饲粮中添加肠杆菌肽对断奶仔猪生产性能和血清生化指标的影响

卜艳玲, 陈静, 李建涛, 程奇, 李海东, 刘显军

doi:10.3969/j.issn.1006-267x.2018.02.035

动物营养学报 >

2018 , Vol. 30 >Issue 2: 696 - 706

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.035

饲料营养 Feed science and technology

饲粮中添加肠杆菌肽对断奶仔猪生产性能和血清生化指标的影响

展开

1. 沈阳农业大学畜牧兽医学院, 沈阳 110866;
2. 沈阳博睿农牧科技有限公司, 沈阳 110300

卜艳玲(1988-),女,辽宁营口人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:675214566@qq.com

收稿日期: 2017-08-27

网络出版日期: 2018-02-02

基金资助

辽宁省科技厅重大项目"昌图瘦肉型猪选育及标准化健康养殖产业化示范基地"（2014215031）；辽宁省科技厅重大项目"昌图瘦肉型猪选育及标准化健康养殖产业化示范基地"（2015103034）

收起

Effects of Enterobacitracin Supplementation on Performance and Serum Biochemical Indices of Weaning Piglets

Expand

1. College of Animal Sciences and Veterinary Sciences, Shenyang Agricultural University, Shenyang 110866, China;
2. Shenyang Borui Agriculture and Animal Husbandry Technology Co., Ltd., Shenyang 110300, China

Received date: 2017-08-27

Online published: 2018-02-02

Fold

摘要

本试验旨在研究肠杆菌肽在断奶仔猪生产中的应用效果。选取144头28日龄且平均体重为（7.35±0.21）kg的健康"杜×长×大"三元杂交断奶仔猪，随机分为6组，每组4个重复，每个重复6头猪（公母各占1/2）。对照组仔猪饲喂基础饲粮，试验组仔猪饲喂在基础饲粮基础上分别添加硫酸黏菌素（30 g/t）、天蚕素抗菌肽（300 g/t）以及肠杆菌肽（300、400和500 g/t）的试验饲粮。试验期为28 d，分为试验前期（第1~14天）和试验后期（第15~28天）2个阶段。结果显示：1）试验前期、试验后期和整个试验期，400和500 g/t肠杆菌肽组的平均日增重（ADG）均显著高于对照组（P<0.05），料重比均显著低于对照组（P<0.05）。试验第7、14和28天时，各试验组腹泻率均极显著低于对照组（P<0.01），其中以500 g/t肠杆菌肽组最低。2）试验第14天时，500 g/t肠杆菌肽组血清尿素氮（UN）含量显著低于对照组和30 g/t硫酸黏菌素组（P<0.05）；试验第28天时，400和500 g/t肠杆菌肽组血清UN含量显著低于对照组（P<0.05），且500 g/t肠杆菌肽组还显著低于30 g/t硫酸黏菌素组（P<0.05）。试验第14和28天时，400和500 g/t肠杆菌肽组血清球蛋白（GLB）含量显著高于对照组（P<0.05）。3）试验第14和28天时，500 g/t肠杆菌肽组血清免疫球蛋白G（IgG）含量较对照组分别高出17.27%（P<0.05）、16.20%（P<0.05）。试验第14天时，各抗菌肽组血清补体4（C4）含量均显著高于对照组和30 g/t硫酸黏菌素组（P<0.05）。4）试验第14和28天时，各抗菌肽组血清丙二醛（MDA）含量极显著低于对照组和30 g/t硫酸黏菌素组（P<0.01）。试验第28天时，500 g/t肠杆菌肽组血清超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）显著高于对照组和30 g/t硫酸黏菌素组（P<0.05）。综上所述，肠杆菌肽在提高断奶仔猪生产性能、免疫性能和抗氧化能力方面优于天蚕素抗菌肽，也优于抗生素硫酸黏菌素，饲粮中肠杆菌肽的适宜添加量为500 g/t。

