PQQ·Na2) for laying hens and investigated the effects of dietary PQQ·Na2 suplemental levels on performance, antioxidant status and plasma biochemical parameters. Five hundred and forty 25-week-old Hyline Grey laying hens were randomly divided into 5 groups with each group consisted of 6 replicates of 18 birds each. The laying hens in the 5 groups were fed 5 experimental diets with 0 (control), 0.04, 0.08, 0.12 mg/kg PQQ·Na2 and 200 mg/kg vitamin E, respectively. All hens were fed the diets for a 1-week adaptation followed by a 24-week trial period. The result showed as follows:1) diet supplemented with 0.04 to 0.12 mg/kg PQQ·Na2 did not affect the performance of laying hens (P>0.05). 2) Compared with the control group, the Haugh unit was significantly increased by diet supplemented with 0.04 to 0.12 mg/kg PQQ·Na2 (P<0.05), and the eggshell thickness was significantly increased by diet supplemented with 0.08 mg/kg PQQ·Na2 at the week 24 of trial (P<0.05). 3) Compared with the control group, the activities of plasma glutathion peroxidase (GSH-Px) (week 12) and total superoxide dismutase (T-SOD) (week 2 and 24) were significantly increased by diet supplemented with 0.08 and 0.12 mg/kg PQQ·Na2 (P<0.05). 4) Compared with the control group, diet supplemented with PQQ·Na2 at 0.08 mg/kg significantly decreased the content of heart carbonyl group and significantly increased the hepatic total antioxidant capacity (T-AOC) (P<0.05), and diet supplemented with 0.12 mg/kg PQQ·Na2 significantly decreased the hepatic malondialdehyde (MDA) content (P<0.05). Above results indicate that the supplementation of PQQ·Na2 at 0.08 to 0.12 mg/kg in laying hens' diet do not have negative effects, but can enhance the laying hens' antioxidant function. The suitable supplemental level of PQQ·Na2 in laying hens' diet is 0.08 mg/kg by comprehensive considering of the influence factors.

Key words： pyrroloquinoline quinone disodium; laying hens; antioxidant; plasma biochemical parameters; egg quality

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