吉富罗非鱼对饲料中维生素B6的需要量

吴凡, 任春, 文华, 蒋明, 刘伟, 田娟, 杨长庚, 喻丽娟, 陆星

doi:10.3969/j.issn.1006-267x.2018.05.020

动物营养学报 >

2018 , Vol. 30 >Issue 5: 1781 - 1788

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.05.020

水产营养 Aquaculture Nutrition

吉富罗非鱼对饲料中维生素B₆的需要量

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1. 中国水产科学研究院长江水产研究所, 鱼类营养与饲料研究室, 武汉 430223;
2. 华中农业大学水产学院, 武汉 430070

吴凡(1981-),女,湖北荆州人,副研究员,硕士,从事鱼类营养与饲料研究。E-mail:wufan58@126.com

收稿日期: 2017-11-01

网络出版日期: 2018-05-06

基金资助

现代农业产业技术体系专项资金（CARS-46）；公益性行业（农业）科研专项经费（201003020）

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Dietary Vitamin B₆ Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

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1. Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2017-11-01

Online published: 2018-05-06

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摘要

本试验旨在研究饲料中维生素B₆含量对吉富罗非鱼生长性能、全鱼和肌肉营养组成及血清和肝脏生化指标的影响，以确定吉富罗非鱼对饲料中维生素B₆的需要量。选取360尾初始平均体重为（56.3±1.8）g的吉富罗非鱼，随机分成6个组，每组3个重复，每个重复20尾鱼。各组分别饲喂维生素B₆含量分别为0.3（对照组）、2.7、5.4、10.1、20.5和39.8 mg/kg的6种纯化饲料，进行10周的养殖试验。结果表明：1）随着饲料中维生B₆含量的升高（0.3~10.1 mg/kg），吉富罗非鱼的增重率显著增加（P<0.05），并在饲料中维生B₆含量达到10.1 mg/kg后趋于稳定；5.4、10.1、20.5和39.8 mg/kg组的饲料系数显著低于对照组和2.7 mg/kg组（P<0.05）；5.4和20.5 mg/kg组的肝体比显著高于对照组（P<0.05）。2）2.7、5.4、10.1、20.5和39.8 mg/kg组的全鱼粗蛋白质和粗灰分含量显著高于对照组（P<0.05）；10.1 mg/kg组的全鱼粗脂肪含量最低，显著低于对照组和2.7、5.4、39.8 mg/kg组（P<0.05）；5.4、10.1和20.5 mg/kg组的肌肉粗蛋白质含量显著高于对照组（P<0.05）。3）各组的血清葡萄糖、总蛋白含量和肝脏谷草转氨酶活性无显著差异（P>0.05）。20.5和39.8 mg/kg组的血清碱性磷酸酶活性显著高于对照组（P<0.05），10.1、39.8 mg/kg组的血清尿素氮含量显著高于对照组（P<0.05），10.1、20.5和39.8 mg/kg组的肝脏谷丙转氨酶活性显著高于对照组和2.7、5.4 mg/kg组（P<0.05）。综上，以增重率和肝脏谷丙转氨酶活性为评价指标，通过折线回归分析得到初始体重为（56.3±1.8）g的吉富罗非鱼对饲料中维生素B₆的需要量分别为9.22和10.11 mg/kg。

关键词： 吉富罗非鱼; 维生素B₆; 生长性能; 生化指标; 需要量

本文引用格式

吴凡, 任春, 文华, 蒋明, 刘伟, 田娟, 杨长庚, 喻丽娟, 陆星 . 吉富罗非鱼对饲料中维生素B₆的需要量[J]. 动物营养学报, 2018 , 30(5) : 1781 -1788 . DOI: 10.3969/j.issn.1006-267x.2018.05.020

Abstract

This experiment was conducted to estimate the effects of dietary vitamin B₆ content on growth performance, whole body and muscle nutritional composition, serum and liver biochemical indices of genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus), and to determine the dietary vitamin B₆ requirement of GIFT. A total of 360 GIFT with the average initial body weight of (56.3±1.8) g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The fish were fed six purified diets which containing 0.3 (control group), 2.7, 5.4, 10.1, 20.5 and 39.8 mg/kg vitamin B₆, respectively. The experiment lasted for 10 weeks. The results showed as follow:1) with the increased of dietary vitamin B₆ content (0.3 to 10.1 mg/kg), the weight gain rate of GIFT was significantly increased (P<0.05), and then reached a plateau when dietary vitamin B₆ content reached 10.1 mg/kg. The feed conversion ratio of GIFT in 5.4, 10.1, 20.5 and 39.8 mg/kg groups were significantly lower than those in control group and 2.7 mg/kg group (P<0.05). The hepatosomatic index in 5.4 and 20.5 mg/kg groups was significantly higher than that in control group (P<0.05). 2) The contents of crude protein and ash in whole body in 2.7, 5.4, 10.1, 20.5 and 39.8 mg/kg groups were significantly higher than those in control group (P<0.05). The 10.1 mg/kg group got the lowest crude lipid content, and was significantly lower than that in control group and 2.7, 5.4 and 39.8 mg/kg groups (P<0.05). The muscle crude protein content in 5.4,10.1 and 20.5 mg/kg groups was significantly higher than that in control group (P<0.05). 3) There were no significant effects on serum glucose, total protein contents and liver aspartate aminotransferase activity among all groups (P>0.05). The serum alkaline phosphatase activity in 20.5 and 39.8 mg/kg groups was significantly higher than that in control group (P<0.05), the serum urea nitrogen content in 10.1 and 39.8 mg/kg groups was significantly higher than that in control group (P<0.05), the liver alanine aminotransferase activity in 10.1, 20.5 and 39.8 mg/kg groups was significantly higher than that in control group and 2.7, 5.4 mg/kg groups (P<0.05). In summary, the broken-line regression analysis shows that using weight rate and liver alanine aminotransferase activity as response criteria, the optimal requirements of dietary vitamin B₆ for GIFT[initial body weight (56.3±1.8) g] are 9.22 and 10.11 mg/kg, respectively.

Key words： GIFT; vitamin B₆; growth performance; biochemical indices; requirement

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