血根碱对断奶仔猪生长性能、肠道形态结构及小肠黏膜免疫功能的影响

陈家顺, 康宝聚, 曾建国, 胡海波, 赵玉蓉, 陈柳, 姚康, 符晨星

doi:10.3969/j.issn.1006-267x.2018.05.027

动物营养学报 >

2018 , Vol. 30 >Issue 5: 1845 - 1853

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.05.027

饲料营养 Feed science and technology

血根碱对断奶仔猪生长性能、肠道形态结构及小肠黏膜免疫功能的影响

展开

1. 湖南农业大学动物科学技术学院, 长沙 410128;
2. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 长沙 410125;
3. 湖南农业大学, 湖南省植物功能成分利用协同创新中心, 长沙 410128;
4. 湖南美可达生物资源有限公司, 长沙 410128;
5. 浏阳市畜牧兽医水产局, 浏阳 410300

陈家顺(1988-),男,安徽亳州人,博士研究生,从事单胃动物营养与饲料研究。E-mail:730273902@qq.com

收稿日期: 2017-10-18

网络出版日期: 2018-05-06

基金资助

国家自然科学基金青年科学基金项目（31702126）；湖南省研究生科研创新项目（CX2017B348）；湖南省教育厅科学研究项目优秀青年项目（17B121）；湖南省"湖湘青年科技创新人才"项目（2015RS4053）；中科院"百人计划"项目

收起

Effects of Dietary Sanguinarine on Growth Performance, Intestinal Mucosal Morphology and Immune Function of Small Intestinal Mucosa of Weaned Piglets

Expand

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Hunan Collaborative Innovation Center for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China;
4. Hunan Micolt Bioresource Co., Ltd., Changsha 410128, China;
5. Bureau of Animal Husbandry and Veterinary and Aquaculture in Liuyang City, Liuyang 410300, China

Received date: 2017-10-18

Online published: 2018-05-06

Fold

摘要

本试验旨在研究血根碱（SAN）对断奶仔猪生长性能、肠道形态结构及小肠黏膜免疫功能的影响。试验选取23日龄平均体重为（6.55±0.18）kg的健康"杜×长×白"三元杂交断奶仔猪36头，按体重相近、性别比例相同原则随机分为3组，分别饲喂基础饲粮（对照组）、基础饲粮+75 mg/kg金霉素（抗生素组）、基础饲粮+50 mg/kg SAN（SAN组），每组12个重复，每个重复1头猪，试验期21 d。结果表明：1）与对照组相比，SAN组和抗生素组的平均日增重和平均日采食量显著提高（P<0.05），料重比和腹泻率显著降低（P<0.05）；但SAN组与抗生素组间以上生长性能指标均无显著性差异（P>0.05）。2）与对照组相比，SAN组和抗生素组的十二指肠和空肠绒毛高度、绒毛高度/隐窝深度（V/C）值显著或极显著升高（P<0.05或P<0.01），但SAN组和抗生素组间以上指标均无显著差异（P>0.05）；而SAN组回肠绒毛高度、V/C值显著高于其他2组（P<0.05）。3）与对照组相比，SAN和抗生素组的十二指肠、空肠和回肠黏膜免疫球蛋白A、免疫球蛋白G和免疫球蛋白M含量均显著或极显著升高（除十二指肠黏膜免疫球蛋白G和空肠黏膜免疫球蛋白M外）（P<0.05或P<0.01）；但SAN组和抗生素组间以上指标均无显著差异（P>0.05）。由此可见，饲粮中添加SAN可以改善肠黏膜形态，提高小肠黏膜免疫功能，从而提高断奶仔猪的生长性能，达到与添加抗生素相当的效果。

关键词： 血根碱; 断奶仔猪; 生长性能; 肠道形态结构; 免疫球蛋白

本文引用格式

陈家顺, 康宝聚, 曾建国, 胡海波, 赵玉蓉, 陈柳, 姚康, 符晨星 . 血根碱对断奶仔猪生长性能、肠道形态结构及小肠黏膜免疫功能的影响[J]. 动物营养学报, 2018 , 30(5) : 1845 -1853 . DOI: 10.3969/j.issn.1006-267x.2018.05.027

