动物营养学报 >

2018 , Vol. 30 >Issue 8: 3017 - 3025

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.018

反刍动物营养 Ruminant nutrition

不同生理阶段荷斯坦奶牛瘤胃细菌多样性研究

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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古自治区农牧业科学院动物营养研究所, 呼和浩特 010031;
    3. 内蒙古优然牧业有限责任公司, 呼和浩特 010107
李子健(1992-),男,河北秦皇岛人,硕士研究生,从事反刍动物营养与瘤胃微生态研究。E-mail:1220838116@qq.com

收稿日期: 2018-02-03

  网络出版日期: 2018-08-18

基金资助

现代农业（奶牛）产业技术体系建设专项资金（CARS-37）

收起

Rumen Bacteria Diversity in Holstein Dairy Cows at Different Physiological Phases

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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Animal Nutrition Institute, Agriculture and Animal Husbandry Academy of Inner Mongolia, Hohhot 010031, China;
    3. Inner Mongolia Youran Co., Ltd., Hohhot 010107, China

Received date: 2018-02-03

  Online published: 2018-08-18

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摘要

本试验旨在探究不同生理阶段荷斯坦奶牛瘤胃细菌多样性。选取处于围产前期、泌乳前期、泌乳中期和泌乳后期的经产荷斯坦奶牛各4头，共16头，采集瘤胃液用于测定瘤胃发酵参数和提取微生物DNA，采用Illumina Miseq PE300平台测定瘤胃细菌组成。结果表明：1）围产前期奶牛瘤胃微生物蛋白、乙酸、丁酸、总挥发性脂肪酸浓度与乙酸/丙酸显著高于泌乳期（P<0.05）。围产前期奶牛瘤胃丙酸浓度显著低于泌乳期（P<0.05）。2）在门的水平上，围产前期奶牛瘤胃内的优势菌门为拟杆菌门和厚壁菌门，泌乳期奶牛瘤胃内的优势菌门则为拟杆菌门、变形菌门和厚壁菌门。围产前期奶牛瘤胃内的拟杆菌门、SR1细菌、蓝菌门、柔膜菌门、TM7细菌和纤维杆菌门的相对丰度显著高于泌乳期（P<0.05），而泌乳期各阶段之间差异不显著（P>0.05）；围产前期奶牛瘤胃内的变形菌门的相对丰度显著低于泌乳期（P<0.05）。3）在科的水平上，围产前期奶牛瘤胃内的优势菌科为普雷沃氏菌科，而泌乳期奶牛瘤胃内的优势菌科为琥珀酸弧菌科和普雷沃氏菌科。围产前期奶牛瘤胃内的普雷沃氏菌科、瘤胃菌科、理研菌科、BS11细菌、RF16细菌、SR1细菌、Gastranaerophilales、克里斯滕森菌科、TM7细菌、RF9细菌和纤维杆菌科的相对丰度显著高于泌乳期（P<0.05）；围产前期奶牛瘤胃内的琥珀酸弧菌科、毛螺菌科和韦荣球菌科则显著低于泌乳期（P<0.05）。综上得出，奶牛从围产前期进入到泌乳期，瘤胃细菌多样性显著降低；泌乳期各阶段瘤胃细菌组成差异较小。

关键词： 荷斯坦奶牛; 生理阶段; 瘤胃细菌; 多样性

本文引用格式

李子健, 李大彪, 高民, 王典, 兰儒冰 . 不同生理阶段荷斯坦奶牛瘤胃细菌多样性研究[J]. 动物营养学报, 2018 , 30(8) : 3017 -3025 . DOI: 10.3969/j.issn.1006-267x.2018.08.018