关键词： 肠杆菌肽; 断奶仔猪; 生产性能; 免疫性能; 抗氧化能力

本文引用格式

卜艳玲, 陈静, 李建涛, 程奇, 李海东, 刘显军 . 饲粮中添加肠杆菌肽对断奶仔猪生产性能和血清生化指标的影响[J]. 动物营养学报, 2018 , 30(2) : 696 -706 . DOI: 10.3969/j.issn.1006-267x.2018.02.035

Abstract

This experiment was designed to study the application effect of enterobacitracin in the production of weaning piglets. One hundred and forty-four healthy piglets (Duroc×Landrace×Large White) at the age of 28 days and with the average body weight of (7.35±0.29) kg were randomly selected and allotted to six groups with four replicates of six piglets per replicate (3 males and 3 females). Piglets in control group were fed a basal diet, and piglets in experimental groups were fed experimental diets supplemented with colistin sulfate (30 g/t), cecropin (300 g/t) and enterobacitracin (300, 400 and 500 g/t), respectively. The test lasted for 28 days, including early period (day 1 to 14) and later period (day 15 to 28). The results showed as follows:1) compared with control group, the average daily gain (ADG) of 400 and 500 g/t enterobacitracin groups was significantly increased (P<0.05), and the feed to gain ratio (F/G) was significantly decreased (P<0.05). At the 7^th, 14^th and 28^th day, the diarrhea rate of experimental groups was significantly lower than that of control group (P<0.01), and the 500 g/t enterobacitracin group had the lowest value. 2) The serum urea nitrogen (UN) content of 500 g/t enterobacitracin group was significantly lower than that of control group and 30 g/t colistin sulfate group at the 14^th day (P<0.05), and the serum UN content of 400 and 500 g/t enterobacitracin groups was significantly lower than that of control group at the 28^th day (P<0.05); moreover, the serum UN content of 500 g/t enterobacitracin group was significantly lower than that of 30 g/t colistin sulfate group at the 28^th day (P<0.05). At the 14^th and 28^th day, the serum globulin (GLB) content of 400 and 500 g/t enterobacitracin groups was significantly higher than that of control group (P<0.05). 3) The serum immunoglobulin G (IgG) content of 500 g/t enterobacitracin group was increased by 13.63% (P<0.05) and 13.63% (P<0.05) compared with control group, respectively. The serum complement 4 (C4) content of antimicrobial peptide groups was significantly higher than that of control group and 30 g/t colistin sulfate group at the 14^th day (P<0.05). 4) At the 14^th and 28^th day, the serum (MDA) content of antimicrobial peptide groups was significantly lower than that of control group and 30 g/t colistin sulfate group (P<0.01). At the 28^th day, the serum superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) were significantly higher than those of control group and 30 g/t colistin sulfate group (P<0.05). In conclusion, enterobacitracin is superior to cecropin in improving performance,immune function and antioxidant capacity of weanling piglets, and also superior to the antibiotic of colistin sulfate, which is suitable for 500 g/t in the diet.

Key words： enterobacitracin; weanling piglets; performance; immune function; antioxidant capacity

参考文献

[1] YOON J H,INGALE S L,KIM K H,et al.Effects of dietary supplementation with antimicrobial peptide-P5 on growth performance,apparent total tract digestibility,faecal and intestinal microflora and intestinal morphology of weanling pigs[J].Journal of the Science of Food and Agriculture,2013,93(3):587-592.

[2] 魏媛,龚国利.抗菌肽与革兰氏阳性菌的互作及其机制[J].动物营养学报,2017,29(8):2636-2642.

[3] ZHU X,DONG N,WANG Z Y,et al.Design of imperfectly amphipathic α-helical antimicrobial peptides with enhanced cell selectivity[J].Acta Biomaterialia,2014,10(1):244-257.

[4] 杜淑环,刘秀明,万秋,等.天蚕素抗菌肽的研究进展[J].动物营养学报,2012,24(1):41-47.