Abstract

This experiment was conducted to study the effects of dietary sanguinarine (SAN) on growth performance, intestinal mucosal morphology and immune function of small intestinal mucosa of weaned piglets. Thirty-six "Duroc×Landrace×Yorkshire" hybrid pigs with initial weight of (6.55±0.18) kg were randomly divided into three groups with twelve replicates per group and one pig per replicate according to similar body weight and the same ratio of gender. The pigs in the control group were fed a basal diet, those in antibiotic group were fed the basal diet supplemented with 75 mg/kg chlortetracycline, and the others in SAN group were fed the basal diet supplemented with 50 mg/kg sanguinarine. The experiment lasted for 21 days. The results showed as follows:1) compared with the control group, the average daily gain and the average daily feed intake were significantly increased (P<0.05), and the ratio of feed to gain and diarrhea rate were significantly decreased (P<0.05) in SAN group and antibiotic group. But there were no significant differences in above growth performance indices between SAN group and antibiotic group (P>0.05). 2) Compared with the control group, the villus height and the ratio of villus height to crypt depth (V/C) of duodenum and jejunum were significantly increased (P<0.05 or P<0.01) in SAN group and antibiotic group, and no significant differences were found in above indices between SAN group and antibiotic group (P>0.05), while the villus height and V/C of ileum in SAN group were significantly higher than those in the other two groups (P<0.05). 3) Compared with the control group, the contents of immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) in duodenum, jejunum and ileum in SAN group and antibiotic group were significantly increased (P<0.05 or P<0.01) except the IgG content in duodenum mucosa and the IgM content in jejunum mucosa. But no significant differences were found in above indices between SAN group and antibiotic group (P>0.05). In conclusion, dietary sanguinarine with the same effects of antibiotic can improve intestinal mucosal morphology, and increase small intestinal mucosal immune function, consequently improve the growth performance of weaned piglets.

Key words： sanguinarine; weaned piglets; growth performance; intestinal mucosal morphology; immunoglobulin

参考文献

[1] 李维,冯培刚,王恬.仔猪断奶应激及其营养调控[J].家畜生态学报,2007,28(6):1-4.

[2] 王斌星,王蜀金,郭春华,等.酿酒酵母发酵液对断奶仔猪生长性能、小肠发育及小肠黏膜免疫功能的影响[J].动物营养学报,2016,28(12):4014-4022.

[3] 王彪,张慧林,刘小林.天然植物提取物在畜禽生产中的应用研究[J].畜牧兽医杂志,2010,29(1):37-39.

[4] 楚维斌,史彬林,红雷,等.抗生素在畜禽生产中的应用·危害及科学使用[J].安徽农业科学,2015(19):128-130.

[5] BLANKR,MVLLER-SIEGWARDT B,WOLFFRAMS,et al.Sanguinarine does not influence availability or metabolism of tryptophan in pigs[J].Livestock Science,2010,134(1/2/3):24-26.

[6] 王静慧,韩剑众,曲道峰.血根碱体外抑菌作用及其对细菌生物被膜的影响[J].中国畜牧杂志,2012,48(19):67-70.

[7] WEERASINGHE P,HALLOCK S,TANG S C,et al.Role of Bcl-2 family proteins and caspase-3 in sanguinarine-induced bimodal cell death[J].Cell Biology and Toxicology,2001,17(6):371-381.

[8] HUSSAIN A R,AL-JOHAH N A,SIRAJ A K,et al.Sanguinarine-dependent induction of apoptosis in primary effusion lymphoma cells[J].Cancer Research,2007,67(8):3888-3897.

[9] MATKAR S S,WRISCHNIK L A,HELLMANN-BLUMBERG U.Sanguinarine causes DNA damage and p53-independent cell death in human colon cancer cell lines[J].Chemico-Biological Interactions,2008,172(1):63-71.

[10] ZHANG C,LING F,CHI C,et al.Effects of praziquantel and sanguinarine on expression of immune genes and susceptibility to Aeromonas hydrophila in goldfish (Carassius auratus) infected with Dactylogyrus intermedius[J].Fish & Shellfish Immunology,2013,35(4):1301-1308.

[11] BOJJIREDDY N,SINHA R K,PANDA D,et al.Sanguinarine suppresses IgE induced inflammatory responses through inhibition of type Ⅱ PtdIns 4-kinase(s)[J].Archives of Biochemistry and Biophysics,2013,537(2):192-197.

[12] YUAN L,RONG J,MA Z G,et al.Sanguinarine inhibits angiotensin Ⅱ-induced apoptosis in H9c2 cardiac cells via restoring reactive oxygen species-mediated decreases in the mitochondrial membrane potential[J].Molecular Medicine Reports,2015,12(3):3400-3408.

[13] KOSINA P,WALTEROVÁ D,ULRICHOVA J,et al.Sanguinarine and chelerythrine:assessment of safety on pigs in ninety days feeding experiment[J].Food and Chemical Toxicology,2004,42(1):85-91.

