分子营养 Molecular Nutrition

饲粮添加亮氨酸和谷氨酸对肥育猪肌肉脂肪酸组成和脂质代谢相关基因表达的影响

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  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 动物营养生理与代谢过程实验室, 畜禽养殖污染控制与资源化技术国家工程实验室, 长沙 410125;
    2. 华南农业大学, 动物科学学院, 广州 510642;
    3. 赢创德固赛(中国)投资有限公司, 北京 100600
胡诚军(1992-),男,江西新余人,硕士研究生,从事猪营养生理研究。E-mail:chengjhu@qq.com

收稿日期: 2017-11-02

  网络出版日期: 2018-05-06

基金资助

国家现代农业产业技术体系建设专项(CARS-35);国家自然科学基金面上项目(31572421);中央驻湘科研机构技术创新发展专项(2013TF3006)

Effects of Dietary Leucine and Glutamic Acid on Fatty Acid Composition and Expression of Lipid Metabolism-Related Genes in Muscle of Finishing Pigs

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  • 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Laboratory of Animal Nutritional Physiology and Metabolic Process, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    3. Evonik Degussa(China) Co., Ltd., Beijing 100600, China

Received date: 2017-11-02

  Online published: 2018-05-06

摘要

本试验旨在探讨饲粮添加亮氨酸和谷氨酸对肥育猪肌肉脂肪酸组成和脂质代谢相关基因表达的影响。选取平均体重为77 kg左右的三元杂交肥育猪60头,随机分为5个组,每组12头,公、母各占1/2。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加2.05%L-丙氨酸(等氮对照组)、1.00%亮氨酸+1.37%L-丙氨酸(亮氨酸组)、1.00%谷氨酸+1.44%L-丙氨酸(谷氨酸组)、1.00%亮氨酸+1.00%谷氨酸(亮氨酸+谷氨酸组)。饲喂60 d后屠宰采集肌肉样品,检测其中脂肪酸含量以及脂质代谢相关基因mRNA的相对表达量。结果表明:与对照组相比,在背最长肌中,亮氨酸组C18∶2n-6和C20∶1含量显著增加(P<0.05),C18∶0含量显著降低(P<0.05),谷氨酸组C14∶0和C16∶0含量显著降低(P<0.05),C17∶0和C18∶2n-6含量显著增加(P<0.05);在股二头肌中,亮氨酸组C16∶0含量显著降低(P<0.05),谷氨酸组C18∶2n-6含量显著增加(P<0.05)。与对照组相比,亮氨酸组背最长肌中脂肪酸转运蛋白1 mRNA的相对表达量显著上调(P<0.05),亮氨酸+谷氨酸组背最长肌和股二头肌中脂肪酸转运蛋白4 mRNA的相对表达量显著下调(P<0.05)。与等氮对照组相比,谷氨酸组和亮氨酸+谷氨酸组背最长肌中脂蛋白脂酶mRNA的相对表达量显著上调(P<0.05)。上述结果提示,饲粮添加1.00%亮氨酸或1.00%谷氨酸可调控肥育猪肌肉中脂肪酸组成及脂质代谢相关基因的表达。

本文引用格式

胡诚军, 张婷, 张涛, 印遇龙, 孔祥峰, 江青艳 . 饲粮添加亮氨酸和谷氨酸对肥育猪肌肉脂肪酸组成和脂质代谢相关基因表达的影响[J]. 动物营养学报, 2018 , 30(5) : 1798 -1806 . DOI: 10.3969/j.issn.1006-267x.2018.05.022

Abstract

This study was conducted to investigate the effects of dietary leucine (Leu) and glutamic acid (Glu) on fatty acid composition and expression of lipid metabolism-related genes in muscle of finishing pigs. Sixty Duroc×Large White×Landrace pigs with an average body weight about 77 kg were randomly assigned to five groups, and each group had 12 pigs (male:female=1:1). The pigs in control group were fed a basal diet, and those in the experiment groups were fed the basal diet supplemented with 2.05% L-alanine (iso-nitrogenous control group), 1.00% Leu+1.37% L-alanine (Leu group), 1.00% Glu+1.44% L-alanine (Glu group) and 1.00% Leu+1.00% Glu (Leu+Glu group), respectively. After feeding 60 days, the pigs were slaughtered and the muscle samples were collected to measure the fatty acid composition and mRNA relative expression levels of genes related to lipid metabolism. The results showed that the contents of C18:2n-6 and C20:1 in longissimus dorsi muscle in Leu group were significantly increased (P<0.05), whereas the content of C18:0 was significantly decreased compared with the control group (P<0.05); the contents of C14:0 and C16:0 in longissimus dorsi muscle in Glu group were significantly decreased (P<0.05), whereas the contents of C17:0 and C18:2n-6 were significantly increased (P<0.05). In the biceps femoris muscle, compared with the control group, the content of C16:0 in Leu group was significantly decreased (P<0.05), while the content of C18:2n-6 in Glu group was significantly increased (P<0.05). Compared with the control group, the mRNA expression level of fatty-acidtransport protein 1 in longissimus dorsi muscle was significantly up-regulated in Leu group (P<0.05), whereas the mRNA expression level of fatty-acidtransport protein 4 in longissimus dorsi and biceps femoris muscles was significantly down-regulated in Leu+Glu group (P<0.05). Compared with the iso-nitrogenous control group, the mRNA expression level of lipoprotein lipase in longissimus dorsi muscle was significantly up-regulated in Glu and Leu+Glu groups (P<0.05). These findings indicate that diets supplemented with 1.00% Leu or 1.00% Glu can regulate the fatty acid composition and the expression of genes related to lipid metabolism in muscle of finishing pigs.

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