[5] 莫丹,付超强,李萍萍,等.肠杆菌肽的研究进展与应用[J].北方牧业,2016(9):27-28.

[6] 单安山,徐博成,王家俊,等.细菌对抗菌肽的抗性及机理[J].动物营养学报,2017,29(6):1874-1883.

[7] CHOI H,RANGARAJAN N,WEISSHAAR J C.Lights,camera,action!Antimicrobial peptide mechanisms imaged in space and time[J].Trends in Microbiology,2016,24(2):111-122.

[8] 李希,张江,何涛.日粮中添加肠杆菌肽对保育猪生长性能的影响[J].饲料博览,2016(2):1-4.

[9] 宦海琳,白建勇,周维仁,等.抗菌肽对仔猪血清生化指标、肠黏膜形态结构及空肠上皮紧密连接蛋白基因相对表达量的影响[J].动物营养学报,2015,27(12):3797-3804.

[10] 王彩玲,郭效中,牛子清,等.黄霉素、硫酸粘杆菌素对生长猪生产性能的影响[J].饲料工业,2002,23(8):24-25.

[11] 张文飞,刘苹苹,管武太,等.饲粮中添加卵黄抗体对断奶仔猪生长性能、血清生化指标、肠道形态及肠道微生物菌群的影响[J].动物营养学报2017,29(1):271-279.

[12] 袁威,仁志华,邓又天,等.复合抗菌肽对断奶仔猪生长性能及血清细胞因子含量的影响[J].动物营养学报,2015,27(3):885-892.

[13] YOON J H,INGALE S L,KIM J S,et al.Effects of dietary supplementation of synthetic antimicrobial peptide-A3 and P5 on growth performance,apparent total tract digestibility of nutrients,fecal and intestinal microflora and intestinal morphology in weanling pigs[J].Livestock Science,2014,159:53-60.

[14] XIONG X,YANG H S,LI L,et al.Effects of antimicrobial peptides in nursery diets on growth performance of pigs reared on five different farms[J].Livestock Science,2014,167:206-210.

[15] 刘显军,丁雪,陈静,等.抗菌肽和抗生素对断奶仔猪生长性能的影响[J].中国畜牧兽医,2012,39(7):252-256.

[16] 李方方,苏航,张勇,等.饲粮添加复合抗菌肽与包被氧化锌对断奶仔猪生长性能及血清生化指标的影响[J].动物营养学报,2015,27(9):2811-2819.

[17] 朱宇旌,张勇,李方方,等.抗菌肽在断奶仔猪饲粮中的应用效果研究[J].养猪,2016(2):17-20.

[18] 王阿荣.天蚕素抗菌肽对断奶仔猪和母猪生产性能的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2011.

[19] INGALE S L,CHOI S C,KIM J S,et al.Effects of dietary supplementation with an antimicrobial peptide-P5 on growth performance,nutrient retention,excreta and intestinal microflora and intestinal morphology of broilers[J].Animal Feed Science and Technology,2013,185(1/2):78-84.

[20] WANG S,ZENG X F,YANG Q,et al.Antimicrobial peptides as potential alternatives to antibiotics in food animal industry[J].International Journal of Molecular Sciences,2016,17(5):603.

[21] 张江,关静姝,李成功.日粮中添加肠杆菌肽对断奶仔猪生产性能的影响[J].广东饲料,2016,25(5):21-23.

[22] 孙何军,张江,刘成宏,等.日粮中添加肠杆菌肽对蛋鸡产蛋性能的影响[J].饲料工业,2016,37(13):28-30.

[23] 张奕驰,睢富根.抗菌肽替代硫酸黏菌素对断奶仔猪生长性能和抗腹泻性能的影响[J].中国畜牧杂志,2017,53(2):132-134.

[24] MANGONI M L,BHUNIA A.Editorial:antimicrobial peptides in medicinal chemistry:advances and applications[J].Current Topics in Medicinal Chemistry,2016,16(1):2-3.