[14] LEE K W,KIM J S,OH S T,et al.Effects of dietary sanguinarine on growth performance,relative organ weight,cecal microflora,serum cholesterol level and meat quality in broiler chickens[J].The Journal of Poultry Science,2015,52(1):15-22.

[15] 刘靖.博落回生物碱对黄羽肉鸡生长的影响[D].硕士学位论文.长沙:湖南农业大学,2010.

[16] 云龙.血根碱制剂对黄羽肉鸡生长性能及抗氧化功能的影响[D].硕士学位论文.长沙:湖南农业大学,2016.

[17] NRC.Nutrient requirements of swine[S].11th ed.Washington,D.C.:National Academy Press,2012.

[18] 贺琴,王自蕊,游金明,等.酵母壁多糖对断奶仔猪生长性能和小肠黏膜形态结构的影响[J].动物营养学报,2016,28(11):3536-3541.

[19] 张卫兵,张蓉,屠焰,等.血根碱的作用机制及其在动物营养上的应用效果[J].动物营养学报,2017,29(1):27-33.

[20] VE ?E ?A R,KLEJDUS B,KOSINA P,et al.Disposition of sanguinarine in the rat[J].Xenobiotica,2007,37(5):549-558.

[21] 饶华,蔡鹏,周锡红,等.博落回提取物对断奶仔猪生长性能的影响[J].中国兽药杂志,2009,43(11):42-45.

[22] 戴波.血根碱对断奶仔猪生产性能的影响及腹泻防治效果的研究[D].硕士学位论文.长沙:湖南农业大学,2015.

[23] KANTAS D,PAPATSIROS V G,TASSIS P D,et al.The effect of a natural feed additive (Macleaya cordata),containing sanguinarine,on the performance and health status of weaning pigs[J].Animal Science Journal,2015,86(1):92-98.

[24] VIEIRA S L,BERRES J,REIS R N,et al.Studies with sanguinarine like alkaloids as feed additive in broiler diets[J].Brazilian Journal of Poultry Science,2008,10(1):67-71.

[25] DRSATA J,ULRICHOVÁ J,WALTEROVÁ D.Sanguinarine and chelerythrine as inhibitors of aromatic amino acid decarboxylase[J].Journal of Enzyme Inhibition,1996,10(4):231-237.

[26] 荀文娟,周汉林,侯冠彧,等.姜黄素对早期断奶仔猪回肠黏膜形态、紧密连接蛋白和炎性因子基因表达以及血清免疫球蛋白水平的影响[J].动物营养学报,2016,28(3):826-833.

[27] 苏家宜,孔祥峰,李华伟,等.芪楂口服液药渣对断奶仔猪生长性能和肠道健康的影响[J].动物营养学报,2017,29(5):1730-1738.

[28] 贺喜,刘靖,张石蕊,等.博落回生物碱对黄羽肉鸡生产性能、内脏器官及肠道健康的影响[C]//第六次全国饲料营养学术研讨会论文集.杨凌:中国畜牧兽医学会,2010.

[29] 何夏阳.血根碱对断奶仔猪生长性能、养分消化率和血液生化指标的影响[D].硕士学位论文.长沙:湖南农业大学,2010.

[30] 李杰,伍树松,熊兴耀,等.博落回生物碱对猪肠上皮细胞增殖的影响[J].动物营养学报,2014,26(6):1632-1637.

[31] YAKHKESHI S,RAHIMI S,NASERI K G.The effects of comparison of herbal extracts,antibiotic,probiotic and organic acid on serum lipids,immune response,GIT microbial population,intestinal morphology and performance of broilers[J].Lunión Médicale Du Canada,2011,113(10):826-828.

[32] WINK M,SCHMELLER T,LATZ-BRVNING B.Modes of action of allelochemical alkaloids:interaction with neuroreceptors,DNA,and other molecular targets[J].Journal of Chemical Ecology,1998,24(11):1881-1937.

[33] 孙文霞,袁仕善,黄琼瑶,等.血水草生物碱及其血根碱抑菌作用的研究[J].实用预防医学,2010,17(9):1864-1866.

[34] HERRERA-MATA H,ROSAS-ROMERO A,OSCAR C V.Biological activity of "sanguinaria" (Justicia secunda) extracts[J].Pharmaceutical Biology,2008,40(3):206-212.

[35] 郭小清,唐莉苹,聂建超,等.博落回的药理作用及其在动物保健中的应用[J].中国动物保健,2005(5):34-35.

[36] 杨军,王静,刘信顺,等.博落回的药效研究[J].中药材,1999(2):82-85.

[37] 满意,张春勇,李美荃,等.博落回提取物对早期断奶仔猪生长性能和血清免疫参数的影响[J].动物营养学报,2013,25(1):126-132.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们