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脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮对癌细胞的促增殖和促迁移作用

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  • 1. 中国农业科学院农业质量标准与检测技术研究所, 农业部农产品质量安全重点实验室, 北京 100081;
    2. 北京农学院动物科学技术学院, 北京 102206
邱思奇(1991-),女,湖北应城人,硕士研究生,从事免疫药理研究。E-mail:qiusiqi1005@sina.com

收稿日期: 2017-11-14

  网络出版日期: 2018-05-06

基金资助

中央级公益性科研院所基本科研业务费专项(1610072006014);2017年北京农学院学位与研究生教育改革与发展项目(YJS048,YJS020);2017年北京高等学校高水平人才交叉培养"实培计划"项目

Effects of Deoxynivalenol and Zearalenone on Promoting Proliferation and Promoting Migration of Cancer Cells

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  • 1. Key Laboratory of Agro-Food Safety and Quality, Ministry of Agriculture, Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received date: 2017-11-14

  Online published: 2018-05-06

摘要

本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)和玉米赤霉烯酮(ZEN)对人结直肠腺癌细胞(HCT116细胞)、人肺癌细胞(A549细胞)、人肝癌细胞(HepG2细胞)的促增殖和促迁移作用。设空白对照组,并以赭曲霉毒素A(OTA)作为阳性对照,采用噻唑蓝(MTT)比色法和细胞划痕愈合试验分别检测细胞活力和细胞迁移能力。结果表明:与空白对照组相比,0.080、0.400 μmol/L OTA能显著或极显著增强HCT116、A549和HepG2细胞活力(P<0.05或P<0.01),50.000 μmol/L OTA则能极显著降低HCT116、A549和HepG2细胞活力(P<0.01);DON在低浓度(0.016、0.080 μmol/L)时对HCT116和A549细胞活力无显著影响(P > 0.05),但能极显著增强HepG2细胞活力(P<0.01),而在DON中高浓度(2.000、10.000、50.000 μmol/L)时则能极显著降低HCT116、A549和HepG2细胞活力(P<0.01);ZEN浓度在0.016~50.000 μmol/L时能显著或极显著增强HepG2细胞活力(P<0.05或P<0.01),ZEN在高浓度(50.000 μmol/L)时能极显著降低HCT116和A549细胞活力(P<0.01)。细胞划痕愈合试验结果表明,与空白对照组相比,1、10 nmol/L OTA、DON和ZEN作用24、48 h能极显著促进HepG2细胞迁移(P<0.01)。由此可见,在0.016~50.000 μmol/L浓度内,DON和ZEN对HepG2细胞有显著的促进增殖和迁移的作用,表明DON和ZEN在HepG2细胞系上具有潜在的致癌性。

关键词: 霉菌毒素; 细胞; 增殖; 迁移

本文引用格式

邱思奇, 徐贞贞, 沈红, 陈爱亮, 杨曙明, 晁雨竹 . 脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮对癌细胞的促增殖和促迁移作用[J]. 动物营养学报, 2018 , 30(5) : 1988 -1995 . DOI: 10.3969/j.issn.1006-267x.2018.05.044

Abstract

This experiment was conducted to study the effects of deoxynivalenol (DON) and zearalenone (ZEN) on promoting proliferation and promoting migration of human colon carcinoma cell (HCT116 cell), human lung cancer cell (A549 cell) and human hepatocellular carcinoma cell (HepG2 cell). The experiment set a blank control group, and ochratoxin A (OTA) was set up as a positive control, methyl thiazolyl tetrazolium (MTT) colorimetry and cell wound healing experiment were used to determine cell viability and cell migration activity. The results showed that compared with the blank control group, 0.080 and 0.400 μmol/L OTA could significantly enhance the viability of HCT116, A549 and HepG2 cells (P<0.05 or P<0.01), 50.000 μmol/L OTA could significantly decrease the viability of HCT116, A549 and HepG2 cells (P<0.01); DON had no effect on the viability of HCT116 and A549 cells at low concentration (0.016 and 0.080 μmol/L) (P > 0.05), but DON could significantly enhance the viability HepG2 cell at low concentration (0.016 and 0.080 μmol/L) (P<0.01), and DON could significantly decrease the viability of HCT116, A549 and HepG2 cells at middle and high concentration (2.000, 10.000 and 50.000 μmol/L) (P<0.01); ZEN could significantly enhance the viability of HepG2 cell at the concentration of 0.016 to 50.000 μmol/L (P<0.05 or P<0.01), ZEN could significantly decrease the viability of HCT116 and A549 cells at high concentration (50.000 μmol/L) (P<0.01). The cell wound healing experiment results showed that compared with the blank control group, 1 and 10 nmol/L OTA, DON and ZEN could significantly promote the migration of HepG2 cell at 24 and 48 h (P<0.01). In conclusion, at the concentration of 0.016 to 50.000 μmol/L, DON and ZEN significantly promote proliferation and migration of HepG2 cell, which suggests that DON and ZEN exhibit carcinogenesis-like properties in HepG2 cell.

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