[25] 陈畅.中草药添加剂对断奶仔猪生产性能、血清生化指标、粪中菌群指标的影响[D].硕士学位论文.沈阳:沈阳农业大学,2017.

[26] 赵玉蓉,周灿,范志勇,等.断奶及日龄对仔猪骨髓组织抗菌肽Protegrin-1 mRNA表达的影响[J].动物营养学报,2007,19(5):605-609.

[27] 朱振鹏,孙晓先,牛俊伟.天蚕素抗菌肽在动物生产上的研究进展[J].广东饲料,2013,22(8):29-32.

[28] WU S D,ZHANG F R,HUANG Z M,et al.Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli[J].Peptides,2012,35(2):225-230.

[29] 孙雪梅,梁伟凡,陈春萍,等.天蚕素抗菌肽替代硫酸黏杆菌素对断奶仔猪生长性能及腹泻的影响[J].饲料工业,2017,38(4):12-15.

[30] 李波,韩文瑜.日粮中添加天蚕素抗菌肽对断奶仔猪生产性能、免疫性能及血清生化指标的影响[J].中国畜牧兽医,2014,41(7):99-103.

[31] 彭翔,孙全友,李杰,等.抗菌肽和姜黄素对1-21日龄肉仔鸡生长性能和免疫功能的影响[J].动物营养学报,2014,26(2):474-481.

[32] 顾宪红,张宏福.环境条件对断奶仔猪健康及生产性能的影响[J].动物营养学报,1999,11(4):9-16.

[33] 僧晓兰,李英伦,施飞,等.峨眉雪胆微丸对断奶仔猪免疫功能的影响[J].西北农林科技大学学报,2016,44(6):34-39.

[34] 邝哲师,杨金波,赵祥杰,等.猪源性PR39抗菌肽对断奶仔猪生产性能、血清免疫指标和肠道菌群的影响[J].动物营养学报,2014,26(6):1587-1592.

[35] 苏恺,王凯,孙力军,等.抗菌肽对断奶仔猪生产性能和健康水平的影响[J].河南农业科学,2013,42(9):112-115,127.

[36] YUAN W,JIN H T,REN Z H,et al.Effects of antibacterial peptide on humoral immunity in weaned piglets[J].Food and Agricultural Immunology,2015,26(5):682-689.

[37] 姚远,匡伟,黄忠阳,等.抗菌肽天蚕素对鸡生长性能、肠道黏膜形态、盲肠菌群及免疫功能的影响[J].江苏农业学报,2014,30(2):331-338.

[38] 刘洹兵,刘毅.抗菌肽在肉鸡日粮中添加效果研究[J].湖南畜牧兽医,2015(4):6-9.

[39] 李建喜,杨志强,王学智.活性氧自由基在动物机体内的生物学作用[J].动物医学进展,2006,27(10):33-36.

[40] 袁施彬,陈代文.氧化应激对断奶仔猪组织抗氧化酶活性和病理学变化的影响[J].中国兽医学报,2009,29(1):74-78.

[41] 徐建凯,单安山,李建平,等.女贞子对断奶仔猪生产性能及抗氧化功能的影响[J].东北农业大学学报,2010,41(4):76-81.

[42] 孙全友,徐彬,王琳燚,等.抗菌肽和姜黄素对肉仔鸡生长性能、抗氧化功能及肠道微生物的影响[J].中国家禽,2015,37(20):32-36.

[43] 但启雄,袁威,邓又天,等.复合抗菌肽对断奶仔猪血清抗氧化功能的影响[J].中国兽医学报,2015,35(5):805-808.

[44] XIAO H,WU M M,TAN B E,et al.Effects of composite antimicrobial peptides in weanling piglets challenged with deoxynivalenol:Ⅰ.Growth performance,immune function,and antioxidation capacity[J].American Society of Animal Science,2013,91(10):4772-4780.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们