Abstract

This study aimed at investigating the rumen bacterial diversity in Holstein dairy cows at different physiological phases. Sixteen cows were chose and divided into 4 groups according to their physiological phases which were early prenatal phase, early lactation phase, medium lactation phase and late lactation phase. Collecting the fluid phase of ruminal fluid to determined the rumen fermentation parameters and extracted the rumen microbial DNA. The Illumina Miseq PE300 platform was used to determine the rumen bacterial composition. The results showed as follows:1) the concentrations of microbial protein, acetic acid, butyric acid and total volatile fatty acid and acetic acid/propionic acid in rumen of cows in early prenatal phase were significantly higher than those in lactation phase (P<0.05), the rumen propionic acid concentration cows in early prenatal phase was significantly lower than that in lactation phase (P<0.05). 2) At the level of phyla, the dominant bacteria in rumen of cows in early prenatal phase were Bacteroides and Firmicutes, the dominant bacteria in rumen of cows in lactation phase were Bacteroidetes, Proteobacteria and Firmicutes. The relative abundances of Bacteroidetes, SR1 bacteria, Cyanobacteria, TM7 bacteria, and Fibrobacter in rumen of cows in early prenatal phase were significantly higher than those in lactation phase (P<0.05), while there was no significant difference among all lactation phases (P>0.05). In contrast, the relative abundance of Proteobacteria in rumen of cows in early prenatal phase was significantly lower than that in lactation phase (P<0.05). 3). At the level of family, the dominant bacteria in rumen of cows in early prenatal phase were Prevotellaceae, while the dominant bacteria in rumen of cows in lactation phase were Succinivibrionaceae and Prevotellaceae. The relative abundances of Prevotella, Ruminococcaceae, Rikenellaceae, BS11 bacteria, RF16 bacteria, SR1 bacteria, Gastranaerophilales, Christensenellaceae, TM7 bacteria, RF9 bacteria and Fibrobacteria in rumen of cows in early prenatal phase were significantly higher than those in lactation phase (P<0.05). The relative abundances of Succinivibrionaceae, Lachnospiraceae and Veillonellaceae in rumen of cows in early prenatal phase were significantly lower than those in lactation phase (P<0.05). In conclusion, the diversity of rumen bacteria in dairy cows significantly decrease from early prenatal phase to lactation phase, but there is little difference in the composition of rumen bacteria among all lactation phases.

Key words： Holstein cows; physiological phases; rumen bacteria; diversity

参考文献

[1] JENKINS T C,WALLACE J R,MOATE P J,et al.Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem[J].Journal of Animal Science,2008,86(2):397-412.  

[2] BRULC J M,ANTONOPOULOS D A,MILLER M E B,et al.Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(6):1948-1953.  

[3] HOBSEN P N,STEWART C S.The rumen microbial ecosystem[M].Netherlands:Spring Science & Business Media,1997.

[4] 郭伟.不同年龄阶段山羊瘤胃古菌,细菌菌群结构组成[D].硕士学位论文.雅安:四川农业大学,2015:38-64.

[5] JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The ISME Journal,2013,7(6):1069-1079.  

[6] 高凤.奶牛肠道微生物群落结构与多样性研究[D].硕士学位论文.邯郸:河北工程大学,2017:23-48.

[7] 董瑞阳.粗饲料组合对泌乳牛与干奶牛甲烷产量、瘤胃发酵模式、及微生物菌群的影响[D].硕士学位论文.郑州:河南农业大学,2014:32-33.

[8] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:The National Academies Press,2001:65-67.

[9] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:52-147.

[10] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41.

[11] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72:72-248.

[12] 秦为琳.应用气相色谱测定瘤胃挥发性脂肪酸方法的研究改进[J].南京农学院学报,1983,5(4):110-116.

[13] KLINE L W,KARPINSKI E.Progesterone inhibits gallbladder motility through multiple signaling pathways[J].Steroids,2005,70(7):673-679.

[14] COLLADO M C,ISOLAURI E,LAITINEN K,et al.Distinct composition of gut microbiota during pregnancy in overweight and normal-weight women[J].The American Journal of Clinical Nutrition,2008,88(6):894-899.

[15] KOREN O,GOODRICH J K,CULLENDER T C,et al.Host remodeling of the gut microbiome and metabolic changes during pregnancy[J].Cell,2012,150(3):470-480.  

[16] KONG X F,JI Y J,LI H W,et al.Colonic luminal microbiota and bacterial metabolite composition in pregnant Huanjiang mini-pigs:effects of food composition at different times of pregnancy[J].Scientific Reports,2016,6:37224.

[17] JOST T,LACROIX C,BRAEGGER C,et al.Stability of the maternal gut microbiota during late pregnancy and early lactation[J].Current Microbiology,2014,68(9):419-427.

[18] 孙云章,毛胜勇,姚文,等.不同精粗比底物下瘤胃真菌和纤维降解细菌共培养发酵特性及菌群变化[J].微生物学报,2006,46(3):422-426.

[19] 王海荣.不同日粮精粗比及氮源对绵羊瘤胃纤维降解菌群和纤维物质降解的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2006:2-3.

[20] MAO S Y,ZHANG R Y,WANG D S,et al.Impact of subacute ruminal acidosis (SARA) adaptation on rumen microbiota in dairy cattle using pyrosequencing[J].Anaerobe,2013,24:12-19.

[21] KOCHERGINSKAYA S A,AMINOV R I,WHITE B A.Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis,random sequencing,and statistical ecology approaches[J].Anaerobe,2001,7(3):119-134.  

[22] 林波,梁辛,李丽莉,等.饲粮精粗比对泌乳水牛瘤胃细菌和甲烷菌区系的影响[J].动物营养学报,2016,28(10):3101-3109.

[23] MATSUI H,OGATA K,TAJIMA K,et al.Phenotypic characterization of polysaccharidases produced by four Prevotella type strains[J].Current Microbiology,2000,41(1):45-49.  
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